Restriction map for
pFN21AB9428
(Linear) (Six-base) MAPSORT of: KIBB9428.seq Check: 9439 from: 1 to: 1554
KIBB9428.orf
With 182 enzymes: SgfI *
July 3, 2012 16:37 ..
AatII G_ACGT'C
Cuts at: 0 611 1554
Size: 611 943
AccI GT'mk_AC
Cuts at: 0 611 1554
Size: 611 943
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 1148 1529 1554
Size: 793 355 381 25
Fragments arranged by size:
793 381 355 25
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1236 1268 1554
Size: 1236 32 286
Fragments arranged by size:
1236 286 32
ApoI r'AATT_y
Cuts at: 0 208 283 1554
Size: 208 75 1271
Fragments arranged by size:
1271 208 75
AvaI C'yCGr_G
Cuts at: 0 1095 1262 1554
Size: 1095 167 292
Fragments arranged by size:
1095 292 167
BamHI G'GATC_C
Cuts at: 0 623 1554
Size: 623 931
BanI G'GyrC_C
Cuts at: 0 28 541 1437 1554
Size: 28 513 896 117
Fragments arranged by size:
896 513 117 28
BanII G_rGCy'C
Cuts at: 0 77 809 1554
Size: 77 732 745
Fragments arranged by size:
745 732 77
BbsI GAAGACnn'nnnn_
Cuts at: 0 849 1554
Size: 849 705
BlpI GC'TnA_GC
Cuts at: 0 728 1554
Size: 728 826
Bme1580I G_kGCm'C
Cuts at: 0 33 331 544 1122 1554
Size: 33 298 213 578 432
Fragments arranged by size:
578 432 298 213 33
BmgBI CAC'GTC
Cuts at: 0 465 1554
Size: 465 1089
BmrI ACTGGGnnnn_n'
Cuts at: 0 258 318 1554
Size: 258 60 1236
Fragments arranged by size:
1236 258 60
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 315 1554
Size: 315 1239
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 816 1178 1536 1554
Size: 82 734 362 358 18
Fragments arranged by size:
734 362 358 82 18
Bpu10I CC'TnA_GC
Cuts at: 0 922 1554
Size: 922 632
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 1554
Size: 412 1142
BsaI GGTCTCn'nnnn_
Cuts at: 0 1192 1554
Size: 1192 362
BsaHI Gr'CG_yC
Cuts at: 0 608 1554
Size: 608 946
BsaWI w'CCGG_w
Cuts at: 0 584 1554
Size: 584 970
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 805 835 1554
Size: 805 30 719
Fragments arranged by size:
805 719 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1078 1125 1141 1554
Size: 1078 47 16 413
Fragments arranged by size:
1078 413 47 16
BseYI C'CCAG_C
Cuts at: 0 22 1072 1225 1554
Size: 22 1050 153 329
Fragments arranged by size:
1050 329 153 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 400 445 1554
Size: 376 24 45 1109
Fragments arranged by size:
1109 376 45 24
BsiEI CG_ry'CG
Cuts at: 0 1342 1554
Size: 1342 212
BsiHKAI G_wGCw'C
Cuts at: 0 77 413 1554
Size: 77 336 1141
Fragments arranged by size:
1141 336 77
BsmI GAATG_Cn'
Cuts at: 0 838 888 1554
Size: 838 50 666
Fragments arranged by size:
838 666 50
Bsp1286I G_dGCh'C
Cuts at: 0 33 77 331 413 544 809 1122 1554
Size: 33 44 254 82 131 265 313 432
Fragments arranged by size:
432 313 265 254 131 82 44 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 398 1122 1554
Size: 398 724 432
Fragments arranged by size:
724 432 398
BsrBI CCG'CTC
Cuts at: 0 345 1344 1554
Size: 345 999 210
Fragments arranged by size:
999 345 210
BsrFI r'CCGG_y
Cuts at: 0 267 713 1554
Size: 267 446 841
Fragments arranged by size:
841 446 267
BssSI C'ACGA_G
Cuts at: 0 682 1554
Size: 682 872
BstAPI GCAn_nnn'nTGC
Cuts at: 0 643 1554
Size: 643 911
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1145 1532 1554
Size: 1145 387 22
Fragments arranged by size:
1145 387 22
BstYI r'GATC_y
Cuts at: 0 258 623 1503 1554
Size: 258 365 880 51
Fragments arranged by size:
880 365 258 51
BtgI C'CryG_G
Cuts at: 0 188 896 1554
Size: 188 708 658
Fragments arranged by size:
