Restriction map for
pFN21AB8529
(Linear) (Six-base) MAPSORT of: KIBB8529.seq Check: 7183 from: 1 to: 2889
KIBB8529.orf
With 182 enzymes: SgfI *
December 5, 2014 12:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2277 2889
Size: 2277 612
AccI GT'mk_AC
Cuts at: 0 2143 2889
Size: 2143 746
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 670 1256 2656 2765 2889
Size: 384 286 586 1400 109 124
Fragments arranged by size:
1400 586 384 286 124 109
AflIII A'CryG_T
Cuts at: 0 1118 2031 2889
Size: 1118 913 858
Fragments arranged by size:
1118 913 858
AhdI GACnn_n'nnGTC
Cuts at: 0 2139 2889
Size: 2139 750
AlwNI CAG_nnn'CTG
Cuts at: 0 625 2567 2889
Size: 625 1942 322
Fragments arranged by size:
1942 625 322
ApoI r'AATT_y
Cuts at: 0 661 1266 1314 1814 1833 2094 2338 2881
Size: 661 605 48 500 19 261 244 543
Cuts at: 2881 2889
Size: 8
Fragments arranged by size:
661 605 543 500 261 244 48 19
8
AseI AT'TA_AT
Cuts at: 0 1307 2889
Size: 1307 1582
BanI G'GyrC_C
Cuts at: 0 94 2889
Size: 94 2795
BanII G_rGCy'C
Cuts at: 0 2358 2889
Size: 2358 531
BbvCI CC'TCA_GC
Cuts at: 0 1144 2889
Size: 1144 1745
BciVI GTATCCnnnnn_n'
Cuts at: 0 13 2524 2889
Size: 13 2511 365
Fragments arranged by size:
2511 365 13
BglII A'GATC_T
Cuts at: 0 366 2889
Size: 366 2523
Bme1580I G_kGCm'C
Cuts at: 0 236 2889
Size: 236 2653
BmrI ACTGGGnnnn_n'
Cuts at: 0 977 2577 2602 2889
Size: 977 1600 25 287
Fragments arranged by size:
1600 977 287 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 560 1625 2185 2544 2889
Size: 455 105 1065 560 359 345
Fragments arranged by size:
1065 560 455 359 345 105
Bpu10I CC'TnA_GC
Cuts at: 0 1144 2889
Size: 1144 1745
BsaHI Gr'CG_yC
Cuts at: 0 5 2889
Size: 5 2884
BsaWI w'CCGG_w
Cuts at: 0 1125 2109 2713 2889
Size: 1125 984 604 176
Fragments arranged by size:
1125 984 604 176
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2231 2261 2889
Size: 2231 30 628
Fragments arranged by size:
2231 628 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 799 2889
Size: 799 2090
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 2889
Size: 502 2387
BsiHKAI G_wGCw'C
Cuts at: 0 2358 2889
Size: 2358 531
BsmI GAATG_Cn'
Cuts at: 0 426 2889
Size: 426 2463
Bsp1286I G_dGCh'C
Cuts at: 0 236 2358 2889
Size: 236 2122 531
Fragments arranged by size:
2122 531 236
BspEI T'CCGG_A
Cuts at: 0 1125 2889
Size: 1125 1764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 455 526 1561 1988 2277 2889
Size: 455 71 1035 427 289 612
Fragments arranged by size:
1035 612 455 427 289 71
BsrDI GCAATG_nn'
Cuts at: 0 228 290 1514 2889
Size: 228 62 1224 1375
Fragments arranged by size:
1375 1224 228 62
BsrFI r'CCGG_y
Cuts at: 0 1963 2889
Size: 1963 926
BssSI C'ACGA_G
Cuts at: 0 2333 2889
Size: 2333 556
BstAPI GCAn_nnn'nTGC
Cuts at: 0 621 2889
Size: 621 2268
BstBI TT'CG_AA
Cuts at: 0 1254 2889
Size: 1254 1635
BstEII G'GTnAC_C
Cuts at: 0 442 1803 2889
Size: 442 1361 1086
Fragments arranged by size:
1361 1086 442
BstYI r'GATC_y
Cuts at: 0 366 526 2889
Size: 366 160 2363
Fragments arranged by size:
2363 366 160
Bsu36I CC'TnA_GG
Cuts at: 0 1027 1352 2889
Size: 1027 325 1537
Fragments arranged by size:
1537 1027 325
BtgI C'CryG_G
Cuts at: 0 2128 2889
Size: 2128 761
BtsI GCAGTG_nn'
Cuts at: 0 320 734 1653 2889
Size: 320 414 919 1236
Fragments arranged by size:
1236 919 414 320
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1724 1759 2889
Size: 1724 35 1130
Fragments arranged by size:
1724 1130 35
DraI TTT'AAA
Cuts at: 0 1422 1778 2052 2889
Size: 1422 356 274 837
Fragments arranged by size:
1422 837 356 274
DrdI GACnn_nn'nnGTC
Cuts at: 0 826 2889
Size: 826 2063
EarI CTCTTCn'nnn_
Cuts at: 0 817 2355 2889
Size: 817 1538 534
Fragments arranged by size:
1538 817 534
EcoICRI GAG'CTC
Cuts at: 0 2356 2889
Size: 2356 533
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 455 560 670 1256 1625 2185 2544
Size: 384 71 105 110 586 369 560 359
Cuts at: 2544 2656 2765 2889
Size: 112 109 124
Fragments arranged by size:
586 560 384 369 359 124 112 110
109 105 71
EcoRI G'AATT_C
Cuts at: 0 661 1266 1314 2094 2338 2889
Size: 661 605 48 780 244 551
Fragments arranged by size:
780 661 605 551 244 48
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 90 1864 2408 2889
Size: 90 1774 544 481
Fragments arranged by size:
1774 544 481 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1447 1479 1496 1528 1700 1732 2125 2157
Size: 1447 32 17 32 172 32 393 32
Cuts at: 2157 2231 2248 2263 2280 2889
Size: 74 17 15 17 609
Fragments arranged by size:
1447 609 393 172 74 32 32 32
32 17 17 17 15
HincII GTy'rAC
Cuts at: 0 378 965 1161 2889
Size: 378 587 196 1728
Fragments arranged by size:
1728 587 378 196
HindIII A'AGCT_T
Cuts at: 0 493 1702 2407 2889
Size: 493 1209 705 482
Fragments arranged by size:
1209 705 493 482
HpaI GTT'AAC
Cuts at: 0 965 2889
Size: 965 1924
MfeI C'AATT_G
Cuts at: 0 2192 2889
Size: 2192 697
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 385 867 933 1395 2889
Size: 116 269 482 66 462 1494
Fragments arranged by size:
1494 482 462 269 116 66
MslI CAynn'nnrTG
Cuts at: 0 850 2889
Size: 850 2039
MspA1I CmG'CkG
Cuts at: 0 1441 2889
Size: 1441 1448
NcoI C'CATG_G
Cuts at: 0 2128 2889
Size: 2128 761
NspI r_CATG'y
Cuts at: 0 1122 2035 2889
Size: 1122 913 854
Fragments arranged by size:
1122 913 854
PciI A'CATG_T
Cuts at: 0 1118 2031 2889
Size: 1118 913 858
Fragments arranged by size:
1118 913 858
PflMI CCAn_nnn'nTGG
Cuts at: 0 433 1648 2567 2889
Size: 433 1215 919 322
Fragments arranged by size:
1215 919 433 322
PfoI T'CCnGG_A
Cuts at: 0 432 738 2682 2757 2889
Size: 432 306 1944 75 132
Fragments arranged by size:
1944 432 306 132 75
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1994 2026 2889
Size: 1994 32 863
Fragments arranged by size:
1994 863 32
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2021 2053 2889
Size: 2021 32 836
Fragments arranged by size:
2021 836 32
PstI C_TGCA'G
Cuts at: 0 452 505 1524 1572 2274 2493 2889
Size: 452 53 1019 48 702 219 396
Fragments arranged by size:
1019 702 452 396 219 53 48
PvuII CAG'CTG
Cuts at: 0 1441 2889
Size: 1441 1448
SacI G_AGCT'C
Cuts at: 0 2358 2889
Size: 2358 531
SbfI CC_TGCA'GG
Cuts at: 0 2274 2889
Size: 2274 615
SfcI C'TryA_G
Cuts at: 0 448 501 1091 1520 1568 2270 2489 2889
Size: 448 53 590 429 48 702 219 400
Fragments arranged by size:
702 590 448 429 400 219 53 48
SspI AAT'ATT
Cuts at: 0 775 2889
Size: 775 2114
StuI AGG'CCT
Cuts at: 0 2324 2889
Size: 2324 565
StyI C'CwwG_G
Cuts at: 0 2128 2702 2889
Size: 2128 574 187
Fragments arranged by size:
2128 574 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 606 2584 2889
Size: 606 1978 305
Fragments arranged by size:
1978 606 305
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2660 2692 2889
Size: 2660 32 197
Fragments arranged by size:
2660 197 32
Tth111I GACn'n_nGTC
Cuts at: 0 2245 2889
Size: 2245 644
XmnI GAAnn'nnTTC
Cuts at: 0 73 567 2009 2889
Size: 73 494 1442 880
Fragments arranged by size:
1442 880 494 73
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AlwNI ApoI AseI
BanI BanII BbvCI BciVI BglII Bme1580I BmrI BpmI
Bpu10I BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI
Bsp1286I BspEI BspMI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtsI CspCI DraI DrdI
EarI EcoICRI Eco57MI EcoRI FalI Hin4I HincII HindIII
HpaI MfeI MmeI MslI MspA1I NcoI NspI PciI
PflMI PfoI PpiI PsrI PstI PvuII SacI SbfI
SfcI SspI StuI StyI TaqII TstI Tth111I XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI AlfI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BbeI BbsI BcgI BclI BfrBI BglI BlpI
BmgBI BmtI BplI BpuEI BsaI BsaAI BsaBI BseYI
BsiEI BsiWI BsmBI BspHI BsrBI BsrGI BssHII BstXI
BstZ17I BtgZI ClaI DraIII EaeI EagI EciI EcoNI
EcoO109I EcoRV FseI FspI FspAI HaeII KasI KpnI
MluI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PmeI PmlI PpuMI PshAI
PsiI PspOMI PspXI PvuI RsrII SacII SalI SanDI
SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SwaI TatI XbaI
XcmI XhoI XmaI ZraI