Restriction map for
pFN21AB8957
(Linear) (Six-base) MAPSORT of: KIBB8957.seq Check: 3829 from: 1 to: 999
KIBB8957.orf
With 182 enzymes: SgfI *
December 5, 2014 12:54 ..
AccI GT'mk_AC
Cuts at: 0 650 999
Size: 650 349
Acc65I G'GTAC_C
Cuts at: 0 800 999
Size: 800 199
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 999
Size: 665 334
AlwNI CAG_nnn'CTG
Cuts at: 0 828 840 999
Size: 828 12 159
Fragments arranged by size:
828 159 12
ApaLI G'TGCA_C
Cuts at: 0 931 999
Size: 931 68
AvaI C'yCGr_G
Cuts at: 0 84 108 229 369 377 999
Size: 84 24 121 140 8 622
Fragments arranged by size:
622 140 121 84 24 8
BanI G'GyrC_C
Cuts at: 0 128 165 496 607 800 818 999
Size: 128 37 331 111 193 18 181
Fragments arranged by size:
331 193 181 128 111 37 18
BanII G_rGCy'C
Cuts at: 0 141 370 376 386 583 999
Size: 141 229 6 10 197 416
Fragments arranged by size:
416 229 197 141 10 6
BbeI G_GCGC'C
Cuts at: 0 132 169 500 611 822 999
Size: 132 37 331 111 211 177
Fragments arranged by size:
331 211 177 132 111 37
BglI GCCn_nnn'nGGC
Cuts at: 0 77 999
Size: 77 922
BlpI GC'TnA_GC
Cuts at: 0 841 999
Size: 841 158
Bme1580I G_kGCm'C
Cuts at: 0 935 999
Size: 935 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 760 999
Size: 526 234 239
Fragments arranged by size:
526 239 234
BsaI GGTCTCn'nnnn_
Cuts at: 0 698 999
Size: 698 301
BsaHI Gr'CG_yC
Cuts at: 0 129 166 497 608 819 999
Size: 129 37 331 111 211 180
Fragments arranged by size:
331 211 180 129 111 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 946 970 976 999
Size: 946 24 6 23
Fragments arranged by size:
946 24 23 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 293 999
Size: 19 274 706
Fragments arranged by size:
706 274 19
BseYI C'CCAG_C
Cuts at: 0 429 999
Size: 429 570
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 999
Size: 472 527
BsiEI CG_ry'CG
Cuts at: 0 240 739 999
Size: 240 499 260
Fragments arranged by size:
499 260 240
BsiHKAI G_wGCw'C
Cuts at: 0 583 935 999
Size: 583 352 64
Fragments arranged by size:
583 352 64
Bsp1286I G_dGCh'C
Cuts at: 0 141 370 376 386 583 935 999
Size: 141 229 6 10 197 352 64
Fragments arranged by size:
352 229 197 141 64 10 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 268 999
Size: 268 731
BsrBI CCG'CTC
Cuts at: 0 320 426 999
Size: 320 106 573
Fragments arranged by size:
573 320 106
BsrFI r'CCGG_y
Cuts at: 0 131 239 298 436 604 639 671 999
Size: 131 108 59 138 168 35 32 328
Fragments arranged by size:
328 168 138 131 108 59 35 32
BsrGI T'GTAC_A
Cuts at: 0 953 999
Size: 953 46
BssHII G'CGCG_C
Cuts at: 0 620 885 999
Size: 620 265 114
Fragments arranged by size:
620 265 114
BstAPI GCAn_nnn'nTGC
Cuts at: 0 630 999
Size: 630 369
BtgI C'CryG_G
Cuts at: 0 152 549 905 999
Size: 152 397 356 94
Fragments arranged by size:
397 356 152 94
EaeI y'GGCC_r
Cuts at: 0 237 637 736 926 999
Size: 237 400 99 190 73
Fragments arranged by size:
400 237 190 99 73
EagI C'GGCC_G
Cuts at: 0 237 736 999
Size: 237 499 263
Fragments arranged by size:
499 263 237
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 308 999
Size: 308 691
EcoICRI GAG'CTC
Cuts at: 0 581 999
Size: 581 418
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 999
Size: 665 334
EcoNI CCTnn'n_nnAGG
Cuts at: 0 680 832 999
