Restriction map for
pFN21AB8513
(Linear) (Six-base) MAPSORT of: KIBB8513.seq Check: 8068 from: 1 to: 2829
KIBB8513.orf
With 182 enzymes: SgfI *
July 3, 2012 16:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 315 405 666 2829
Size: 315 90 261 2163
Fragments arranged by size:
2163 315 261 90
AatII G_ACGT'C
Cuts at: 0 2732 2829
Size: 2732 97
AccI GT'mk_AC
Cuts at: 0 2789 2829
Size: 2789 40
Acc65I G'GTAC_C
Cuts at: 0 340 2829
Size: 340 2489
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 1172 2077 2493 2584 2829
Size: 611 561 905 416 91 245
Fragments arranged by size:
905 611 561 416 245 91
AfeI AGC'GCT
Cuts at: 0 2083 2164 2829
Size: 2083 81 665
Fragments arranged by size:
2083 665 81
AflIII A'CryG_T
Cuts at: 0 1552 2294 2829
Size: 1552 742 535
Fragments arranged by size:
1552 742 535
AleI CACnn'nnGTG
Cuts at: 0 904 1564 2829
Size: 904 660 1265
Fragments arranged by size:
1265 904 660
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1582 2829
Size: 1582 1247
AlwNI CAG_nnn'CTG
Cuts at: 0 1038 2718 2829
Size: 1038 1680 111
Fragments arranged by size:
1680 1038 111
ApaI G_GGCC'C
Cuts at: 0 83 659 1079 1242 1281 2829
Size: 83 576 420 163 39 1548
Fragments arranged by size:
1548 576 420 163 83 39
ApaLI G'TGCA_C
Cuts at: 0 1954 2829
Size: 1954 875
ApoI r'AATT_y
Cuts at: 0 1397 2829
Size: 1397 1432
AvaI C'yCGr_G
Cuts at: 0 75 108 148 196 214 837 1757 2323
Size: 75 33 40 48 18 623 920 566
Cuts at: 2323 2769 2829
Size: 446 60
Fragments arranged by size:
920 623 566 446 75 60 48 40
33 18
BamHI G'GATC_C
Cuts at: 0 2000 2051 2829
Size: 2000 51 778
Fragments arranged by size:
2000 778 51
BanI G'GyrC_C
Cuts at: 0 157 340 571 1522 1714 2452 2829
Size: 157 183 231 951 192 738 377
Fragments arranged by size:
951 738 377 231 192 183 157
BanII G_rGCy'C
Cuts at: 0 83 130 206 214 466 507 659 829
Size: 83 47 76 8 252 41 152 170
Cuts at: 829 981 1079 1141 1242 1281 2258 2777 2829
Size: 152 98 62 101 39 977 519 52
Fragments arranged by size:
977 519 252 170 152 152 101 98
83 76 62 52 47 41 39 8
BbeI G_GCGC'C
Cuts at: 0 161 575 2829
Size: 161 414 2254
Fragments arranged by size:
2254 414 161
BbsI GAAGACnn'nnnn_
Cuts at: 0 1835 2453 2482 2829
Size: 1835 618 29 347
Fragments arranged by size:
1835 618 347 29
BbvCI CC'TCA_GC
Cuts at: 0 1362 2113 2829
Size: 1362 751 716
Fragments arranged by size:
1362 751 716
BciVI GTATCCnnnnn_n'
Cuts at: 0 781 2829
Size: 781 2048
BclI T'GATC_A
Cuts at: 0 35 2829
Size: 35 2794
BglI GCCn_nnn'nGGC
Cuts at: 0 218 1543 1760 2829
Size: 218 1325 217 1069
Fragments arranged by size:
1325 1069 218 217
BglII A'GATC_T
Cuts at: 0 1449 2829
Size: 1449 1380
BlpI GC'TnA_GC
Cuts at: 0 124 2829
Size: 124 2705
Bme1580I G_kGCm'C
Cuts at: 0 83 526 659 1079 1242 1281 1471 1525
Size: 83 443 133 420 163 39 190 54
Cuts at: 1525 1717 1958 2829
Size: 192 241 871
Fragments arranged by size:
871 443 420 241 192 190 163 133
83 54 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 77 938 2829
Size: 77 861 1891
Fragments arranged by size:
1891 861 77
BmtI G_CTAG'C
Cuts at: 0 887 2829
Size: 887 1942
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1863 2829
Size: 1863 966
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1438 1491 1833 2266 2829
