Restriction map for
pFN21AB8981
(Linear) (Six-base) MAPSORT of: KIBB8981.seq Check: 6277 from: 1 to: 1131
KIBB8981.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 959 1028 1131
Size: 959 69 103
Fragments arranged by size:
959 103 69
AlwNI CAG_nnn'CTG
Cuts at: 0 1051 1131
Size: 1051 80
ApaLI G'TGCA_C
Cuts at: 0 32 1131
Size: 32 1099
ApoI r'AATT_y
Cuts at: 0 247 329 471 1064 1131
Size: 247 82 142 593 67
Fragments arranged by size:
593 247 142 82 67
BbsI GAAGACnn'nnnn_
Cuts at: 0 878 1131
Size: 878 253
BbvCI CC'TCA_GC
Cuts at: 0 546 615 684 753 822 1131
Size: 546 69 69 69 69 309
Fragments arranged by size:
546 309 69 69 69 69
Bme1580I G_kGCm'C
Cuts at: 0 36 1131
Size: 36 1095
BmtI G_CTAG'C
Cuts at: 0 157 1131
Size: 157 974
Bpu10I CC'TnA_GC
Cuts at: 0 215 546 615 684 753 822 1131
Size: 215 331 69 69 69 69 309
Fragments arranged by size:
331 309 215 69 69 69 69
BsaI GGTCTCn'nnnn_
Cuts at: 0 553 622 691 760 829 898 1131
Size: 553 69 69 69 69 69 233
Fragments arranged by size:
553 233 69 69 69 69 69
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 516 546 585 615 654 684 723 753
Size: 516 30 39 30 39 30 39 30
Cuts at: 753 861 891 1032 1062 1131
Size: 108 30 141 30 69
Fragments arranged by size:
516 141 108 69 39 39 39 30
30 30 30 30 30
BseYI C'CCAG_C
Cuts at: 0 477 891 1131
Size: 477 414 240
Fragments arranged by size:
477 414 240
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 965 1131
Size: 181 784 166
Fragments arranged by size:
784 181 166
BsiHKAI G_wGCw'C
Cuts at: 0 36 1131
Size: 36 1095
BsmI GAATG_Cn'
Cuts at: 0 408 1131
Size: 408 723
Bsp1286I G_dGCh'C
Cuts at: 0 36 1131
Size: 36 1095
BspHI T'CATG_A
Cuts at: 0 78 1131
Size: 78 1053
BstAPI GCAn_nnn'nTGC
Cuts at: 0 40 1131
Size: 40 1091
BtsI GCAGTG_nn'
Cuts at: 0 300 1131
Size: 300 831
DraIII CAC_nnn'GTG
Cuts at: 0 40 1131
Size: 40 1091
EaeI y'GGCC_r
Cuts at: 0 1053 1131
Size: 1053 78
EarI CTCTTCn'nnn_
Cuts at: 0 450 1131
Size: 450 681
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 807 1131
Size: 807 324
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 959 1028 1131
Size: 959 69 103
Fragments arranged by size:
959 103 69
EcoRI G'AATT_C
Cuts at: 0 471 1131
Size: 471 660
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 799 831 1131
Size: 799 32 300
Fragments arranged by size:
799 300 32
HincII GTy'rAC
Cuts at: 0 303 435 1131
Size: 303 132 696
Fragments arranged by size:
696 303 132
HindIII A'AGCT_T
Cuts at: 0 217 1131
Size: 217 914
HpaI GTT'AAC
Cuts at: 0 435 1131
Size: 435 696
MscI TGG'CCA
Cuts at: 0 1055 1131
Size: 1055 76
MspA1I CmG'CkG
Cuts at: 0 15 499 568 637 706 775 844 913
Size: 15 484 69 69 69 69 69 69
Cuts at: 913 979 1131
Size: 66 152
Fragments arranged by size:
484 152 69 69 69 69 69 69
66 15
NheI G'CTAG_C
Cuts at: 0 153 1131
Size: 153 978
PfoI T'CCnGG_A
Cuts at: 0 144 1131
Size: 144 987
PvuII CAG'CTG
Cuts at: 0 499 568 637 706 775 844 913 979
Size: 499 69 69 69 69 69 69 66
Cuts at: 979 1131
Size: 152
Fragments arranged by size:
499 152 69 69 69 69 69 69
66
SapI GCTCTTCn'nnn_
Cuts at: 0 450 1131
Size: 450 681
SfcI C'TryA_G
Cuts at: 0 207 449 1131
Size: 207 242 682
Fragments arranged by size:
682 242 207
StuI AGG'CCT
Cuts at: 0 213 1131
Size: 213 918
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 129 522 591 660 729 798 867 996
Size: 129 393 69 69 69 69 69 129
Cuts at: 996 999 1131
Size: 3 132
Fragments arranged by size:
393 132 129 129 69 69 69 69
69 3
XmnI GAAnn'nnTTC
Cuts at: 0 169 1131
Size: 169 962
Enzymes that do cut:
AcuI AlwNI ApaLI ApoI BbsI BbvCI Bme1580I BmtI
Bpu10I BsaI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BstAPI BtsI DraIII EaeI EarI EciI Eco57MI
EcoRI Hin4I HincII HindIII HpaI MscI MspA1I NheI
PfoI PvuII SapI SfcI StuI TaqII XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmrI BplI BpmI BpuEI BsaAI BsaBI BsaHI BsaWI
BseRI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI
EagI EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI
FspAI HaeII KasI KpnI MfeI MluI MmeI MslI
NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StyI SwaI TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI