Restriction map for
pFN21AB9591
(Linear) (Six-base) MAPSORT of: KIBB9591.seq Check: 20 from: 1 to: 1767
KIBB9591.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AatII G_ACGT'C
Cuts at: 0 143 1767
Size: 143 1624
AccI GT'mk_AC
Cuts at: 0 938 1767
Size: 938 829
Acc65I G'GTAC_C
Cuts at: 0 1351 1767
Size: 1351 416
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 1177 1767
Size: 685 492 590
Fragments arranged by size:
685 590 492
AfeI AGC'GCT
Cuts at: 0 673 1767
Size: 673 1094
AflII C'TTAA_G
Cuts at: 0 1330 1767
Size: 1330 437
AflIII A'CryG_T
Cuts at: 0 1688 1767
Size: 1688 79
AleI CACnn'nnGTG
Cuts at: 0 477 1767
Size: 477 1290
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 475 495 1218 1767
Size: 475 20 723 549
Fragments arranged by size:
723 549 475 20
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 13 45 1767
Size: 13 32 1722
Fragments arranged by size:
1722 32 13
AlwNI CAG_nnn'CTG
Cuts at: 0 857 1462 1748 1767
Size: 857 605 286 19
Fragments arranged by size:
857 605 286 19
ApaLI G'TGCA_C
Cuts at: 0 480 1767
Size: 480 1287
ApoI r'AATT_y
Cuts at: 0 1504 1528 1767
Size: 1504 24 239
Fragments arranged by size:
1504 239 24
AvaI C'yCGr_G
Cuts at: 0 323 1767
Size: 323 1444
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1343 1376 1767
Size: 1343 33 391
Fragments arranged by size:
1343 391 33
BanI G'GyrC_C
Cuts at: 0 1351 1767
Size: 1351 416
BciVI GTATCCnnnnn_n'
Cuts at: 0 1627 1767
Size: 1627 140
BfrBI ATG'CAT
Cuts at: 0 403 1767
Size: 403 1364
Bme1580I G_kGCm'C
Cuts at: 0 484 864 1187 1767
Size: 484 380 323 580
Fragments arranged by size:
580 484 380 323
BmgBI CAC'GTC
Cuts at: 0 1689 1767
Size: 1689 78
BmrI ACTGGGnnnn_n'
Cuts at: 0 1043 1767
Size: 1043 724
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 541 625 1767
Size: 400 141 84 1142
Fragments arranged by size:
1142 400 141 84
BsaI GGTCTCn'nnnn_
Cuts at: 0 1065 1767
Size: 1065 702
BsaAI yAC'GTr
Cuts at: 0 1623 1767
Size: 1623 144
BsaHI Gr'CG_yC
Cuts at: 0 140 1767
Size: 140 1627
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 61 91 498 528 1767
Size: 61 30 407 30 1239
Fragments arranged by size:
1239 407 61 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 309 1111 1767
Size: 309 802 656
Fragments arranged by size:
802 656 309
BseYI C'CCAG_C
Cuts at: 0 979 1436 1467 1767
Size: 979 457 31 300
Fragments arranged by size:
979 457 300 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 1075 1767
Size: 428 647 692
Fragments arranged by size:
692 647 428
BsiEI CG_ry'CG
Cuts at: 0 1193 1767
Size: 1193 574
BsiHKAI G_wGCw'C
Cuts at: 0 484 1767
Size: 484 1283
BsmI GAATG_Cn'
Cuts at: 0 1643 1767
Size: 1643 124
BsmBI CGTCTCn'nnnn_
Cuts at: 0 133 1767
Size: 133 1634
Bsp1286I G_dGCh'C
Cuts at: 0 484 864 1187 1767
Size: 484 380 323 580
Fragments arranged by size:
580 484 380 323
BspHI T'CATG_A
Cuts at: 0 1365 1767
Size: 1365 402
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 437 1227 1767
Size: 437 790 540
Fragments arranged by size:
790 540 437
BsrFI r'CCGG_y
Cuts at: 0 1422 1767
Size: 1422 345
BsrGI T'GTAC_A
Cuts at: 0 1025 1767
Size: 1025 742
BssSI C'ACGA_G
Cuts at: 0 1093 1767
Size: 1093 674
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1589 1767
Size: 1589 178
BstEII G'GTnAC_C
Cuts at: 0 846 1767
Size: 846 921
BstXI CCAn_nnnn'nTGG
Cuts at: 0 447 1125 1767
Size: 447 678 642
Fragments arranged by size:
678 642 447
BtgI C'CryG_G
Cuts at: 0 752 1140 1274 1767
Size: 752 388 134 493
Fragments arranged by size:
752 493 388 134
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1665 1767
Size: 1665 102
BtsI GCAGTG_nn'
Cuts at: 0 191 1161 1767
Size: 191 970 606
Fragments arranged by size:
970 606 191
DrdI GACnn_nn'nnGTC
Cuts at: 0 256 1767
Size: 256 1511
EaeI y'GGCC_r
Cuts at: 0 749 879 1127 1137 1190 1424 1767
Size: 749 130 248 10 53 234 343
Fragments arranged by size:
749 343 248 234 130 53 10
EagI C'GGCC_G
Cuts at: 0 1190 1767
Size: 1190 577
EarI CTCTTCn'nnn_
Cuts at: 0 136 593 794 895 1767
Size: 136 457 201 101 872
Fragments arranged by size:
872 457 201 136 101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 541 625 685 1177 1767
Size: 400 141 84 60 492 590
Fragments arranged by size:
590 492 400 141 84 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1093 1767
Size: 1093 674
EcoO109I rG'GnC_Cy
Cuts at: 0 164 1767
Size: 164 1603
EcoRI G'AATT_C
Cuts at: 0 1504 1767
Size: 1504 263
HaeII r_GCGC'y
Cuts at: 0 675 1767
Size: 675 1092
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 498 530 1087 1119 1767
Size: 498 32 557 32 648
Fragments arranged by size:
648 557 498 32 32
HincII GTy'rAC
Cuts at: 0 261 1767
Size: 261 1506
KpnI G_GTAC'C
Cuts at: 0 1355 1767
Size: 1355 412
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1608 1702 1767
Size: 1608 94 65
Fragments arranged by size:
1608 94 65
MscI TGG'CCA
Cuts at: 0 751 881 1129 1139 1767
Size: 751 130 248 10 628
Fragments arranged by size:
751 628 248 130 10
MslI CAynn'nnrTG
Cuts at: 0 477 577 1123 1767
Size: 477 100 546 644
Fragments arranged by size:
644 546 477 100
MspA1I CmG'CkG
Cuts at: 0 44 561 1462 1767
Size: 44 517 901 305
Fragments arranged by size:
901 517 305 44
NcoI C'CATG_G
Cuts at: 0 752 1140 1274 1767
Size: 752 388 134 493
Fragments arranged by size:
752 493 388 134
NsiI A_TGCA'T
Cuts at: 0 405 1767
Size: 405 1362
NspI r_CATG'y
Cuts at: 0 200 378 407 456 1007 1767
Size: 200 178 29 49 551 760
Fragments arranged by size:
760 551 200 178 49 29
PflMI CCAn_nnn'nTGG
Cuts at: 0 573 1599 1767
Size: 573 1026 168
Fragments arranged by size:
1026 573 168
PfoI T'CCnGG_A
Cuts at: 0 359 395 416 1767
Size: 359 36 21 1351
Fragments arranged by size:
1351 359 36 21
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 205 237 1767
Size: 205 32 1530
Fragments arranged by size:
1530 205 32
PpuMI rG'GwC_Cy
Cuts at: 0 164 1767
Size: 164 1603
PsiI TTA'TAA
Cuts at: 0 1526 1767
Size: 1526 241
PstI C_TGCA'G
Cuts at: 0 833 1767
Size: 833 934
PvuII CAG'CTG
Cuts at: 0 561 1462 1767
Size: 561 901 305
Fragments arranged by size:
901 561 305
SapI GCTCTTCn'nnn_
Cuts at: 0 593 1767
Size: 593 1174
SfcI C'TryA_G
Cuts at: 0 829 1767
Size: 829 938
SmaI CCC'GGG
Cuts at: 0 325 1767
Size: 325 1442
SmlI C'TyrA_G
Cuts at: 0 1330 1767
Size: 1330 437
SphI G_CATG'C
Cuts at: 0 200 456 1767
Size: 200 256 1311
Fragments arranged by size:
1311 256 200
StyI C'CwwG_G
Cuts at: 0 752 965 1106 1140 1274 1556 1767
Size: 752 213 141 34 134 282 211
Fragments arranged by size:
752 282 213 211 141 134 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 296 1767
Size: 296 1471
TatI w'GTAC_w
Cuts at: 0 1025 1767
Size: 1025 742
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 61 93 1767
Size: 61 32 1674
Fragments arranged by size:
1674 61 32
Tth111I GACn'n_nGTC
Cuts at: 0 91 1767
Size: 91 1676
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 444 600 735 1767
Size: 444 156 135 1032
Fragments arranged by size:
1032 444 156 135
XmaI C'CCGG_G
Cuts at: 0 323 1767
Size: 323 1444
XmnI GAAnn'nnTTC
Cuts at: 0 959 1767
Size: 959 808
ZraI GAC'GTC
Cuts at: 0 141 1767
Size: 141 1626
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AflII AflIII AleI
AlfI AloI AlwNI ApaLI ApoI AvaI BaeI BanI
BciVI BfrBI Bme1580I BmgBI BmrI BpmI BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrFI BsrGI BssSI BstAPI
BstEII BstXI BtgI BtgZI BtsI DrdI EaeI EagI
EarI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HincII
KpnI MmeI MscI MslI MspA1I NcoI NsiI NspI
PflMI PfoI PpiI PpuMI PsiI PstI PvuII SapI
SfcI SmaI SmlI SphI StyI TaqII TatI TstI
Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AclI AgeI AhdI ApaI AscI AseI AsiSI
AvrII BamHI BanII BbeI BbsI BbvCI BcgI BclI
BglI BglII BlpI BmtI BplI Bpu10I BpuEI BsaBI
BsaWI BsiWI BspEI BsrBI BsrDI BssHII BstBI BstYI
BstZ17I Bsu36I ClaI CspCI DraI DraIII EciI EcoICRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PmeI PmlI PshAI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SrfI SspI StuI SwaI XbaI XhoI