708 658 188
BtsI GCAGTG_nn'
Cuts at: 0 644 701 807 1554
Size: 644 57 106 747
Fragments arranged by size:
747 644 106 57
EaeI y'GGCC_r
Cuts at: 0 340 1554
Size: 340 1214
EarI CTCTTCn'nnn_
Cuts at: 0 645 1554
Size: 645 909
EcoICRI GAG'CTC
Cuts at: 0 75 1554
Size: 75 1479
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 793 816 1148 1178 1529 1536 1554
Size: 82 711 23 332 30 351 7 18
Fragments arranged by size:
711 351 332 82 30 23 18 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1518 1554
Size: 1518 36
EcoO109I rG'GnC_Cy
Cuts at: 0 423 1310 1495 1554
Size: 423 887 185 59
Fragments arranged by size:
887 423 185 59
EcoRI G'AATT_C
Cuts at: 0 208 1554
Size: 208 1346
EcoRV GAT'ATC
Cuts at: 0 170 1554
Size: 170 1384
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 391 1554
Size: 391 1163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 805 837 898 930 932 964 1554
Size: 805 32 61 32 2 32 590
Fragments arranged by size:
805 590 61 32 32 32 2
HincII GTy'rAC
Cuts at: 0 576 1350 1554
Size: 576 774 204
Fragments arranged by size:
774 576 204
HpaI GTT'AAC
Cuts at: 0 576 1350 1554
Size: 576 774 204
Fragments arranged by size:
774 576 204
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 1359 1554
Size: 461 898 195
Fragments arranged by size:
898 461 195
MspA1I CmG'CkG
Cuts at: 0 26 1554
Size: 26 1528
NaeI GCC'GGC
Cuts at: 0 269 1554
Size: 269 1285
NcoI C'CATG_G
Cuts at: 0 188 1554
Size: 188 1366
NgoMIV G'CCGG_C
Cuts at: 0 267 1554
Size: 267 1287
PasI CC'CwG_GG
Cuts at: 0 546 1554
Size: 546 1008
PflMI CCAn_nnn'nTGG
Cuts at: 0 867 1255 1554
Size: 867 388 299
Fragments arranged by size:
867 388 299
PfoI T'CCnGG_A
Cuts at: 0 827 1254 1554
Size: 827 427 300
Fragments arranged by size:
827 427 300
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 665 697 1554
Size: 665 32 857
Fragments arranged by size:
857 665 32
PpuMI rG'GwC_Cy
Cuts at: 0 423 1310 1554
Size: 423 887 244
Fragments arranged by size:
887 423 244
PstI C_TGCA'G
Cuts at: 0 320 1309 1554
Size: 320 989 245
Fragments arranged by size:
989 320 245
SacI G_AGCT'C
Cuts at: 0 77 1554
Size: 77 1477
SbfI CC_TGCA'GG
Cuts at: 0 1309 1554
Size: 1309 245
SfcI C'TryA_G
Cuts at: 0 316 612 1305 1554
Size: 316 296 693 249
Fragments arranged by size:
693 316 296 249
SmlI C'TyrA_G
Cuts at: 0 427 1554
Size: 427 1127
StuI AGG'CCT
Cuts at: 0 592 1554
Size: 592 962
StyI C'CwwG_G
Cuts at: 0 41 188 809 963 1554
Size: 41 147 621 154 591
Fragments arranged by size:
621 591 154 147 41
Tth111I GACn'n_nGTC
Cuts at: 0 522 1554
Size: 522 1032
XbaI T'CTAG_A
Cuts at: 0 1059 1554
Size: 1059 495
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 57 195 270 444 1431 1554
Size: 57 138 75 174 987 123
Fragments arranged by size:
987 174 138 123 75 57
ZraI GAC'GTC
Cuts at: 0 609 1554
Size: 609 945
Enzymes that do cut:
AatII AccI AcuI AloI ApoI AvaI BamHI BanI
BanII BbsI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI
BssSI BstAPI BstXI BstYI BtgI BtsI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I
HincII HpaI MmeI MspA1I NaeI NcoI NgoMIV PasI
PflMI PfoI PpiI PpuMI PstI SacI SbfI SfcI
SmlI StuI StyI Tth111I XbaI XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BmtI BsaAI BsaBI BsiWI BsmBI BspEI
BspHI BsrDI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
FseI FspI FspAI HaeII HindIII KasI KpnI MfeI
MluI MscI MslI NarI NdeI NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI
SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII
TatI TstI XhoI XmaI XmnI