Restriction map for
pFN21AB9420
(Linear) (Six-base) MAPSORT of: KIBB9420.seq Check: 6049 from: 1 to: 1569
KIBB9420.orf
With 182 enzymes: SgfI *
December 27, 2011 16:30 ..
AccI GT'mk_AC
Cuts at: 0 393 1569
Size: 393 1176
Acc65I G'GTAC_C
Cuts at: 0 1058 1569
Size: 1058 511
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1345 1352 1412 1569
Size: 1345 7 60 157
Fragments arranged by size:
1345 157 60 7
AflIII A'CryG_T
Cuts at: 0 1112 1407 1569
Size: 1112 295 162
Fragments arranged by size:
1112 295 162
ApaLI G'TGCA_C
Cuts at: 0 689 1569
Size: 689 880
ApoI r'AATT_y
Cuts at: 0 7 645 887 1354 1512 1569
Size: 7 638 242 467 158 57
Fragments arranged by size:
638 467 242 158 57 7
AseI AT'TA_AT
Cuts at: 0 962 1569
Size: 962 607
AvaI C'yCGr_G
Cuts at: 0 1004 1500 1569
Size: 1004 496 69
Fragments arranged by size:
1004 496 69
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1042 1075 1569
Size: 1042 33 494
Fragments arranged by size:
1042 494 33
BanI G'GyrC_C
Cuts at: 0 1058 1569
Size: 1058 511
BbsI GAAGACnn'nnnn_
Cuts at: 0 1161 1557 1569
Size: 1161 396 12
Fragments arranged by size:
1161 396 12
BfrBI ATG'CAT
Cuts at: 0 162 1569
Size: 162 1407
Bme1580I G_kGCm'C
Cuts at: 0 693 1569
Size: 693 876
BmtI G_CTAG'C
Cuts at: 0 988 1569
Size: 988 581
BsaI GGTCTCn'nnnn_
Cuts at: 0 207 530 1569
Size: 207 323 1039
Fragments arranged by size:
1039 323 207
BsaAI yAC'GTr
Cuts at: 0 895 1569
Size: 895 674
BsaBI GATnn'nnATC
Cuts at: 0 467 1569
Size: 467 1102
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 938 968 1569
Size: 938 30 601
Fragments arranged by size:
938 601 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 946 1273 1569
Size: 946 327 296
Fragments arranged by size:
946 327 296
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 681 1177 1569
Size: 681 496 392
Fragments arranged by size:
681 496 392
BsiHKAI G_wGCw'C
Cuts at: 0 693 1569
Size: 693 876
BsmI GAATG_Cn'
Cuts at: 0 162 1569
Size: 162 1407
BsmBI CGTCTCn'nnnn_
Cuts at: 0 155 1569
Size: 155 1414
Bsp1286I G_dGCh'C
Cuts at: 0 693 1569
Size: 693 876
BspHI T'CATG_A
Cuts at: 0 291 851 1569
Size: 291 560 718
Fragments arranged by size:
718 560 291
BsrDI GCAATG_nn'
Cuts at: 0 205 263 1569
Size: 205 58 1306
Fragments arranged by size:
1306 205 58
BsrFI r'CCGG_y
Cuts at: 0 1061 1569
Size: 1061 508
BstBI TT'CG_AA
Cuts at: 0 1419 1509 1569
Size: 1419 90 60
Fragments arranged by size:
1419 90 60
BstXI CCAn_nnnn'nTGG
Cuts at: 0 337 1569
Size: 337 1232
BstYI r'GATC_y
Cuts at: 0 187 404 665 1569
Size: 187 217 261 904
Fragments arranged by size:
904 261 217 187
BstZ17I GTA'TAC
Cuts at: 0 394 1569
Size: 394 1175
BtgI C'CryG_G
Cuts at: 0 1178 1569
Size: 1178 391
BtsI GCAGTG_nn'
Cuts at: 0 436 1569
Size: 436 1133
DraI TTT'AAA
Cuts at: 0 422 734 1569
Size: 422 312 835
Fragments arranged by size:
835 422 312
DraIII CAC_nnn'GTG
Cuts at: 0 223 1569
Size: 223 1346
EaeI y'GGCC_r
Cuts at: 0 1063 1237 1569
Size: 1063 174 332
Fragments arranged by size:
1063 332 174
EarI CTCTTCn'nnn_
Cuts at: 0 1388 1569
Size: 1388 181
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1345 1352 1412 1569
Size: 1345 7 60 157
Fragments arranged by size:
1345 157 60 7
EcoO109I rG'GnC_Cy
Cuts at: 0 1497 1569
