Restriction map for
pFN21AB9441
(Linear) (Six-base) MAPSORT of: KIBB9441.seq Check: 2753 from: 1 to: 1746
KIBB9441.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AccI GT'mk_AC
Cuts at: 0 1703 1746
Size: 1703 43
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 992 1107 1363 1427 1478 1746
Size: 67 925 115 256 64 51 268
Fragments arranged by size:
925 268 256 115 67 64 51
AleI CACnn'nnGTG
Cuts at: 0 871 1486 1746
Size: 871 615 260
Fragments arranged by size:
871 615 260
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1039 1381 1746
Size: 1039 342 365
Fragments arranged by size:
1039 365 342
AlwNI CAG_nnn'CTG
Cuts at: 0 1081 1716 1746
Size: 1081 635 30
Fragments arranged by size:
1081 635 30
ApaI G_GGCC'C
Cuts at: 0 1131 1746
Size: 1131 615
ApoI r'AATT_y
Cuts at: 0 259 1746
Size: 259 1487
AseI AT'TA_AT
Cuts at: 0 1651 1746
Size: 1651 95
AvaI C'yCGr_G
Cuts at: 0 410 1746
Size: 410 1336
BamHI G'GATC_C
Cuts at: 0 1401 1746
Size: 1401 345
BanI G'GyrC_C
Cuts at: 0 1456 1746
Size: 1456 290
BanII G_rGCy'C
Cuts at: 0 761 804 946 1120 1131 1746
Size: 761 43 142 174 11 615
Fragments arranged by size:
761 615 174 142 43 11
BbeI G_GCGC'C
Cuts at: 0 1460 1746
Size: 1460 286
BbsI GAAGACnn'nnnn_
Cuts at: 0 145 1042 1484 1746
Size: 145 897 442 262
Fragments arranged by size:
897 442 262 145
BbvCI CC'TCA_GC
Cuts at: 0 1344 1746
Size: 1344 402
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 287 321 391 425 1746
Size: 287 34 70 34 1321
Fragments arranged by size:
1321 287 70 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1638 1746
Size: 1638 108
BglII A'GATC_T
Cuts at: 0 1537 1746
Size: 1537 209
BlpI GC'TnA_GC
Cuts at: 0 481 1087 1746
Size: 481 606 659
Fragments arranged by size:
659 606 481
Bme1580I G_kGCm'C
Cuts at: 0 1131 1746
Size: 1131 615
BmtI G_CTAG'C
Cuts at: 0 1285 1746
Size: 1285 461
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1329 1746
Size: 1329 417
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 519 869 1168 1624 1746
Size: 493 26 350 299 456 122
Fragments arranged by size:
493 456 350 299 122 26
Bpu10I CC'TnA_GC
Cuts at: 0 1082 1344 1354 1746
Size: 1082 262 10 392
Fragments arranged by size:
1082 392 262 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1570 1746
Size: 1570 176
BsaI GGTCTCn'nnnn_
Cuts at: 0 1061 1746
Size: 1061 685
BsaHI Gr'CG_yC
Cuts at: 0 1457 1746
Size: 1457 289
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 397 812 1565 1746
Size: 397 415 753 181
Fragments arranged by size:
753 415 397 181
BseYI C'CCAG_C
Cuts at: 0 19 398 427 997 1746
Size: 19 379 29 570 749
Fragments arranged by size:
749 570 379 29 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 968 1438 1746
Size: 968 470 308
Fragments arranged by size:
968 470 308
Bsp1286I G_dGCh'C
Cuts at: 0 761 804 946 1120 1131 1746
Size: 761 43 142 174 11 615
Fragments arranged by size:
761 615 174 142 43 11
BspHI T'CATG_A
Cuts at: 0 1695 1746
Size: 1695 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1701 1746
Size: 1701 45
BsrDI GCAATG_nn'
Cuts at: 0 1253 1746
Size: 1253 493
BsrGI T'GTAC_A
Cuts at: 0 104 1746
Size: 104 1642
BstXI CCAn_nnnn'nTGG
Cuts at: 0 439 1746
Size: 439 1307
BstYI r'GATC_y
Cuts at: 0 1401 1537 1746
Size: 1401 136 209
Fragments arranged by size:
1401 209 136
Bsu36I CC'TnA_GG
Cuts at: 0 1424 1746
Size: 1424 322
BtgI C'CryG_G
Cuts at: 0 192 1746
Size: 192 1554
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 60 1746
Size: 60 1686
BtsI GCAGTG_nn'
Cuts at: 0 1037 1481 1493 1746
Size: 1037 444 12 253
Fragments arranged by size:
1037 444 253 12
EarI CTCTTCn'nnn_
