Restriction map for
pFN21AB8821
(Linear) (Six-base) MAPSORT of: KIBB8821.seq Check: 6626 from: 1 to: 1152
KIBB8821.orf
With 182 enzymes: SgfI *
December 27, 2011 16:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 704 1152
Size: 704 448
AflIII A'CryG_T
Cuts at: 0 1007 1152
Size: 1007 145
AgeI A'CCGG_T
Cuts at: 0 37 1152
Size: 37 1115
AleI CACnn'nnGTG
Cuts at: 0 900 1096 1152
Size: 900 196 56
Fragments arranged by size:
900 196 56
AlwNI CAG_nnn'CTG
Cuts at: 0 239 453 801 897 1152
Size: 239 214 348 96 255
Fragments arranged by size:
348 255 239 214 96
ApaI G_GGCC'C
Cuts at: 0 766 1152
Size: 766 386
AvaI C'yCGr_G
Cuts at: 0 271 428 741 1152
Size: 271 157 313 411
Fragments arranged by size:
411 313 271 157
BanI G'GyrC_C
Cuts at: 0 432 464 537 550 745 772 936 1126
Size: 432 32 73 13 195 27 164 190
Cuts at: 1126 1152
Size: 26
Fragments arranged by size:
432 195 190 164 73 32 27 26
13
BanII G_rGCy'C
Cuts at: 0 278 302 766 1152
Size: 278 24 464 386
Fragments arranged by size:
464 386 278 24
BbeI G_GCGC'C
Cuts at: 0 436 468 554 749 776 1152
Size: 436 32 86 195 27 376
Fragments arranged by size:
436 376 195 86 32 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 867 1152
Size: 867 285
BbvCI CC'TCA_GC
Cuts at: 0 232 1152
Size: 232 920
BglI GCCn_nnn'nGGC
Cuts at: 0 595 770 1152
Size: 595 175 382
Fragments arranged by size:
595 382 175
Bme1580I G_kGCm'C
Cuts at: 0 136 540 766 831 941 1103 1129 1152
Size: 136 404 226 65 110 162 26 23
Fragments arranged by size:
404 226 162 136 110 65 26 23
BmrI ACTGGGnnnn_n'
Cuts at: 0 321 1132 1152
Size: 321 811 20
Fragments arranged by size:
811 321 20
Bpu10I CC'TnA_GC
Cuts at: 0 232 1152
Size: 232 920
BsaAI yAC'GTr
Cuts at: 0 1010 1152
Size: 1010 142
BsaHI Gr'CG_yC
Cuts at: 0 433 465 551 746 773 837 1058 1152
Size: 433 32 86 195 27 64 221 94
Fragments arranged by size:
433 221 195 94 86 64 32 27
BsaWI w'CCGG_w
Cuts at: 0 37 1152
Size: 37 1115
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 28 1152
Size: 28 1124
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3 18 85 373 1036 1152
Size: 3 15 67 288 663 116
Fragments arranged by size:
663 288 116 67 15 3
BseYI C'CCAG_C
Cuts at: 0 322 357 749 794 1152
Size: 322 35 392 45 358
Fragments arranged by size:
392 358 322 45 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 685 799 970 1152
Size: 610 75 114 171 182
Fragments arranged by size:
610 182 171 114 75
BsiEI CG_ry'CG
Cuts at: 0 1076 1152
Size: 1076 76
BsiHKAI G_wGCw'C
Cuts at: 0 272 686 1152
Size: 272 414 466
Fragments arranged by size:
466 414 272
BsmBI CGTCTCn'nnnn_
Cuts at: 0 844 1152
Size: 844 308
Bsp1286I G_dGCh'C
Cuts at: 0 136 272 278 302 540 686 766 831
Size: 136 136 6 24 238 146 80 65
Cuts at: 831 941 1103 1129 1152
Size: 110 162 26 23
Fragments arranged by size:
238 162 146 136 136 110 80 65
26 24 23 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 425 704 1152
Size: 425 279 448
Fragments arranged by size:
448 425 279
BsrBI CCG'CTC
Cuts at: 0 1034 1152
Size: 1034 118
BsrFI r'CCGG_y
Cuts at: 0 37 86 286 933 1152
Size: 37 49 200 647 219
Fragments arranged by size:
647 219 200 49 37
BssSI C'ACGA_G
Cuts at: 0 163 397 514 1152
Size: 163 234 117 638
Fragments arranged by size:
638 234 163 117
BstAPI GCAn_nnn'nTGC
Cuts at: 0 708 1152
Size: 708 444
BstXI CCAn_nnnn'nTGG
Cuts at: 0 150 902 1152
Size: 150 752 250
Fragments arranged by size:
752 250 150
BtgI C'CryG_G
Cuts at: 0 541 593 820 1136 1152
Size: 541 52 227 316 16
Fragments arranged by size:
541 316 227 52 16