Size: 680 152 167
Fragments arranged by size:
680 167 152
EcoO109I rG'GnC_Cy
Cuts at: 0 257 685 999
Size: 257 428 314
Fragments arranged by size:
428 314 257
HaeII r_GCGC'y
Cuts at: 0 132 169 500 611 822 999
Size: 132 37 331 111 211 177
Fragments arranged by size:
331 211 177 132 111 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 970 999
Size: 970 29
KasI G'GCGC_C
Cuts at: 0 128 165 496 607 818 999
Size: 128 37 331 111 211 181
Fragments arranged by size:
331 211 181 128 111 37
KpnI G_GTAC'C
Cuts at: 0 804 999
Size: 804 195
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 968 999
Size: 968 31
MspA1I CmG'CkG
Cuts at: 0 34 54 154 551 765 816 999
Size: 34 20 100 397 214 51 183
Fragments arranged by size:
397 214 183 100 51 34 20
NaeI GCC'GGC
Cuts at: 0 133 241 300 438 606 641 999
Size: 133 108 59 138 168 35 358
Fragments arranged by size:
358 168 138 133 108 59 35
NarI GG'CG_CC
Cuts at: 0 129 166 497 608 819 999
Size: 129 37 331 111 211 180
Fragments arranged by size:
331 211 180 129 111 37
NgoMIV G'CCGG_C
Cuts at: 0 131 239 298 436 604 639 999
Size: 131 108 59 138 168 35 360
Fragments arranged by size:
360 168 138 131 108 59 35
NotI GC'GGCC_GC
Cuts at: 0 736 999
Size: 736 263
PflMI CCAn_nnn'nTGG
Cuts at: 0 635 828 999
Size: 635 193 171
Fragments arranged by size:
635 193 171
PpuMI rG'GwC_Cy
Cuts at: 0 257 685 999
Size: 257 428 314
Fragments arranged by size:
428 314 257
PstI C_TGCA'G
Cuts at: 0 164 770 999
Size: 164 606 229
Fragments arranged by size:
606 229 164
PvuII CAG'CTG
Cuts at: 0 765 999
Size: 765 234
RsrII CG'GwC_CG
Cuts at: 0 669 999
Size: 669 330
SacI G_AGCT'C
Cuts at: 0 583 999
Size: 583 416
SacII CC_GC'GG
Cuts at: 0 155 552 999
Size: 155 397 447
Fragments arranged by size:
447 397 155
SbfI CC_TGCA'GG
Cuts at: 0 164 999
Size: 164 835
SfcI C'TryA_G
Cuts at: 0 160 766 999
Size: 160 606 233
Fragments arranged by size:
606 233 160
SfoI GGC'GCC
Cuts at: 0 130 167 498 609 820 999
Size: 130 37 331 111 211 179
Fragments arranged by size:
331 211 179 130 111 37
SgrAI Cr'CCGG_yG
Cuts at: 0 131 999
Size: 131 868
SmaI CCC'GGG
Cuts at: 0 110 999
Size: 110 889
SmlI C'TyrA_G
Cuts at: 0 541 775 999
Size: 541 234 224
Fragments arranged by size:
541 234 224
SrfI GCCC'GGGC
Cuts at: 0 110 999
Size: 110 889
StyI C'CwwG_G
Cuts at: 0 974 999
Size: 974 25
TatI w'GTAC_w
Cuts at: 0 953 999
Size: 953 46
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 249 281 999
Size: 249 32 718
Fragments arranged by size:
718 249 32
XmaI C'CCGG_G
Cuts at: 0 108 999
Size: 108 891
Enzymes that do cut:
AccI Acc65I AcuI AlwNI ApaLI AvaI BanI BanII
BbeI BglI BlpI Bme1580I BpuEI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BsrGI BssHII BstAPI BtgI EaeI EagI EciI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI KpnI
MmeI MspA1I NaeI NarI NgoMIV NotI PflMI PpuMI
PstI PvuII RsrII SacI SacII SbfI SfcI SfoI
SgrAI SmaI SmlI SrfI StyI TatI TstI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BmgBI BmrI BmtI BplI BpmI Bpu10I
BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI MfeI MluI MscI MslI NcoI NdeI NheI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
SalI SanDI SapI ScaI SexAI SfiI SgfI SnaBI
SpeI SphI SspI StuI SwaI TaqII Tth111I XbaI
XcmI XhoI XmnI ZraI