Restriction map for
pFN21AB8621
(Linear) (Six-base) MAPSORT of: KIBB8621.seq Check: 7415 from: 1 to: 2610
KIBB8621.orf
With 182 enzymes: SgfI *
March 22, 2012 15:43 ..
Acc65I G'GTAC_C
Cuts at: 0 708 2610
Size: 708 1902
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 832 2610
Size: 832 1778
AflIII A'CryG_T
Cuts at: 0 1971 2610
Size: 1971 639
AleI CACnn'nnGTG
Cuts at: 0 790 876 1054 2610
Size: 790 86 178 1556
Fragments arranged by size:
1556 790 178 86
AlwNI CAG_nnn'CTG
Cuts at: 0 1962 2610
Size: 1962 648
ApoI r'AATT_y
Cuts at: 0 3 222 726 747 1296 1314 1328 1433
Size: 3 219 504 21 549 18 14 105
Cuts at: 1433 1469 2179 2467 2610
Size: 36 710 288 143
Fragments arranged by size:
710 549 504 288 219 143 105 36
21 18 14 3
AseI AT'TA_AT
Cuts at: 0 2534 2610
Size: 2534 76
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2268 2301 2610
Size: 2268 33 309
Fragments arranged by size:
2268 309 33
BanI G'GyrC_C
Cuts at: 0 516 708 2610
Size: 516 192 1902
Fragments arranged by size:
1902 516 192
BbsI GAAGACnn'nnnn_
Cuts at: 0 660 2064 2178 2610
Size: 660 1404 114 432
Fragments arranged by size:
1404 660 432 114
BciVI GTATCCnnnnn_n'
Cuts at: 0 442 2296 2610
Size: 442 1854 314
Fragments arranged by size:
1854 442 314
BclI T'GATC_A
Cuts at: 0 1562 2610
Size: 1562 1048
BfrBI ATG'CAT
Cuts at: 0 949 2610
Size: 949 1661
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 481 2610
Size: 216 265 2129
Fragments arranged by size:
2129 265 216
Bpu10I CC'TnA_GC
Cuts at: 0 1115 2610
Size: 1115 1495
BsaI GGTCTCn'nnnn_
Cuts at: 0 787 1193 1946 2610
Size: 787 406 753 664
Fragments arranged by size:
787 753 664 406
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 614 644 2610
Size: 614 30 1966
Fragments arranged by size:
1966 614 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1726 2610
Size: 1726 884
BseYI C'CCAG_C
Cuts at: 0 1463 2610
Size: 1463 1147
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 900 2610
Size: 900 1710
BsiEI CG_ry'CG
Cuts at: 0 2394 2610
Size: 2394 216
BsmI GAATG_Cn'
Cuts at: 0 148 2577 2610
Size: 148 2429 33
Fragments arranged by size:
2429 148 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 24 2610
Size: 24 2586
BsrDI GCAATG_nn'
Cuts at: 0 32 1256 2610
Size: 32 1224 1354
Fragments arranged by size:
1354 1224 32
BsrGI T'GTAC_A
Cuts at: 0 1349 2610
Size: 1349 1261
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2053 2610
Size: 2053 557
Bsu36I CC'TnA_GG
Cuts at: 0 1032 2610
Size: 1032 1578
BtgI C'CryG_G
Cuts at: 0 1787 2394 2610
Size: 1787 607 216
Fragments arranged by size:
1787 607 216
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 663 2610
Size: 663 1947
BtsI GCAGTG_nn'
Cuts at: 0 1806 2610
Size: 1806 804
DraIII CAC_nnn'GTG
Cuts at: 0 1683 2610
Size: 1683 927
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 481 832 2610
Size: 216 265 351 1778
Fragments arranged by size:
1778 351 265 216
EcoRI G'AATT_C
