Restriction map for
pFN21AB8949
(Linear) (Six-base) MAPSORT of: KIBB8949.seq Check: 1672 from: 1 to: 972
KIBB8949.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AccI GT'mk_AC
Cuts at: 0 647 972
Size: 647 325
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 972
Size: 577 395
AfeI AGC'GCT
Cuts at: 0 30 972
Size: 30 942
AflIII A'CryG_T
Cuts at: 0 961 972
Size: 961 11
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 752 972
Size: 752 220
AlwNI CAG_nnn'CTG
Cuts at: 0 643 884 972
Size: 643 241 88
Fragments arranged by size:
643 241 88
ApaI G_GGCC'C
Cuts at: 0 127 172 972
Size: 127 45 800
Fragments arranged by size:
800 127 45
AvaI C'yCGr_G
Cuts at: 0 165 972
Size: 165 807
BanI G'GyrC_C
Cuts at: 0 141 290 346 757 972
Size: 141 149 56 411 215
Fragments arranged by size:
411 215 149 141 56
BanII G_rGCy'C
Cuts at: 0 127 172 972
Size: 127 45 800
Fragments arranged by size:
800 127 45
BbeI G_GCGC'C
Cuts at: 0 350 972
Size: 350 622
BbsI GAAGACnn'nnnn_
Cuts at: 0 479 608 972
Size: 479 129 364
Fragments arranged by size:
479 364 129
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 763 797 972
Size: 763 34 175
Fragments arranged by size:
763 175 34
BglI GCCn_nnn'nGGC
Cuts at: 0 154 176 972
Size: 154 22 796
Fragments arranged by size:
796 154 22
Bme1580I G_kGCm'C
Cuts at: 0 127 146 172 293 972
Size: 127 19 26 121 679
Fragments arranged by size:
679 127 121 26 19
BmgBI CAC'GTC
Cuts at: 0 338 972
Size: 338 634
BmrI ACTGGGnnnn_n'
Cuts at: 0 660 972
Size: 660 312
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 240 972
Size: 240 732
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 814 972
Size: 718 96 158
Fragments arranged by size:
718 158 96
BsaHI Gr'CG_yC
Cuts at: 0 347 355 972
Size: 347 8 617
Fragments arranged by size:
617 347 8
BsaWI w'CCGG_w
Cuts at: 0 240 972
Size: 240 732
BseYI C'CCAG_C
Cuts at: 0 25 68 358 972
Size: 25 43 290 614
Fragments arranged by size:
614 290 43 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 430 972
Size: 360 70 542
Fragments arranged by size:
542 360 70
BsiEI CG_ry'CG
Cuts at: 0 101 972
Size: 101 871
Bsp1286I G_dGCh'C
Cuts at: 0 127 146 172 293 972
Size: 127 19 26 121 679
Fragments arranged by size:
679 127 121 26 19
BspHI T'CATG_A
Cuts at: 0 849 872 972
Size: 849 23 100
Fragments arranged by size:
849 100 23
BsrFI r'CCGG_y
Cuts at: 0 19 190 809 972
Size: 19 171 619 163
Fragments arranged by size:
619 171 163 19
BssSI C'ACGA_G
Cuts at: 0 214 972
Size: 214 758
BtgI C'CryG_G
Cuts at: 0 137 149 425 972
Size: 137 12 276 547
Fragments arranged by size:
547 276 137 12
BtsI GCAGTG_nn'
Cuts at: 0 283 972
Size: 283 689
DraIII CAC_nnn'GTG
Cuts at: 0 297 972
Size: 297 675
EaeI y'GGCC_r
Cuts at: 0 98 675 866 972
Size: 98 577 191 106
Fragments arranged by size:
577 191 106 98
EagI C'GGCC_G
Cuts at: 0 98 972
Size: 98 874
EarI CTCTTCn'nnn_
Cuts at: 0 235 972
Size: 235 737
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 828 972
Size: 828 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 577 718 814 972
Size: 577 141 96 158
Fragments arranged by size:
577 158 141 96
EcoO109I rG'GnC_Cy
