Restriction map for
pFN21AB8543
(Linear) (Six-base) MAPSORT of: KIBB8543.seq Check: 1252 from: 1 to: 2940
KIBB8543.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1957 2940
Size: 1957 983
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 1253 1366 1495 2940
Size: 929 324 113 129 1445
Fragments arranged by size:
1445 929 324 129 113
AflIII A'CryG_T
Cuts at: 0 2324 2640 2940
Size: 2324 316 300
Fragments arranged by size:
2324 316 300
AhdI GACnn_n'nnGTC
Cuts at: 0 355 2940
Size: 355 2585
AleI CACnn'nnGTG
Cuts at: 0 1588 1875 2940
Size: 1588 287 1065
Fragments arranged by size:
1588 1065 287
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2406 2940
Size: 2406 534
AlwNI CAG_nnn'CTG
Cuts at: 0 222 508 1496 1734 2046 2898 2940
Size: 222 286 988 238 312 852 42
Fragments arranged by size:
988 852 312 286 238 222 42
ApaI G_GGCC'C
Cuts at: 0 82 451 1411 1554 1728 1821 2234 2940
Size: 82 369 960 143 174 93 413 706
Fragments arranged by size:
960 706 413 369 174 143 93 82
ApoI r'AATT_y
Cuts at: 0 875 2665 2940
Size: 875 1790 275
Fragments arranged by size:
1790 875 275
AvaI C'yCGr_G
Cuts at: 0 73 205 1547 1771 2940
Size: 73 132 1342 224 1169
Fragments arranged by size:
1342 1169 224 132 73
BanI G'GyrC_C
Cuts at: 0 187 325 1369 1479 1776 2239 2940
Size: 187 138 1044 110 297 463 701
Fragments arranged by size:
1044 701 463 297 187 138 110
BanII G_rGCy'C
Cuts at: 0 32 82 139 262 347 451 479 794
Size: 32 50 57 123 85 104 28 315
Cuts at: 794 872 1252 1411 1466 1554 1728 1772 1821
Size: 78 380 159 55 88 174 44 49
Cuts at: 1821 2088 2234 2318 2940
Size: 267 146 84 622
Fragments arranged by size:
622 380 315 267 174 159 146 123
104 88 85 84 78 57 55 50
49 44 32 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 917 971 1432 2940
Size: 917 54 461 1508
Fragments arranged by size:
1508 917 461 54
BbvCI CC'TCA_GC
Cuts at: 0 2027 2296 2940
Size: 2027 269 644
Fragments arranged by size:
2027 644 269
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1762 1796 2940
Size: 1762 34 1144
Fragments arranged by size:
1762 1144 34
BfrBI ATG'CAT
Cuts at: 0 2393 2940
Size: 2393 547
BglI GCCn_nnn'nGGC
Cuts at: 0 829 1131 1201 1415 2940
Size: 829 302 70 214 1525
Fragments arranged by size:
1525 829 302 214 70
BglII A'GATC_T
Cuts at: 0 2553 2940
Size: 2553 387
BlpI GC'TnA_GC
Cuts at: 0 1804 2823 2940
Size: 1804 1019 117
Fragments arranged by size:
1804 1019 117
Bme1580I G_kGCm'C
Cuts at: 0 82 190 318 451 1374 1411 1484 1554
Size: 82 108 128 133 923 37 73 70
Cuts at: 1554 1728 1821 2234 2940
Size: 174 93 413 706
Fragments arranged by size:
923 706 413 174 133 128 108 93
82 73 70 37
BmrI ACTGGGnnnn_n'
Cuts at: 0 265 388 772 2940
Size: 265 123 384 2168
Fragments arranged by size:
2168 384 265 123
BmtI G_CTAG'C
Cuts at: 0 1870 2940
Size: 1870 1070
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 406 811 2940
Size: 406 405 2129
Fragments arranged by size:
2129 406 405
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 190 436 565 623 1160 1289 1308
Size: 46 144 246 129 58 537 129 19
Cuts at: 1308 1333 1342 1473 2068 2173 2194 2474 2818
Size: 25 9 131 595 105 21 280 344
Cuts at: 2818 2940
Size: 122
Fragments arranged by size:
595 537 344 280 246 144 131 129
129 122 105 58 46 25 21 19
9
Bpu10I CC'TnA_GC
Cuts at: 0 1246 2027 2183 2211 2296 2940
Size: 1246 781 156 28 85 644
Fragments arranged by size:
1246 781 644 156 85 28
BsaI GGTCTCn'nnnn_
Cuts at: 0 379 2149 2940
Size: 379 1770 791
Fragments arranged by size:
1770 791 379
BsaWI w'CCGG_w
Cuts at: 0 2903 2940
Size: 2903 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2540 2570 2614 2644 2940
Size: 2540 30 44 30 296
Fragments arranged by size:
2540 296 44 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1292 1651 1654 1996 2359 2471 2940
Size: 1292 359 3 342 363 112 469
Fragments arranged by size:
1292 469 363 359 342 112 3
BseYI C'CCAG_C
Cuts at: 0 99 333 444 743 783 1620 2633 2720
Size: 99 234 111 299 40 837 1013 87
Cuts at: 2720 2940
Size: 220
Fragments arranged by size:
1013 837 299 234 220 111 99 87
40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 745 1520 1768 1901 1923 2301 2491 2940
Size: 745 775 248 133 22 378 190 449
Fragments arranged by size:
775 745 449 378 248 190 133 22
BsiEI CG_ry'CG
Cuts at: 0 41 427 2940
Size: 41 386 2513
Fragments arranged by size:
2513 386 41
BsiHKAI G_wGCw'C
Cuts at: 0 479 872 1772 2180 2318 2940
Size: 479 393 900 408 138 622
Fragments arranged by size:
900 622 479 408 393 138
BsmBI CGTCTCn'nnnn_
Cuts at: 0 412 2199 2940
Size: 412 1787 741
Fragments arranged by size:
1787 741 412
Bsp1286I G_dGCh'C
Cuts at: 0 32 82 139 190 262 318 347 451
Size: 32 50 57 51 72 56 29 104
Cuts at: 451 479 794 872 1252 1374 1411 1466 1484
Size: 28 315 78 380 122 37 55 18
Cuts at: 1484 1554 1728 1772 1821 2088 2180 2234 2318
Size: 70 174 44 49 267 92 54 84
Cuts at: 2318 2940
Size: 622
Fragments arranged by size:
622 380 315 267 174 122 104 92
84 78 72 70 57 56 55 54
51 50 49 44 37 32 29 28
18
BspHI T'CATG_A
Cuts at: 0 1764 2790 2940
Size: 1764 1026 150
Fragments arranged by size:
1764 1026 150
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 717 1742 1957 2940
Size: 717 1025 215 983
Fragments arranged by size:
1025 983 717 215
BsrDI GCAATG_nn'
Cuts at: 0 928 2940
Size: 928 2012
BsrFI r'CCGG_y
Cuts at: 0 87 147 426 1025 1562 2008 2242 2940
Size: 87 60 279 599 537 446 234 698
Fragments arranged by size:
698 599 537 446 279 234 87 60
BsrGI T'GTAC_A
Cuts at: 0 1226 2940
Size: 1226 1714
BssHII G'CGCG_C
Cuts at: 0 233 2940
Size: 233 2707
BstAPI GCAn_nnn'nTGC
Cuts at: 0 441 2898 2940
Size: 441 2457 42
Fragments arranged by size:
2457 441 42
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2080 2940
Size: 2080 860
BstYI r'GATC_y
Cuts at: 0 1097 1899 2093 2147 2553 2940
Size: 1097 802 194 54 406 387
Fragments arranged by size:
1097 802 406 387 194 54
Bsu36I CC'TnA_GG
Cuts at: 0 118 2940
Size: 118 2822
BtgI C'CryG_G
Cuts at: 0 82 406 480 539 636 1258 2226 2840
Size: 82 324 74 59 97 622 968 614
Cuts at: 2840 2940
Size: 100
Fragments arranged by size:
968 622 614 324 100 97 82 74
59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 885 2940
Size: 885 2055
BtsI GCAGTG_nn'
Cuts at: 0 65 316 1398 1823 2940
Size: 65 251 1082 425 1117
Fragments arranged by size:
1117 1082 425 251 65
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1419 1454 2940
Size: 1419 35 1486
Fragments arranged by size:
1486 1419 35
DraI TTT'AAA
Cuts at: 0 1149 2940
Size: 1149 1791
DraIII CAC_nnn'GTG
Cuts at: 0 2279 2940
Size: 2279 661
EaeI y'GGCC_r
Cuts at: 0 85 424 1202 1709 2258 2940
Size: 85 339 778 507 549 682
Fragments arranged by size:
778 682 549 507 339 85
EagI C'GGCC_G
Cuts at: 0 424 2940
Size: 424 2516
EarI CTCTTCn'nnn_
Cuts at: 0 188 655 2940
Size: 188 467 2285
Fragments arranged by size:
2285 467 188
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 109 2940
Size: 109 2831
EcoICRI GAG'CTC
Cuts at: 0 477 870 1770 2316 2940
Size: 477 393 900 546 624
Fragments arranged by size:
900 624 546 477 393
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 190 436 565 623 929 1160 1253
Size: 46 144 246 129 58 306 231 93
Cuts at: 1253 1289 1308 1333 1342 1366 1473 1495 2068
Size: 36 19 25 9 24 107 22 573
Cuts at: 2068 2173 2194 2474 2818 2940
Size: 105 21 280 344 122
Fragments arranged by size:
573 344 306 280 246 231 144 129
122 107 105 93 58 46 36 25
24 22 21 19 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 652 1669 1843 1941 2026 2940
Size: 652 1017 174 98 85 914
Fragments arranged by size:
1017 914 652 174 98 85
EcoO109I rG'GnC_Cy
Cuts at: 0 21 78 202 266 337 729 747 766
Size: 21 57 124 64 71 392 18 19
Cuts at: 766 1407 1408 1674 1731 1818 2231 2940
Size: 641 1 266 57 87 413 709
Fragments arranged by size:
709 641 413 392 266 124 87 71
64 57 57 21 19 18 1
EcoRI G'AATT_C
Cuts at: 0 875 2940
Size: 875 2065
HaeII r_GCGC'y
Cuts at: 0 7 154 307 814 2940
Size: 7 147 153 507 2126
Fragments arranged by size:
2126 507 153 147 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 341 373 2540 2572 2614 2646 2728 2760
Size: 341 32 2167 32 42 32 82 32
Cuts at: 2760 2940
Size: 180
Fragments arranged by size:
2167 341 180 82 42 32 32 32
32
MfeI C'AATT_G
Cuts at: 0 1082 2940
Size: 1082 1858
MluI A'CGCG_T
Cuts at: 0 2640 2940
Size: 2640 300
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 2061 2940
Size: 61 2000 879
Fragments arranged by size:
2000 879 61
MscI TGG'CCA
Cuts at: 0 1711 2260 2940
Size: 1711 549 680
Fragments arranged by size:
1711 680 549
MslI CAynn'nnrTG
Cuts at: 0 1588 1705 1875 2323 2329 2940
Size: 1588 117 170 448 6 611
Fragments arranged by size:
1588 611 448 170 117 6
MspA1I CmG'CkG
Cuts at: 0 366 408 482 783 1588 1688 1800 2724
Size: 366 42 74 301 805 100 112 924
Cuts at: 2724 2770 2940
Size: 46 170
Fragments arranged by size:
924 805 366 301 170 112 100 74
46 42
NaeI GCC'GGC
Cuts at: 0 89 149 428 1564 2010 2940
Size: 89 60 279 1136 446 930
Fragments arranged by size:
1136 930 446 279 89 60
NcoI C'CATG_G
Cuts at: 0 1258 2226 2840 2940
Size: 1258 968 614 100
Fragments arranged by size:
1258 968 614 100
NgoMIV G'CCGG_C
Cuts at: 0 87 147 426 1562 2008 2940
Size: 87 60 279 1136 446 932
Fragments arranged by size:
1136 932 446 279 87 60
NheI G'CTAG_C
Cuts at: 0 1866 2940
Size: 1866 1074
NsiI A_TGCA'T
Cuts at: 0 2395 2940
Size: 2395 545
NspI r_CATG'y
Cuts at: 0 2328 2940
Size: 2328 612
PasI CC'CwG_GG
Cuts at: 0 451 1043 1839 2940
Size: 451 592 796 1101
Fragments arranged by size:
1101 796 592 451
PciI A'CATG_T
Cuts at: 0 2324 2940
Size: 2324 616
PflMI CCAn_nnn'nTGG
Cuts at: 0 508 1084 1286 1420 2940
Size: 508 576 202 134 1520
Fragments arranged by size:
1520 576 508 202 134
PfoI T'CCnGG_A
Cuts at: 0 23 399 1052 1100 2150 2558 2940
Size: 23 376 653 48 1050 408 382
Fragments arranged by size:
1050 653 408 382 376 48 23
PpuMI rG'GwC_Cy
Cuts at: 0 21 202 729 747 766 1674 1731 2940
Size: 21 181 527 18 19 908 57 1209
Fragments arranged by size:
1209 908 527 181 57 21 19 18
PshAI GACnn'nnGTC
Cuts at: 0 518 2940
Size: 518 2422
PspOMI G'GGCC_C
Cuts at: 0 78 447 1407 1550 1724 1817 2230 2940
Size: 78 369 960 143 174 93 413 710
Fragments arranged by size:
960 710 413 369 174 143 93 78
PspXI vC'TCGA_Gb
Cuts at: 0 1771 2940
Size: 1771 1169
PstI C_TGCA'G
Cuts at: 0 129 1535 1572 2060 2132 2385 2858 2940
Size: 129 1406 37 488 72 253 473 82
Fragments arranged by size:
1406 488 473 253 129 82 72 37
PvuII CAG'CTG
Cuts at: 0 1588 1688 2724 2770 2940
Size: 1588 100 1036 46 170
Fragments arranged by size:
1588 1036 170 100 46
RsrII CG'GwC_CG
Cuts at: 0 349 403 2940
Size: 349 54 2537
Fragments arranged by size:
2537 349 54
SacI G_AGCT'C
Cuts at: 0 479 872 1772 2318 2940
Size: 479 393 900 546 622
Fragments arranged by size:
900 622 546 479 393
SacII CC_GC'GG
Cuts at: 0 409 483 2940
Size: 409 74 2457
Fragments arranged by size:
2457 409 74
SanDI GG'GwC_CC
Cuts at: 0 202 766 2940
Size: 202 564 2174
Fragments arranged by size:
2174 564 202
SapI GCTCTTCn'nnn_
Cuts at: 0 188 2940
Size: 188 2752
SbfI CC_TGCA'GG
Cuts at: 0 129 2940
Size: 129 2811
SexAI A'CCwGG_T
Cuts at: 0 2814 2940
Size: 2814 126
SfcI C'TryA_G
Cuts at: 0 125 990 1449 1531 1568 2056 2128 2381
Size: 125 865 459 82 37 488 72 253
Cuts at: 2381 2854 2940
Size: 473 86
Fragments arranged by size:
865 488 473 459 253 125 86 82
72 37
SmaI CCC'GGG
Cuts at: 0 75 207 1549 2940
Size: 75 132 1342 1391
Fragments arranged by size:
1391 1342 132 75
SmlI C'TyrA_G
Cuts at: 0 1771 2940
Size: 1771 1169
StuI AGG'CCT
Cuts at: 0 123 1310 1318 2216 2940
Size: 123 1187 8 898 724
Fragments arranged by size:
1187 898 724 123 8
StyI C'CwwG_G
Cuts at: 0 642 1258 1319 1458 2226 2261 2840 2940
Size: 642 616 61 139 768 35 579 100
Fragments arranged by size:
768 642 616 579 139 100 61 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 55 261 654 660 1228 1612 2110 2940
Size: 55 206 393 6 568 384 498 830
Fragments arranged by size:
830 568 498 393 384 206 55 6
TatI w'GTAC_w
Cuts at: 0 1226 2940
Size: 1226 1714
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 395 427 2940
Size: 395 32 2513
Fragments arranged by size:
2513 395 32
Tth111I GACn'n_nGTC
Cuts at: 0 497 2940
Size: 497 2443
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1197 2233 2940
Size: 1197 1036 707
Fragments arranged by size:
1197 1036 707
XhoI C'TCGA_G
Cuts at: 0 1771 2940
Size: 1771 1169
XmaI C'CCGG_G
Cuts at: 0 73 205 1547 2940
Size: 73 132 1342 1393
Fragments arranged by size:
1393 1342 132 73
Enzymes that do cut:
AarI AcuI AflIII AhdI AleI AlfI AlwNI ApaI
ApoI AvaI BanI BanII BbsI BbvCI BcgI BfrBI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BsaI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI
BssHII BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NheI
NsiI NspI PasI PciI PflMI PfoI PpuMI PshAI
PspOMI PspXI PstI PvuII RsrII SacI SacII SanDI
SapI SbfI SexAI SfcI SmaI SmlI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BciVI BclI BmgBI BpuEI BsaAI BsaBI BsaHI BsiWI
BsmI BspEI BsrBI BssSI BstBI BstEII BstZ17I ClaI
DrdI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI NarI NdeI NotI NruI PacI
PmeI PmlI PpiI PsiI PsrI PvuI SalI ScaI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI XbaI XmnI ZraI