Restriction map for
pFN21AB9439
(Linear) (Six-base) MAPSORT of: KIBB9439.seq Check: 7776 from: 1 to: 1389
KIBB9439.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 375 1389
Size: 375 1014
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 642 1389
Size: 215 427 747
Fragments arranged by size:
747 427 215
AflIII A'CryG_T
Cuts at: 0 710 1389
Size: 710 679
AlwNI CAG_nnn'CTG
Cuts at: 0 333 378 1389
Size: 333 45 1011
Fragments arranged by size:
1011 333 45
ApaI G_GGCC'C
Cuts at: 0 1354 1389
Size: 1354 35
AvaI C'yCGr_G
Cuts at: 0 479 532 1389
Size: 479 53 857
Fragments arranged by size:
857 479 53
BanI G'GyrC_C
Cuts at: 0 995 1389
Size: 995 394
BanII G_rGCy'C
Cuts at: 0 24 462 542 1354 1389
Size: 24 438 80 812 35
Fragments arranged by size:
812 438 80 35 24
BbeI G_GCGC'C
Cuts at: 0 999 1389
Size: 999 390
BbsI GAAGACnn'nnnn_
Cuts at: 0 203 1389
Size: 203 1186
BbvCI CC'TCA_GC
Cuts at: 0 239 443 1164 1389
Size: 239 204 721 225
Fragments arranged by size:
721 239 225 204
BglI GCCn_nnn'nGGC
Cuts at: 0 407 833 1304 1389
Size: 407 426 471 85
Fragments arranged by size:
471 426 407 85
BlpI GC'TnA_GC
Cuts at: 0 799 1389
Size: 799 590
Bme1580I G_kGCm'C
Cuts at: 0 1354 1389
Size: 1354 35
BmrI ACTGGGnnnn_n'
Cuts at: 0 170 197 536 1389
Size: 170 27 339 853
Fragments arranged by size:
853 339 170 27
BmtI G_CTAG'C
Cuts at: 0 298 1389
Size: 298 1091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 70 304 310 343 571 580 754
Size: 8 62 234 6 33 228 9 174
Cuts at: 754 1220 1389
Size: 466 169
Fragments arranged by size:
466 234 228 174 169 62 33 9
8 6
Bpu10I CC'TnA_GC
Cuts at: 0 239 443 697 1164 1389
Size: 239 204 254 467 225
Fragments arranged by size:
467 254 239 225 204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 759 1286 1389
Size: 706 53 527 103
Fragments arranged by size:
706 527 103 53
BsaHI Gr'CG_yC
Cuts at: 0 996 1389
Size: 996 393
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 299 329 1389
Size: 299 30 1060
Fragments arranged by size:
1060 299 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 307 550 568 940 1389
Size: 307 243 18 372 449
Fragments arranged by size:
449 372 307 243 18
BseYI C'CCAG_C
Cuts at: 0 219 248 298 392 473 600 784 808
Size: 219 29 50 94 81 127 184 24
Cuts at: 808 1045 1186 1294 1342 1389
Size: 237 141 108 48 47
Fragments arranged by size:
237 219 184 141 127 108 94 81
50 48 47 29 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 937 1389
Size: 937 452
BsiEI CG_ry'CG
Cuts at: 0 837 1389
Size: 837 552
BsiHKAI G_wGCw'C
Cuts at: 0 89 462 1144 1389
Size: 89 373 682 245
Fragments arranged by size:
682 373 245 89
BsmBI CGTCTCn'nnnn_
Cuts at: 0 834 1389
Size: 834 555
Bsp1286I G_dGCh'C
Cuts at: 0 24 89 462 542 1144 1354 1389
Size: 24 65 373 80 602 210 35
Fragments arranged by size:
602 373 210 80 65 35 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 375 1389
Size: 375 1014
BsrFI r'CCGG_y
Cuts at: 0 243 992 1059 1389
Size: 243 749 67 330
Fragments arranged by size:
749 330 243 67
BstAPI GCAn_nnn'nTGC
Cuts at: 0 336 1389
Size: 336 1053
BstXI CCAn_nnnn'nTGG
Cuts at: 0 298 513 549 1389
Size: 298 215 36 840
Fragments arranged by size:
840 298 215 36
BstYI r'GATC_y
Cuts at: 0 929 1389
Size: 929 460
BtgI C'CryG_G
Cuts at: 0 632 1389
Size: 632 757
BtsI GCAGTG_nn'
Cuts at: 0 1032 1389
Size: 1032 357
EaeI y'GGCC_r
Cuts at: 0 629 834 1061 1271 1389
Size: 629 205 227 210 118
Fragments arranged by size:
629 227 210 205 118
EagI C'GGCC_G
Cuts at: 0 834 1389
Size: 834 555
EarI CTCTTCn'nnn_
Cuts at: 0 467 844 886 1389
Size: 467 377 42 503
Fragments arranged by size:
503 467 377 42
EcoICRI GAG'CTC
Cuts at: 0 460 1389
Size: 460 929