Size: 1438 53 342 433 563
Fragments arranged by size:
1438 563 433 342 53
Bpu10I CC'TnA_GC
Cuts at: 0 734 1216 1362 1859 2113 2829
Size: 734 482 146 497 254 716
Fragments arranged by size:
734 716 497 482 254 146
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1009 2829
Size: 1009 1820
BsaI GGTCTCn'nnnn_
Cuts at: 0 1967 2386 2829
Size: 1967 419 443
Fragments arranged by size:
1967 443 419
BsaBI GATnn'nnATC
Cuts at: 0 2230 2829
Size: 2230 599
BsaHI Gr'CG_yC
Cuts at: 0 97 158 572 2729 2829
Size: 97 61 414 2157 100
Fragments arranged by size:
2157 414 100 97 61
BsaWI w'CCGG_w
Cuts at: 0 1623 2829
Size: 1623 1206
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 966 996 1312 1342 2829
Size: 966 30 316 30 1487
Fragments arranged by size:
1487 966 316 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 126 214 379 660 1186 1276 1753 1791
Size: 126 88 165 281 526 90 477 38
Cuts at: 1791 1806 1852 2263 2266 2353 2623 2829
Size: 15 46 411 3 87 270 206
Fragments arranged by size:
526 477 411 281 270 206 165 126
90 88 87 46 38 15 3
BseYI C'CCAG_C
Cuts at: 0 177 658 1056 1338 1678 2278 2829
Size: 177 481 398 282 340 600 551
Fragments arranged by size:
600 551 481 398 340 282 177
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 1513 2829
Size: 433 1080 1316
Fragments arranged by size:
1316 1080 433
BsiEI CG_ry'CG
Cuts at: 0 234 708 2829
Size: 234 474 2121
Fragments arranged by size:
2121 474 234
BsiHKAI G_wGCw'C
Cuts at: 0 466 1958 2258 2829
Size: 466 1492 300 571
Fragments arranged by size:
1492 571 466 300
BsmI GAATG_Cn'
Cuts at: 0 695 1418 2829
Size: 695 723 1411
Fragments arranged by size:
1411 723 695
BsmBI CGTCTCn'nnnn_
Cuts at: 0 88 1168 1735 2829
Size: 88 1080 567 1094
Fragments arranged by size:
1094 1080 567 88
Bsp1286I G_dGCh'C
Cuts at: 0 83 130 206 214 466 507 526 659
Size: 83 47 76 8 252 41 19 133
Cuts at: 659 829 981 1079 1141 1242 1281 1471 1525
Size: 170 152 98 62 101 39 190 54
Cuts at: 1525 1717 1958 2258 2777 2829
Size: 192 241 300 519 52
Fragments arranged by size:
519 300 252 241 192 190 170 152
133 101 98 83 76 62 54 52
47 41 39 19 8
BspEI T'CCGG_A
Cuts at: 0 1623 2829
Size: 1623 1206
BspHI T'CATG_A
Cuts at: 0 38 2829
Size: 38 2791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 315 405 666 1004 1698 1775 2136 2192
Size: 315 90 261 338 694 77 361 56
Cuts at: 2192 2484 2829
Size: 292 345
Fragments arranged by size:
694 361 345 338 315 292 261 90
77 56
BsrBI CCG'CTC
Cuts at: 0 65 230 2829
Size: 65 165 2599
Fragments arranged by size:
2599 165 65
BsrDI GCAATG_nn'
Cuts at: 0 619 2829
Size: 619 2210
BsrFI r'CCGG_y
Cuts at: 0 233 317 337 1537 2093 2558 2829
Size: 233 84 20 1200 556 465 271
Fragments arranged by size:
1200 556 465 271 233 84 20
BssSI C'ACGA_G
Cuts at: 0 2611 2829
Size: 2611 218
BstAPI GCAn_nnn'nTGC
Cuts at: 0 411 2829
Size: 411 2418
BstBI TT'CG_AA
Cuts at: 0 2574 2829
Size: 2574 255
BstEII G'GTnAC_C
Cuts at: 0 2496 2829
Size: 2496 333
BstXI CCAn_nnnn'nTGG
Cuts at: 0 807 906 1242 1726 2829
Size: 807 99 336 484 1103
Fragments arranged by size:
1103 807 484 336 99
BstYI r'GATC_y
Cuts at: 0 386 948 1449 2000 2051 2829
Size: 386 562 501 551 51 778
Fragments arranged by size:
778 562 551 501 386 51
Bsu36I CC'TnA_GG
