Restriction map for
pFN21AB9292
(Linear) (Six-base) MAPSORT of: KIBB9292.seq Check: 6137 from: 1 to: 810
KIBB9292.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AfeI AGC'GCT
Cuts at: 0 40 810
Size: 40 770
AhdI GACnn_n'nnGTC
Cuts at: 0 183 810
Size: 183 627
AleI CACnn'nnGTG
Cuts at: 0 222 810
Size: 222 588
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 240 482 566 810
Size: 240 242 84 244
Fragments arranged by size:
244 242 240 84
ApaI G_GGCC'C
Cuts at: 0 160 214 300 810
Size: 160 54 86 510
Fragments arranged by size:
510 160 86 54
ApaLI G'TGCA_C
Cuts at: 0 628 810
Size: 628 182
AvaI C'yCGr_G
Cuts at: 0 6 810
Size: 6 804
AvrII C'CTAG_G
Cuts at: 0 102 810
Size: 102 708
BanI G'GyrC_C
Cuts at: 0 202 810
Size: 202 608
BanII G_rGCy'C
Cuts at: 0 160 214 300 714 810
Size: 160 54 86 414 96
Fragments arranged by size:
414 160 96 86 54
BbeI G_GCGC'C
Cuts at: 0 206 810
Size: 206 604
BbsI GAAGACnn'nnnn_
Cuts at: 0 604 675 810
Size: 604 71 135
Fragments arranged by size:
604 135 71
BglI GCCn_nnn'nGGC
Cuts at: 0 164 810
Size: 164 646
Bme1580I G_kGCm'C
Cuts at: 0 160 214 300 632 810
Size: 160 54 86 332 178
Fragments arranged by size:
332 178 160 86 54
BmrI ACTGGGnnnn_n'
Cuts at: 0 162 810
Size: 162 648
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 810
Size: 139 671
BsaHI Gr'CG_yC
Cuts at: 0 184 203 810
Size: 184 19 607
Fragments arranged by size:
607 184 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 159 286 319 747 810
Size: 76 83 127 33 428 63
Fragments arranged by size:
428 127 83 76 63 33
BseYI C'CCAG_C
Cuts at: 0 56 563 810
Size: 56 507 247
Fragments arranged by size:
507 247 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 810
Size: 76 734
BsiEI CG_ry'CG
Cuts at: 0 164 810
Size: 164 646
BsiHKAI G_wGCw'C
Cuts at: 0 632 714 810
Size: 632 82 96
Fragments arranged by size:
632 96 82
Bsp1286I G_dGCh'C
Cuts at: 0 160 214 300 632 714 810
Size: 160 54 86 332 82 96
Fragments arranged by size:
332 160 96 86 82 54
BspHI T'CATG_A
Cuts at: 0 639 810
Size: 639 171
BsrBI CCG'CTC
Cuts at: 0 14 622 810
Size: 14 608 188
Fragments arranged by size:
608 188 14
BsrFI r'CCGG_y
Cuts at: 0 110 163 258 810
Size: 110 53 95 552
Fragments arranged by size:
552 110 95 53
BssHII G'CGCG_C
Cuts at: 0 289 810
Size: 289 521
BssSI C'ACGA_G
Cuts at: 0 631 810
Size: 631 179
BtgI C'CryG_G
Cuts at: 0 769 810
Size: 769 41
BtsI GCAGTG_nn'
Cuts at: 0 367 810
Size: 367 443
DraIII CAC_nnn'GTG
Cuts at: 0 720 810
Size: 720 90
EaeI y'GGCC_r
Cuts at: 0 161 810
Size: 161 649
EagI C'GGCC_G
Cuts at: 0 161 810
Size: 161 649
EarI CTCTTCn'nnn_
Cuts at: 0 16 810
Size: 16 794
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 292 810
Size: 292 518
EcoICRI GAG'CTC
Cuts at: 0 712 810
Size: 712 98
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 810
Size: 139 671
EcoO109I rG'GnC_Cy
Cuts at: 0 156 210 211 296 297 810
Size: 156 54 1 85 1 513
Fragments arranged by size:
513 156 85 54 1 1
HaeII r_GCGC'y
Cuts at: 0 42 206 810
Size: 42 164 604
Fragments arranged by size:
604 164 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 169 201 810
Size: 169 32 609
Fragments arranged by size:
609 169 32
KasI G'GCGC_C
Cuts at: 0 202 810
Size: 202 608
MslI CAynn'nnrTG
Cuts at: 0 222 810
Size: 222 588
NaeI GCC'GGC
Cuts at: 0 112 165 260 810
Size: 112 53 95 550
Fragments arranged by size:
550 112 95 53
NarI GG'CG_CC
Cuts at: 0 203 810
Size: 203 607
NcoI C'CATG_G
Cuts at: 0 769 810
Size: 769 41
NgoMIV G'CCGG_C
Cuts at: 0 110 163 258 810
Size: 110 53 95 552
Fragments arranged by size:
552 110 95 53
PspOMI G'GGCC_C
Cuts at: 0 156 210 296 810
Size: 156 54 86 514
Fragments arranged by size:
514 156 86 54
SacI G_AGCT'C
Cuts at: 0 714 810
Size: 714 96
SapI GCTCTTCn'nnn_
Cuts at: 0 16 810
Size: 16 794
ScaI AGT'ACT
Cuts at: 0 444 810
Size: 444 366
SfoI GGC'GCC
Cuts at: 0 204 810
Size: 204 606
SmaI CCC'GGG
Cuts at: 0 8 810
Size: 8 802
StyI C'CwwG_G
Cuts at: 0 102 769 810
Size: 102 667 41
Fragments arranged by size:
667 102 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 450 595 810
Size: 450 145 215
Fragments arranged by size:
450 215 145
TatI w'GTAC_w
Cuts at: 0 29 442 810
Size: 29 413 368
Fragments arranged by size:
413 368 29
XmaI C'CCGG_G
Cuts at: 0 6 810
Size: 6 804
Enzymes that do cut:
AfeI AhdI AleI AlfI ApaI ApaLI AvaI AvrII
BanI BanII BbeI BbsI BglI Bme1580I BmrI BpmI
BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI
BsrBI BsrFI BssHII BssSI BtgI BtsI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I
KasI MslI NaeI NarI NcoI NgoMIV PspOMI SacI
SapI ScaI SfoI SmaI StyI TaqII TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AloI AlwNI ApoI AscI AseI AsiSI BaeI
BamHI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspMI BsrDI
BsrGI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MspA1I NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI PvuII RsrII SacII SalI SanDI SbfI
SexAI SfcI SfiI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XcmI XhoI XmnI ZraI