Size: 1497 72
EcoRV GAT'ATC
Cuts at: 0 469 1569
Size: 469 1100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 477 509 1569
Size: 477 32 1060
Fragments arranged by size:
1060 477 32
HindIII A'AGCT_T
Cuts at: 0 332 589 1569
Size: 332 257 980
Fragments arranged by size:
980 332 257
KpnI G_GTAC'C
Cuts at: 0 1062 1569
Size: 1062 507
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 1230 1569
Size: 169 1061 339
Fragments arranged by size:
1061 339 169
MscI TGG'CCA
Cuts at: 0 1239 1569
Size: 1239 330
MslI CAynn'nnrTG
Cuts at: 0 256 856 1569
Size: 256 600 713
Fragments arranged by size:
713 600 256
MspA1I CmG'CkG
Cuts at: 0 1390 1569
Size: 1390 179
NdeI CA'TA_TG
Cuts at: 0 606 1569
Size: 606 963
NheI G'CTAG_C
Cuts at: 0 984 1569
Size: 984 585
NsiI A_TGCA'T
Cuts at: 0 164 1569
Size: 164 1405
NspI r_CATG'y
Cuts at: 0 742 1116 1411 1444 1563 1569
Size: 742 374 295 33 119 6
Fragments arranged by size:
742 374 295 119 33 6
PciI A'CATG_T
Cuts at: 0 1112 1407 1569
Size: 1112 295 162
Fragments arranged by size:
1112 295 162
PfoI T'CCnGG_A
Cuts at: 0 1167 1569
Size: 1167 402
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1209 1241 1569
Size: 1209 32 328
Fragments arranged by size:
1209 328 32
PpuMI rG'GwC_Cy
Cuts at: 0 1497 1569
Size: 1497 72
PstI C_TGCA'G
Cuts at: 0 447 1569
Size: 447 1122
PvuII CAG'CTG
Cuts at: 0 1390 1569
Size: 1390 179
ScaI AGT'ACT
Cuts at: 0 1096 1569
Size: 1096 473
SfcI C'TryA_G
Cuts at: 0 368 443 1569
Size: 368 75 1126
Fragments arranged by size:
1126 368 75
SmaI CCC'GGG
Cuts at: 0 1502 1569
Size: 1502 67
SphI G_CATG'C
Cuts at: 0 1563 1569
Size: 1563 6
SspI AAT'ATT
Cuts at: 0 1466 1569
Size: 1466 103
StuI AGG'CCT
Cuts at: 0 141 1569
Size: 141 1428
StyI C'CwwG_G
Cuts at: 0 240 1569
Size: 240 1329
SwaI ATTT'AAAT
Cuts at: 0 422 1569
Size: 422 1147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1332 1569
Size: 1332 237
TatI w'GTAC_w
Cuts at: 0 179 827 1094 1412 1569
Size: 179 648 267 318 157
Fragments arranged by size:
648 318 267 179 157
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 938 970 1163 1195 1569
Size: 938 32 193 32 374
Fragments arranged by size:
938 374 193 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 892 1569
Size: 892 677
XmaI C'CCGG_G
Cuts at: 0 1500 1569
Size: 1500 69
XmnI GAAnn'nnTTC
Cuts at: 0 1515 1569
Size: 1515 54
Enzymes that do cut:
AccI Acc65I AcuI AflIII ApaLI ApoI AseI AvaI
BaeI BanI BbsI BfrBI Bme1580I BmtI BsaI BsaAI
BsaBI BsaXI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BsrDI BsrFI BstBI BstXI BstYI BstZ17I BtgI
BtsI DraI DraIII EaeI EarI Eco57MI EcoO109I EcoRV
Hin4I HindIII KpnI MmeI MscI MslI MspA1I NdeI
NheI NsiI NspI PciI PfoI PpiI PpuMI PstI
PvuII ScaI SfcI SmaI SphI SspI StuI StyI
SwaI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI AscI AsiSI AvrII BamHI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaHI
BsaWI BseYI BsiEI BsiWI BspEI BspMI BsrBI BsrGI
BssHII BssSI BstAPI BstEII Bsu36I BtgZI ClaI CspCI
DrdI EagI EciI EcoICRI EcoNI EcoRI FalI FseI
FspI FspAI HaeII HincII HpaI KasI MfeI MluI
NaeI NarI NcoI NgoMIV NotI NruI PacI PasI
PflMI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SrfI Tth111I XbaI XhoI ZraI