Restriction map for
pFN21AB8983
(Linear) (Six-base) MAPSORT of: KIBB8983.seq Check: 6721 from: 1 to: 1152
KIBB8983.orf
With 182 enzymes: SgfI *
December 5, 2014 12:54 ..
AfeI AGC'GCT
Cuts at: 0 649 1152
Size: 649 503
ApaI G_GGCC'C
Cuts at: 0 69 941 1060 1152
Size: 69 872 119 92
Fragments arranged by size:
872 119 92 69
AvaI C'yCGr_G
Cuts at: 0 69 184 522 550 643 709 901 1021
Size: 69 115 338 28 93 66 192 120
Cuts at: 1021 1152
Size: 131
Fragments arranged by size:
338 192 131 120 115 93 69 66
28
BanI G'GyrC_C
Cuts at: 0 610 705 828 853 1152
Size: 610 95 123 25 299
Fragments arranged by size:
610 299 123 95 25
BanII G_rGCy'C
Cuts at: 0 69 509 674 792 941 1028 1060 1152
Size: 69 440 165 118 149 87 32 92
Fragments arranged by size:
440 165 149 118 92 87 69 32
BbeI G_GCGC'C
Cuts at: 0 614 709 832 1152
Size: 614 95 123 320
Fragments arranged by size:
614 320 123 95
BbvCI CC'TCA_GC
Cuts at: 0 205 1152
Size: 205 947
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 227 261 1152
Size: 227 34 891
Fragments arranged by size:
891 227 34
BglI GCCn_nnn'nGGC
Cuts at: 0 215 936 1152
Size: 215 721 216
Fragments arranged by size:
721 216 215
BlpI GC'TnA_GC
Cuts at: 0 311 343 1152
Size: 311 32 809
Fragments arranged by size:
809 311 32
Bme1580I G_kGCm'C
Cuts at: 0 69 865 941 1060 1152
Size: 69 796 76 119 92
Fragments arranged by size:
796 119 92 76 69
BmgBI CAC'GTC
Cuts at: 0 963 1152
Size: 963 189
BmrI ACTGGGnnnn_n'
Cuts at: 0 1063 1152
Size: 1063 89
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 688 937 1152
Size: 523 165 249 215
Fragments arranged by size:
523 249 215 165
Bpu10I CC'TnA_GC
Cuts at: 0 205 808 1152
Size: 205 603 344
Fragments arranged by size:
603 344 205
BsaAI yAC'GTr
Cuts at: 0 1038 1152
Size: 1038 114
BsaHI Gr'CG_yC
Cuts at: 0 568 611 706 829 1016 1112 1152
Size: 568 43 95 123 187 96 40
Fragments arranged by size:
568 187 123 96 95 43 40
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1096 1152
Size: 1096 56
BseYI C'CCAG_C
Cuts at: 0 154 354 606 933 1152
Size: 154 200 252 327 219
Fragments arranged by size:
327 252 219 200 154
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 448 1152
Size: 313 135 704
Fragments arranged by size:
704 313 135
BsiEI CG_ry'CG
Cuts at: 0 215 219 466 1152
Size: 215 4 247 686
Fragments arranged by size:
686 247 215 4
BsiHKAI G_wGCw'C
Cuts at: 0 344 1152
Size: 344 808
Bsp1286I G_dGCh'C
Cuts at: 0 69 344 509 674 792 865 941 1028
Size: 69 275 165 165 118 73 76 87
Cuts at: 1028 1060 1152
Size: 32 92
Fragments arranged by size:
275 165 165 118 92 87 76 73
69 32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 335 1152
Size: 335 817
BsrBI CCG'CTC
Cuts at: 0 834 1152
Size: 834 318
BsrFI r'CCGG_y
Cuts at: 0 109 214 461 1152
Size: 109 105 247 691
Fragments arranged by size:
691 247 109 105
BssHII G'CGCG_C
Cuts at: 0 484 719 737 1062 1152
Size: 484 235 18 325 90
Fragments arranged by size:
484 325 235 90 18
BstAPI GCAn_nnn'nTGC
Cuts at: 0 435 1152
Size: 435 717
BtgI C'CryG_G
Cuts at: 0 219 563 701 824 1152
Size: 219 344 138 123 328
Fragments arranged by size:
344 328 219 138 123
EaeI y'GGCC_r
Cuts at: 0 212 216 463 1046 1152
Size: 212 4 247 583 106
Fragments arranged by size:
583 247 212 106 4
EagI C'GGCC_G
