Restriction map for
pFN21AB9437
(Linear) (Six-base) MAPSORT of: KIBB9437.seq Check: 2643 from: 1 to: 1785
KIBB9437.orf
With 182 enzymes: SgfI *
April 28, 2014 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 620 1785
Size: 620 1165
AccI GT'mk_AC
Cuts at: 0 560 1785
Size: 560 1225
AfeI AGC'GCT
Cuts at: 0 37 1785
Size: 37 1748
AleI CACnn'nnGTG
Cuts at: 0 577 1785
Size: 577 1208
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1165 1197 1785
Size: 1165 32 588
Fragments arranged by size:
1165 588 32
AlwNI CAG_nnn'CTG
Cuts at: 0 176 1785
Size: 176 1609
ApaI G_GGCC'C
Cuts at: 0 160 304 1785
Size: 160 144 1481
Fragments arranged by size:
1481 160 144
ApaLI G'TGCA_C
Cuts at: 0 508 1785
Size: 508 1277
ApoI r'AATT_y
Cuts at: 0 1460 1785
Size: 1460 325
AscI GG'CGCG_CC
Cuts at: 0 491 1785
Size: 491 1294
AvaI C'yCGr_G
Cuts at: 0 140 304 421 1118 1663 1785
Size: 140 164 117 697 545 122
Fragments arranged by size:
697 545 164 140 122 117
BanI G'GyrC_C
Cuts at: 0 166 285 397 820 1026 1113 1213 1428
Size: 166 119 112 423 206 87 100 215
Cuts at: 1428 1624 1785
Size: 196 161
Fragments arranged by size:
423 215 206 196 166 161 119 112
100 87
BanII G_rGCy'C
Cuts at: 0 95 160 270 304 412 923 1006 1104
Size: 95 65 110 34 108 511 83 98
Cuts at: 1104 1247 1679 1703 1785
Size: 143 432 24 82
Fragments arranged by size:
511 432 143 110 108 98 95 83
82 65 34 24
BbeI G_GCGC'C
Cuts at: 0 170 289 401 824 1030 1785
Size: 170 119 112 423 206 755
Fragments arranged by size:
755 423 206 170 119 112
BbvCI CC'TCA_GC
Cuts at: 0 188 1267 1785
Size: 188 1079 518
Fragments arranged by size:
1079 518 188
BglI GCCn_nnn'nGGC
Cuts at: 0 164 308 526 541 1044 1200 1618 1729
Size: 164 144 218 15 503 156 418 111
Cuts at: 1729 1785
Size: 56
Fragments arranged by size:
503 418 218 164 156 144 111 56
15
BlpI GC'TnA_GC
Cuts at: 0 406 759 1785
Size: 406 353 1026
Fragments arranged by size:
1026 406 353
Bme1580I G_kGCm'C
Cuts at: 0 160 304 512 569 1118 1433 1629 1785
Size: 160 144 208 57 549 315 196 156
Fragments arranged by size:
549 315 208 196 160 156 144 57
BmgBI CAC'GTC
Cuts at: 0 774 1742 1785
Size: 774 968 43
Fragments arranged by size:
968 774 43
BmrI ACTGGGnnnn_n'
Cuts at: 0 918 1121 1785
Size: 918 203 664
Fragments arranged by size:
918 664 203
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1123 1785
Size: 1123 662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 447 1162 1785
Size: 54 393 715 623
Fragments arranged by size:
715 623 393 54
Bpu10I CC'TnA_GC
Cuts at: 0 188 1267 1785
Size: 188 1079 518
Fragments arranged by size:
1079 518 188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1663 1785
Size: 1663 122
BsaI GGTCTCn'nnnn_
Cuts at: 0 1057 1785
Size: 1057 728
BsaAI yAC'GTr
Cuts at: 0 564 1020 1785
Size: 564 456 765
Fragments arranged by size:
765 564 456
BsaHI Gr'CG_yC
Cuts at: 0 59 167 184 286 398 821 1027 1036
Size: 59 108 17 102 112 423 206 9
Cuts at: 1036 1192 1712 1785
Size: 156 520 73
Fragments arranged by size:
520 423 206 156 112 108 102 73
59 17 9
BsaWI w'CCGG_w
Cuts at: 0 1755 1785
Size: 1755 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 424 454 1135 1165 1195 1785
Size: 424 30 681 30 30 590
Fragments arranged by size:
681 590 424 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 120 448 1785
Size: 120 328 1337
Fragments arranged by size:
1337 328 120
BseYI C'CCAG_C
Cuts at: 0 323 799 1785
Size: 323 476 986
Fragments arranged by size:
986 476 323
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1060 1151 1785
Size: 1060 91 634
Fragments arranged by size:
1060 634 91
