Restriction map for
pFN21AB8930
(Linear) (Six-base) MAPSORT of: KIBB8930.seq Check: 1381 from: 1 to: 843
KIBB8930.orf
With 182 enzymes: SgfI *
December 5, 2014 12:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 74 182 843
Size: 74 108 661
Fragments arranged by size:
661 108 74
AfeI AGC'GCT
Cuts at: 0 427 843
Size: 427 416
AhdI GACnn_n'nnGTC
Cuts at: 0 573 843
Size: 573 270
AlwNI CAG_nnn'CTG
Cuts at: 0 483 843
Size: 483 360
ApaI G_GGCC'C
Cuts at: 0 595 843
Size: 595 248
AvaI C'yCGr_G
Cuts at: 0 631 732 843
Size: 631 101 111
Fragments arranged by size:
631 111 101
BanI G'GyrC_C
Cuts at: 0 27 718 742 843
Size: 27 691 24 101
Fragments arranged by size:
691 101 27 24
BanII G_rGCy'C
Cuts at: 0 272 595 843
Size: 272 323 248
Fragments arranged by size:
323 272 248
BbeI G_GCGC'C
Cuts at: 0 31 722 746 843
Size: 31 691 24 97
Fragments arranged by size:
691 97 31 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 824 843
Size: 824 19
BglI GCCn_nnn'nGGC
Cuts at: 0 727 843
Size: 727 116
BglII A'GATC_T
Cuts at: 0 407 843
Size: 407 436
BlpI GC'TnA_GC
Cuts at: 0 533 843
Size: 533 310
Bme1580I G_kGCm'C
Cuts at: 0 595 843
Size: 595 248
BmgBI CAC'GTC
Cuts at: 0 834 843
Size: 834 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 598 843
Size: 598 245
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 657 843
Size: 657 186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 316 571 843
Size: 283 33 255 272
Fragments arranged by size:
283 272 255 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 843
Size: 523 320
BsaBI GATnn'nnATC
Cuts at: 0 610 843
Size: 610 233
BsaHI Gr'CG_yC
Cuts at: 0 28 100 719 743 843
Size: 28 72 619 24 100
Fragments arranged by size:
619 100 72 28 24
BsaWI w'CCGG_w
Cuts at: 0 702 843
Size: 702 141
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 52 280 568 843
Size: 52 228 288 275
Fragments arranged by size:
288 275 228 52
BseYI C'CCAG_C
Cuts at: 0 483 595 843
Size: 483 112 248
Fragments arranged by size:
483 248 112
BsiEI CG_ry'CG
Cuts at: 0 462 843
Size: 462 381
BsiHKAI G_wGCw'C
Cuts at: 0 272 843
Size: 272 571
Bsp1286I G_dGCh'C
Cuts at: 0 272 595 843
Size: 272 323 248
Fragments arranged by size:
323 272 248
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 74 182 843
Size: 74 108 661
Fragments arranged by size:
661 108 74
BsrBI CCG'CTC
Cuts at: 0 42 771 843
Size: 42 729 72
Fragments arranged by size:
729 72 42
BsrFI r'CCGG_y
Cuts at: 0 655 783 843
Size: 655 128 60
Fragments arranged by size:
655 128 60
BssHII G'CGCG_C
Cuts at: 0 227 257 528 843
Size: 227 30 271 315
Fragments arranged by size:
315 271 227 30
BstXI CCAn_nnnn'nTGG
Cuts at: 0 588 843
Size: 588 255
BstYI r'GATC_y
Cuts at: 0 407 843
Size: 407 436
BtgI C'CryG_G
Cuts at: 0 185 843
Size: 185 658
EaeI y'GGCC_r
Cuts at: 0 179 623 785 843
Size: 179 444 162 58
Fragments arranged by size:
444 179 162 58
EarI CTCTTCn'nnn_
Cuts at: 0 818 843
Size: 818 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 62 567 843
