Restriction map for
pFN21AB9399
(Linear) (Six-base) MAPSORT of: KIBB9399.seq Check: 3226 from: 1 to: 5373
KIBB9399.orf
With 182 enzymes: SgfI *
April 12, 2013 15:25 ..
AccI GT'mk_AC
Cuts at: 0 2694 2967 3590 5373
Size: 2694 273 623 1783
Fragments arranged by size:
2694 1783 623 273
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 736 1998 2930 2980 4368 4853 5373
Size: 291 445 1262 932 50 1388 485 520
Fragments arranged by size:
1388 1262 932 520 485 445 291 50
AflIII A'CryG_T
Cuts at: 0 2900 4790 5373
Size: 2900 1890 583
Fragments arranged by size:
2900 1890 583
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1887 5373
Size: 1887 3486
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4540 4572 5113 5145 5373
Size: 4540 32 541 32 228
Fragments arranged by size:
4540 541 228 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 448 962 1104 1278 1450 1689 3362 4576
Size: 448 514 142 174 172 239 1673 1214
Cuts at: 4576 4831 5373
Size: 255 542
Fragments arranged by size:
1673 1214 542 514 448 255 239 174
172 142
ApoI r'AATT_y
Cuts at: 0 42 915 925 1020 1836 2779 3607 4287
Size: 42 873 10 95 816 943 828 680
Cuts at: 4287 5373
Size: 1086
Fragments arranged by size:
1086 943 873 828 816 680 95 42
10
AseI AT'TA_AT
Cuts at: 0 998 3623 5373
Size: 998 2625 1750
Fragments arranged by size:
2625 1750 998
AvaI C'yCGr_G
Cuts at: 0 852 5373
Size: 852 4521
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2690 2723 5373
Size: 2690 33 2650
Fragments arranged by size:
2690 2650 33
BamHI G'GATC_C
Cuts at: 0 2937 4018 5373
Size: 2937 1081 1355
Fragments arranged by size:
2937 1355 1081
BanI G'GyrC_C
Cuts at: 0 409 2748 3517 5373
Size: 409 2339 769 1856
Fragments arranged by size:
2339 1856 769 409
BanII G_rGCy'C
Cuts at: 0 218 1121 1162 2100 2671 2846 5373
Size: 218 903 41 938 571 175 2527
Fragments arranged by size:
2527 938 903 571 218 175 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 242 486 1122 1767 2022 2361 4495 5373
Size: 242 244 636 645 255 339 2134 878
Fragments arranged by size:
2134 878 645 636 339 255 244 242
BbvCI CC'TCA_GC
Cuts at: 0 155 790 5373
Size: 155 635 4583
Fragments arranged by size:
4583 635 155
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2073 2107 3828 3862 5373
Size: 2073 34 1721 34 1511
Fragments arranged by size:
2073 1721 1511 34 34
BclI T'GATC_A
Cuts at: 0 2378 5373
Size: 2378 2995
BfrBI ATG'CAT
Cuts at: 0 848 2344 3620 5373
Size: 848 1496 1276 1753
Fragments arranged by size:
1753 1496 1276 848
BglII A'GATC_T
Cuts at: 0 4097 5373
Size: 4097 1276
BlpI GC'TnA_GC
Cuts at: 0 3202 4669 5373
Size: 3202 1467 704
Fragments arranged by size:
3202 1467 704
Bme1580I G_kGCm'C
Cuts at: 0 3216 5373
Size: 3216 2157
BmgBI CAC'GTC
Cuts at: 0 712 5373
Size: 712 4661
BmrI ACTGGGnnnn_n'
Cuts at: 0 13 509 1124 1677 3471 4215 5373
Size: 13 496 615 553 1794 744 1158
Fragments arranged by size:
1794 1158 744 615 553 496 13
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1564 2561 3125 5373
Size: 1564 997 564 2248
Fragments arranged by size:
2248 1564 997 564
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 3328 5293 5373
Size: 159 3169 1965 80