Cuts at: 0 212 216 463 1152
Size: 212 4 247 689
Fragments arranged by size:
689 247 212 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 400 814 1152
Size: 400 414 338
Fragments arranged by size:
414 400 338
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 688 937 1152
Size: 523 165 249 215
Fragments arranged by size:
523 249 215 165
EcoNI CCTnn'n_nnAGG
Cuts at: 0 799 1152
Size: 799 353
EcoO109I rG'GnC_Cy
Cuts at: 0 66 768 906 937 1152
Size: 66 702 138 31 215
Fragments arranged by size:
702 215 138 66 31
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 414 1152
Size: 414 738
FseI GG_CCGG'CC
Cuts at: 0 218 1152
Size: 218 934
HaeII r_GCGC'y
Cuts at: 0 198 535 614 651 709 808 832 926
Size: 198 337 79 37 58 99 24 94
Cuts at: 926 1152
Size: 226
Fragments arranged by size:
337 226 198 99 94 79 58 37
24
KasI G'GCGC_C
Cuts at: 0 610 705 828 1152
Size: 610 95 123 324
Fragments arranged by size:
610 324 123 95
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1088 1152
Size: 1088 64
MscI TGG'CCA
Cuts at: 0 1048 1152
Size: 1048 104
MspA1I CmG'CkG
Cuts at: 0 88 221 565 606 703 826 888 915
Size: 88 133 344 41 97 123 62 27
Cuts at: 915 933 1152
Size: 18 219
Fragments arranged by size:
344 219 133 123 97 88 62 41
27 18
NaeI GCC'GGC
Cuts at: 0 111 216 1152
Size: 111 105 936
Fragments arranged by size:
936 111 105
NarI GG'CG_CC
Cuts at: 0 611 706 829 1152
Size: 611 95 123 323
Fragments arranged by size:
611 323 123 95
NgoMIV G'CCGG_C
Cuts at: 0 109 214 1152
Size: 109 105 938
Fragments arranged by size:
938 109 105
PasI CC'CwG_GG
Cuts at: 0 858 1152
Size: 858 294
PmlI CAC'GTG
Cuts at: 0 1038 1152
Size: 1038 114
PpuMI rG'GwC_Cy
Cuts at: 0 768 1152
Size: 768 384
PspOMI G'GGCC_C
Cuts at: 0 65 937 1056 1152
Size: 65 872 119 96
Fragments arranged by size:
872 119 96 65
PstI C_TGCA'G
Cuts at: 0 1000 1152
Size: 1000 152
SacII CC_GC'GG
Cuts at: 0 222 566 704 827 1152
Size: 222 344 138 123 325
Fragments arranged by size:
344 325 222 138 123
SanDI GG'GwC_CC
Cuts at: 0 768 1152
Size: 768 384
SfcI C'TryA_G
Cuts at: 0 996 1152
Size: 996 156
SfoI GGC'GCC
Cuts at: 0 612 707 830 1152
Size: 612 95 123 322
Fragments arranged by size:
612 322 123 95
SmaI CCC'GGG
Cuts at: 0 71 524 1023 1152
Size: 71 453 499 129
Fragments arranged by size:
499 453 129 71
TatI w'GTAC_w
Cuts at: 0 845 1152
Size: 845 307
XmaI C'CCGG_G
Cuts at: 0 69 522 1021 1152
Size: 69 453 499 131
Fragments arranged by size:
499 453 131 69
XmnI GAAnn'nnTTC
Cuts at: 0 271 1152
Size: 271 881
Enzymes that do cut:
AfeI ApaI AvaI BanI BanII BbeI BbvCI BcgI
BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaAI
BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrBI BsrFI BssHII BstAPI BtgI EaeI EagI EciI
Eco57MI EcoNI EcoO109I FalI FseI HaeII KasI MmeI
MscI MspA1I NaeI NarI NgoMIV PasI PmlI PpuMI
PspOMI PstI SacII SanDI SfcI SfoI SmaI TatI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BciVI
BclI BfrBI BglII BmtI BplI BpuEI BsaI BsaBI
BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrGI BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI
EcoICRI EcoRI EcoRV FspI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MluI MslI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI ZraI