Restriction map for
pFN21AB8948
(Linear) (Six-base) MAPSORT of: KIBB8948.seq Check: 6028 from: 1 to: 948
KIBB8948.orf
With 182 enzymes: SgfI *
August 14, 2012 18:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 276 948
Size: 276 672
AatII G_ACGT'C
Cuts at: 0 747 948
Size: 747 201
AflIII A'CryG_T
Cuts at: 0 8 948
Size: 8 940
AlwNI CAG_nnn'CTG
Cuts at: 0 86 850 948
Size: 86 764 98
Fragments arranged by size:
764 98 86
ApaI G_GGCC'C
Cuts at: 0 220 948
Size: 220 728
AvaI C'yCGr_G
Cuts at: 0 355 385 729 948
Size: 355 30 344 219
Fragments arranged by size:
355 344 219 30
BanI G'GyrC_C
Cuts at: 0 258 312 393 654 757 948
Size: 258 54 81 261 103 191
Fragments arranged by size:
261 258 191 103 81 54
BanII G_rGCy'C
Cuts at: 0 220 916 948
Size: 220 696 32
Fragments arranged by size:
696 220 32
BbeI G_GCGC'C
Cuts at: 0 316 397 658 948
Size: 316 81 261 290
Fragments arranged by size:
316 290 261 81
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 81 115 948
Size: 81 34 833
Fragments arranged by size:
833 81 34
BglI GCCn_nnn'nGGC
Cuts at: 0 148 948
Size: 148 800
BlpI GC'TnA_GC
Cuts at: 0 124 948
Size: 124 824
Bme1580I G_kGCm'C
Cuts at: 0 220 263 948
Size: 220 43 685
Fragments arranged by size:
685 220 43
BmrI ACTGGGnnnn_n'
Cuts at: 0 58 442 948
Size: 58 384 506
Fragments arranged by size:
506 384 58
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 563 948
Size: 449 114 385
Fragments arranged by size:
449 385 114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 644 948
Size: 644 304
BsaHI Gr'CG_yC
Cuts at: 0 225 313 394 433 655 744 948
Size: 225 88 81 39 222 89 204
Fragments arranged by size:
225 222 204 89 88 81 39
BsaWI w'CCGG_w
Cuts at: 0 489 501 513 948
Size: 489 12 12 435
Fragments arranged by size:
489 435 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 133 403 948
Size: 133 270 545
Fragments arranged by size:
545 270 133
BseYI C'CCAG_C
Cuts at: 0 179 597 948
Size: 179 418 351
Fragments arranged by size:
418 351 179
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 709 948
Size: 304 405 239
Fragments arranged by size:
405 304 239
BsiEI CG_ry'CG
Cuts at: 0 332 365 494 506 518 524 755 767
Size: 332 33 129 12 12 6 231 12
Cuts at: 767 948
Size: 181
Fragments arranged by size:
332 231 181 129 33 12 12 12
6
BsmBI CGTCTCn'nnnn_
Cuts at: 0 271 948
Size: 271 677
Bsp1286I G_dGCh'C
Cuts at: 0 220 263 916 948
Size: 220 43 653 32
Fragments arranged by size:
653 220 43 32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 276 948
Size: 276 672
BsrFI r'CCGG_y
Cuts at: 0 105 360 754 948
Size: 105 255 394 194
Fragments arranged by size:
394 255 194 105
BssSI C'ACGA_G
Cuts at: 0 115 948
Size: 115 833
BstAPI GCAn_nnn'nTGC
Cuts at: 0 204 948
Size: 204 744
BtgI C'CryG_G
Cuts at: 0 51 316 749 761 948
Size: 51 265 433 12 187
Fragments arranged by size:
433 265 187 51 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 881 948
Size: 881 67
BtsI GCAGTG_nn'
Cuts at: 0 238 948
Size: 238 710
EaeI y'GGCC_r
Cuts at: 0 149 362 699 752 764 948
Size: 149 213 337 53 12 184
Fragments arranged by size:
337 213 184 149 53 12
EagI C'GGCC_G
Cuts at: 0 362 752 764 948
Size: 362 390 12 184
Fragments arranged by size:
390 362 184 12
EarI CTCTTCn'nnn_
Cuts at: 0 103 948
Size: 103 845
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 563 948
Size: 449 114 385
Fragments arranged by size:
449 385 114
EcoNI CCTnn'n_nnAGG
Cuts at: 0 388 948
Size: 388 560
EcoO109I rG'GnC_Cy
Cuts at: 0 56 216 217 948