Cuts at: 0 1259 1660 1774 2705 2829
Size: 1259 401 114 931 124
Fragments arranged by size:
1259 931 401 124 114
BtgI C'CryG_G
Cuts at: 0 899 1283 1526 1805 2136 2371 2405 2829
Size: 899 384 243 279 331 235 34 424
Fragments arranged by size:
899 424 384 331 279 243 235 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1097 2829
Size: 1097 1732
BtsI GCAGTG_nn'
Cuts at: 0 125 267 1050 2389 2829
Size: 125 142 783 1339 440
Fragments arranged by size:
1339 783 440 142 125
DrdI GACnn_nn'nnGTC
Cuts at: 0 2805 2829
Size: 2805 24
EaeI y'GGCC_r
Cuts at: 0 231 705 896 2189 2408 2829
Size: 231 474 191 1293 219 421
Fragments arranged by size:
1293 474 421 231 219 191
EagI C'GGCC_G
Cuts at: 0 231 705 2829
Size: 231 474 2124
Fragments arranged by size:
2124 474 231
EarI CTCTTCn'nnn_
Cuts at: 0 55 175 704 1794 2641 2829
Size: 55 120 529 1090 847 188
Fragments arranged by size:
1090 847 529 188 120 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 240 456 2829
Size: 240 216 2373
Fragments arranged by size:
2373 240 216
EcoICRI GAG'CTC
Cuts at: 0 464 2256 2829
Size: 464 1792 573
Fragments arranged by size:
1792 573 464
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 1172 1438 1491 1833 2077 2266 2493
Size: 611 561 266 53 342 244 189 227
Cuts at: 2493 2584 2829
Size: 91 245
Fragments arranged by size:
611 561 342 266 245 244 227 189
91 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 565 1663 2248 2287 2704 2829
Size: 565 1098 585 39 417 125
Fragments arranged by size:
1098 585 565 417 125 39
EcoO109I rG'GnC_Cy
Cuts at: 0 79 558 655 993 1011 1076 1238 1239
Size: 79 479 97 338 18 65 162 1
Cuts at: 1239 1255 1277 1278 1328 1730 2117 2237 2328
Size: 16 22 1 50 402 387 120 91
Cuts at: 2328 2829
Size: 501
Fragments arranged by size:
501 479 402 387 338 162 120 97
91 79 65 50 22 18 16 1
1
EcoRI G'AATT_C
Cuts at: 0 1397 2829
Size: 1397 1432
EcoRV GAT'ATC
Cuts at: 0 1117 2829
Size: 1117 1712
HaeII r_GCGC'y
Cuts at: 0 50 161 481 575 2085 2166 2829
Size: 50 111 320 94 1510 81 663
Fragments arranged by size:
1510 663 320 111 94 81 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 175 207 1312 1344 1864 1896 2185 2217
Size: 175 32 1105 32 520 32 289 32
Cuts at: 2217 2535 2567 2829
Size: 318 32 262
Fragments arranged by size:
1105 520 318 289 262 175 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 2790 2829
Size: 2790 39
KasI G'GCGC_C
Cuts at: 0 157 571 2829
Size: 157 414 2258
Fragments arranged by size:
2258 414 157
KpnI G_GTAC'C
Cuts at: 0 344 2829
Size: 344 2485
MluI A'CGCG_T
Cuts at: 0 1552 2829
Size: 1552 1277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1334 2829
Size: 1334 1495
MscI TGG'CCA
Cuts at: 0 898 2829
Size: 898 1931
MslI CAynn'nnrTG
Cuts at: 0 805 904 1564 2141 2829
Size: 805 99 660 577 688
Fragments arranged by size:
805 688 660 577 99
MspA1I CmG'CkG
Cuts at: 0 257 269 788 1596 2007 2038 2355 2829
Size: 257 12 519 808 411 31 317 474
Fragments arranged by size:
808 519 474 411 317 257 31 12
NaeI GCC'GGC
Cuts at: 0 235 2829
Size: 235 2594
NarI GG'CG_CC
Cuts at: 0 158 572 2829
Size: 158 414 2257
Fragments arranged by size:
2257 414 158
NcoI C'CATG_G
Cuts at: 0 1526 2136 2829
Size: 1526 610 693
Fragments arranged by size:
