Restriction map for
pFN21AB8883
(Linear) (Six-base) MAPSORT of: KIBB8883.seq Check: 8230 from: 1 to: 552
KIBB8883.orf
With 182 enzymes: SgfI *
December 5, 2014 12:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 204 552
Size: 204 348
AfeI AGC'GCT
Cuts at: 0 314 552
Size: 314 238
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 118 150 552
Size: 118 32 402
Fragments arranged by size:
402 118 32
AlwNI CAG_nnn'CTG
Cuts at: 0 528 539 552
Size: 528 11 13
Fragments arranged by size:
528 13 11
ApaI G_GGCC'C
Cuts at: 0 370 412 418 552
Size: 370 42 6 134
Fragments arranged by size:
370 134 42 6
ApaLI G'TGCA_C
Cuts at: 0 190 552
Size: 190 362
AvaI C'yCGr_G
Cuts at: 0 12 273 301 411 417 552
Size: 12 261 28 110 6 135
Fragments arranged by size:
261 135 110 28 12 6
BanI G'GyrC_C
Cuts at: 0 245 277 552
Size: 245 32 275
Fragments arranged by size:
275 245 32
BanII G_rGCy'C
Cuts at: 0 13 359 370 412 418 552
Size: 13 346 11 42 6 134
Fragments arranged by size:
346 134 42 13 11 6
BbeI G_GCGC'C
Cuts at: 0 249 552
Size: 249 303
BglI GCCn_nnn'nGGC
Cuts at: 0 407 552
Size: 407 145
Bme1580I G_kGCm'C
Cuts at: 0 194 370 412 418 552
Size: 194 176 42 6 134
Fragments arranged by size:
194 176 134 42 6
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 288 552
Size: 288 264
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 283 373 502 552
Size: 145 138 90 129 50
Fragments arranged by size:
145 138 129 90 50
Bpu10I CC'TnA_GC
Cuts at: 0 7 552
Size: 7 545
BsaHI Gr'CG_yC
Cuts at: 0 246 490 552
Size: 246 244 62
Fragments arranged by size:
246 244 62
BsaWI w'CCGG_w
Cuts at: 0 80 552
Size: 80 472
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 118 148 346 376 475 505 552
Size: 118 30 198 30 99 30 47
Fragments arranged by size:
198 118 99 47 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 187 499 552
Size: 73 114 312 53
Fragments arranged by size:
312 114 73 53
BseYI C'CCAG_C
Cuts at: 0 309 552
Size: 309 243
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 232 552
Size: 100 132 320
Fragments arranged by size:
320 132 100
BsiEI CG_ry'CG
Cuts at: 0 383 552
Size: 383 169
BsiHKAI G_wGCw'C
Cuts at: 0 194 552
Size: 194 358
Bsp1286I G_dGCh'C
Cuts at: 0 13 194 359 370 412 418 552
Size: 13 181 165 11 42 6 134
Fragments arranged by size:
181 165 134 42 13 11 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 204 552
Size: 204 348
BsrBI CCG'CTC
Cuts at: 0 129 258 552
Size: 129 129 294
Fragments arranged by size:
294 129 129
BsrFI r'CCGG_y
Cuts at: 0 24 378 552
Size: 24 354 174
Fragments arranged by size:
354 174 24
BsrGI T'GTAC_A
Cuts at: 0 161 552
Size: 161 391
Bsu36I CC'TnA_GG
Cuts at: 0 200 552
Size: 200 352
BtgI C'CryG_G
Cuts at: 0 281 552
Size: 281 271
EaeI y'GGCC_r
Cuts at: 0 335 380 506 541 552
Size: 335 45 126 35 11
Fragments arranged by size:
335 126 45 35 11
EagI C'GGCC_G
Cuts at: 0 380 552
Size: 380 172
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 283 373 502 552
Size: 145 138 90 129 50
Fragments arranged by size:
145 138 129 90 50
EcoNI CCTnn'n_nnAGG
Cuts at: 0 199 484 552
Size: 199 285 68
Fragments arranged by size:
285 199 68
EcoO109I rG'GnC_Cy
Cuts at: 0 63 306 367 536 552
Size: 63 243 61 169 16
Fragments arranged by size:
243 169 63 61 16
HaeII r_GCGC'y
Cuts at: 0 249 316 552
Size: 249 67 236
Fragments arranged by size:
249 236 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 475 507 552
Size: 475 32 45
Fragments arranged by size:
475 45 32
KasI G'GCGC_C
Cuts at: 0 245 552
Size: 245 307
MscI TGG'CCA
Cuts at: 0 508 552
Size: 508 44
MspA1I CmG'CkG
Cuts at: 0 114 123 552
Size: 114 9 429
Fragments arranged by size:
429 114 9
NaeI GCC'GGC
Cuts at: 0 26 380 552
Size: 26 354 172
Fragments arranged by size:
354 172 26
NarI GG'CG_CC
Cuts at: 0 246 552
Size: 246 306
NgoMIV G'CCGG_C
Cuts at: 0 24 378 552
Size: 24 354 174
Fragments arranged by size:
354 174 24
NspI r_CATG'y
Cuts at: 0 520 552
Size: 520 32
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 118 150 552
Size: 118 32 402
Fragments arranged by size:
402 118 32
PspOMI G'GGCC_C
Cuts at: 0 366 408 414 552
Size: 366 42 6 138
Fragments arranged by size:
366 138 42 6
PvuII CAG'CTG
Cuts at: 0 114 123 552
Size: 114 9 429
Fragments arranged by size:
429 114 9
RsrII CG'GwC_CG
Cuts at: 0 373 552
Size: 373 179
SfoI GGC'GCC
Cuts at: 0 247 552
Size: 247 305
SmaI CCC'GGG
Cuts at: 0 14 413 419 552
Size: 14 399 6 133
Fragments arranged by size:
399 133 14 6
SrfI GCCC'GGGC
Cuts at: 0 14 413 552
Size: 14 399 139
Fragments arranged by size:
399 139 14
StuI AGG'CCT
Cuts at: 0 142 392 552
Size: 142 250 160
Fragments arranged by size:
250 160 142
StyI C'CwwG_G
Cuts at: 0 185 509 552
Size: 185 324 43
Fragments arranged by size:
324 185 43
TatI w'GTAC_w
Cuts at: 0 161 552
Size: 161 391
XmaI C'CCGG_G
Cuts at: 0 12 411 417 552
Size: 12 399 6 135
Fragments arranged by size:
399 135 12 6
Enzymes that do cut:
AarI AfeI AloI AlwNI ApaI ApaLI AvaI BanI
BanII BbeI BglI Bme1580I BplI BpmI Bpu10I BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrBI BsrFI BsrGI Bsu36I BtgI EaeI EagI
Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MscI MspA1I
NaeI NarI NgoMIV NspI PpiI PspOMI PvuII RsrII
SfoI SmaI SrfI StuI StyI TatI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BpuEI BsaI BsaAI
BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrDI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EarI EciI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MmeI MslI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI SspI SwaI TaqII TstI Tth111I
XbaI XcmI XhoI XmnI ZraI