BsiEI CG_ry'CG
Cuts at: 0 212 368 479 521 689 915 944 952
Size: 212 156 111 42 168 226 29 8
Cuts at: 952 1733 1756 1785
Size: 781 23 29
Fragments arranged by size:
781 226 212 168 156 111 42 29
29 23 8
BsiHKAI G_wGCw'C
Cuts at: 0 512 1657 1679 1785
Size: 512 1145 22 106
Fragments arranged by size:
1145 512 106 22
BsmBI CGTCTCn'nnnn_
Cuts at: 0 780 1785
Size: 780 1005
Bsp1286I G_dGCh'C
Cuts at: 0 95 160 270 304 412 512 569 923
Size: 95 65 110 34 108 100 57 354
Cuts at: 923 1006 1104 1118 1247 1433 1629 1657 1679
Size: 83 98 14 129 186 196 28 22
Cuts at: 1679 1703 1785
Size: 24 82
Fragments arranged by size:
354 196 186 129 110 108 100 98
95 83 82 65 57 34 28 24
22 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 620 1145 1785
Size: 620 525 640
Fragments arranged by size:
640 620 525
BsrBI CCG'CTC
Cuts at: 0 153 227 457 881 1107 1785
Size: 153 74 230 424 226 678
Fragments arranged by size:
678 424 230 226 153 74
BsrDI GCAATG_nn'
Cuts at: 0 1401 1785
Size: 1401 384
BsrFI r'CCGG_y
Cuts at: 0 1216 1271 1728 1785
Size: 1216 55 457 57
Fragments arranged by size:
1216 457 57 55
BssHII G'CGCG_C
Cuts at: 0 491 659 781 1785
Size: 491 168 122 1004
Fragments arranged by size:
1004 491 168 122
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1083 1238 1785
Size: 1083 155 547
Fragments arranged by size:
1083 547 155
BstXI CCAn_nnnn'nTGG
Cuts at: 0 579 1785
Size: 579 1206
Bsu36I CC'TnA_GG
Cuts at: 0 677 1614 1785
Size: 677 937 171
Fragments arranged by size:
937 677 171
BtgI C'CryG_G
Cuts at: 0 47 273 393 521 536 737 764 809
Size: 47 226 120 128 15 201 27 45
Cuts at: 809 905 1422 1785
Size: 96 517 363
Fragments arranged by size:
517 363 226 201 128 120 96 47
45 27 15
BtsI GCAGTG_nn'
Cuts at: 0 633 864 1785
Size: 633 231 921
Fragments arranged by size:
921 633 231
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1383 1418 1785
Size: 1383 35 367
Fragments arranged by size:
1383 367 35
EaeI y'GGCC_r
Cuts at: 0 209 476 518 545 686 812 902 912
Size: 209 267 42 27 141 126 90 10
Cuts at: 912 941 949 1218 1730 1785
Size: 29 8 269 512 55
Fragments arranged by size:
512 269 267 209 141 126 90 55
42 29 27 10 8
EagI C'GGCC_G
Cuts at: 0 209 476 518 686 912 941 949 1730
Size: 209 267 42 168 226 29 8 781
Cuts at: 1730 1785
Size: 55
Fragments arranged by size:
781 267 226 209 168 55 42 29
8
EarI CTCTTCn'nnn_
Cuts at: 0 123 1563 1785
Size: 123 1440 222
Fragments arranged by size:
1440 222 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 161 1785
Size: 161 1624
EcoICRI GAG'CTC
Cuts at: 0 1677 1785
Size: 1677 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 447 1162 1785
Size: 54 393 715 623
Fragments arranged by size:
715 623 393 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1141 1785
Size: 1141 644
EcoO109I rG'GnC_Cy
Cuts at: 0 156 157 301 742 1123 1146 1785
Size: 156 1 144 441 381 23 639
Fragments arranged by size:
639 441 381 156 144 23 1
EcoRI G'AATT_C
Cuts at: 0 1460 1785
Size: 1460 325
HaeII r_GCGC'y
Cuts at: 0 39 170 289 401 755 824 1030 1785
Size: 39 131 119 112 354 69 206 755
Fragments arranged by size:
755 354 206 131 119 112 69 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1135 1167 1644 1676 1785
Size: 1135 32 477 32 109
Fragments arranged by size:
1135 477 109 32 32
KasI G'GCGC_C
Cuts at: 0 166 285 397 820 1026 1785
Size: 166 119 112 423 206 759
Fragments arranged by size:
759 423 206 166 119 112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1570 1785
Size: 1570 215
MscI TGG'CCA
Cuts at: 0 814 1785
Size: 814 971
MslI CAynn'nnrTG
Cuts at: 0 577 1537 1785
Size: 577 960 248
Fragments arranged by size:
960 577 248
MspA1I CmG'CkG
Cuts at: 0 49 395 523 538 710 739 766 954
Size: 49 346 128 15 172 29 27 188
Cuts at: 954 1197 1785
Size: 243 588
Fragments arranged by size:
588 346 243 188 172 128 49 29
27 15
NaeI GCC'GGC
Cuts at: 0 1273 1730 1785
Size: 1273 457 55
Fragments arranged by size:
1273 457 55
NarI GG'CG_CC
Cuts at: 0 167 286 398 821 1027 1785
Size: 167 119 112 423 206 758
Fragments arranged by size:
758 423 206 167 119 112
NcoI C'CATG_G
Cuts at: 0 809 905 1422 1785
Size: 809 96 517 363
Fragments arranged by size:
809 517 363 96
NgoMIV G'CCGG_C
Cuts at: 0 1271 1728 1785
Size: 1271 457 57
Fragments arranged by size:
1271 457 57
NotI GC'GGCC_GC
Cuts at: 0 518 912 949 1785
Size: 518 394 37 836
Fragments arranged by size:
836 518 394 37
PflMI CCAn_nnn'nTGG
Cuts at: 0 1361 1785
Size: 1361 424
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1165 1197 1785
Size: 1165 32 588
Fragments arranged by size:
1165 588 32
PpuMI rG'GwC_Cy
Cuts at: 0 1123 1146 1785
Size: 1123 23 639
Fragments arranged by size:
1123 639 23
PspOMI G'GGCC_C
Cuts at: 0 156 300 1785
Size: 156 144 1485
Fragments arranged by size:
1485 156 144
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 949 981 1785
Size: 949 32 804
Fragments arranged by size:
949 804 32
PstI C_TGCA'G
Cuts at: 0 617 1088 1785
Size: 617 471 697
Fragments arranged by size:
697 617 471
SacI G_AGCT'C
Cuts at: 0 1679 1785
Size: 1679 106
SacII CC_GC'GG
Cuts at: 0 50 396 524 539 740 767 1785
Size: 50 346 128 15 201 27 1018
Fragments arranged by size:
1018 346 201 128 50 27 15
SanDI GG'GwC_CC
Cuts at: 0 1123 1785
Size: 1123 662
SbfI CC_TGCA'GG
Cuts at: 0 617 1785
Size: 617 1168
SfcI C'TryA_G
Cuts at: 0 613 1084 1248 1607 1785
Size: 613 471 164 359 178
Fragments arranged by size:
613 471 359 178 164
SfoI GGC'GCC
Cuts at: 0 168 287 399 822 1028 1785
Size: 168 119 112 423 206 757
Fragments arranged by size:
757 423 206 168 119 112
SmaI CCC'GGG
Cuts at: 0 142 306 1120 1785
Size: 142 164 814 665
Fragments arranged by size:
814 665 164 142
SmlI C'TyrA_G
Cuts at: 0 1678 1785
Size: 1678 107
StuI AGG'CCT
Cuts at: 0 682 1785
Size: 682 1103
StyI C'CwwG_G
Cuts at: 0 809 905 1422 1785
Size: 809 96 517 363
Fragments arranged by size:
809 517 363 96
TatI w'GTAC_w
Cuts at: 0 926 1785
Size: 926 859
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1750 1782 1785
Size: 1750 32 3
Fragments arranged by size:
1750 32 3
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 576 807 1785
Size: 576 231 978
Fragments arranged by size:
978 576 231
XmaI C'CCGG_G
Cuts at: 0 140 304 1118 1785
Size: 140 164 814 667
Fragments arranged by size:
814 667 164 140
XmnI GAAnn'nnTTC
Cuts at: 0 37 1785
Size: 37 1748
Enzymes that do cut:
AarI AccI AfeI AleI AloI AlwNI ApaI ApaLI
ApoI AscI AvaI BanI BanII BbeI BbvCI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssHII BstAPI BstXI Bsu36I BtgI BtsI CspCI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI PflMI PpiI PpuMI PspOMI
PsrI PstI SacI SacII SanDI SbfI SfcI SfoI
SmaI SmlI StuI StyI TatI TstI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AflII AflIII AgeI AhdI
AlfI AseI AsiSI AvrII BaeI BamHI BbsI BcgI
BciVI BclI BfrBI BglII BmtI BsaBI BsiWI BsmI
BspEI BspHI BsrGI BssSI BstBI BstEII BstYI BstZ17I
BtgZI ClaI DraI DraIII DrdI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
NdeI NheI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PshAI PsiI PspXI PvuI PvuII
RsrII SalI SapI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I
XbaI XhoI ZraI