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 70 215 304 310 343 571 580
Size: 8 62 145 89 6 33 228 9
Cuts at: 580 642 754 1220 1389
Size: 62 112 466 169
Fragments arranged by size:
466 228 169 145 112 89 62 62
33 9 8 6
EcoO109I rG'GnC_Cy
Cuts at: 0 687 1106 1351 1389
Size: 687 419 245 38
Fragments arranged by size:
687 419 245 38
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 621 1389
Size: 621 768
HaeII r_GCGC'y
Cuts at: 0 999 1389
Size: 999 390
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 64 96 411 443 508 540 748 780
Size: 64 32 315 32 65 32 208 32
Cuts at: 780 1389
Size: 609
Fragments arranged by size:
609 315 208 65 64 32 32 32
32
HincII GTy'rAC
Cuts at: 0 281 1389
Size: 281 1108
HindIII A'AGCT_T
Cuts at: 0 1124 1389
Size: 1124 265
KasI G'GCGC_C
Cuts at: 0 995 1389
Size: 995 394
MscI TGG'CCA
Cuts at: 0 631 1389
Size: 631 758
MspA1I CmG'CkG
Cuts at: 0 333 798 951 1168 1198 1389
Size: 333 465 153 217 30 191
Fragments arranged by size:
465 333 217 191 153 30
NaeI GCC'GGC
Cuts at: 0 245 1061 1389
Size: 245 816 328
Fragments arranged by size:
816 328 245
NarI GG'CG_CC
Cuts at: 0 996 1389
Size: 996 393
NcoI C'CATG_G
Cuts at: 0 632 1389
Size: 632 757
NdeI CA'TA_TG
Cuts at: 0 1079 1389
Size: 1079 310
NgoMIV G'CCGG_C
Cuts at: 0 243 1059 1389
Size: 243 816 330
Fragments arranged by size:
816 330 243
NheI G'CTAG_C
Cuts at: 0 294 1389
Size: 294 1095
NspI r_CATG'y
Cuts at: 0 714 1389
Size: 714 675
PasI CC'CwG_GG
Cuts at: 0 453 1051 1389
Size: 453 598 338
Fragments arranged by size:
598 453 338
PciI A'CATG_T
Cuts at: 0 710 1389
Size: 710 679
PflMI CCAn_nnn'nTGG
Cuts at: 0 333 1389
Size: 333 1056
PfoI T'CCnGG_A
Cuts at: 0 731 1389
Size: 731 658
PpuMI rG'GwC_Cy
Cuts at: 0 1106 1389
Size: 1106 283
PshAI GACnn'nnGTC
Cuts at: 0 871 1389
Size: 871 518
PspOMI G'GGCC_C
Cuts at: 0 1350 1389
Size: 1350 39
PstI C_TGCA'G
Cuts at: 0 1027 1389
Size: 1027 362
PvuII CAG'CTG
Cuts at: 0 333 798 951 1168 1198 1389
Size: 333 465 153 217 30 191
Fragments arranged by size:
465 333 217 191 153 30
SacI G_AGCT'C
Cuts at: 0 462 1389
Size: 462 927
SapI GCTCTTCn'nnn_
Cuts at: 0 467 1389
Size: 467 922
SfcI C'TryA_G
Cuts at: 0 1023 1068 1389
Size: 1023 45 321
Fragments arranged by size:
1023 321 45
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 833 1389
Size: 833 556
SfoI GGC'GCC
Cuts at: 0 997 1389
Size: 997 392
SmaI CCC'GGG
Cuts at: 0 481 1389
Size: 481 908
SmlI C'TyrA_G
Cuts at: 0 721 774 1301 1389
Size: 721 53 527 88
Fragments arranged by size:
721 527 88 53
StuI AGG'CCT
Cuts at: 0 410 1334 1389
Size: 410 924 55
Fragments arranged by size:
924 410 55
StyI C'CwwG_G
Cuts at: 0 632 1092 1389
Size: 632 460 297
Fragments arranged by size:
632 460 297
TatI w'GTAC_w
Cuts at: 0 178 1389
Size: 178 1211
Tth111I GACn'n_nGTC
Cuts at: 0 762 1389
Size: 762 627
XmaI C'CCGG_G
Cuts at: 0 479 1389
Size: 479 910
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApaI AvaI BanI BanII
BbeI BbsI BbvCI BglI BlpI Bme1580I BmrI BmtI
BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BstAPI BstXI
BstYI BtgI BtsI EaeI EagI EarI EcoICRI Eco57MI
EcoO109I FalI HaeII Hin4I HincII HindIII KasI MscI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI
PasI PciI PflMI PfoI PpuMI PshAI PspOMI PstI
PvuII SacI SapI SfcI SfiI SfoI SmaI SmlI
StuI StyI TatI Tth111I XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglII
BmgBI BplI BsaI BsaAI BsaBI BsaWI BsiWI BsmI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EciI EcoNI EcoRI EcoRV FseI FspI FspAI
HpaI KpnI MfeI MluI MmeI MslI NotI NruI
NsiI PacI PmeI PmlI PpiI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TstI XbaI XcmI XhoI XmnI ZraI