Restriction map for
pFN21AB8915
(Linear) (Six-base) MAPSORT of: KIBB8915.seq Check: 1994 from: 1 to: 771
KIBB8915.orf
With 182 enzymes: SgfI *
March 22, 2012 15:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 172 578 771
Size: 172 406 193
Fragments arranged by size:
406 193 172
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 205 237 771
Size: 205 32 534
Fragments arranged by size:
534 205 32
ApaI G_GGCC'C
Cuts at: 0 280 771
Size: 280 491
ApaLI G'TGCA_C
Cuts at: 0 149 771
Size: 149 622
BanI G'GyrC_C
Cuts at: 0 339 566 617 771
Size: 339 227 51 154
Fragments arranged by size:
339 227 154 51
BanII G_rGCy'C
Cuts at: 0 79 280 377 771
Size: 79 201 97 394
Fragments arranged by size:
394 201 97 79
BbvCI CC'TCA_GC
Cuts at: 0 329 771
Size: 329 442
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 672 706 771
Size: 672 34 65
Fragments arranged by size:
672 65 34
BlpI GC'TnA_GC
Cuts at: 0 481 771
Size: 481 290
Bme1580I G_kGCm'C
Cuts at: 0 153 280 344 569 622 771
Size: 153 127 64 225 53 149
Fragments arranged by size:
225 153 149 127 64 53
BmrI ACTGGGnnnn_n'
Cuts at: 0 597 771
Size: 597 174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 388 478 489 496 771
Size: 63 325 90 11 7 275
Fragments arranged by size:
325 275 90 63 11 7
Bpu10I CC'TnA_GC
Cuts at: 0 329 766 771
Size: 329 437 5
Fragments arranged by size:
437 329 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 337 655 771
Size: 70 267 318 116
Fragments arranged by size:
318 267 116 70
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 385 643 771
Size: 385 258 128
Fragments arranged by size:
385 258 128
BseYI C'CCAG_C
Cuts at: 0 272 343 376 416 621 771
Size: 272 71 33 40 205 150
Fragments arranged by size:
272 205 150 71 40 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 771
Size: 457 314
BsiHKAI G_wGCw'C
Cuts at: 0 7 79 153 771
Size: 7 72 74 618
Fragments arranged by size:
618 74 72 7
Bsp1286I G_dGCh'C
Cuts at: 0 7 79 153 280 344 377 569 622
Size: 7 72 74 127 64 33 192 53
Cuts at: 622 771
Size: 149
Fragments arranged by size:
192 149 127 74 72 64 53 33
7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 172 224 578 771
Size: 172 52 354 193
Fragments arranged by size:
354 193 172 52
BsrFI r'CCGG_y
Cuts at: 0 131 771
Size: 131 640
BstXI CCAn_nnnn'nTGG
Cuts at: 0 198 227 771
Size: 198 29 544
Fragments arranged by size:
544 198 29
Bsu36I CC'TnA_GG
Cuts at: 0 208 771
Size: 208 563
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 142 720 771
Size: 142 578 51
Fragments arranged by size:
578 142 51
BtsI GCAGTG_nn'
Cuts at: 0 390 399 445 771
Size: 390 9 46 326
Fragments arranged by size:
390 326 46 9
DraIII CAC_nnn'GTG
Cuts at: 0 591 771
Size: 591 180
EaeI y'GGCC_r
Cuts at: 0 188 396 771
Size: 188 208 375
Fragments arranged by size:
375 208 188
EarI CTCTTCn'nnn_
Cuts at: 0 289 526 709 771
Size: 289 237 183 62
Fragments arranged by size:
289 237 183 62
EcoICRI GAG'CTC
Cuts at: 0 77 771
Size: 77 694
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 388 478 489 496 771
Size: 63 325 90 11 7 275
Fragments arranged by size:
325 275 90 63 11 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 207 574 771
Size: 207 367 197
Fragments arranged by size:
367 207 197
EcoO109I rG'GnC_Cy
Cuts at: 0 20 276 277 333 699 771
Size: 20 256 1 56 366 72
Fragments arranged by size:
366 256 72 56 20 1
HincII GTy'rAC
Cuts at: 0 732 771
Size: 732 39
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 771
Size: 706 65
MscI TGG'CCA
Cuts at: 0 190 398 771
Size: 190 208 373
Fragments arranged by size:
373 208 190
MspA1I CmG'CkG
Cuts at: 0 113 234 252 771
Size: 113 121 18 519
Fragments arranged by size:
519 121 113 18
NspI r_CATG'y
Cuts at: 0 759 771
Size: 759 12
PfoI T'CCnGG_A
Cuts at: 0 605 771
Size: 605 166
PpuMI rG'GwC_Cy
Cuts at: 0 699 771
Size: 699 72
PshAI GACnn'nnGTC
Cuts at: 0 646 771
Size: 646 125
PspOMI G'GGCC_C
Cuts at: 0 276 771
Size: 276 495
PstI C_TGCA'G
Cuts at: 0 266 575 771
Size: 266 309 196
Fragments arranged by size:
309 266 196
PvuII CAG'CTG
Cuts at: 0 234 771
Size: 234 537
SacI G_AGCT'C
Cuts at: 0 79 771
Size: 79 692
SfcI C'TryA_G
Cuts at: 0 262 571 771
Size: 262 309 200
Fragments arranged by size:
309 262 200
SmlI C'TyrA_G
Cuts at: 0 85 352 670 771
Size: 85 267 318 101
Fragments arranged by size:
318 267 101 85
StuI AGG'CCT
Cuts at: 0 175 542 771
Size: 175 367 229
Fragments arranged by size:
367 229 175
StyI C'CwwG_G
Cuts at: 0 176 488 630 771
Size: 176 312 142 141
Fragments arranged by size:
312 176 142 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 579 771
Size: 579 192
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 601 633 771
Size: 601 32 138
Fragments arranged by size:
601 138 32
Enzymes that do cut:
AarI AloI ApaI ApaLI BanI BanII BbvCI BcgI
BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrFI BstXI Bsu36I BtgZI
BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
HincII MmeI MscI MspA1I NspI PfoI PpuMI PshAI
PspOMI PstI PvuII SacI SfcI SmlI StuI StyI
TaqII TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BciVI
BclI BfrBI BglI BglII BmgBI BmtI BplI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI ClaI CspCI
DraI DrdI EagI EciI EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HindIII HpaI KasI KpnI
MfeI MluI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TatI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI