Restriction map for
pFN21AB8559
(Linear) (Six-base) MAPSORT of: KIBB8559.seq Check: 3143 from: 1 to: 3018
KIBB8559.orf
With 182 enzymes: SgfI *
March 22, 2012 15:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 448 2053 3018
Size: 448 1605 965
Fragments arranged by size:
1605 965 448
Acc65I G'GTAC_C
Cuts at: 0 2227 3018
Size: 2227 791
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1097 1276 1703 2929 3018
Size: 1097 179 427 1226 89
Fragments arranged by size:
1226 1097 427 179 89
AflIII A'CryG_T
Cuts at: 0 1425 2207 2535 2648 3018
Size: 1425 782 328 113 370
Fragments arranged by size:
1425 782 370 328 113
AleI CACnn'nnGTG
Cuts at: 0 2651 3018
Size: 2651 367
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1823 3018
Size: 1823 1195
ApaLI G'TGCA_C
Cuts at: 0 324 3018
Size: 324 2694
AvaI C'yCGr_G
Cuts at: 0 47 133 3018
Size: 47 86 2885
Fragments arranged by size:
2885 86 47
BanI G'GyrC_C
Cuts at: 0 109 460 985 2065 2227 3018
Size: 109 351 525 1080 162 791
Fragments arranged by size:
1080 791 525 351 162 109
BanII G_rGCy'C
Cuts at: 0 133 3018
Size: 133 2885
BbsI GAAGACnn'nnnn_
Cuts at: 0 1645 3018
Size: 1645 1373
BciVI GTATCCnnnnn_n'
Cuts at: 0 902 2091 3018
Size: 902 1189 927
Fragments arranged by size:
1189 927 902
BfrBI ATG'CAT
Cuts at: 0 1228 3018
Size: 1228 1790
BglI GCCn_nnn'nGGC
Cuts at: 0 107 3018
Size: 107 2911
BglII A'GATC_T
Cuts at: 0 762 3018
Size: 762 2256
Bme1580I G_kGCm'C
Cuts at: 0 112 328 2890 3018
Size: 112 216 2562 128
Fragments arranged by size:
2562 216 128 112
BmrI ACTGGGnnnn_n'
Cuts at: 0 1661 1820 3018
Size: 1661 159 1198
Fragments arranged by size:
1661 1198 159
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2501 3018
Size: 2501 517
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 570 1657 1775 3018
Size: 570 1087 118 1243
Fragments arranged by size:
1243 1087 570 118
Bpu10I CC'TnA_GC
Cuts at: 0 968 2296 3018
Size: 968 1328 722
Fragments arranged by size:
1328 968 722
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2763 3018
Size: 2763 255
BsaI GGTCTCn'nnnn_
Cuts at: 0 2951 3018
Size: 2951 67
BsaAI yAC'GTr
Cuts at: 0 1932 2536 3018
Size: 1932 604 482
Fragments arranged by size:
1932 604 482
BsaBI GATnn'nnATC
Cuts at: 0 350 869 2348 3018
Size: 350 519 1479 670
Fragments arranged by size:
1479 670 519 350
BsaWI w'CCGG_w
Cuts at: 0 116 3018
Size: 116 2902
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 169 199 1748 1778 2135 2165 3018
Size: 169 30 1549 30 357 30 853
Fragments arranged by size:
1549 853 357 169 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 151 491 3018
Size: 151 340 2527
Fragments arranged by size:
2527 340 151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 995 1793 2081 2273 2713 3018
Size: 320 675 798 288 192 440 305
Fragments arranged by size:
798 675 440 320 305 288 192
BsiHKAI G_wGCw'C
Cuts at: 0 328 2467 3018
Size: 328 2139 551
Fragments arranged by size:
2139 551 328
BsmI GAATG_Cn'
Cuts at: 0 284 1575 2300 3018
Size: 284 1291 725 718
Fragments arranged by size:
1291 725 718 284
Bsp1286I G_dGCh'C