Cuts at: 0 374 653 1746
Size: 374 279 1093
Fragments arranged by size:
1093 374 279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 493 519 869 992 1107 1168 1363
Size: 67 426 26 350 123 115 61 195
Cuts at: 1363 1427 1478 1624 1746
Size: 64 51 146 122
Fragments arranged by size:
426 350 195 146 123 122 115 67
64 61 51 26
EcoO109I rG'GnC_Cy
Cuts at: 0 155 1127 1128 1746
Size: 155 972 1 618
Fragments arranged by size:
972 618 155 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 197 1746
Size: 197 1549
HaeII r_GCGC'y
Cuts at: 0 10 1460 1746
Size: 10 1450 286
Fragments arranged by size:
1450 286 10
HincII GTy'rAC
Cuts at: 0 1199 1746
Size: 1199 547
HindIII A'AGCT_T
Cuts at: 0 300 1746
Size: 300 1446
KasI G'GCGC_C
Cuts at: 0 1456 1746
Size: 1456 290
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 946 1746
Size: 946 800
MslI CAynn'nnrTG
Cuts at: 0 871 1021 1486 1746
Size: 871 150 465 260
Fragments arranged by size:
871 465 260 150
MspA1I CmG'CkG
Cuts at: 0 970 1153 1273 1746
Size: 970 183 120 473
Fragments arranged by size:
970 473 183 120
NarI GG'CG_CC
Cuts at: 0 1457 1746
Size: 1457 289
NcoI C'CATG_G
Cuts at: 0 192 1746
Size: 192 1554
NheI G'CTAG_C
Cuts at: 0 1281 1746
Size: 1281 465
NspI r_CATG'y
Cuts at: 0 919 1746
Size: 919 827
PasI CC'CwG_GG
Cuts at: 0 541 819 1746
Size: 541 278 927
Fragments arranged by size:
927 541 278
PflMI CCAn_nnn'nTGG
Cuts at: 0 131 1746
Size: 131 1615
PfoI T'CCnGG_A
Cuts at: 0 470 1640 1746
Size: 470 1170 106
Fragments arranged by size:
1170 470 106
PpuMI rG'GwC_Cy
Cuts at: 0 155 1746
Size: 155 1591
PshAI GACnn'nnGTC
Cuts at: 0 1053 1746
Size: 1053 693
PspOMI G'GGCC_C
Cuts at: 0 1127 1746
Size: 1127 619
PstI C_TGCA'G
Cuts at: 0 448 1488 1746
Size: 448 1040 258
Fragments arranged by size:
1040 448 258
PvuII CAG'CTG
Cuts at: 0 970 1153 1273 1746
Size: 970 183 120 473
Fragments arranged by size:
970 473 183 120
SfcI C'TryA_G
Cuts at: 0 444 1484 1746
Size: 444 1040 262
Fragments arranged by size:
1040 444 262
SfoI GGC'GCC
Cuts at: 0 1458 1746
Size: 1458 288
SmlI C'TyrA_G
Cuts at: 0 1549 1746
Size: 1549 197
SphI G_CATG'C
Cuts at: 0 919 1746
Size: 919 827
StyI C'CwwG_G
Cuts at: 0 119 192 491 1746
Size: 119 73 299 1255
Fragments arranged by size:
1255 299 119 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 508 811 1746
Size: 508 303 935
Fragments arranged by size:
935 508 303
TatI w'GTAC_w
Cuts at: 0 104 1687 1746
Size: 104 1583 59
Fragments arranged by size:
1583 104 59
XbaI T'CTAG_A
Cuts at: 0 591 1746
Size: 591 1155
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 468 1746
Size: 468 1278
XmnI GAAnn'nnTTC
Cuts at: 0 278 1611 1746
Size: 278 1333 135
Fragments arranged by size:
1333 278 135
Enzymes that do cut:
AccI AcuI AleI AlfI AlwNI ApaI ApoI AseI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BglII BlpI Bme1580I BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BseRI BseYI BsgI Bsp1286I BspHI
BspMI BsrDI BsrGI BstXI BstYI Bsu36I BtgI BtgZI
BtsI EarI Eco57MI EcoO109I FalI HaeII HincII HindIII
KasI MmeI MslI MspA1I NarI NcoI NheI NspI
PasI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII
SfcI SfoI SmlI SphI StyI TaqII TatI XbaI
XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaLI AscI AsiSI AvrII BaeI BclI
BfrBI BglI BmgBI BmrI BsaAI BsaBI BsaWI BsaXI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstZ17I ClaI CspCI
DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FseI FspI FspAI Hin4I HpaI KpnI
MfeI MluI MscI NaeI NdeI NgoMIV NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI TstI Tth111I XhoI
XmaI ZraI