Restriction map for
pFN21AB8906
(Linear) (Six-base) MAPSORT of: KIBB8906.seq Check: 294 from: 1 to: 717
KIBB8906.orf
With 182 enzymes: SgfI *
December 5, 2014 12:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 80 717
Size: 80 637
Acc65I G'GTAC_C
Cuts at: 0 320 717
Size: 320 397
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 717
Size: 436 281
AlwNI CAG_nnn'CTG
Cuts at: 0 288 717
Size: 288 429
AvaI C'yCGr_G
Cuts at: 0 52 334 717
Size: 52 282 383
Fragments arranged by size:
383 282 52
BanI G'GyrC_C
Cuts at: 0 320 510 595 717
Size: 320 190 85 122
Fragments arranged by size:
320 190 122 85
BanII G_rGCy'C
Cuts at: 0 235 717
Size: 235 482
BbeI G_GCGC'C
Cuts at: 0 599 717
Size: 599 118
BglII A'GATC_T
Cuts at: 0 386 717
Size: 386 331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 310 416 587 717
Size: 196 114 106 171 130
Fragments arranged by size:
196 171 130 114 106
Bpu10I CC'TnA_GC
Cuts at: 0 19 717
Size: 19 698
BsaI GGTCTCn'nnnn_
Cuts at: 0 172 708 717
Size: 172 536 9
Fragments arranged by size:
536 172 9
BsaHI Gr'CG_yC
Cuts at: 0 15 239 596 717
Size: 15 224 357 121
Fragments arranged by size:
357 224 121 15
BseYI C'CCAG_C
Cuts at: 0 104 717
Size: 104 613
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 717
Size: 460 257
BsiEI CG_ry'CG
Cuts at: 0 208 717
Size: 208 509
BsmBI CGTCTCn'nnnn_
Cuts at: 0 230 717
Size: 230 487
Bsp1286I G_dGCh'C
Cuts at: 0 235 717
Size: 235 482
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 80 369 717
Size: 80 289 348
Fragments arranged by size:
348 289 80
BsrBI CCG'CTC
Cuts at: 0 300 717
Size: 300 417
BsrFI r'CCGG_y
Cuts at: 0 513 717
Size: 513 204
BssHII G'CGCG_C
Cuts at: 0 420 717
Size: 420 297
BstAPI GCAn_nnn'nTGC
Cuts at: 0 72 717
Size: 72 645
BstYI r'GATC_y
Cuts at: 0 386 717
Size: 386 331
BtgI C'CryG_G
Cuts at: 0 575 717
Size: 575 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 388 717
Size: 388 329
EaeI y'GGCC_r
Cuts at: 0 2 144 205 717
Size: 2 142 61 512
Fragments arranged by size:
512 142 61 2
EagI C'GGCC_G
Cuts at: 0 205 717
Size: 205 512
EarI CTCTTCn'nnn_
Cuts at: 0 670 717
Size: 670 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 456 457 717
Size: 456 1 260
Fragments arranged by size:
456 260 1
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 310 416 436 587 717
Size: 196 114 106 20 151 130
Fragments arranged by size:
196 151 130 114 106 20
FspI TGC'GCA
Cuts at: 0 34 717
Size: 34 683
HaeII r_GCGC'y
Cuts at: 0 306 599 658 717
Size: 306 293 59 59
Fragments arranged by size:
306 293 59 59
KasI G'GCGC_C
Cuts at: 0 595 717
Size: 595 122
KpnI G_GTAC'C
Cuts at: 0 324 717
Size: 324 393
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 717
Size: 275 442
MscI TGG'CCA
Cuts at: 0 4 717
Size: 4 713
MspA1I CmG'CkG
Cuts at: 0 204 318 348 577 717
Size: 204 114 30 229 140
Fragments arranged by size:
229 204 140 114 30
NarI GG'CG_CC
Cuts at: 0 596 717
Size: 596 121
NruI TCG'CGA
Cuts at: 0 527 717
Size: 527 190
PfoI T'CCnGG_A
Cuts at: 0 706 717
Size: 706 11
PvuII CAG'CTG
Cuts at: 0 348 717
Size: 348 369
SacII CC_GC'GG
Cuts at: 0 578 717
Size: 578 139
SfoI GGC'GCC
Cuts at: 0 597 717
Size: 597 120
StyI C'CwwG_G
Cuts at: 0 275 717
Size: 275 442
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 631 717
Size: 631 86
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 139 171 673 705 717
Size: 139 32 502 32 12
Fragments arranged by size:
502 139 32 32 12
XbaI T'CTAG_A
Cuts at: 0 42 717
Size: 42 675
Enzymes that do cut:
AarI Acc65I AcuI AlwNI AvaI BanI BanII BbeI
BglII BpmI Bpu10I BsaI BsaHI BseYI BsgI BsiEI
BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstYI
BtgI BtgZI EaeI EagI EarI EciI Eco57MI FspI
HaeII KasI KpnI MmeI MscI MspA1I NarI NruI
PfoI PvuII SacII SfoI StyI TaqII TstI XbaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI
BplI BpuEI BsaAI BsaBI BsaWI BsaXI BseRI BsiHKAI
BsiWI BsmI BspEI BspHI BsrDI BsrGI BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI
DraIII DrdI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspAI Hin4I HincII HindIII HpaI MfeI MluI
MslI NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI Tth111I XcmI
XhoI XmaI XmnI ZraI