Cuts at: 0 112 133 328 2467 2890 3018
Size: 112 21 195 2139 423 128
Fragments arranged by size:
2139 423 195 128 112 21
BspEI T'CCGG_A
Cuts at: 0 116 3018
Size: 116 2902
BspHI T'CATG_A
Cuts at: 0 873 1701 3018
Size: 873 828 1317
Fragments arranged by size:
1317 873 828
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 268 448 2053 3018
Size: 268 180 1605 965
Fragments arranged by size:
1605 965 268 180
BsrDI GCAATG_nn'
Cuts at: 0 1230 1387 1838 3018
Size: 1230 157 451 1180
Fragments arranged by size:
1230 1180 451 157
BsrFI r'CCGG_y
Cuts at: 0 226 3018
Size: 226 2792
BsrGI T'GTAC_A
Cuts at: 0 307 780 1014 3018
Size: 307 473 234 2004
Fragments arranged by size:
2004 473 307 234
BssHII G'CGCG_C
Cuts at: 0 218 3018
Size: 218 2800
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2263 2847 3018
Size: 2263 584 171
Fragments arranged by size:
2263 584 171
BstBI TT'CG_AA
Cuts at: 0 2304 3018
Size: 2304 714
BstEII G'GTnAC_C
Cuts at: 0 745 1285 3018
Size: 745 540 1733
Fragments arranged by size:
1733 745 540
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1282 1865 3018
Size: 1282 583 1153
Fragments arranged by size:
1282 1153 583
BstYI r'GATC_y
Cuts at: 0 762 3018
Size: 762 2256
BtgI C'CryG_G
Cuts at: 0 1864 3018
Size: 1864 1154
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 349 1096 3018
Size: 349 747 1922
Fragments arranged by size:
1922 747 349
BtsI GCAGTG_nn'
Cuts at: 0 496 687 2701 3018
Size: 496 191 2014 317
Fragments arranged by size:
2014 496 317 191
DraI TTT'AAA
Cuts at: 0 1142 3018
Size: 1142 1876
DraIII CAC_nnn'GTG
Cuts at: 0 332 3018
Size: 332 2686
EaeI y'GGCC_r
Cuts at: 0 1107 1389 2643 3018
Size: 1107 282 1254 375
Fragments arranged by size:
1254 1107 375 282
EarI CTCTTCn'nnn_
Cuts at: 0 1703 2242 2444 2470 2712 3018
Size: 1703 539 202 26 242 306
Fragments arranged by size:
1703 539 306 242 202 26
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 570 1097 1276 1657 1703 1775 2929 3018
Size: 570 527 179 381 46 72 1154 89
Fragments arranged by size:
1154 570 527 381 179 89 72 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 698 3018
Size: 698 2320
EcoRV GAT'ATC
Cuts at: 0 1731 1980 2775 3018
Size: 1731 249 795 243
Fragments arranged by size:
1731 795 249 243
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1102 1366 1642 2741 3018
Size: 1102 264 276 1099 277
Fragments arranged by size:
1102 1099 277 276 264
HaeII r_GCGC'y
Cuts at: 0 47 1532 1587 3018
Size: 47 1485 55 1431
Fragments arranged by size:
1485 1431 55 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 619 651 903 935 1161 1193 2761 2793
Size: 619 32 252 32 226 32 1568 32
Cuts at: 2793 2958 2990 3018
Size: 165 32 28
Fragments arranged by size:
1568 619 252 226 165 32 32 32
32 32 28
HincII GTy'rAC
Cuts at: 0 1431 2947 3018
Size: 1431 1516 71
Fragments arranged by size:
1516 1431 71
HindIII A'AGCT_T
Cuts at: 0 2071 3018
Size: 2071 947
KpnI G_GTAC'C
Cuts at: 0 2231 3018
Size: 2231 787
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1114 3018
Size: 1114 1904
MscI TGG'CCA