Fragments arranged by size:
3169 1965 159 80
Bpu10I CC'TnA_GC
Cuts at: 0 155 790 2023 2819 4961 5373
Size: 155 635 1233 796 2142 412
Fragments arranged by size:
2142 1233 796 635 412 155
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2569 3259 5134 5373
Size: 2569 690 1875 239
Fragments arranged by size:
2569 1875 690 239
BsaI GGTCTCn'nnnn_
Cuts at: 0 2668 4075 5373
Size: 2668 1407 1298
Fragments arranged by size:
2668 1407 1298
BsaBI GATnn'nnATC
Cuts at: 0 94 5373
Size: 94 5279
BsaHI Gr'CG_yC
Cuts at: 0 2723 5373
Size: 2723 2650
BsaWI w'CCGG_w
Cuts at: 0 3437 5373
Size: 3437 1936
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1225 2698 2776 4447 5373
Size: 1225 1473 78 1671 926
Fragments arranged by size:
1671 1473 1225 926 78
BseYI C'CCAG_C
Cuts at: 0 143 1271 5373
Size: 143 1128 4102
Fragments arranged by size:
4102 1128 143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2592 2606 4421 5373
Size: 2592 14 1815 952
Fragments arranged by size:
2592 1815 952 14
BsiHKAI G_wGCw'C
Cuts at: 0 218 1566 2024 2408 2846 3453 4361 5373
Size: 218 1348 458 384 438 607 908 1012
Fragments arranged by size:
1348 1012 908 607 458 438 384 218
BsmI GAATG_Cn'
Cuts at: 0 2346 5373
Size: 2346 3027
Bsp1286I G_dGCh'C
Cuts at: 0 218 1121 1162 1566 2024 2100 2408 2671
Size: 218 903 41 404 458 76 308 263
Cuts at: 2671 2846 3216 3453 4361 5373
Size: 175 370 237 908 1012
Fragments arranged by size:
1012 908 903 458 404 370 308 263
237 218 175 76 41
BspEI T'CCGG_A
Cuts at: 0 3437 5373
Size: 3437 1936
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3140 3151 3534 4642 5373
Size: 3140 11 383 1108 731
Fragments arranged by size:
3140 1108 731 383 11
BsrBI CCG'CTC
Cuts at: 0 39 5373
Size: 39 5334
BsrDI GCAATG_nn'
Cuts at: 0 479 894 1883 2417 2451 4010 4796 5120
Size: 479 415 989 534 34 1559 786 324
Cuts at: 5120 5328 5373
Size: 208 45
Fragments arranged by size:
1559 989 786 534 479 415 324 208
45 34
BsrGI T'GTAC_A
Cuts at: 0 4793 5373
Size: 4793 580
BssSI C'ACGA_G
Cuts at: 0 209 1663 2868 4072 4439 5373
Size: 209 1454 1205 1204 367 934
Fragments arranged by size:
1454 1205 1204 934 367 209
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1570 2294 2755 5373
Size: 1570 724 461 2618
Fragments arranged by size:
2618 1570 724 461
BstBI TT'CG_AA
Cuts at: 0 5232 5373
Size: 5232 141
BstEII G'GTnAC_C
Cuts at: 0 3014 5250 5260 5269 5373
Size: 3014 2236 10 9 104
Fragments arranged by size:
3014 2236 104 10 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 587 1438 4729 5373
Size: 587 851 3291 644
Fragments arranged by size:
3291 851 644 587
BstYI r'GATC_y
Cuts at: 0 576 1542 2937 4018 4097 5373
Size: 576 966 1395 1081 79 1276
Fragments arranged by size:
1395 1276 1081 966 576 79
BstZ17I GTA'TAC
Cuts at: 0 2695 2968 3591 5373
Size: 2695 273 623 1782
Fragments arranged by size:
2695 1782 623 273
Bsu36I CC'TnA_GG
Cuts at: 0 4420 5373
Size: 4420 953
BtgI C'CryG_G
Cuts at: 0 580 1199 1310 1784 4045 5018 5373
Size: 580 619 111 474 2261 973 355
Fragments arranged by size:
2261 973 619 580 474 355 111
BtsI GCAGTG_nn'
Cuts at: 0 3213 5040 5373
Size: 3213 1827 333
Fragments arranged by size:
3213 1827 333
ClaI AT'CG_AT
Cuts at: 0 2511 4583 5373
Size: 2511 2072 790
Fragments arranged by size:
2511 2072 790
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 918 953 5373
Size: 918 35 4420
Fragments arranged by size:
4420 918 35
DraIII CAC_nnn'GTG
Cuts at: 0 1570 5373
Size: 1570 3803
DrdI GACnn_nn'nnGTC
Cuts at: 0 601 4303 5373
Size: 601 3702 1070
Fragments arranged by size:
3702 1070 601
EaeI y'GGCC_r
Cuts at: 0 1742 1781 1996 2050 2169 3957 4410 5348
Size: 1742 39 215 54 119 1788 453 938
Cuts at: 5348 5373
Size: 25
Fragments arranged by size:
1788 1742 938 453 215 119 54 39
25
EarI CTCTTCn'nnn_
Cuts at: 0 826 1952 2419 3467 3910 4387 4636 4710
Size: 826 1126 467 1048 443 477 249 74
Cuts at: 4710 5117 5189 5373
Size: 407 72 184
Fragments arranged by size:
1126 1048 826 477 467 443 407 249
184 74 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 37 2506 5373
Size: 37 2469 2867
Fragments arranged by size:
2867 2469 37
EcoICRI GAG'CTC
Cuts at: 0 216 2844 5373
Size: 216 2628 2529
Fragments arranged by size:
2628 2529 216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 291 736 1998 2930 2980 3328 4368
Size: 159 132 445 1262 932 50 348 1040
Cuts at: 4368 4853 5293 5373
Size: 485 440 80
Fragments arranged by size:
1262 1040 932 485 445 440 348 159
132 80 50
EcoO109I rG'GnC_Cy
Cuts at: 0 445 731 1704 4845 5373
Size: 445 286 973 3141 528
Fragments arranged by size:
3141 973 528 445 286
EcoRI G'AATT_C
Cuts at: 0 925 5373
Size: 925 4448
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 339 1422 2601 5373
Size: 339 1083 1179 2772
Fragments arranged by size:
2772 1179 1083 339
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 362 394 2890 2922 5016 5048 5373
Size: 362 32 2496 32 2094 32 325
Fragments arranged by size:
2496 2094 362 325 32 32 32
HincII GTy'rAC
Cuts at: 0 762 3714 4788 5373
Size: 762 2952 1074 585
Fragments arranged by size:
2952 1074 762 585
HindIII A'AGCT_T
Cuts at: 0 518 884 1087 5373
Size: 518 366 203 4286
Fragments arranged by size:
4286 518 366 203
MfeI C'AATT_G
Cuts at: 0 77 5373
Size: 77 5296
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 2073 4399 5373
Size: 86 1987 2326 974
Fragments arranged by size:
2326 1987 974 86
MscI TGG'CCA
Cuts at: 0 1744 1783 1998 2052 3959 5350 5373
Size: 1744 39 215 54 1907 1391 23
Fragments arranged by size:
1907 1744 1391 215 54 39 23
MslI CAynn'nnrTG
Cuts at: 0 946 1529 1594 2355 2656 3385 3970 4801
Size: 946 583 65 761 301 729 585 831
Cuts at: 4801 5373
Size: 572
Fragments arranged by size:
946 831 761 729 585 583 572 301
65
MspA1I CmG'CkG
Cuts at: 0 1278 3167 3796 5373
Size: 1278 1889 629 1577
Fragments arranged by size:
1889 1577 1278 629
NcoI C'CATG_G
Cuts at: 0 580 1199 1310 1784 5018 5373
Size: 580 619 111 474 3234 355
Fragments arranged by size:
3234 619 580 474 355 111
NdeI CA'TA_TG
Cuts at: 0 1206 5373
Size: 1206 4167
NsiI A_TGCA'T
Cuts at: 0 850 2346 3622 5373
Size: 850 1496 1276 1751
Fragments arranged by size:
1751 1496 1276 850
NspI r_CATG'y
Cuts at: 0 322 399 2160 2904 4623 4794 5373
Size: 322 77 1761 744 1719 171 579
Fragments arranged by size:
1761 1719 744 579 322 171 77