Cuts at: 0 3 1469 2179 2467 2610
Size: 3 1466 710 288 143
Fragments arranged by size:
1466 710 288 143 3
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2398 2610
Size: 2398 212
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 614 646 974 1006 1173 1205 2566 2598
Size: 614 32 328 32 167 32 1361 32
Cuts at: 2598 2610
Size: 12
Fragments arranged by size:
1361 614 328 167 32 32 32 32
12
HincII GTy'rAC
Cuts at: 0 2254 2610
Size: 2254 356
HindIII A'AGCT_T
Cuts at: 0 329 464 2610
Size: 329 135 2146
Fragments arranged by size:
2146 329 135
KpnI G_GTAC'C
Cuts at: 0 712 2610
Size: 712 1898
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1302 1422 2414 2469 2574 2610
Size: 1302 120 992 55 105 36
Fragments arranged by size:
1302 992 120 105 55 36
MslI CAynn'nnrTG
Cuts at: 0 790 876 1054 1621 2610
Size: 790 86 178 567 989
Fragments arranged by size:
989 790 567 178 86
NcoI C'CATG_G
Cuts at: 0 1787 2610
Size: 1787 823
NdeI CA'TA_TG
Cuts at: 0 1128 1235 2610
Size: 1128 107 1375
Fragments arranged by size:
1375 1128 107
NsiI A_TGCA'T
Cuts at: 0 951 2610
Size: 951 1659
NspI r_CATG'y
Cuts at: 0 1975 2610
Size: 1975 635
PciI A'CATG_T
Cuts at: 0 1971 2610
Size: 1971 639
PflMI CCAn_nnn'nTGG
Cuts at: 0 1683 2610
Size: 1683 927
PfoI T'CCnGG_A
Cuts at: 0 2471 2610
Size: 2471 139
PsiI TTA'TAA
Cuts at: 0 1027 2610
Size: 1027 1583
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1925 1957 2610
Size: 1925 32 653
Fragments arranged by size:
1925 653 32
PstI C_TGCA'G
Cuts at: 0 21 1591 2610
Size: 21 1570 1019
Fragments arranged by size:
1570 1019 21
ScaI AGT'ACT
Cuts at: 0 833 2482 2610
Size: 833 1649 128
Fragments arranged by size:
1649 833 128
SexAI A'CCwGG_T
Cuts at: 0 2354 2610
Size: 2354 256
SfcI C'TryA_G
Cuts at: 0 17 62 455 545 971 1362 1587 1942
Size: 17 45 393 90 426 391 225 355
Cuts at: 1942 2610
Size: 668
Fragments arranged by size:
668 426 393 391 355 225 90 45
17
StuI AGG'CCT
Cuts at: 0 1385 2610
Size: 1385 1225
StyI C'CwwG_G
Cuts at: 0 1787 2610
Size: 1787 823
TatI w'GTAC_w
Cuts at: 0 273 500 831 1349 2480 2610
Size: 273 227 331 518 1131 130
Fragments arranged by size:
1131 518 331 273 227 130
XmnI GAAnn'nnTTC
Cuts at: 0 728 2610
Size: 728 1882
Enzymes that do cut:
Acc65I AcuI AflIII AleI AlwNI ApoI AseI BaeI
BanI BbsI BciVI BclI BfrBI BpmI Bpu10I BsaI
BsaXI BseRI BseYI BsgI BsiEI BsmI BspMI BsrDI
BsrGI BstXI Bsu36I BtgI BtgZI BtsI DraIII Eco57MI
EcoRI FalI Hin4I HincII HindIII KpnI MmeI MslI
NcoI NdeI NsiI NspI PciI PflMI PfoI PsiI
PsrI PstI ScaI SexAI SfcI StuI StyI TatI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BamHI BanII BbeI BbvCI BcgI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpuEI BsaAI BsaBI
BsaHI BsaWI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI
HaeII HpaI KasI MfeI MluI MscI MspA1I NaeI
NarI NgoMIV NheI NotI NruI PacI PasI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TstI Tth111I XbaI XcmI
XhoI XmaI ZraI