Restriction map for
pFN21AB9452
(Linear) (Six-base) MAPSORT of: KIBB9452.seq Check: 6210 from: 1 to: 1557
KIBB9452.orf
With 182 enzymes: SgfI *
March 22, 2012 15:45 ..
AccI GT'mk_AC
Cuts at: 0 1175 1557
Size: 1175 382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 1117 1557
Size: 219 898 440
Fragments arranged by size:
898 440 219
AhdI GACnn_n'nnGTC
Cuts at: 0 1350 1557
Size: 1350 207
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 268 1557
Size: 268 1289
AlwNI CAG_nnn'CTG
Cuts at: 0 489 690 1107 1557
Size: 489 201 417 450
Fragments arranged by size:
489 450 417 201
ApaI G_GGCC'C
Cuts at: 0 1114 1557
Size: 1114 443
ApoI r'AATT_y
Cuts at: 0 364 1287 1557
Size: 364 923 270
Fragments arranged by size:
923 364 270
AvaI C'yCGr_G
Cuts at: 0 87 460 1557
Size: 87 373 1097
Fragments arranged by size:
1097 373 87
BanI G'GyrC_C
Cuts at: 0 632 675 1313 1557
Size: 632 43 638 244
Fragments arranged by size:
638 632 244 43
BanII G_rGCy'C
Cuts at: 0 630 1114 1340 1557
Size: 630 484 226 217
Fragments arranged by size:
630 484 226 217
BbeI G_GCGC'C
Cuts at: 0 636 1557
Size: 636 921
BbsI GAAGACnn'nnnn_
Cuts at: 0 1069 1463 1557
Size: 1069 394 94
Fragments arranged by size:
1069 394 94
BbvCI CC'TCA_GC
Cuts at: 0 52 760 1557
Size: 52 708 797
Fragments arranged by size:
797 708 52
BfrBI ATG'CAT
Cuts at: 0 1089 1557
Size: 1089 468
BlpI GC'TnA_GC
Cuts at: 0 1407 1557
Size: 1407 150
Bme1580I G_kGCm'C
Cuts at: 0 301 680 1114 1557
Size: 301 379 434 443
Fragments arranged by size:
443 434 379 301
BmgBI CAC'GTC
Cuts at: 0 710 1557
Size: 710 847
BmrI ACTGGGnnnn_n'
Cuts at: 0 211 992 1557
Size: 211 781 565
Fragments arranged by size:
781 565 211
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 327 1148 1409 1557
Size: 327 821 261 148
Fragments arranged by size:
821 327 261 148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 712 1557
Size: 199 513 845
Fragments arranged by size:
845 513 199
Bpu10I CC'TnA_GC
Cuts at: 0 52 619 760 1339 1557
Size: 52 567 141 579 218
Fragments arranged by size:
579 567 218 141 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 823 1147 1557
Size: 823 324 410
Fragments arranged by size:
823 410 324
BsaI GGTCTCn'nnnn_
Cuts at: 0 553 1557
Size: 553 1004
BsaAI yAC'GTr
Cuts at: 0 1554 1557
Size: 1554 3
BsaHI Gr'CG_yC
Cuts at: 0 143 530 633 1557
Size: 143 387 103 924
Fragments arranged by size:
924 387 143 103
BsaWI w'CCGG_w
Cuts at: 0 740 1557
Size: 740 817
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 685 729 766 769 1477 1486 1489 1492
Size: 685 44 37 3 708 9 3 3
Cuts at: 1492 1501 1504 1507 1557
Size: 9 3 3 50
Fragments arranged by size:
708 685 50 44 37 9 9 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 27 294 563 1557
Size: 27 267 269 994
Fragments arranged by size:
994 269 267 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 708 1278 1557
Size: 708 570 279
Fragments arranged by size:
708 570 279
BsiEI CG_ry'CG
Cuts at: 0 17 1557
Size: 17 1540
BsiHKAI G_wGCw'C
Cuts at: 0 449 1134 1340 1557
Size: 449 685 206 217
Fragments arranged by size:
685 449 217 206
BsiWI C'GTAC_G
Cuts at: 0 1442 1557
Size: 1442 115
BsmBI CGTCTCn'nnnn_
Cuts at: 0 150 1557
Size: 150 1407
Bsp1286I G_dGCh'C
Cuts at: 0 301 449 630 680 1114 1134 1340 1557
Size: 301 148 181 50 434 20 206 217
Fragments arranged by size:
434 301 217 206 181 148 50 20
BspHI T'CATG_A
Cuts at: 0 1358 1557
Size: 1358 199
BsrBI CCG'CTC
Cuts at: 0 495 1379 1557
Size: 495 884 178
Fragments arranged by size:
884 495 178
BsrDI GCAATG_nn'
Cuts at: 0 1278 1557
Size: 1278 279
BsrFI r'CCGG_y
Cuts at: 0 1008 1557
Size: 1008 549
BstAPI GCAn_nnn'nTGC
Cuts at: 0 74 1557
Size: 74 1483
BstXI CCAn_nnnn'nTGG
Cuts at: 0 177 1557
Size: 177 1380
BstYI r'GATC_y
Cuts at: 0 152 335 1557
Size: 152 183 1222
Fragments arranged by size:
1222 183 152
BtgI C'CryG_G
Cuts at: 0 44 734 1557
Size: 44 690 823
Fragments arranged by size:
823 690 44
BtsI GCAGTG_nn'
Cuts at: 0 1524 1557
Size: 1524 33
DraIII CAC_nnn'GTG
Cuts at: 0 219 1557
Size: 219 1338
DrdI GACnn_nn'nnGTC
Cuts at: 0 1009 1446 1557
Size: 1009 437 111
Fragments arranged by size:
1009 437 111
EaeI y'GGCC_r
Cuts at: 0 14 1557
Size: 14 1543
EagI C'GGCC_G
Cuts at: 0 14 1557
Size: 14 1543
EarI CTCTTCn'nnn_
Cuts at: 0 262 1241 1478 1557
Size: 262 979 237 79
Fragments arranged by size:
979 262 237 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 22 1439 1557
Size: 22 1417 118
Fragments arranged by size:
1417 118 22
EcoICRI GAG'CTC
Cuts at: 0 1338 1557
Size: 1338 219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 219 712 1117 1557
Size: 199 20 493 405 440
Fragments arranged by size:
493 440 405 199 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1099 1557
Size: 1099 458
EcoO109I rG'GnC_Cy
Cuts at: 0 201 880 1104 1193 1557
Size: 201 679 224 89 364
Fragments arranged by size:
679 364 224 201 89
EcoRI G'AATT_C
Cuts at: 0 1287 1557
Size: 1287 270
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 489 1557
Size: 489 1068
HaeII r_GCGC'y
Cuts at: 0 636 1557
Size: 636 921
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1336 1368 1557
Size: 1336 32 189
Fragments arranged by size:
1336 189 32
HincII GTy'rAC
Cuts at: 0 657 1557
Size: 657 900
KasI G'GCGC_C
Cuts at: 0 632 1557
Size: 632 925
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1535 1557
Size: 1535 22
MslI CAynn'nnrTG
Cuts at: 0 1271 1557
Size: 1271 286
MspA1I CmG'CkG
Cuts at: 0 1437 1557
Size: 1437 120
NarI GG'CG_CC
Cuts at: 0 633 1557
Size: 633 924
NsiI A_TGCA'T
Cuts at: 0 1091 1557
Size: 1091 466
PflMI CCAn_nnn'nTGG
Cuts at: 0 393 1557
Size: 393 1164
PfoI T'CCnGG_A
Cuts at: 0 148 1184 1557
Size: 148 1036 373
Fragments arranged by size:
1036 373 148
PpuMI rG'GwC_Cy
Cuts at: 0 201 1557
Size: 201 1356
PshAI GACnn'nnGTC
Cuts at: 0 779 1557
Size: 779 778
PspOMI G'GGCC_C
Cuts at: 0 1110 1557
Size: 1110 447
PstI C_TGCA'G
Cuts at: 0 702 1557
Size: 702 855
PvuII CAG'CTG
Cuts at: 0 1437 1557
Size: 1437 120
SacI G_AGCT'C
Cuts at: 0 1340 1557
Size: 1340 217
ScaI AGT'ACT
Cuts at: 0 391 1557
Size: 391 1166
SfcI C'TryA_G
Cuts at: 0 698 1038 1557
Size: 698 340 519
Fragments arranged by size:
698 519 340
SfoI GGC'GCC
Cuts at: 0 634 1557
Size: 634 923
SmaI CCC'GGG
Cuts at: 0 89 1557
Size: 89 1468
SmlI C'TyrA_G
Cuts at: 0 838 1162 1557
Size: 838 324 395
Fragments arranged by size:
838 395 324
StuI AGG'CCT
Cuts at: 0 472 1389 1557
Size: 472 917 168
Fragments arranged by size:
917 472 168
StyI C'CwwG_G
Cuts at: 0 197 339 1412 1557
Size: 197 142 1073 145
Fragments arranged by size:
1073 197 145 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 230 525 842 1298 1557
Size: 230 295 317 456 259
Fragments arranged by size:
456 317 295 259 230
TatI w'GTAC_w
Cuts at: 0 185 389 1154 1557
Size: 185 204 765 403
Fragments arranged by size:
765 403 204 185
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1113 1145 1297 1329 1557
Size: 1113 32 152 32 228
Fragments arranged by size:
1113 228 152 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1171 1557
Size: 1171 386
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 174 687 1557
Size: 174 513 870
Fragments arranged by size:
870 513 174
XmaI C'CCGG_G
Cuts at: 0 87 1557
Size: 87 1470
Enzymes that do cut:
AccI AcuI AhdI AlfI AlwNI ApaI ApoI AvaI
BanI BanII BbeI BbsI BbvCI BfrBI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BspHI BsrBI BsrDI BsrFI BstAPI BstXI
BstYI BtgI BtsI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I HincII KasI MmeI MslI MspA1I NarI NsiI
PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI
ScaI SfcI SfoI SmaI SmlI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BcgI BciVI BclI BglI BglII BmtI BsaBI
BsaXI BsmI BspEI BspMI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI EcoRV
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NspI PacI PasI PciI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI XmnI ZraI