Restriction map for
pFN21AB8888
(Linear) (Six-base) MAPSORT of: KIBB8888.seq Check: 3706 from: 1 to: 603
KIBB8888.orf
With 182 enzymes: SgfI *
December 5, 2014 12:54 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 406 603
Size: 406 197
ApaI G_GGCC'C
Cuts at: 0 440 603
Size: 440 163
ApaLI G'TGCA_C
Cuts at: 0 124 603
Size: 124 479
AvaI C'yCGr_G
Cuts at: 0 49 96 603
Size: 49 47 507
Fragments arranged by size:
507 49 47
BanI G'GyrC_C
Cuts at: 0 24 151 603
Size: 24 127 452
Fragments arranged by size:
452 127 24
BanII G_rGCy'C
Cuts at: 0 61 103 221 317 440 603
Size: 61 42 118 96 123 163
Fragments arranged by size:
163 123 118 96 61 42
BbeI G_GCGC'C
Cuts at: 0 28 155 603
Size: 28 127 448
Fragments arranged by size:
448 127 28
BbvCI CC'TCA_GC
Cuts at: 0 547 603
Size: 547 56
BglI GCCn_nnn'nGGC
Cuts at: 0 149 209 286 603
Size: 149 60 77 317
Fragments arranged by size:
317 149 77 60
BglII A'GATC_T
Cuts at: 0 530 603
Size: 530 73
Bme1580I G_kGCm'C
Cuts at: 0 128 440 603
Size: 128 312 163
Fragments arranged by size:
312 163 128
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 345 603
Size: 130 215 258
Fragments arranged by size:
258 215 130
Bpu10I CC'TnA_GC
Cuts at: 0 547 603
Size: 547 56
BsaI GGTCTCn'nnnn_
Cuts at: 0 248 603
Size: 248 355
BsaHI Gr'CG_yC
Cuts at: 0 25 152 603
Size: 25 127 451
Fragments arranged by size:
451 127 25
BseYI C'CCAG_C
Cuts at: 0 518 603
Size: 518 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 519 603
Size: 519 84
BsiEI CG_ry'CG
Cuts at: 0 324 603
Size: 324 279
BsiHKAI G_wGCw'C
Cuts at: 0 128 603
Size: 128 475
BsmBI CGTCTCn'nnnn_
Cuts at: 0 337 603
Size: 337 266
Bsp1286I G_dGCh'C
Cuts at: 0 61 103 128 221 317 440 603
Size: 61 42 25 93 96 123 163
Fragments arranged by size:
163 123 96 93 61 42 25
BsrFI r'CCGG_y
Cuts at: 0 203 463 603
Size: 203 260 140
Fragments arranged by size:
260 203 140
BssHII G'CGCG_C
Cuts at: 0 133 244 457 459 603
Size: 133 111 213 2 144
Fragments arranged by size:
213 144 133 111 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 542 603
Size: 542 61
BstYI r'GATC_y
Cuts at: 0 530 603
Size: 530 73
BtgI C'CryG_G
Cuts at: 0 81 603
Size: 81 522
EaeI y'GGCC_r
Cuts at: 0 205 603
Size: 205 398
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 174 603
Size: 174 429
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 345 603
Size: 130 215 258
Fragments arranged by size:
258 215 130
FseI GG_CCGG'CC
Cuts at: 0 207 603
Size: 207 396
FspI TGC'GCA
Cuts at: 0 295 603
Size: 295 308
HaeII r_GCGC'y
Cuts at: 0 28 155 292 603
Size: 28 127 137 311
Fragments arranged by size:
311 137 127 28
KasI G'GCGC_C
Cuts at: 0 24 151 603
Size: 24 127 452
Fragments arranged by size:
452 127 24
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 128 603
Size: 47 81 475
Fragments arranged by size:
475 81 47
MspA1I CmG'CkG
Cuts at: 0 63 83 228 603
Size: 63 20 145 375
Fragments arranged by size:
375 145 63 20
NaeI GCC'GGC
Cuts at: 0 205 465 603
Size: 205 260 138
Fragments arranged by size:
260 205 138
NarI GG'CG_CC
Cuts at: 0 25 152 603
Size: 25 127 451
Fragments arranged by size:
451 127 25
NgoMIV G'CCGG_C
Cuts at: 0 203 463 603
Size: 203 260 140
Fragments arranged by size:
260 203 140
PspOMI G'GGCC_C
Cuts at: 0 436 603
Size: 436 167
SacII CC_GC'GG
Cuts at: 0 84 603
Size: 84 519
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 209 603
Size: 209 394
SfoI GGC'GCC
Cuts at: 0 26 153 603
Size: 26 127 450
Fragments arranged by size:
450 127 26
StuI AGG'CCT
Cuts at: 0 406 603
Size: 406 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 532 603
Size: 532 71
Enzymes that do cut:
AlfI ApaI ApaLI AvaI BanI BanII BbeI BbvCI
BglI BglII Bme1580I BpmI Bpu10I BsaI BsaHI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrFI BssHII BstAPI
BstYI BtgI EaeI EciI Eco57MI FseI FspI HaeII
KasI MmeI MspA1I NaeI NarI NgoMIV PspOMI SacII
SfiI SfoI StuI TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI AlwNI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI
BclI BfrBI BlpI BmgBI BmrI BmtI BplI BpuEI
BsaAI BsaBI BsaWI BsaXI BseRI BsiWI BsmI BspEI
BspHI BspMI BsrBI BsrDI BsrGI BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EarI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FalI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI MluI MscI MslI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StyI SwaI TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI