Restriction map for
pFN21AB8968
(Linear) (Six-base) MAPSORT of: KIBB8968.seq Check: 571 from: 1 to: 1071
KIBB8968.orf
With 182 enzymes: SgfI *
December 5, 2014 12:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 249 1071
Size: 249 822
AccI GT'mk_AC
Cuts at: 0 1022 1071
Size: 1022 49
Acc65I G'GTAC_C
Cuts at: 0 350 1071
Size: 350 721
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 1071
Size: 416 655
AfeI AGC'GCT
Cuts at: 0 334 748 1071
Size: 334 414 323
Fragments arranged by size:
414 334 323
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 323 1071
Size: 323 748
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 73 105 1071
Size: 73 32 966
Fragments arranged by size:
966 73 32
AlwNI CAG_nnn'CTG
Cuts at: 0 999 1071
Size: 999 72
ApaI G_GGCC'C
Cuts at: 0 228 978 1071
Size: 228 750 93
Fragments arranged by size:
750 228 93
AvaI C'yCGr_G
Cuts at: 0 10 724 1071
Size: 10 714 347
Fragments arranged by size:
714 347 10
BanI G'GyrC_C
Cuts at: 0 28 235 350 573 915 981 1071
Size: 28 207 115 223 342 66 90
Fragments arranged by size:
342 223 207 115 90 66 28
BanII G_rGCy'C
Cuts at: 0 228 514 978 1071
Size: 228 286 464 93
Fragments arranged by size:
464 286 228 93
BbeI G_GCGC'C
Cuts at: 0 239 577 1071
Size: 239 338 494
Fragments arranged by size:
494 338 239
BbsI GAAGACnn'nnnn_
Cuts at: 0 635 690 691 708 1071
Size: 635 55 1 17 363
Fragments arranged by size:
635 363 55 17 1
BbvCI CC'TCA_GC
Cuts at: 0 143 1071
Size: 143 928
BglI GCCn_nnn'nGGC
Cuts at: 0 214 223 778 973 1071
Size: 214 9 555 195 98
Fragments arranged by size:
555 214 195 98 9
BglII A'GATC_T
Cuts at: 0 815 1071
Size: 815 256
Bme1580I G_kGCm'C
Cuts at: 0 228 920 978 1071
Size: 228 692 58 93
Fragments arranged by size:
692 228 93 58
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 519 558 1071
Size: 519 39 513
Fragments arranged by size:
519 513 39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 349 550 889 1071
Size: 250 99 201 339 182
Fragments arranged by size:
339 250 201 182 99
Bpu10I CC'TnA_GC
Cuts at: 0 137 143 883 1071
Size: 137 6 740 188
Fragments arranged by size:
740 188 137 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 1071
Size: 457 614
BsaI GGTCTCn'nnnn_
Cuts at: 0 110 1047 1071
Size: 110 937 24
Fragments arranged by size:
937 110 24
BsaHI Gr'CG_yC
Cuts at: 0 236 574 1071
Size: 236 338 497
Fragments arranged by size:
497 338 236
BsaWI w'CCGG_w
Cuts at: 0 459 1071
Size: 459 612
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 73 103 1071
Size: 73 30 968
Fragments arranged by size:
968 73 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 417 547 1071
Size: 417 130 524
Fragments arranged by size:
524 417 130
BseYI C'CCAG_C
Cuts at: 0 339 1071
Size: 339 732
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 1071
Size: 775 296
BsiEI CG_ry'CG
Cuts at: 0 130 587 947 1066 1071
Size: 130 457 360 119 5
Fragments arranged by size:
457 360 130 119 5
BsmBI CGTCTCn'nnnn_
Cuts at: 0 796 1071
Size: 796 275
Bsp1286I G_dGCh'C
Cuts at: 0 228 514 920 978 1071
Size: 228 286 406 58 93
Fragments arranged by size:
406 286 228 93 58
BspEI T'CCGG_A
Cuts at: 0 459 1071
Size: 459 612
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 113 249 798 1071
Size: 113 136 549 273
Fragments arranged by size:
549 273 136 113
BsrBI CCG'CTC
Cuts at: 0 73 1071
Size: 73 998
BsrFI r'CCGG_y
Cuts at: 0 25 125 373 666 772 1065 1071
Size: 25 100 248 293 106 293 6
Fragments arranged by size:
293 293 248 106 100 25 6
BssSI C'ACGA_G
Cuts at: 0 931 1071
Size: 931 140
BstXI CCAn_nnnn'nTGG
Cuts at: 0 347 1071
Size: 347 724
BstYI r'GATC_y
Cuts at: 0 290 815 1071
Size: 290 525 256
Fragments arranged by size:
525 290 256
BtgI C'CryG_G
Cuts at: 0 154 209 239 893 968 1071
Size: 154 55 30 654 75 103
Fragments arranged by size:
654 154 103 75 55 30
BtsI GCAGTG_nn'
Cuts at: 0 466 1071
Size: 466 605
DraIII CAC_nnn'GTG
Cuts at: 0 1038 1071
Size: 1038 33
EaeI y'GGCC_r
Cuts at: 0 127 215 378 584 611 779 875 944
Size: 127 88 163 206 27 168 96 69
Cuts at: 944 1063 1071
Size: 119 8
Fragments arranged by size:
206 168 163 127 119 96 88 69
27 8
EagI C'GGCC_G
Cuts at: 0 127 584 944 1063 1071
Size: 127 457 360 119 8
Fragments arranged by size:
457 360 127 119 8
EarI CTCTTCn'nnn_
Cuts at: 0 169 1050 1071
Size: 169 881 21
Fragments arranged by size:
881 169 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 877 1071
Size: 877 194
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 349 416 550 889 1071
Size: 250 99 67 134 339 182
Fragments arranged by size:
339 250 182 134 99 67
EcoO109I rG'GnC_Cy
Cuts at: 0 224 225 974 975 1071
Size: 224 1 749 1 96
Fragments arranged by size:
749 224 96 1 1
HaeII r_GCGC'y
Cuts at: 0 239 336 577 647 750 1071
Size: 239 97 241 70 103 321
Fragments arranged by size:
321 241 239 103 97 70
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 439 471 1071
Size: 439 32 600
Fragments arranged by size:
600 439 32
KasI G'GCGC_C
Cuts at: 0 235 573 1071
Size: 235 338 498
Fragments arranged by size:
498 338 235
KpnI G_GTAC'C
Cuts at: 0 354 1071
Size: 354 717
MspA1I CmG'CkG
Cuts at: 0 199 205 211 241 855 895 970 999
Size: 199 6 6 30 614 40 75 29
Cuts at: 999 1056 1071
Size: 57 15
Fragments arranged by size:
614 199 75 57 40 30 29 15
6 6
NaeI GCC'GGC
Cuts at: 0 27 375 668 774 1071
Size: 27 348 293 106 297
Fragments arranged by size:
348 297 293 106 27
NarI GG'CG_CC
Cuts at: 0 236 574 1071
Size: 236 338 497
Fragments arranged by size:
497 338 236
NgoMIV G'CCGG_C
Cuts at: 0 25 373 666 772 1071
Size: 25 348 293 106 299
Fragments arranged by size:
348 299 293 106 25
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 73 105 1071
Size: 73 32 966
Fragments arranged by size:
966 73 32
PshAI GACnn'nnGTC
Cuts at: 0 808 1071
Size: 808 263
PspOMI G'GGCC_C
Cuts at: 0 224 974 1071
Size: 224 750 97
Fragments arranged by size:
750 224 97
PspXI vC'TCGA_Gb
Cuts at: 0 724 1071
Size: 724 347
PstI C_TGCA'G
Cuts at: 0 966 1071
Size: 966 105
PvuII CAG'CTG
Cuts at: 0 855 1071
Size: 855 216
SacII CC_GC'GG
Cuts at: 0 212 242 896 971 1071
Size: 212 30 654 75 100
Fragments arranged by size:
654 212 100 75 30
SapI GCTCTTCn'nnn_
Cuts at: 0 169 1071
Size: 169 902
SfcI C'TryA_G
Cuts at: 0 962 1071
Size: 962 109
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 223 1071
Size: 223 848
SfoI GGC'GCC
Cuts at: 0 237 575 1071
Size: 237 338 496
Fragments arranged by size:
496 338 237
SgrAI Cr'CCGG_yG
Cuts at: 0 373 1071
Size: 373 698
SmaI CCC'GGG
Cuts at: 0 12 1071
Size: 12 1059
SmlI C'TyrA_G
Cuts at: 0 472 724 1071
Size: 472 252 347
Fragments arranged by size:
472 347 252
StuI AGG'CCT
Cuts at: 0 412 1071
Size: 412 659
StyI C'CwwG_G
Cuts at: 0 77 218 1071
Size: 77 141 853
Fragments arranged by size:
853 141 77
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 225 1071
Size: 225 846
XhoI C'TCGA_G
Cuts at: 0 724 1071
Size: 724 347
XmaI C'CCGG_G
Cuts at: 0 10 1071
Size: 10 1061
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AlfI AloI AlwNI
ApaI AvaI BanI BanII BbeI BbsI BbvCI BglI
BglII Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrFI BssSI BstXI BstYI BtgI
BtsI DraIII EaeI EagI EarI EciI Eco57MI EcoO109I
HaeII Hin4I KasI KpnI MspA1I NaeI NarI NgoMIV
PpiI PshAI PspOMI PspXI PstI PvuII SacII SapI
SfcI SfiI SfoI SgrAI SmaI SmlI StuI StyI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AhdI AleI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BciVI BclI BfrBI BlpI BmgBI BmrI BmtI BsaAI
BsaBI BsiHKAI BsiWI BsmI BspHI BsrDI BsrGI BssHII
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI MfeI MluI MmeI
MscI MslI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpuMI PsiI PsrI PvuI RsrII SacI SalI SanDI
SbfI ScaI SexAI SgfI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TatI TstI Tth111I XbaI XmnI
ZraI