Restriction map for
pFN21AB9130
(Linear) (Six-base) MAPSORT of: KIBB9130.seq Check: 9801 from: 1 to: 2283
KIBB9130.orf
With 182 enzymes: SgfI *
January 15, 2009 16:32 ..
AccI GT'mk_AC
Cuts at: 0 455 1325 2283
Size: 455 870 958
Fragments arranged by size:
958 870 455
Acc65I G'GTAC_C
Cuts at: 0 369 2283
Size: 369 1914
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 224 290 620 743 779 2283
Size: 158 66 66 330 123 36 1504
Fragments arranged by size:
1504 330 158 123 66 66 36
AflII C'TTAA_G
Cuts at: 0 1813 2283
Size: 1813 470
AflIII A'CryG_T
Cuts at: 0 426 2163 2283
Size: 426 1737 120
Fragments arranged by size:
1737 426 120
AleI CACnn'nnGTG
Cuts at: 0 1770 1859 2199 2201 2283
Size: 1770 89 340 2 82
Fragments arranged by size:
1770 340 89 82 2
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 416 448 2283
Size: 416 32 1835
Fragments arranged by size:
1835 416 32
AlwNI CAG_nnn'CTG
Cuts at: 0 136 238 511 829 1952 2283
Size: 136 102 273 318 1123 331
Fragments arranged by size:
1123 331 318 273 136 102
ApoI r'AATT_y
Cuts at: 0 1022 1339 1503 2206 2283
Size: 1022 317 164 703 77
Fragments arranged by size:
1022 703 317 164 77
AseI AT'TA_AT
Cuts at: 0 1037 2283
Size: 1037 1246
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 455 488 2283
Size: 455 33 1795
Fragments arranged by size:
1795 455 33
BanI G'GyrC_C
Cuts at: 0 369 2261 2283
Size: 369 1892 22
Fragments arranged by size:
1892 369 22
BanII G_rGCy'C
Cuts at: 0 1901 2283
Size: 1901 382
BbsI GAAGACnn'nnnn_
Cuts at: 0 608 2092 2235 2283
Size: 608 1484 143 48
Fragments arranged by size:
1484 608 143 48
BclI T'GATC_A
Cuts at: 0 186 327 1218 2283
Size: 186 141 891 1065
Fragments arranged by size:
1065 891 186 141
BfrBI ATG'CAT
Cuts at: 0 2146 2283
Size: 2146 137
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 851 2283
Size: 548 303 1432
Fragments arranged by size:
1432 548 303
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 881 1543 2283
Size: 881 662 740
Fragments arranged by size:
881 740 662
BsaI GGTCTCn'nnnn_
Cuts at: 0 107 2283
Size: 107 2176
BsaBI GATnn'nnATC
Cuts at: 0 326 2283
Size: 326 1957
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 493 1089 2283
Size: 493 596 1194
Fragments arranged by size:
1194 596 493
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 1646 1965 2283
Size: 83 1563 319 318
Fragments arranged by size:
1563 319 318 83
BsiHKAI G_wGCw'C
Cuts at: 0 595 1798 1901 2283
Size: 595 1203 103 382
Fragments arranged by size:
1203 595 382 103
BsmI GAATG_Cn'
Cuts at: 0 857 2283
Size: 857 1426
Bsp1286I G_dGCh'C
Cuts at: 0 595 1798 1901 2283
Size: 595 1203 103 382
Fragments arranged by size:
1203 595 382 103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 369 2283
Size: 369 1914
BsrGI T'GTAC_A
Cuts at: 0 1246 2283
Size: 1246 1037
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1711 2283
Size: 1711 572
BtgI C'CryG_G
Cuts at: 0 213 2283
Size: 213 2070
BtsI GCAGTG_nn'
Cuts at: 0 684 1102 2283
Size: 684 418 1181
Fragments arranged by size:
1181 684 418
ClaI AT'CG_AT
Cuts at: 0 107 2283
Size: 107 2176
DraI TTT'AAA
Cuts at: 0 747 1176 1962 2157 2283
Size: 747 429 786 195 126
Fragments arranged by size:
786 747 429 195 126
EaeI y'GGCC_r
Cuts at: 0 1487 1989 2283
Size: 1487 502 294
Fragments arranged by size:
1487 502 294
EarI CTCTTCn'nnn_
Cuts at: 0 296 921 2283
Size: 296 625 1362
Fragments arranged by size:
1362 625 296
EcoICRI GAG'CTC
Cuts at: 0 1899 2283
Size: 1899 384
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 224 290 548 620 743 779 851
Size: 158 66 66 258 72 123 36 72
Cuts at: 851 2283
Size: 1432
Fragments arranged by size:
