Restriction map for
pFN21AB8516
(Linear) (Six-base) MAPSORT of: KIBB8516.seq Check: 8850 from: 1 to: 2613
KIBB8516.orf
With 182 enzymes: SgfI *
July 15, 2010 18:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2312 2613
Size: 2312 301
AccI GT'mk_AC
Cuts at: 0 1859 2613
Size: 1859 754
Acc65I G'GTAC_C
Cuts at: 0 2475 2613
Size: 2475 138
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1807 1871 1980 2316 2613
Size: 1807 64 109 336 297
Fragments arranged by size:
1807 336 297 109 64
AfeI AGC'GCT
Cuts at: 0 2236 2613
Size: 2236 377
AflIII A'CryG_T
Cuts at: 0 1274 1276 2613
Size: 1274 2 1337
Fragments arranged by size:
1337 1274 2
AhdI GACnn_n'nnGTC
Cuts at: 0 471 2613
Size: 471 2142
AleI CACnn'nnGTG
Cuts at: 0 350 1513 2119 2613
Size: 350 1163 606 494
Fragments arranged by size:
1163 606 494 350
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 250 985 2613
Size: 250 735 1628
Fragments arranged by size:
1628 735 250
AlwNI CAG_nnn'CTG
Cuts at: 0 1479 2613
Size: 1479 1134
ApaI G_GGCC'C
Cuts at: 0 35 296 2613
Size: 35 261 2317
Fragments arranged by size:
2317 261 35
ApoI r'AATT_y
Cuts at: 0 1620 2613
Size: 1620 993
AvaI C'yCGr_G
Cuts at: 0 34 55 476 2394 2613
Size: 34 21 421 1918 219
Fragments arranged by size:
1918 421 219 34 21
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1480 1513 2613
Size: 1480 33 1100
Fragments arranged by size:
1480 1100 33
BanI G'GyrC_C
Cuts at: 0 70 217 234 285 331 944 1199 2475
Size: 70 147 17 51 46 613 255 1276
Cuts at: 2475 2526 2613
Size: 51 87
Fragments arranged by size:
1276 613 255 147 87 70 51 51
46 17
BanII G_rGCy'C
Cuts at: 0 35 53 296 403 484 1361 2302 2613
Size: 35 18 243 107 81 877 941 311
Fragments arranged by size:
941 877 311 243 107 81 35 18
BbeI G_GCGC'C
Cuts at: 0 221 948 2613
Size: 221 727 1665
Fragments arranged by size:
1665 727 221
BbsI GAAGACnn'nnnn_
Cuts at: 0 435 580 2073 2613
Size: 435 145 1493 540
Fragments arranged by size:
1493 540 435 145
BbvCI CC'TCA_GC
Cuts at: 0 419 2613
Size: 419 2194
BglI GCCn_nnn'nGGC
Cuts at: 0 173 1723 2613
Size: 173 1550 890
Fragments arranged by size:
1550 890 173
BlpI GC'TnA_GC
Cuts at: 0 47 2056 2613
Size: 47 2009 557
Fragments arranged by size:
2009 557 47
Bme1580I G_kGCm'C
Cuts at: 0 35 239 296 1062 1489 1964 2613
Size: 35 204 57 766 427 475 649
Fragments arranged by size:
766 649 475 427 204 57 35
BmrI ACTGGGnnnn_n'
Cuts at: 0 1233 1568 2613
Size: 1233 335 1045
Fragments arranged by size:
1233 1045 335
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 150 2613
Size: 150 2463
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 333 1105 1321 1501 1634 2068 2613
Size: 277 56 772 216 180 133 434 545
Fragments arranged by size:
772 545 434 277 216 180 133 56
Bpu10I CC'TnA_GC
Cuts at: 0 419 604 2613
Size: 419 185 2009
Fragments arranged by size:
2009 419 185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1036 2613
Size: 1036 1577
BsaI GGTCTCn'nnnn_
Cuts at: 0 1431 1902 1984 2178 2360 2401 2613
Size: 1431 471 82 194 182 41 212
Fragments arranged by size:
1431 471 212 194 182 82 41
BsaAI yAC'GTr
Cuts at: 0 759 1277 2613
Size: 759 518 1336
Fragments arranged by size:
1336 759 518
BsaBI GATnn'nnATC
Cuts at: 0 1150 2613
Size: 1150 1463
BsaHI Gr'CG_yC
Cuts at: 0 218 945 2603 2613
Size: 218 727 1658 10
Fragments arranged by size:
1658 727 218 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 773 803 1249 1279 1411 1441 2613
Size: 773 30 446 30 132 30 1172
Fragments arranged by size:
1172 773 446 132 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 90 562 1254 2119 2613
Size: 90 472 692 865 494
Fragments arranged by size:
865 692 494 472 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 864 1938 2613
Size: 709 155 1074 675
Fragments arranged by size:
1074 709 675 155
BsiHKAI G_wGCw'C
Cuts at: 0 53 1097 2302 2613
Size: 53 1044 1205 311
Fragments arranged by size:
1205 1044 311 53
BsmI GAATG_Cn'
Cuts at: 0 1767 2613
Size: 1767 846
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1410 1689 2613
Size: 1410 279 924
Fragments arranged by size:
1410 924 279
Bsp1286I G_dGCh'C
Cuts at: 0 35 53 239 296 403 484 1062 1097
Size: 35 18 186 57 107 81 578 35
Cuts at: 1097 1361 1489 1964 2302 2613
Size: 264 128 475 338 311
Fragments arranged by size:
578 475 338 311 264 186 128 107
81 57 35 35 18
BspHI T'CATG_A
Cuts at: 0 1121 1598 1811 1871 2613
Size: 1121 477 213 60 742
Fragments arranged by size:
1121 742 477 213 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 586 1049 1532 2312 2613
Size: 586 463 483 780 301
Fragments arranged by size:
780 586 483 463 301
BsrBI CCG'CTC
Cuts at: 0 2270 2610 2613
Size: 2270 340 3
Fragments arranged by size:
2270 340 3
BsrDI GCAATG_nn'
Cuts at: 0 615 989 1941 2141 2613
Size: 615 374 952 200 472
Fragments arranged by size:
952 615 472 374 200
BsrFI r'CCGG_y
Cuts at: 0 150 449 658 2613
Size: 150 299 209 1955
Fragments arranged by size:
1955 299 209 150
BsrGI T'GTAC_A
Cuts at: 0 1313 2613
Size: 1313 1300
BssSI C'ACGA_G
Cuts at: 0 769 1339 1974 2613
Size: 769 570 635 639
Fragments arranged by size:
769 639 635 570
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2327 2613
Size: 2327 286
BstEII G'GTnAC_C
Cuts at: 0 2097 2283 2575 2613
Size: 2097 186 292 38
Fragments arranged by size:
2097 292 186 38
BstXI CCAn_nnnn'nTGG
Cuts at: 0 805 2037 2223 2422 2613
Size: 805 1232 186 199 191
Fragments arranged by size:
1232 805 199 191 186
BstYI r'GATC_y
Cuts at: 0 1151 1304 2613
Size: 1151 153 1309
Fragments arranged by size:
1309 1151 153
Bsu36I CC'TnA_GG
Cuts at: 0 2254 2613
Size: 2254 359
BtgI C'CryG_G
Cuts at: 0 308 804 1442 1511 1779 2075 2288 2438
Size: 308 496 638 69 268 296 213 150
Cuts at: 2438 2613
Size: 175
Fragments arranged by size:
638 496 308 296 268 213 175 150
69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 197 630 714 2573 2613
Size: 197 433 84 1859 40
Fragments arranged by size:
1859 433 197 84 40
BtsI GCAGTG_nn'
Cuts at: 0 485 2212 2613
Size: 485 1727 401
Fragments arranged by size:
1727 485 401
ClaI AT'CG_AT
Cuts at: 0 248 2613
Size: 248 2365
DraIII CAC_nnn'GTG
Cuts at: 0 759 2613
Size: 759 1854
EaeI y'GGCC_r
Cuts at: 0 107 612 986 1724 2562 2613
Size: 107 505 374 738 838 51
Fragments arranged by size:
838 738 505 374 107 51
EarI CTCTTCn'nnn_
Cuts at: 0 1072 1280 1558 1633 1834 1873 2613
Size: 1072 208 278 75 201 39 740
Fragments arranged by size:
1072 740 278 208 201 75 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 949 2613
Size: 19 930 1664
Fragments arranged by size:
1664 930 19
EcoICRI GAG'CTC
Cuts at: 0 51 2300 2613
Size: 51 2249 313
Fragments arranged by size:
2249 313 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 333 1105 1321 1501 1634 1807 1871
Size: 277 56 772 216 180 133 173 64
Cuts at: 1871 1980 2068 2316 2613
Size: 109 88 248 297
Fragments arranged by size:
772 297 277 248 216 180 173 133
109 88 64 56
EcoNI CCTnn'n_nnAGG
Cuts at: 0 586 2257 2613
Size: 586 1671 356
Fragments arranged by size:
1671 586 356
EcoO109I rG'GnC_Cy
Cuts at: 0 19 31 1563 1799 1836 2391 2613
Size: 19 12 1532 236 37 555 222
Fragments arranged by size:
1532 555 236 222 37 19 12
EcoRI G'AATT_C
Cuts at: 0 1620 2613
Size: 1620 993
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1845 2613
Size: 1845 768
FspI TGC'GCA
Cuts at: 0 202 2613
Size: 202 2411
HaeII r_GCGC'y
Cuts at: 0 221 813 948 2238 2613
Size: 221 592 135 1290 375
Fragments arranged by size:
1290 592 375 221 135
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 457 489 773 805 1096 1128 1249 1281
Size: 457 32 284 32 291 32 121 32
Cuts at: 1281 1838 1870 2449 2481 2613
Size: 557 32 579 32 132
Fragments arranged by size:
