Restriction map for
pFN21AB8596
(Linear) (Six-base) MAPSORT of: KIBB8596.seq Check: 5336 from: 1 to: 2694
KIBB8596.orf
With 182 enzymes: SgfI *
December 5, 2014 12:53 ..
AccI GT'mk_AC
Cuts at: 0 2152 2694
Size: 2152 542
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1342 1427 1666 1727 1940 2083 2694
Size: 1342 85 239 61 213 143 611
Fragments arranged by size:
1342 611 239 213 143 85 61
AflII C'TTAA_G
Cuts at: 0 1036 1563 2694
Size: 1036 527 1131
Fragments arranged by size:
1131 1036 527
AflIII A'CryG_T
Cuts at: 0 2556 2694
Size: 2556 138
AlwNI CAG_nnn'CTG
Cuts at: 0 784 2694
Size: 784 1910
ApoI r'AATT_y
Cuts at: 0 217 516 605 698 2538 2659 2694
Size: 217 299 89 93 1840 121 35
Fragments arranged by size:
1840 299 217 121 93 89 35
AseI AT'TA_AT
Cuts at: 0 842 2694
Size: 842 1852
BanI G'GyrC_C
Cuts at: 0 337 2448 2694
Size: 337 2111 246
Fragments arranged by size:
2111 337 246
BanII G_rGCy'C
Cuts at: 0 2335 2694
Size: 2335 359
BbsI GAAGACnn'nnnn_
Cuts at: 0 23 361 1415 1672 2129 2327 2694
Size: 23 338 1054 257 457 198 367
Fragments arranged by size:
1054 457 367 338 257 198 23
BfrBI ATG'CAT
Cuts at: 0 428 2694
Size: 428 2266
BglII A'GATC_T
Cuts at: 0 1124 1602 2694
Size: 1124 478 1092
Fragments arranged by size:
1124 1092 478
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 499 1501 1612 2694
Size: 410 89 1002 111 1082
Fragments arranged by size:
1082 1002 410 111 89
Bpu10I CC'TnA_GC
Cuts at: 0 809 882 2694
Size: 809 73 1812
Fragments arranged by size:
1812 809 73
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1579 2694
Size: 1579 1115
BsaWI w'CCGG_w
Cuts at: 0 57 802 2694
Size: 57 745 1892
Fragments arranged by size:
1892 745 57
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1510 2694
Size: 1510 1184
BseYI C'CCAG_C
Cuts at: 0 349 2694
Size: 349 2345
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2089 2694
Size: 2089 605
BsiHKAI G_wGCw'C
Cuts at: 0 2335 2694
Size: 2335 359
BsmI GAATG_Cn'
Cuts at: 0 669 1725 2424 2694
Size: 669 1056 699 270
Fragments arranged by size:
1056 699 669 270
Bsp1286I G_dGCh'C
Cuts at: 0 2335 2694
Size: 2335 359
BspEI T'CCGG_A
Cuts at: 0 57 2694
Size: 57 2637
BsrDI GCAATG_nn'
Cuts at: 0 1089 1460 2471 2694
Size: 1089 371 1011 223
Fragments arranged by size:
1089 1011 371 223
BsrFI r'CCGG_y
Cuts at: 0 1768 2694
Size: 1768 926
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2430 2694
Size: 2430 264
BstYI r'GATC_y
Cuts at: 0 210 781 1124 1602 1668 2694
Size: 210 571 343 478 66 1026
Fragments arranged by size:
1026 571 478 343 210 66
Bsu36I CC'TnA_GG
Cuts at: 0 1241 2694
Size: 1241 1453
BtgI C'CryG_G
Cuts at: 0 162 2694
Size: 162 2532
DrdI GACnn_nn'nnGTC
Cuts at: 0 1565 2694
Size: 1565 1129
EarI CTCTTCn'nnn_
Cuts at: 0 440 1072 2694
Size: 440 632 1622
Fragments arranged by size:
1622 632 440
EcoICRI GAG'CTC
Cuts at: 0 2333 2694
Size: 2333 361
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 410 499 1342 1427 1501 1612 1666 1727
Size: 410 89 843 85 74 111 54 61
Cuts at: 1727 1940 2083 2694
Size: 213 143 611
Fragments arranged by size:
843 611 410 213 143 111 89 85
74 61 54
EcoO109I rG'GnC_Cy
Cuts at: 0 815 2694
Size: 815 1879
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 232 264 489 521 633 665 888 920
Size: 232 32 225 32 112 32 223 32
Cuts at: 920 1051 1083 2284 2316 2694
Size: 131 32 1201 32 378
Fragments arranged by size:
1201 378 232 225 223 131 112 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 251 2525 2694
Size: 251 2274 169
Fragments arranged by size:
2274 251 169
HindIII A'AGCT_T
Cuts at: 0 931 2135 2694
Size: 931 1204 559
Fragments arranged by size:
1204 931 559
HpaI GTT'AAC
Cuts at: 0 2525 2694
Size: 2525 169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1307 1756 2694
Size: 1307 449 938
Fragments arranged by size:
1307 938 449
MslI CAynn'nnrTG
Cuts at: 0 658 2464 2694
Size: 658 1806 230
Fragments arranged by size:
1806 658 230
MspA1I CmG'CkG
Cuts at: 0 82 379 2694
Size: 82 297 2315
Fragments arranged by size:
2315 297 82
NaeI GCC'GGC
Cuts at: 0 1770 2694
Size: 1770 924
NcoI C'CATG_G
Cuts at: 0 162 2694
Size: 162 2532
NdeI CA'TA_TG
Cuts at: 0 32 1661 2694
Size: 32 1629 1033
Fragments arranged by size:
1629 1033 32
NgoMIV G'CCGG_C
Cuts at: 0 1768 2694
Size: 1768 926
NsiI A_TGCA'T
Cuts at: 0 430 2694
Size: 430 2264
PflMI CCAn_nnn'nTGG
Cuts at: 0 1921 2694
Size: 1921 773
PfoI T'CCnGG_A
Cuts at: 0 536 2694
Size: 536 2158
PpuMI rG'GwC_Cy
Cuts at: 0 815 2694
Size: 815 1879
PsiI TTA'TAA
Cuts at: 0 193 2694
Size: 193 2501
PstI C_TGCA'G
Cuts at: 0 468 2532 2694
Size: 468 2064 162
Fragments arranged by size:
2064 468 162
PvuII CAG'CTG
Cuts at: 0 82 379 2694
Size: 82 297 2315
Fragments arranged by size:
2315 297 82
SacI G_AGCT'C
Cuts at: 0 2335 2694
Size: 2335 359
SanDI GG'GwC_CC
Cuts at: 0 815 2694
Size: 815 1879
SapI GCTCTTCn'nnn_
Cuts at: 0 1072 2694
Size: 1072 1622
ScaI AGT'ACT
Cuts at: 0 326 462 798 2694
Size: 326 136 336 1896
Fragments arranged by size:
1896 336 326 136
SfcI C'TryA_G
Cuts at: 0 464 1985 2127 2153 2528 2694
Size: 464 1521 142 26 375 166
Fragments arranged by size:
1521 464 375 166 142 26
SmlI C'TyrA_G
Cuts at: 0 1036 1563 1594 2694
Size: 1036 527 31 1100
Fragments arranged by size:
1100 1036 527 31
SspI AAT'ATT
Cuts at: 0 840 1750 2673 2694
Size: 840 910 923 21
Fragments arranged by size:
923 910 840 21
StyI C'CwwG_G
Cuts at: 0 162 2694
Size: 162 2532
TatI w'GTAC_w
Cuts at: 0 324 460 796 2694
Size: 324 136 336 1898
Fragments arranged by size:
1898 336 324 136
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 7 39 518 550 2694
Size: 7 32 479 32 2144
Fragments arranged by size:
2144 479 32 32 7
XmnI GAAnn'nnTTC
Cuts at: 0 497 1195 1262 2673 2694
Size: 497 698 67 1411 21
Fragments arranged by size:
1411 698 497 67 21
Enzymes that do cut:
AccI AcuI AflII AflIII AlwNI ApoI AseI BanI
BanII BbsI BfrBI BglII BpmI Bpu10I BpuEI BsaWI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BsrDI
BsrFI BstAPI BstYI Bsu36I BtgI DrdI EarI EcoICRI
Eco57MI EcoO109I Hin4I HincII HindIII HpaI MmeI MslI
MspA1I NaeI NcoI NdeI NgoMIV NsiI PflMI PfoI
PpuMI PsiI PstI PvuII SacI SanDI SapI ScaI
SfcI SmlI SspI StyI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BglI
BlpI Bme1580I BmgBI BmrI BmtI BplI BsaI BsaAI
BsaBI BsaHI BsaXI BsiEI BsiWI BsmBI BspHI BspMI
BsrBI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
BtgZI BtsI ClaI CspCI DraI DraIII EaeI EagI
EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII KasI KpnI MfeI MluI MscI NarI NheI
NotI NruI NspI PacI PasI PciI PmeI PmlI
PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI TaqII Tth111I
XbaI XcmI XhoI XmaI ZraI