Size: 56 160 1 731
Fragments arranged by size:
731 160 56 1
FspI TGC'GCA
Cuts at: 0 76 948
Size: 76 872
FspAI rTGC'GCAy
Cuts at: 0 76 948
Size: 76 872
HaeII r_GCGC'y
Cuts at: 0 86 297 316 397 658 671 776 850
Size: 86 211 19 81 261 13 105 74
Cuts at: 850 899 948
Size: 49 49
Fragments arranged by size:
261 211 105 86 81 74 49 49
19 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 24 56 238 270 844 876 948
Size: 24 32 182 32 574 32 72
Fragments arranged by size:
574 182 72 32 32 32 24
KasI G'GCGC_C
Cuts at: 0 312 393 654 948
Size: 312 81 261 294
Fragments arranged by size:
312 294 261 81
MluI A'CGCG_T
Cuts at: 0 8 948
Size: 8 940
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 948
Size: 29 919
MspA1I CmG'CkG
Cuts at: 0 179 210 318 367 405 723 751 763
Size: 179 31 108 49 38 318 28 12
Cuts at: 763 948
Size: 185
Fragments arranged by size:
318 185 179 108 49 38 31 28
12
NaeI GCC'GGC
Cuts at: 0 362 756 948
Size: 362 394 192
Fragments arranged by size:
394 362 192
NarI GG'CG_CC
Cuts at: 0 313 394 655 948
Size: 313 81 261 293
Fragments arranged by size:
313 293 261 81
NgoMIV G'CCGG_C
Cuts at: 0 360 754 948
Size: 360 394 194
Fragments arranged by size:
394 360 194
NotI GC'GGCC_GC
Cuts at: 0 764 948
Size: 764 184
NspI r_CATG'y
Cuts at: 0 207 948
Size: 207 741
PpuMI rG'GwC_Cy
Cuts at: 0 56 948
Size: 56 892
PspOMI G'GGCC_C
Cuts at: 0 216 948
Size: 216 732
PspXI vC'TCGA_Gb
Cuts at: 0 355 385 948
Size: 355 30 563
Fragments arranged by size:
563 355 30
PstI C_TGCA'G
Cuts at: 0 140 233 948
Size: 140 93 715
Fragments arranged by size:
715 140 93
PvuII CAG'CTG
Cuts at: 0 405 723 948
Size: 405 318 225
Fragments arranged by size:
405 318 225
SacII CC_GC'GG
Cuts at: 0 319 752 764 948
Size: 319 433 12 184
Fragments arranged by size:
433 319 184 12
ScaI AGT'ACT
Cuts at: 0 68 948
Size: 68 880
SfcI C'TryA_G
Cuts at: 0 136 229 948
Size: 136 93 719
Fragments arranged by size:
719 136 93
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 148 948
Size: 148 800
SfoI GGC'GCC
Cuts at: 0 314 395 656 948
Size: 314 81 261 292
Fragments arranged by size:
314 292 261 81
SmlI C'TyrA_G
Cuts at: 0 355 385 659 948
Size: 355 30 274 289
Fragments arranged by size:
355 289 274 30
SpeI A'CTAG_T
Cuts at: 0 69 948
Size: 69 879
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 253 948
Size: 253 695
TatI w'GTAC_w
Cuts at: 0 66 948
Size: 66 882
XhoI C'TCGA_G
Cuts at: 0 355 385 948
Size: 355 30 563
Fragments arranged by size:
563 355 30
ZraI GAC'GTC
Cuts at: 0 745 948
Size: 745 203
Enzymes that do cut:
AarI AatII AflIII AlwNI ApaI AvaI BanI BanII
BbeI BcgI BglI BlpI Bme1580I BmrI BpmI BpuEI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I
BspMI BsrFI BssSI BstAPI BtgI BtgZI BtsI EaeI
EagI EarI Eco57MI EcoNI EcoO109I FspI FspAI HaeII
Hin4I KasI MluI MmeI MspA1I NaeI NarI NgoMIV
NotI NspI PpuMI PspOMI PspXI PstI PvuII SacII
ScaI SfcI SfiI SfoI SmlI SpeI TaqII TatI
XhoI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BciVI BclI BfrBI
BglII BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI
BsaXI BsiHKAI BsiWI BsmI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BstBI BstEII BstXI BstYI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EciI EcoICRI EcoRI
EcoRV FalI FseI HincII HindIII HpaI KpnI MfeI
MscI MslI NcoI NdeI NheI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PsrI PvuI RsrII SacI SalI SanDI SapI
SbfI SexAI SgfI SgrAI SmaI SnaBI SphI SrfI
SspI StuI StyI SwaI TstI Tth111I XbaI XcmI
XmaI XmnI