BtsI GCAGTG_nn'
Cuts at: 0 390 627 1112 1152
Size: 390 237 485 40
Fragments arranged by size:
485 390 237 40
DrdI GACnn_nn'nnGTC
Cuts at: 0 235 1152
Size: 235 917
EaeI y'GGCC_r
Cuts at: 0 122 140 587 884 914 956 1073 1152
Size: 122 18 447 297 30 42 117 79
Fragments arranged by size:
447 297 122 117 79 42 30 18
EagI C'GGCC_G
Cuts at: 0 1073 1152
Size: 1073 79
EarI CTCTTCn'nnn_
Cuts at: 0 62 1152
Size: 62 1090
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1018 1152
Size: 1018 134
EcoO109I rG'GnC_Cy
Cuts at: 0 763 1152
Size: 763 389
HaeII r_GCGC'y
Cuts at: 0 323 436 454 468 490 502 554 583
Size: 323 113 18 14 22 12 52 29
Cuts at: 583 601 749 776 1152
Size: 18 148 27 376
Fragments arranged by size:
376 323 148 113 52 29 27 22
18 18 14 12
KasI G'GCGC_C
Cuts at: 0 432 464 550 745 772 1152
Size: 432 32 86 195 27 380
Fragments arranged by size:
432 380 195 86 32 27
MscI TGG'CCA
Cuts at: 0 124 142 886 916 1152
Size: 124 18 744 30 236
Fragments arranged by size:
744 236 124 30 18
MslI CAynn'nnrTG
Cuts at: 0 97 900 1096 1152
Size: 97 803 196 56
Fragments arranged by size:
803 196 97 56
MspA1I CmG'CkG
Cuts at: 0 543 595 822 834 845 920 962 999
Size: 543 52 227 12 11 75 42 37
Cuts at: 999 1078 1138 1152
Size: 79 60 14
Fragments arranged by size:
543 227 79 75 60 52 42 37
14 12 11
NaeI GCC'GGC
Cuts at: 0 88 1152
Size: 88 1064
NarI GG'CG_CC
Cuts at: 0 433 465 551 746 773 1152
Size: 433 32 86 195 27 379
Fragments arranged by size:
433 379 195 86 32 27
NgoMIV G'CCGG_C
Cuts at: 0 86 1152
Size: 86 1066
NotI GC'GGCC_GC
Cuts at: 0 1073 1152
Size: 1073 79
NspI r_CATG'y
Cuts at: 0 483 1152
Size: 483 669
PasI CC'CwG_GG
Cuts at: 0 507 533 941 1152
Size: 507 26 408 211
Fragments arranged by size:
507 408 211 26
PmlI CAC'GTG
Cuts at: 0 1010 1152
Size: 1010 142
PspOMI G'GGCC_C
Cuts at: 0 762 1152
Size: 762 390
PstI C_TGCA'G
Cuts at: 0 188 1152
Size: 188 964
PvuII CAG'CTG
Cuts at: 0 962 1152
Size: 962 190
SacII CC_GC'GG
Cuts at: 0 544 596 823 1139 1152
Size: 544 52 227 316 13
Fragments arranged by size:
544 316 227 52 13
SfcI C'TryA_G
Cuts at: 0 184 1152
Size: 184 968
SfoI GGC'GCC
Cuts at: 0 434 466 552 747 774 1152
Size: 434 32 86 195 27 378
Fragments arranged by size:
434 378 195 86 32 27
SgrAI Cr'CCGG_yG
Cuts at: 0 37 1152
Size: 37 1115
SmaI CCC'GGG
Cuts at: 0 430 743 1152
Size: 430 313 409
Fragments arranged by size:
430 409 313
SphI G_CATG'C
Cuts at: 0 483 1152
Size: 483 669
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 894 1152
Size: 894 258
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 359 391 551 583 1152
Size: 359 32 160 32 569
Fragments arranged by size:
569 359 160 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 894 1152
Size: 894 258
XmaI C'CCGG_G
Cuts at: 0 428 741 1152
Size: 428 313 411
Fragments arranged by size:
428 411 313
Enzymes that do cut:
AarI AflIII AgeI AleI AlwNI ApaI AvaI BanI
BanII BbeI BbsI BbvCI BglI Bme1580I BmrI Bpu10I
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI
BstXI BtgI BtsI DrdI EaeI EagI EarI EciI
EcoO109I HaeII KasI MscI MslI MspA1I NaeI NarI
NgoMIV NotI NspI PasI PmlI PspOMI PstI PvuII
SacII SfcI SfoI SgrAI SmaI SphI TaqII TstI
XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmtI BplI BpmI BpuEI BsaI BsaBI BsiWI
BsmI BspEI BspHI BsrDI BsrGI BssHII BstBI BstEII
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI NcoI NdeI NheI NruI NsiI PacI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SmlI SnaBI SpeI SrfI
SspI StuI StyI SwaI TatI Tth111I XbaI XhoI
XmnI ZraI