Restriction map for
pFN21AB8536
(Linear) (Six-base) MAPSORT of: KIBB8536.seq Check: 9280 from: 1 to: 2685
KIBB8536.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 1000 1502 2685
Size: 652 348 502 1183
Fragments arranged by size:
1183 652 502 348
AflII C'TTAA_G
Cuts at: 0 1572 1776 2685
Size: 1572 204 909
Fragments arranged by size:
1572 909 204
AflIII A'CryG_T
Cuts at: 0 447 1094 1470 2120 2599 2685
Size: 447 647 376 650 479 86
Fragments arranged by size:
650 647 479 447 376 86
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1916 1948 2685
Size: 1916 32 737
Fragments arranged by size:
1916 737 32
AlwNI CAG_nnn'CTG
Cuts at: 0 45 874 1023 2373 2685
Size: 45 829 149 1350 312
Fragments arranged by size:
1350 829 312 149 45
ApaI G_GGCC'C
Cuts at: 0 2522 2685
Size: 2522 163
ApoI r'AATT_y
Cuts at: 0 201 546 731 843 1741 2436 2662 2685
Size: 201 345 185 112 898 695 226 23
Fragments arranged by size:
898 695 345 226 201 185 112 23
AseI AT'TA_AT
Cuts at: 0 1379 2685
Size: 1379 1306
AvaI C'yCGr_G
Cuts at: 0 620 2685
Size: 620 2065
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1163 1196 1545 1578 2685
Size: 1163 33 349 33 1107
Fragments arranged by size:
1163 1107 349 33 33
BamHI G'GATC_C
Cuts at: 0 773 2685
Size: 773 1912
BanI G'GyrC_C
Cuts at: 0 2525 2685
Size: 2525 160
BanII G_rGCy'C
Cuts at: 0 621 2387 2522 2685
Size: 621 1766 135 163
Fragments arranged by size:
1766 621 163 135
BbsI GAAGACnn'nnnn_
Cuts at: 0 1111 2679 2685
Size: 1111 1568 6
Fragments arranged by size:
1568 1111 6
BciVI GTATCCnnnnn_n'
Cuts at: 0 1164 2685
Size: 1164 1521
BclI T'GATC_A
Cuts at: 0 2129 2685
Size: 2129 556
BglI GCCn_nnn'nGGC
Cuts at: 0 385 2685
Size: 385 2300
BglII A'GATC_T
Cuts at: 0 1835 2685
Size: 1835 850
BlpI GC'TnA_GC
Cuts at: 0 2021 2622 2685
Size: 2021 601 63
Fragments arranged by size:
2021 601 63
Bme1580I G_kGCm'C
Cuts at: 0 828 2269 2522 2685
Size: 828 1441 253 163
Fragments arranged by size:
1441 828 253 163
BmrI ACTGGGnnnn_n'
Cuts at: 0 2524 2685
Size: 2524 161
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2401 2685
Size: 2401 284
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 493 859 1918 2685
Size: 100 393 366 1059 767
Fragments arranged by size:
1059 767 393 366 100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 2685
Size: 292 2393
BsaI GGTCTCn'nnnn_
Cuts at: 0 1106 1571 2685
Size: 1106 465 1114
Fragments arranged by size:
1114 1106 465
BsaBI GATnn'nnATC
Cuts at: 0 1808 2685
Size: 1808 877
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 892 2393 2685
Size: 892 1501 292
Fragments arranged by size:
1501 892 292
BseYI C'CCAG_C
Cuts at: 0 2515 2685
Size: 2515 170
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1370 2624 2685
Size: 1370 1254 61
Fragments arranged by size:
1370 1254 61
BsiHKAI G_wGCw'C
Cuts at: 0 2387 2685
Size: 2387 298
BsmI GAATG_Cn'
Cuts at: 0 654 681 744 997 1949 2451 2685
Size: 654 27 63 253 952 502 234
Fragments arranged by size:
952 654 502 253 234 63 27
Bsp1286I G_dGCh'C
Cuts at: 0 621 828 2269 2387 2522 2685
Size: 621 207 1441 118 135 163
Fragments arranged by size:
1441 621 207 163 135 118
BspHI T'CATG_A
Cuts at: 0 1254 1851 1871 2132 2654 2685
Size: 1254 597 20 261 522 31
Fragments arranged by size:
1254 597 522 261 31 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 39 2685
Size: 39 2646
BsrBI CCG'CTC
Cuts at: 0 978 2685
Size: 978 1707
BsrDI GCAATG_nn'
Cuts at: 0 2477 2644 2685
Size: 2477 167 41
Fragments arranged by size:
2477 167 41
BsrGI T'GTAC_A
Cuts at: 0 1442 2685
Size: 1442 1243
BssSI C'ACGA_G
Cuts at: 0 1927 2685
Size: 1927 758
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1291 1639 2685
Size: 1291 348 1046
Fragments arranged by size:
1291 1046 348
BstBI TT'CG_AA
Cuts at: 0 154 2685
Size: 154 2531
BstEII G'GTnAC_C
Cuts at: 0 99 2685
Size: 99 2586
BstXI CCAn_nnnn'nTGG
Cuts at: 0 529 1353 2685
Size: 529 824 1332
Fragments arranged by size:
1332 824 529
BstYI r'GATC_y
Cuts at: 0 42 773 1020 1835 2271 2363 2685
Size: 42 731 247 815 436 92 322
Fragments arranged by size:
815 731 436 322 247 92 42
Bsu36I CC'TnA_GG
Cuts at: 0 38 803 1997 2685
Size: 38 765 1194 688
Fragments arranged by size:
1194 765 688 38
BtgI C'CryG_G
Cuts at: 0 65 1433 2685
Size: 65 1368 1252
Fragments arranged by size:
1368 1252 65
ClaI AT'CG_AT
Cuts at: 0 32 2685
Size: 32 2653
DraI TTT'AAA
Cuts at: 0 248 2685
Size: 248 2437
DrdI GACnn_nn'nnGTC
Cuts at: 0 474 1412 2685
Size: 474 938 1273
Fragments arranged by size:
1273 938 474
EaeI y'GGCC_r
Cuts at: 0 1215 2111 2685
Size: 1215 896 574
Fragments arranged by size:
1215 896 574
EarI CTCTTCn'nnn_
Cuts at: 0 1019 1913 2685
Size: 1019 894 772
Fragments arranged by size:
1019 894 772
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 958 2685
Size: 958 1727
EcoICRI GAG'CTC
Cuts at: 0 2385 2685
Size: 2385 300
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 493 652 859 1000 1502 1918 2685
Size: 100 393 159 207 141 502 416 767
Fragments arranged by size:
767 502 416 393 207 159 141 100
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1918 2685
Size: 1918 767
EcoO109I rG'GnC_Cy
Cuts at: 0 2519 2685
Size: 2519 166
EcoRV GAT'ATC
Cuts at: 0 1857 2213 2685
Size: 1857 356 472
Fragments arranged by size:
1857 472 356
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 152 184 392 424 571 603 1138 1167
Size: 152 32 208 32 147 32 535 29
Cuts at: 1167 1170 1199 1853 1885 2244 2276 2372 2404
Size: 3 29 654 32 359 32 96 32
Cuts at: 2404 2685
Size: 281
Fragments arranged by size:
654 535 359 281 208 152 147 96
32 32 32 32 32 32 29 29
3
HincII GTy'rAC
Cuts at: 0 1307 1554 1713 2685
Size: 1307 247 159 972
Fragments arranged by size:
1307 972 247 159
HindIII A'AGCT_T
Cuts at: 0 526 2685
Size: 526 2159
HpaI GTT'AAC
Cuts at: 0 1713 2685
Size: 1713 972
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1781 2685
Size: 1781 904
MscI TGG'CCA
Cuts at: 0 2113 2685
Size: 2113 572
MslI CAynn'nnrTG
Cuts at: 0 686 1438 1469 2125 2685
Size: 686 752 31 656 560
Fragments arranged by size:
752 686 656 560 31
MspA1I CmG'CkG
Cuts at: 0 7 313 2608 2685
Size: 7 306 2295 77
Fragments arranged by size:
2295 306 77 7
NcoI C'CATG_G
Cuts at: 0 1433 2685
Size: 1433 1252
NspI r_CATG'y
Cuts at: 0 451 1098 1474 2124 2685
Size: 451 647 376 650 561
Fragments arranged by size:
650 647 561 451 376
PasI CC'CwG_GG
Cuts at: 0 816 2685
Size: 816 1869
PciI A'CATG_T
Cuts at: 0 447 1094 1470 2120 2685
Size: 447 647 376 650 565
Fragments arranged by size:
650 647 565 447 376
PflMI CCAn_nnn'nTGG
Cuts at: 0 1542 2373 2685
Size: 1542 831 312
Fragments arranged by size:
1542 831 312
PfoI T'CCnGG_A
Cuts at: 0 1166 2685
Size: 1166 1519
PspOMI G'GGCC_C
Cuts at: 0 2518 2685
Size: 2518 167
PstI C_TGCA'G
Cuts at: 0 50 2347 2685
Size: 50 2297 338
Fragments arranged by size:
2297 338 50
PvuII CAG'CTG
Cuts at: 0 7 313 2608 2685
Size: 7 306 2295 77
Fragments arranged by size:
2295 306 77 7
SacI G_AGCT'C
Cuts at: 0 2387 2685
Size: 2387 298
SapI GCTCTTCn'nnn_
Cuts at: 0 1019 2685
Size: 1019 1666
ScaI AGT'ACT
Cuts at: 0 1728 2685
Size: 1728 957
SfcI C'TryA_G
Cuts at: 0 46 239 2343 2450 2685
Size: 46 193 2104 107 235
Fragments arranged by size:
2104 235 193 107 46
SmlI C'TyrA_G
Cuts at: 0 271 1572 1776 2685
Size: 271 1301 204 909
Fragments arranged by size:
1301 909 271 204
SspI AAT'ATT
Cuts at: 0 1080 2685
Size: 1080 1605
StyI C'CwwG_G
Cuts at: 0 1433 1766 2685
Size: 1433 333 919
Fragments arranged by size:
1433 919 333
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1972 2543 2685
Size: 1972 571 142
Fragments arranged by size:
1972 571 142
TatI w'GTAC_w
Cuts at: 0 1034 1442 1726 2685
Size: 1034 408 284 959
Fragments arranged by size:
1034 959 408 284
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 758 790 1348 1380 2685
Size: 758 32 558 32 1305
Fragments arranged by size:
1305 758 558 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2407 2685
Size: 2407 278
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2512 2537 2685
Size: 2512 25 148
Fragments arranged by size:
2512 148 25
XmnI GAAnn'nnTTC
Cuts at: 0 731 2685
Size: 731 1954
Enzymes that do cut:
AcuI AflII AflIII AloI AlwNI ApaI ApoI AseI
AvaI BaeI BamHI BanI BanII BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaBI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI ClaI DraI DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
Hin4I HincII HindIII HpaI MmeI MscI MslI MspA1I
NcoI NspI PasI PciI PflMI PfoI PspOMI PstI
PvuII SacI SapI ScaI SfcI SmlI SspI StyI
TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlfI ApaLI AscI AsiSI AvrII BbeI BbvCI
BcgI BfrBI BmgBI BmtI Bpu10I BsaAI BsaHI BsaWI
BsaXI BsiEI BsiWI BsmBI BspEI BsrFI BssHII BstZ17I
BtgZI BtsI CspCI DraIII EagI EcoRI FalI FseI
FspI FspAI HaeII KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI XbaI XhoI XmaI ZraI