Cuts at: 0 124 169 536 972
Size: 124 45 367 436
Fragments arranged by size:
436 367 124 45
FseI GG_CCGG'CC
Cuts at: 0 194 972
Size: 194 778
HaeII r_GCGC'y
Cuts at: 0 32 350 972
Size: 32 318 622
Fragments arranged by size:
622 318 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 543 575 590 622 972
Size: 543 32 15 32 350
Fragments arranged by size:
543 350 32 32 15
HincII GTy'rAC
Cuts at: 0 648 972
Size: 648 324
KasI G'GCGC_C
Cuts at: 0 346 972
Size: 346 626
MscI TGG'CCA
Cuts at: 0 677 972
Size: 677 295
MslI CAynn'nnrTG
Cuts at: 0 691 854 972
Size: 691 163 118
Fragments arranged by size:
691 163 118
MspA1I CmG'CkG
Cuts at: 0 139 151 972
Size: 139 12 821
Fragments arranged by size:
821 139 12
NaeI GCC'GGC
Cuts at: 0 192 972
Size: 192 780
NarI GG'CG_CC
Cuts at: 0 347 972
Size: 347 625
NdeI CA'TA_TG
Cuts at: 0 478 972
Size: 478 494
NgoMIV G'CCGG_C
Cuts at: 0 190 972
Size: 190 782
NspI r_CATG'y
Cuts at: 0 965 972
Size: 965 7
PciI A'CATG_T
Cuts at: 0 961 972
Size: 961 11
PflMI CCAn_nnn'nTGG
Cuts at: 0 511 972
Size: 511 461
PpuMI rG'GwC_Cy
Cuts at: 0 536 972
Size: 536 436
PspOMI G'GGCC_C
Cuts at: 0 123 168 972
Size: 123 45 804
Fragments arranged by size:
804 123 45
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 769 801 972
Size: 769 32 171
Fragments arranged by size:
769 171 32
PstI C_TGCA'G
Cuts at: 0 46 278 972
Size: 46 232 694
Fragments arranged by size:
694 232 46
SacII CC_GC'GG
Cuts at: 0 140 152 972
Size: 140 12 820
Fragments arranged by size:
820 140 12
SalI G'TCGA_C
Cuts at: 0 646 972
Size: 646 326
SanDI GG'GwC_CC
Cuts at: 0 536 972
Size: 536 436
SapI GCTCTTCn'nnn_
Cuts at: 0 235 972
Size: 235 737
SexAI A'CCwGG_T
Cuts at: 0 293 972
Size: 293 679
SfcI C'TryA_G
Cuts at: 0 42 274 620 786 972
Size: 42 232 346 166 186
Fragments arranged by size:
346 232 186 166 42
SfoI GGC'GCC
Cuts at: 0 348 972
Size: 348 624
StyI C'CwwG_G
Cuts at: 0 936 972
Size: 936 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 642 972
Size: 642 330
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 64 96 972
Size: 64 32 876
Fragments arranged by size:
876 64 32
Tth111I GACn'n_nGTC
Cuts at: 0 256 607 972
Size: 256 351 365
Fragments arranged by size:
365 351 256
Enzymes that do cut:
AccI AcuI AfeI AflIII AlfI AlwNI ApaI AvaI
BanI BanII BbeI BbsI BcgI BglI Bme1580I BmgBI
BmrI BplI BpmI BsaHI BsaWI BseYI BsgI BsiEI
Bsp1286I BspHI BsrFI BssSI BtgI BtsI DraIII EaeI
EagI EarI EciI Eco57MI EcoO109I FseI HaeII Hin4I
HincII KasI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PciI PflMI PpuMI PspOMI PsrI PstI
SacII SalI SanDI SapI SexAI SfcI SfoI StyI
TaqII TstI Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BciVI BclI BfrBI BglII BlpI BmtI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI BsiHKAI
BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DrdI EcoICRI EcoNI EcoRI
EcoRV FalI FspI FspAI HindIII HpaI KpnI MfeI
MluI MmeI NcoI NheI NotI NruI NsiI PacI
PasI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PvuI PvuII RsrII SacI SbfI ScaI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI XbaI XcmI XhoI XmaI XmnI
ZraI