Size: 62 505 276
Fragments arranged by size:
505 276 62
EcoICRI GAG'CTC
Cuts at: 0 270 843
Size: 270 573
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 316 571 843
Size: 283 33 255 272
Fragments arranged by size:
283 272 255 33
EcoO109I rG'GnC_Cy
Cuts at: 0 546 592 729 843
Size: 546 46 137 114
Fragments arranged by size:
546 137 114 46
HaeII r_GCGC'y
Cuts at: 0 31 429 622 722 740 746 843
Size: 31 398 193 100 18 6 97
Fragments arranged by size:
398 193 100 97 31 18 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 559 591 843
Size: 559 32 252
Fragments arranged by size:
559 252 32
KasI G'GCGC_C
Cuts at: 0 27 718 742 843
Size: 27 691 24 101
Fragments arranged by size:
691 101 27 24
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 843
Size: 110 733
MspA1I CmG'CkG
Cuts at: 0 57 187 252 354 483 599 843
Size: 57 130 65 102 129 116 244
Fragments arranged by size:
244 130 129 116 102 65 57
NaeI GCC'GGC
Cuts at: 0 657 785 843
Size: 657 128 58
Fragments arranged by size:
657 128 58
NarI GG'CG_CC
Cuts at: 0 28 719 743 843
Size: 28 691 24 100
Fragments arranged by size:
691 100 28 24
NgoMIV G'CCGG_C
Cuts at: 0 655 783 843
Size: 655 128 60
Fragments arranged by size:
655 128 60
NruI TCG'CGA
Cuts at: 0 464 843
Size: 464 379
PpuMI rG'GwC_Cy
Cuts at: 0 546 843
Size: 546 297
PspOMI G'GGCC_C
Cuts at: 0 591 843
Size: 591 252
PspXI vC'TCGA_Gb
Cuts at: 0 631 843
Size: 631 212
PstI C_TGCA'G
Cuts at: 0 314 752 843
Size: 314 438 91
Fragments arranged by size:
438 314 91
PvuI CG_AT'CG
Cuts at: 0 462 843
Size: 462 381
PvuII CAG'CTG
Cuts at: 0 483 843
Size: 483 360
SacI G_AGCT'C
Cuts at: 0 272 843
Size: 272 571
SacII CC_GC'GG
Cuts at: 0 188 843
Size: 188 655
SexAI A'CCwGG_T
Cuts at: 0 511 843
Size: 511 332
SfcI C'TryA_G
Cuts at: 0 310 748 843
Size: 310 438 95
Fragments arranged by size:
438 310 95
SfoI GGC'GCC
Cuts at: 0 29 720 744 843
Size: 29 691 24 99
Fragments arranged by size:
691 99 29 24
SmaI CCC'GGG
Cuts at: 0 734 843
Size: 734 109
SmlI C'TyrA_G
Cuts at: 0 538 631 843
Size: 538 93 212
Fragments arranged by size:
538 212 93
StyI C'CwwG_G
Cuts at: 0 8 843
Size: 8 835
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 603 843
Size: 603 240
XhoI C'TCGA_G
Cuts at: 0 631 843
Size: 631 212
XmaI C'CCGG_G
Cuts at: 0 732 843
Size: 732 111
Enzymes that do cut:
AarI AfeI AhdI AlwNI ApaI AvaI BanI BanII
BbeI BbsI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BstXI
BstYI BtgI EaeI EarI EciI EcoICRI Eco57MI EcoO109I
HaeII Hin4I KasI MmeI MspA1I NaeI NarI NgoMIV
NruI PpuMI PspOMI PspXI PstI PvuI PvuII SacI
SacII SexAI SfcI SfoI SmaI SmlI StyI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BmtI Bpu10I BsaI BsaAI BsaXI BsgI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MscI MslI NcoI NdeI NheI NotI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PsrI RsrII SalI SanDI SapI SbfI
ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI TstI Tth111I XbaI
XmnI ZraI