1526 693 610
NgoMIV G'CCGG_C
Cuts at: 0 233 2829
Size: 233 2596
NheI G'CTAG_C
Cuts at: 0 883 2829
Size: 883 1946
NspI r_CATG'y
Cuts at: 0 438 1134 2298 2382 2829
Size: 438 696 1164 84 447
Fragments arranged by size:
1164 696 447 438 84
PasI CC'CwG_GG
Cuts at: 0 1096 1235 2415 2829
Size: 1096 139 1180 414
Fragments arranged by size:
1180 1096 414 139
PciI A'CATG_T
Cuts at: 0 2294 2829
Size: 2294 535
PflMI CCAn_nnn'nTGG
Cuts at: 0 429 1514 2669 2829
Size: 429 1085 1155 160
Fragments arranged by size:
1155 1085 429 160
PfoI T'CCnGG_A
Cuts at: 0 640 2829
Size: 640 2189
PpuMI rG'GwC_Cy
Cuts at: 0 558 993 1328 2237 2829
Size: 558 435 335 909 592
Fragments arranged by size:
909 592 558 435 335
PspOMI G'GGCC_C
Cuts at: 0 79 655 1075 1238 1277 2829
Size: 79 576 420 163 39 1552
Fragments arranged by size:
1552 576 420 163 79 39
PstI C_TGCA'G
Cuts at: 0 1001 1675 2723 2829
Size: 1001 674 1048 106
Fragments arranged by size:
1048 1001 674 106
PvuII CAG'CTG
Cuts at: 0 2007 2038 2829
Size: 2007 31 791
Fragments arranged by size:
2007 791 31
SacI G_AGCT'C
Cuts at: 0 466 2258 2829
Size: 466 1792 571
Fragments arranged by size:
1792 571 466
SalI G'TCGA_C
Cuts at: 0 2788 2829
Size: 2788 41
SanDI GG'GwC_CC
Cuts at: 0 1328 2829
Size: 1328 1501
SapI GCTCTTCn'nnn_
Cuts at: 0 55 2829
Size: 55 2774
SfcI C'TryA_G
Cuts at: 0 719 997 1671 2719 2829
Size: 719 278 674 1048 110
Fragments arranged by size:
1048 719 674 278 110
SfoI GGC'GCC
Cuts at: 0 159 573 2829
Size: 159 414 2256
Fragments arranged by size:
2256 414 159
SmaI CCC'GGG
Cuts at: 0 77 150 216 839 2829
Size: 77 73 66 623 1990
Fragments arranged by size:
1990 623 77 73 66
SmlI C'TyrA_G
Cuts at: 0 988 2829
Size: 988 1841
SphI G_CATG'C
Cuts at: 0 1134 2829
Size: 1134 1695
StuI AGG'CCT
Cuts at: 0 1669 2506 2829
Size: 1669 837 323
Fragments arranged by size:
1669 837 323
StyI C'CwwG_G
Cuts at: 0 1526 1733 2136 2829
Size: 1526 207 403 693
Fragments arranged by size:
1526 693 403 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 813 1477 1732 2815 2829
Size: 813 664 255 1083 14
Fragments arranged by size:
1083 813 664 255 14
TatI w'GTAC_w
Cuts at: 0 8 2829
Size: 8 2821
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 966 998 2829
Size: 966 32 1831
Fragments arranged by size:
1831 966 32
Tth111I GACn'n_nGTC
Cuts at: 0 597 2829
Size: 597 2232
XbaI T'CTAG_A
Cuts at: 0 2583 2829
Size: 2583 246
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2412 2617 2829
Size: 2412 205 212
Fragments arranged by size:
2412 212 205
XmaI C'CCGG_G
Cuts at: 0 75 148 214 837 2829
Size: 75 73 66 623 1992
Fragments arranged by size:
1992 623 75 73 66
XmnI GAAnn'nnTTC
Cuts at: 0 1401 2829
Size: 1401 1428
ZraI GAC'GTC
Cuts at: 0 2730 2829
Size: 2730 99
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AleI
AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PasI PciI PflMI PfoI PpuMI PspOMI PstI PvuII
SacI SalI SanDI SapI SfcI SfoI SmaI SmlI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AflII AgeI AhdI AloI AscI AseI AsiSI
AvrII BaeI BcgI BfrBI BmgBI BsaAI BsiWI BsrGI
BssHII BstZ17I ClaI CspCI DraI DraIII FalI FseI
FspI FspAI HindIII HpaI MfeI NdeI NotI NruI
NsiI PacI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XhoI