Cuts at: 0 1391 2645 3018
Size: 1391 1254 373
Fragments arranged by size:
1391 1254 373
MslI CAynn'nnrTG
Cuts at: 0 800 1399 2651 3018
Size: 800 599 1252 367
Fragments arranged by size:
1252 800 599 367
MspA1I CmG'CkG
Cuts at: 0 169 182 2623 3018
Size: 169 13 2441 395
Fragments arranged by size:
2441 395 169 13
NcoI C'CATG_G
Cuts at: 0 1864 3018
Size: 1864 1154
NdeI CA'TA_TG
Cuts at: 0 579 3018
Size: 579 2439
NsiI A_TGCA'T
Cuts at: 0 1230 3018
Size: 1230 1788
NspI r_CATG'y
Cuts at: 0 636 1429 2652 3018
Size: 636 793 1223 366
Fragments arranged by size:
1223 793 636 366
PasI CC'CwG_GG
Cuts at: 0 2232 3018
Size: 2232 786
PciI A'CATG_T
Cuts at: 0 1425 2648 3018
Size: 1425 1223 370
Fragments arranged by size:
1425 1223 370
PflMI CCAn_nnn'nTGG
Cuts at: 0 280 3018
Size: 280 2738
PfoI T'CCnGG_A
Cuts at: 0 10 209 542 653 1673 2575 3018
Size: 10 199 333 111 1020 902 443
Fragments arranged by size:
1020 902 443 333 199 111 10
PmlI CAC'GTG
Cuts at: 0 2536 3018
Size: 2536 482
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1937 1969 3018
Size: 1937 32 1049
Fragments arranged by size:
1937 1049 32
PstI C_TGCA'G
Cuts at: 0 1209 3018
Size: 1209 1809
PvuII CAG'CTG
Cuts at: 0 169 2623 3018
Size: 169 2454 395
Fragments arranged by size:
2454 395 169
ScaI AGT'ACT
Cuts at: 0 1544 3018
Size: 1544 1474
SexAI A'CCwGG_T
Cuts at: 0 749 3018
Size: 749 2269
SfcI C'TryA_G
Cuts at: 0 678 1205 3018
Size: 678 527 1813
Fragments arranged by size:
1813 678 527
SmlI C'TyrA_G
Cuts at: 0 2778 3018
Size: 2778 240
SspI AAT'ATT
Cuts at: 0 2790 3018
Size: 2790 228
StyI C'CwwG_G
Cuts at: 0 662 1193 1864 3018
Size: 662 531 671 1154
Fragments arranged by size:
1154 671 662 531
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 994 3018
Size: 994 2024
TatI w'GTAC_w
Cuts at: 0 307 780 885 1014 1113 1542 3018
Size: 307 473 105 129 99 429 1476
Fragments arranged by size:
1476 473 429 307 129 105 99
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 58 90 475 507 714 746 3018
Size: 58 32 385 32 207 32 2272
Fragments arranged by size:
2272 385 207 58 32 32 32
XbaI T'CTAG_A
Cuts at: 0 2898 3018
Size: 2898 120
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1513 3018
Size: 1513 1505
XmnI GAAnn'nnTTC
Cuts at: 0 2567 3018
Size: 2567 451
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AlfI ApaLI AvaI
BanI BanII BbsI BciVI BfrBI BglI BglII Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrDI BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII
EaeI EarI Eco57MI EcoNI EcoRV FalI HaeII Hin4I
HincII HindIII KpnI MmeI MscI MslI MspA1I NcoI
NdeI NsiI NspI PasI PciI PflMI PfoI PmlI
PsrI PstI PvuII ScaI SexAI SfcI SmlI SspI
StyI TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AloI
AlwNI ApaI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BclI BlpI BmgBI BmtI
BsaHI BseYI BsiEI BsiWI BsmBI BsrBI BssSI BstZ17I
Bsu36I ClaI CspCI DrdI EagI EciI EcoICRI EcoO109I
EcoRI FseI FspI FspAI HpaI KasI MfeI MluI
NaeI NarI NgoMIV NheI NotI NruI PacI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI Tth111I XhoI XmaI ZraI