1432 258 158 123 72 72 66 66
36
EcoO109I rG'GnC_Cy
Cuts at: 0 1120 2283
Size: 1120 1163
EcoRI G'AATT_C
Cuts at: 0 2206 2283
Size: 2206 77
EcoRV GAT'ATC
Cuts at: 0 225 617 912 1917 2171 2283
Size: 225 392 295 1005 254 112
Fragments arranged by size:
1005 392 295 254 225 112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 295 2283
Size: 295 1988
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 209 241 1121 1153 2283
Size: 209 32 880 32 1130
Fragments arranged by size:
1130 880 209 32 32
HincII GTy'rAC
Cuts at: 0 456 1326 2283
Size: 456 870 957
Fragments arranged by size:
957 870 456
KpnI G_GTAC'C
Cuts at: 0 373 2283
Size: 373 1910
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 612 2283
Size: 612 1671
MscI TGG'CCA
Cuts at: 0 1991 2283
Size: 1991 292
MslI CAynn'nnrTG
Cuts at: 0 64 107 1517 1583 1599 1686 1770 1859
Size: 64 43 1410 66 16 87 84 89
Cuts at: 1859 2028 2199 2201 2283
Size: 169 171 2 82
Fragments arranged by size:
1410 171 169 89 87 84 82 66
64 43 16 2
MspA1I CmG'CkG
Cuts at: 0 202 526 858 1108 2283
Size: 202 324 332 250 1175
Fragments arranged by size:
1175 332 324 250 202
NcoI C'CATG_G
Cuts at: 0 213 2283
Size: 213 2070
NdeI CA'TA_TG
Cuts at: 0 650 1568 2081 2283
Size: 650 918 513 202
Fragments arranged by size:
918 650 513 202
NsiI A_TGCA'T
Cuts at: 0 2148 2283
Size: 2148 135
NspI r_CATG'y
Cuts at: 0 430 2167 2283
Size: 430 1737 116
Fragments arranged by size:
1737 430 116
PciI A'CATG_T
Cuts at: 0 426 2163 2283
Size: 426 1737 120
Fragments arranged by size:
1737 426 120
PflMI CCAn_nnn'nTGG
Cuts at: 0 1097 2283
Size: 1097 1186
PpuMI rG'GwC_Cy
Cuts at: 0 1120 2283
Size: 1120 1163
PsiI TTA'TAA
Cuts at: 0 730 1114 2283
Size: 730 384 1169
Fragments arranged by size:
1169 730 384
PstI C_TGCA'G
Cuts at: 0 1458 2283
Size: 1458 825
PvuII CAG'CTG
Cuts at: 0 202 526 1108 2283
Size: 202 324 582 1175
Fragments arranged by size:
1175 582 324 202
SacI G_AGCT'C
Cuts at: 0 1901 2283
Size: 1901 382
SalI G'TCGA_C
Cuts at: 0 454 1324 2283
Size: 454 870 959
Fragments arranged by size:
959 870 454
ScaI AGT'ACT
Cuts at: 0 1052 2283
Size: 1052 1231
SfcI C'TryA_G
Cuts at: 0 545 1454 2138 2283
Size: 545 909 684 145
Fragments arranged by size:
909 684 545 145
SmlI C'TyrA_G
Cuts at: 0 896 1558 1813 2283
Size: 896 662 255 470
Fragments arranged by size:
896 662 470 255
StyI C'CwwG_G
Cuts at: 0 213 2283
Size: 213 2070
TatI w'GTAC_w
Cuts at: 0 806 1000 1050 1246 2283
Size: 806 194 50 196 1037
Fragments arranged by size:
1037 806 196 194 50
XbaI T'CTAG_A
Cuts at: 0 43 2283
Size: 43 2240
XmnI GAAnn'nnTTC
Cuts at: 0 930 2221 2283
Size: 930 1291 62
Fragments arranged by size:
1291 930 62
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AleI AloI AlwNI
ApoI AseI BaeI BanI BanII BbsI BclI BfrBI
BpmI BpuEI BsaI BsaBI BseRI BsgI BsiHKAI BsmI
Bsp1286I BspMI BsrGI BstXI BtgI BtsI ClaI DraI
EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
Hin4I HincII KpnI MmeI MscI MslI MspA1I NcoI
NdeI NsiI NspI PciI PflMI PpuMI PsiI PstI
PvuII SacI SalI ScaI SfcI SmlI StyI TatI
XbaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AgeI AhdI AlfI ApaI
ApaLI AscI AsiSI AvaI AvrII BamHI BbeI BbvCI
BcgI BciVI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI Bpu10I BsaAI BsaHI BsaWI BsaXI BseYI
BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgZI CspCI DraIII DrdI EagI EciI EcoNI FseI
FspI FspAI HaeII HindIII HpaI KasI MfeI MluI
NaeI NarI NgoMIV NheI NotI NruI PacI PasI
PfoI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI
PvuI RsrII SacII SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TstI Tth111I XcmI XhoI
XmaI ZraI