Restriction map for
pFN21AB8941
(Linear) (Six-base) MAPSORT of: KIBB8941.seq Check: 2406 from: 1 to: 903
KIBB8941.orf
With 182 enzymes: SgfI *
December 5, 2014 12:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 376 903
Size: 376 527
AccI GT'mk_AC
Cuts at: 0 859 903
Size: 859 44
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 903
Size: 491 412
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 646 678 903
Size: 646 32 225
Fragments arranged by size:
646 225 32
ApaI G_GGCC'C
Cuts at: 0 354 693 903
Size: 354 339 210
Fragments arranged by size:
354 339 210
AvaI C'yCGr_G
Cuts at: 0 243 339 903
Size: 243 96 564
Fragments arranged by size:
564 243 96
BanI G'GyrC_C
Cuts at: 0 219 903
Size: 219 684
BanII G_rGCy'C
Cuts at: 0 354 604 693 725 903
Size: 354 250 89 32 178
Fragments arranged by size:
354 250 178 89 32
Bme1580I G_kGCm'C
Cuts at: 0 224 354 693 903
Size: 224 130 339 210
Fragments arranged by size:
339 224 210 130
BmrI ACTGGGnnnn_n'
Cuts at: 0 602 903
Size: 602 301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 739 860 903
Size: 739 121 43
Fragments arranged by size:
739 121 43
Bpu10I CC'TnA_GC
Cuts at: 0 107 682 903
Size: 107 575 221
Fragments arranged by size:
575 221 107
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 785 815 903
Size: 785 30 88
Fragments arranged by size:
785 88 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 466 903
Size: 466 437
BseYI C'CCAG_C
Cuts at: 0 55 194 229 271 353 807 886 903
Size: 55 139 35 42 82 454 79 17
Fragments arranged by size:
454 139 82 79 55 42 35 17
BsiHKAI G_wGCw'C
Cuts at: 0 725 903
Size: 725 178
BsmBI CGTCTCn'nnnn_
Cuts at: 0 311 903
Size: 311 592
Bsp1286I G_dGCh'C
Cuts at: 0 224 354 604 693 725 903
Size: 224 130 250 89 32 178
Fragments arranged by size:
250 224 178 130 89 32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 376 903
Size: 376 527
BsrFI r'CCGG_y
Cuts at: 0 394 459 903
Size: 394 65 444
Fragments arranged by size:
444 394 65
BtgI C'CryG_G
Cuts at: 0 524 903
Size: 524 379
EcoICRI GAG'CTC
Cuts at: 0 723 903
Size: 723 180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 739 860 903
Size: 491 248 121 43
Fragments arranged by size:
491 248 121 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 481 903
Size: 481 422
EcoO109I rG'GnC_Cy
Cuts at: 0 52 350 801 903
Size: 52 298 451 102
Fragments arranged by size:
451 298 102 52
HaeII r_GCGC'y
Cuts at: 0 62 71 80 903
Size: 62 9 9 823
Fragments arranged by size:
823 62 9 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 463 495 785 817 903
Size: 463 32 290 32 86
Fragments arranged by size:
463 290 86 32 32
HincII GTy'rAC
Cuts at: 0 117 860 903
Size: 117 743 43
Fragments arranged by size:
743 117 43
MspA1I CmG'CkG
Cuts at: 0 642 838 903
Size: 642 196 65
Fragments arranged by size:
642 196 65
NspI r_CATG'y
Cuts at: 0 866 903
Size: 866 37
PasI CC'CwG_GG
Cuts at: 0 213 903
Size: 213 690
PpuMI rG'GwC_Cy
Cuts at: 0 52 801 903
Size: 52 749 102
Fragments arranged by size:
749 102 52
PspOMI G'GGCC_C
Cuts at: 0 350 689 903
Size: 350 339 214
Fragments arranged by size:
350 339 214
PspXI vC'TCGA_Gb
Cuts at: 0 339 903
Size: 339 564
PstI C_TGCA'G
Cuts at: 0 268 903
Size: 268 635
PvuII CAG'CTG
Cuts at: 0 642 838 903
Size: 642 196 65
Fragments arranged by size:
642 196 65
RsrII CG'GwC_CG
Cuts at: 0 397 903
Size: 397 506
SacI G_AGCT'C
Cuts at: 0 725 903
Size: 725 178
SalI G'TCGA_C
Cuts at: 0 858 903
Size: 858 45
SanDI GG'GwC_CC
Cuts at: 0 52 801 903
Size: 52 749 102
Fragments arranged by size:
749 102 52
ScaI AGT'ACT
Cuts at: 0 292 903
Size: 292 611
SfcI C'TryA_G
Cuts at: 0 63 264 903
Size: 63 201 639
Fragments arranged by size:
639 201 63
SmlI C'TyrA_G
Cuts at: 0 339 903
Size: 339 564
StuI AGG'CCT
Cuts at: 0 596 903
Size: 596 307
StyI C'CwwG_G
Cuts at: 0 827 903
Size: 827 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 582 900 903
Size: 582 318 3
Fragments arranged by size:
582 318 3
TatI w'GTAC_w
Cuts at: 0 290 903
Size: 290 613
XhoI C'TCGA_G
Cuts at: 0 339 903
Size: 339 564
XmnI GAAnn'nnTTC
Cuts at: 0 22 903
Size: 22 881
Enzymes that do cut:
AarI AccI AcuI AloI ApaI AvaI BanI BanII
Bme1580I BmrI BpmI Bpu10I BsaXI BseRI BseYI BsiHKAI
BsmBI Bsp1286I BspMI BsrFI BtgI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII MspA1I NspI PasI PpuMI
PspOMI PspXI PstI PvuII RsrII SacI SalI SanDI
ScaI SfcI SmlI StuI StyI TaqII TatI XhoI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI
BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PsrI PvuI SacII SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI
XcmI XmaI ZraI