579 557 457 291 284 132 121 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 903 2613
Size: 903 1710
KasI G'GCGC_C
Cuts at: 0 217 944 2613
Size: 217 727 1669
Fragments arranged by size:
1669 727 217
KpnI G_GTAC'C
Cuts at: 0 2479 2613
Size: 2479 134
MscI TGG'CCA
Cuts at: 0 109 988 2564 2613
Size: 109 879 1576 49
Fragments arranged by size:
1576 879 109 49
MslI CAynn'nnrTG
Cuts at: 0 350 631 1420 1513 1927 2035 2119 2613
Size: 350 281 789 93 414 108 84 494
Fragments arranged by size:
789 494 414 350 281 108 93 84
MspA1I CmG'CkG
Cuts at: 0 1479 1650 2555 2613
Size: 1479 171 905 58
Fragments arranged by size:
1479 905 171 58
NarI GG'CG_CC
Cuts at: 0 218 945 2613
Size: 218 727 1668
Fragments arranged by size:
1668 727 218
NcoI C'CATG_G
Cuts at: 0 308 1442 2613
Size: 308 1134 1171
Fragments arranged by size:
1171 1134 308
NruI TCG'CGA
Cuts at: 0 957 2613
Size: 957 1656
NspI r_CATG'y
Cuts at: 0 942 979 2139 2613
Size: 942 37 1160 474
Fragments arranged by size:
1160 942 474 37
PasI CC'CwG_GG
Cuts at: 0 777 2613
Size: 777 1836
PflMI CCAn_nnn'nTGG
Cuts at: 0 184 1479 2373 2613
Size: 184 1295 894 240
Fragments arranged by size:
1295 894 240 184
PfoI T'CCnGG_A
Cuts at: 0 2009 2222 2613
Size: 2009 213 391
Fragments arranged by size:
2009 391 213
PmlI CAC'GTG
Cuts at: 0 1277 2613
Size: 1277 1336
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2488 2520 2613
Size: 2488 32 93
Fragments arranged by size:
2488 93 32
PpuMI rG'GwC_Cy
Cuts at: 0 1563 1799 1836 2391 2613
Size: 1563 236 37 555 222
Fragments arranged by size:
1563 555 236 222 37
PspOMI G'GGCC_C
Cuts at: 0 31 292 2613
Size: 31 261 2321
Fragments arranged by size:
2321 261 31
PstI C_TGCA'G
Cuts at: 0 2207 2613
Size: 2207 406
PvuII CAG'CTG
Cuts at: 0 1650 2613
Size: 1650 963
SacI G_AGCT'C
Cuts at: 0 53 2302 2613
Size: 53 2249 311
Fragments arranged by size:
2249 311 53
SapI GCTCTTCn'nnn_
Cuts at: 0 1558 1873 2613
Size: 1558 315 740
Fragments arranged by size:
1558 740 315
SfcI C'TryA_G
Cuts at: 0 1803 2203 2613
Size: 1803 400 410
Fragments arranged by size:
1803 410 400
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1723 2613
Size: 1723 890
SfoI GGC'GCC
Cuts at: 0 219 946 2613
Size: 219 727 1667
Fragments arranged by size:
1667 727 219
SmaI CCC'GGG
Cuts at: 0 36 57 478 2396 2613
Size: 36 21 421 1918 217
Fragments arranged by size:
1918 421 217 36 21
SmlI C'TyrA_G
Cuts at: 0 1051 2613
Size: 1051 1562
SphI G_CATG'C
Cuts at: 0 979 2613
Size: 979 1634
StuI AGG'CCT
Cuts at: 0 592 1291 1717 2263 2613
Size: 592 699 426 546 350
Fragments arranged by size:
699 592 546 426 350
StyI C'CwwG_G
Cuts at: 0 308 587 824 1442 2177 2613
Size: 308 279 237 618 735 436
Fragments arranged by size:
735 618 436 308 279 237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 321 2613
Size: 321 2292
TatI w'GTAC_w
Cuts at: 0 1313 2613
Size: 1313 1300
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 252 492 2073 2220 2613
Size: 252 240 1581 147 393
Fragments arranged by size:
1581 393 252 240 147
XmaI C'CCGG_G
Cuts at: 0 34 55 476 2394 2613
Size: 34 21 421 1918 219
Fragments arranged by size:
1918 421 219 34 21
XmnI GAAnn'nnTTC
Cuts at: 0 2263 2613
Size: 2263 350
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI
AlfI AlwNI ApaI ApoI AvaI BaeI BanI BanII
BbeI BbsI BbvCI BglI BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MscI MslI
MspA1I NarI NcoI NruI NspI PasI PflMI PfoI
PmlI PpiI PpuMI PspOMI PstI PvuII SacI SapI
SfcI SfiI SfoI SmaI SmlI SphI StuI StyI
TaqII TatI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AloI ApaLI AscI AseI
AsiSI AvrII BamHI BcgI BciVI BclI BfrBI BglII
BmgBI BmtI BsaWI BseYI BsiEI BsiWI BspEI BssHII
BstBI BstZ17I CspCI DraI DrdI EagI EcoRV FseI
FspAI HindIII HpaI MfeI MluI MmeI NaeI NdeI
NgoMIV NheI NotI NsiI PacI PciI PmeI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI TstI Tth111I XbaI XhoI ZraI