PasI CC'CwG_GG
Cuts at: 0 2718 3113 5373
Size: 2718 395 2260
Fragments arranged by size:
2718 2260 395
PciI A'CATG_T
Cuts at: 0 2900 4790 5373
Size: 2900 1890 583
Fragments arranged by size:
2900 1890 583
PflMI CCAn_nnn'nTGG
Cuts at: 0 2048 3362 4283 4576 5046 5373
Size: 2048 1314 921 293 470 327
Fragments arranged by size:
2048 1314 921 470 327 293
PfoI T'CCnGG_A
Cuts at: 0 175 633 3305 5373
Size: 175 458 2672 2068
Fragments arranged by size:
2672 2068 458 175
PpuMI rG'GwC_Cy
Cuts at: 0 1704 4845 5373
Size: 1704 3141 528
Fragments arranged by size:
3141 1704 528
PshAI GACnn'nnGTC
Cuts at: 0 2116 5373
Size: 2116 3257
PsiI TTA'TAA
Cuts at: 0 3643 5373
Size: 3643 1730
PstI C_TGCA'G
Cuts at: 0 1236 5373
Size: 1236 4137
PvuII CAG'CTG
Cuts at: 0 1278 3167 3796 5373
Size: 1278 1889 629 1577
Fragments arranged by size:
1889 1577 1278 629
SacI G_AGCT'C
Cuts at: 0 218 2846 5373
Size: 218 2628 2527
Fragments arranged by size:
2628 2527 218
SapI GCTCTTCn'nnn_
Cuts at: 0 826 1952 4387 5373
Size: 826 1126 2435 986
Fragments arranged by size:
2435 1126 986 826
SfcI C'TryA_G
Cuts at: 0 440 1232 3087 5373
Size: 440 792 1855 2286
Fragments arranged by size:
2286 1855 792 440
SmlI C'TyrA_G
Cuts at: 0 852 2548 3238 5149 5373
Size: 852 1696 690 1911 224
Fragments arranged by size:
1911 1696 852 690 224
SphI G_CATG'C
Cuts at: 0 322 2160 4623 5373
Size: 322 1838 2463 750
Fragments arranged by size:
2463 1838 750 322
SspI AAT'ATT
Cuts at: 0 533 4927 5373
Size: 533 4394 446
Fragments arranged by size:
4394 533 446
StuI AGG'CCT
Cuts at: 0 172 2836 3459 5227 5373
Size: 172 2664 623 1768 146
Fragments arranged by size:
2664 1768 623 172 146
StyI C'CwwG_G
Cuts at: 0 580 1199 1247 1310 1784 2100 4343 5018
Size: 580 619 48 63 474 316 2243 675
Cuts at: 5018 5255 5265 5351 5373
Size: 237 10 86 22
Fragments arranged by size:
2243 675 619 580 474 316 237 86
63 48 22 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 4843 5373
Size: 4843 530
TatI w'GTAC_w
Cuts at: 0 1977 3274 4327 4793 5237 5373
Size: 1977 1297 1053 466 444 136
Fragments arranged by size:
1977 1297 1053 466 444 136
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2384 2416 3479 3511 5373
Size: 2384 32 1063 32 1862
Fragments arranged by size:
2384 1862 1063 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 4084 5373
Size: 4084 1289
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2686 5373
Size: 2686 2687
XhoI C'TCGA_G
Cuts at: 0 852 5373
Size: 852 4521
XmnI GAAnn'nnTTC
Cuts at: 0 1402 3230 3433 3904 5373
Size: 1402 1828 203 471 1469
Fragments arranged by size:
1828 1469 1402 471 203
Enzymes that do cut:
AccI AcuI AflIII AlfI AloI AlwNI ApoI AseI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I
NcoI NdeI NsiI NspI PasI PciI PflMI PfoI
PpuMI PshAI PsiI PstI PvuII SacI SapI SfcI
SmlI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI ApaI ApaLI AscI AsiSI AvrII BbeI BciVI
BglI BmtI BsaAI BsaXI BsiEI BsiWI BsmBI BspHI
BsrFI BssHII BtgZI DraI EagI EcoNI EcoRV FseI
FspI FspAI HaeII HpaI KasI KpnI MluI NaeI
NarI NgoMIV NheI NotI NruI PacI PmeI PmlI
PpiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SwaI XbaI XmaI ZraI