Restriction map for
pFN21AB8973
(Linear) (Six-base) MAPSORT of: KIBB8973.seq Check: 6270 from: 1 to: 1101
KIBB8973.orf
With 182 enzymes: SgfI *
December 27, 2011 16:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 460 721 1101
Size: 460 261 380
Fragments arranged by size:
460 380 261
AccI GT'mk_AC
Cuts at: 0 350 854 1101
Size: 350 504 247
Fragments arranged by size:
504 350 247
AflIII A'CryG_T
Cuts at: 0 284 425 1101
Size: 284 141 676
Fragments arranged by size:
676 284 141
AhdI GACnn_n'nnGTC
Cuts at: 0 264 1101
Size: 264 837
AlwNI CAG_nnn'CTG
Cuts at: 0 67 750 1101
Size: 67 683 351
Fragments arranged by size:
683 351 67
ApaI G_GGCC'C
Cuts at: 0 1042 1101
Size: 1042 59
ApaLI G'TGCA_C
Cuts at: 0 484 1101
Size: 484 617
BamHI G'GATC_C
Cuts at: 0 25 1101
Size: 25 1076
BanII G_rGCy'C
Cuts at: 0 592 1042 1078 1101
Size: 592 450 36 23
Fragments arranged by size:
592 450 36 23
BclI T'GATC_A
Cuts at: 0 116 725 1101
Size: 116 609 376
Fragments arranged by size:
609 376 116
BfrBI ATG'CAT
Cuts at: 0 297 1101
Size: 297 804
Bme1580I G_kGCm'C
Cuts at: 0 488 833 957 1042 1101
Size: 488 345 124 85 59
Fragments arranged by size:
488 345 124 85 59
BmgBI CAC'GTC
Cuts at: 0 840 1101
Size: 840 261
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 666 1101
Size: 666 435
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 190 1101
Size: 57 133 911
Fragments arranged by size:
911 133 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 754 1018 1101
Size: 487 267 264 83
Fragments arranged by size:
487 267 264 83
BsaI GGTCTCn'nnnn_
Cuts at: 0 251 1101
Size: 251 850
BsaAI yAC'GTr
Cuts at: 0 858 1101
Size: 858 243
BsaHI Gr'CG_yC
Cuts at: 0 163 1101
Size: 163 938
BseYI C'CCAG_C
Cuts at: 0 896 1048 1101
Size: 896 152 53
Fragments arranged by size:
896 152 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 847 1101
Size: 622 225 254
Fragments arranged by size:
622 254 225
BsiEI CG_ry'CG
Cuts at: 0 468 1101
Size: 468 633
BsiHKAI G_wGCw'C
Cuts at: 0 488 1078 1101
Size: 488 590 23
Fragments arranged by size:
590 488 23
BsmBI CGTCTCn'nnnn_
Cuts at: 0 154 403 1101
Size: 154 249 698
Fragments arranged by size:
698 249 154
Bsp1286I G_dGCh'C
Cuts at: 0 488 592 833 957 1042 1078 1101
Size: 488 104 241 124 85 36 23
Fragments arranged by size:
488 241 124 104 85 36 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 460 721 728 1101
Size: 460 261 7 373
Fragments arranged by size:
460 373 261 7
BsrBI CCG'CTC
Cuts at: 0 848 1101
Size: 848 253
BsrDI GCAATG_nn'
Cuts at: 0 154 1101
Size: 154 947
BsrFI r'CCGG_y
Cuts at: 0 403 1101
Size: 403 698
BstAPI GCAn_nnn'nTGC
Cuts at: 0 750 1101
Size: 750 351
BstBI TT'CG_AA
Cuts at: 0 1055 1101
Size: 1055 46
BstEII G'GTnAC_C
Cuts at: 0 708 1101
Size: 708 393
BstXI CCAn_nnnn'nTGG
Cuts at: 0 345 361 804 816 1101
Size: 345 16 443 12 285
Fragments arranged by size:
443 345 285 16 12
BstYI r'GATC_y
Cuts at: 0 25 1101
Size: 25 1076
BtgI C'CryG_G
Cuts at: 0 137 305 569 704 876 1101
Size: 137 168 264 135 172 225
Fragments arranged by size:
264 225 172 168 137 135
BtsI GCAGTG_nn'
Cuts at: 0 683 788 1101
Size: 683 105 313
Fragments arranged by size:
683 313 105
DraIII CAC_nnn'GTG
Cuts at: 0 618 1101
Size: 618 483
EaeI y'GGCC_r
Cuts at: 0 134 153 278 302 308 373 476 566
Size: 134 19 125 24 6 65 103 90
Cuts at: 566 806 1101
Size: 240 295
Fragments arranged by size:
295 240 134 125 103 90 65 24
19 6
EarI CTCTTCn'nnn_
Cuts at: 0 418 1101
Size: 418 683
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 985 1101
Size: 985 116
EcoICRI GAG'CTC
Cuts at: 0 1076 1101
Size: 1076 25
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 190 1101
Size: 57 133 911
Fragments arranged by size:
911 133 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 742 1101
Size: 742 359
EcoO109I rG'GnC_Cy
Cuts at: 0 272 515 747 881 1038 1101
Size: 272 243 232 134 157 63
Fragments arranged by size:
272 243 232 157 134 63
FspI TGC'GCA
Cuts at: 0 787 1101
Size: 787 314
FspAI rTGC'GCAy
Cuts at: 0 787 1101
Size: 787 314
HaeII r_GCGC'y
Cuts at: 0 464 768 1101
Size: 464 304 333
Fragments arranged by size:
464 333 304
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 250 282 1101
Size: 250 32 819
Fragments arranged by size:
819 250 32
HincII GTy'rAC
Cuts at: 0 843 1101
Size: 843 258
MscI TGG'CCA
Cuts at: 0 136 155 375 478 568 808 1101
Size: 136 19 220 103 90 240 293
Fragments arranged by size:
293 240 220 136 103 90 19
MspA1I CmG'CkG
Cuts at: 0 579 633 1101
Size: 579 54 468
Fragments arranged by size:
579 468 54
NcoI C'CATG_G
Cuts at: 0 569 876 1101
Size: 569 307 225
Fragments arranged by size:
569 307 225
NsiI A_TGCA'T
Cuts at: 0 299 1101
Size: 299 802
NspI r_CATG'y
Cuts at: 0 180 395 429 1101
Size: 180 215 34 672
Fragments arranged by size:
672 215 180 34
PciI A'CATG_T
Cuts at: 0 425 1101
Size: 425 676
PflMI CCAn_nnn'nTGG
Cuts at: 0 618 663 1101
Size: 618 45 438
Fragments arranged by size:
618 438 45
PfoI T'CCnGG_A
Cuts at: 0 268 1101
Size: 268 833
PpuMI rG'GwC_Cy
Cuts at: 0 272 1101
Size: 272 829
PspOMI G'GGCC_C
Cuts at: 0 1038 1101
Size: 1038 63
PstI C_TGCA'G
Cuts at: 0 632 1101
Size: 632 469
PvuII CAG'CTG
Cuts at: 0 633 1101
Size: 633 468
SacI G_AGCT'C
Cuts at: 0 1078 1101
Size: 1078 23
SanDI GG'GwC_CC
Cuts at: 0 272 1101
Size: 272 829
SfcI C'TryA_G
Cuts at: 0 628 1101
Size: 628 473
SmlI C'TyrA_G
Cuts at: 0 502 769 1033 1101
Size: 502 267 264 68
Fragments arranged by size:
502 267 264 68
SphI G_CATG'C
Cuts at: 0 395 1101
Size: 395 706
StuI AGG'CCT
Cuts at: 0 649 1101
Size: 649 452
StyI C'CwwG_G
Cuts at: 0 569 776 876 1101
Size: 569 207 100 225
Fragments arranged by size:
569 225 207 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 540 910 1101
Size: 540 370 191
Fragments arranged by size:
540 370 191
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 85 129 376 615 1101
Size: 85 44 247 239 486
Fragments arranged by size:
486 247 239 85 44
Enzymes that do cut:
AarI AccI AflIII AhdI AlwNI ApaI ApaLI BamHI
BanII BclI BfrBI Bme1580I BmgBI BplI BpmI BpuEI
BsaI BsaAI BsaHI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstBI BstEII
BstXI BstYI BtgI BtsI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI HaeII Hin4I
HincII MscI MspA1I NcoI NsiI NspI PciI PflMI
PfoI PpuMI PspOMI PstI PvuII SacI SanDI SfcI
SmlI SphI StuI StyI TaqII XcmI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AleI
AlfI AloI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BanI BbeI BbsI BbvCI BcgI BciVI BglI
BglII BlpI BmrI BmtI Bpu10I BsaBI BsaWI BsaXI
BseRI BsiWI BsmI BspEI BspHI BsrGI BssHII BssSI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI
EcoRI EcoRV FalI FseI HindIII HpaI KasI KpnI
MfeI MluI MmeI MslI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PasI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI