Restriction map for
pFN21AB8502
(Linear) (Six-base) MAPSORT of: KIBB8502.seq Check: 3461 from: 1 to: 2592
KIBB8502.orf
With 182 enzymes: SgfI *
January 29, 2014 11:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 802 1249 2592
Size: 802 447 1343
Fragments arranged by size:
1343 802 447
AatII G_ACGT'C
Cuts at: 0 835 2592
Size: 835 1757
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 992 2592
Size: 992 1600
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1267 2592
Size: 1267 1325
AlwNI CAG_nnn'CTG
Cuts at: 0 21 357 1015 1089 1148 1368 2251 2466
Size: 21 336 658 74 59 220 883 215
Cuts at: 2466 2592
Size: 126
Fragments arranged by size:
883 658 336 220 215 126 74 59
21
ApaI G_GGCC'C
Cuts at: 0 242 346 2390 2592
Size: 242 104 2044 202
Fragments arranged by size:
2044 242 202 104
ApoI r'AATT_y
Cuts at: 0 177 2361 2592
Size: 177 2184 231
Fragments arranged by size:
2184 231 177
AvaI C'yCGr_G
Cuts at: 0 63 122 454 538 548 594 754 784
Size: 63 59 332 84 10 46 160 30
Cuts at: 784 2592
Size: 1808
Fragments arranged by size:
1808 332 160 84 63 59 46 30
10
AvrII C'CTAG_G
Cuts at: 0 2233 2592
Size: 2233 359
BanI G'GyrC_C
Cuts at: 0 43 187 391 637 780 1130 1555 2592
Size: 43 144 204 246 143 350 425 1037
Fragments arranged by size:
1037 425 350 246 204 144 143 43
BanII G_rGCy'C
Cuts at: 0 242 346 843 1076 1184 1393 1545 1736
Size: 242 104 497 233 108 209 152 191
Cuts at: 1736 2297 2390 2592
Size: 561 93 202
Fragments arranged by size:
561 497 242 233 209 202 191 152
108 104 93
BbeI G_GCGC'C
Cuts at: 0 47 395 641 784 2592
Size: 47 348 246 143 1808
Fragments arranged by size:
1808 348 246 143 47
BbsI GAAGACnn'nnnn_
Cuts at: 0 417 2592
Size: 417 2175
BciVI GTATCCnnnnn_n'
Cuts at: 0 1059 2592
Size: 1059 1533
BglI GCCn_nnn'nGGC
Cuts at: 0 1211 2507 2592
Size: 1211 1296 85
Fragments arranged by size:
1296 1211 85
BglII A'GATC_T
Cuts at: 0 2144 2428 2592
Size: 2144 284 164
Fragments arranged by size:
2144 284 164
BlpI GC'TnA_GC
Cuts at: 0 1405 1735 1951 2592
Size: 1405 330 216 641
Fragments arranged by size:
1405 641 330 216
Bme1580I G_kGCm'C
Cuts at: 0 190 242 346 1207 2390 2592
Size: 190 52 104 861 1183 202
Fragments arranged by size:
1183 861 202 190 104 52
BmrI ACTGGGnnnn_n'
Cuts at: 0 1473 2050 2164 2265 2592
Size: 1473 577 114 101 327
Fragments arranged by size:
1473 577 327 114 101
BmtI G_CTAG'C
Cuts at: 0 1225 2592
Size: 1225 1367
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 183 789 2166 2592
Size: 183 606 1377 426
Fragments arranged by size:
1377 606 426 183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 784 1168 1590 1802 1921 2107 2592
Size: 784 384 422 212 119 186 485
Fragments arranged by size:
784 485 422 384 212 186 119
Bpu10I CC'TnA_GC
Cuts at: 0 1394 2291 2592
Size: 1394 897 301
Fragments arranged by size:
1394 897 301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1420 2515 2592
Size: 1420 1095 77
Fragments arranged by size:
1420 1095 77
BsaI GGTCTCn'nnnn_
Cuts at: 0 457 2592
Size: 457 2135
BsaHI Gr'CG_yC
Cuts at: 0 44 392 422 497 638 781 832 2592
Size: 44 348 30 75 141 143 51 1760
Fragments arranged by size:
1760 348 143 141 75 51 44 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 451 481 1298 1328 2592
Size: 451 30 817 30 1264
Fragments arranged by size:
1264 817 451 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2228 2413 2592
Size: 2228 185 179
Fragments arranged by size:
2228 185 179
BseYI C'CCAG_C
Cuts at: 0 8 115 608 923 1063 1518 1966 2382
Size: 8 107 493 315 140 455 448 416
Cuts at: 2382 2418 2489 2592
Size: 36 71 103
Fragments arranged by size:
493 455 448 416 315 140 107 103
71 36 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 948 1237 2322 2592
Size: 343 605 289 1085 270
Fragments arranged by size:
1085 605 343 289 270
BsiEI CG_ry'CG
Cuts at: 0 231 695 708 861 935 2592
Size: 231 464 13 153 74 1657
Fragments arranged by size:
1657 464 231 153 74 13
BsiHKAI G_wGCw'C
Cuts at: 0 566 1076 1184 2297 2592
Size: 566 510 108 1113 295
Fragments arranged by size:
1113 566 510 295 108
BsiWI C'GTAC_G
Cuts at: 0 266 2592
Size: 266 2326
BsmI GAATG_Cn'
Cuts at: 0 1201 2592
Size: 1201 1391
BsmBI CGTCTCn'nnnn_
Cuts at: 0 435 2592
Size: 435 2157
Bsp1286I G_dGCh'C
Cuts at: 0 190 242 346 566 843 1076 1184 1207
Size: 190 52 104 220 277 233 108 23
Cuts at: 1207 1393 1545 1736 2297 2390 2592
Size: 186 152 191 561 93 202
Fragments arranged by size:
561 277 233 220 202 191 190 186
152 108 104 93 52 23
BspHI T'CATG_A
Cuts at: 0 2264 2592
Size: 2264 328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 223 802 1249 1259 1608 1865 2592
Size: 223 579 447 10 349 257 727
Fragments arranged by size:
727 579 447 349 257 223 10
BsrBI CCG'CTC
Cuts at: 0 773 791 863 2592
Size: 773 18 72 1729
Fragments arranged by size:
1729 773 72 18
BsrFI r'CCGG_y
Cuts at: 0 230 856 934 2592
Size: 230 626 78 1658
Fragments arranged by size:
1658 626 230 78
BsrGI T'GTAC_A
Cuts at: 0 467 2592
Size: 467 2125
BssHII G'CGCG_C
Cuts at: 0 660 2592
Size: 660 1932
BssSI C'ACGA_G
Cuts at: 0 2003 2284 2592
Size: 2003 281 308
Fragments arranged by size:
2003 308 281
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1148 1368 2592
Size: 1148 220 1224
Fragments arranged by size:
1224 1148 220
BstBI TT'CG_AA
Cuts at: 0 1416 2592
Size: 1416 1176
BstEII G'GTnAC_C
Cuts at: 0 288 2592
Size: 288 2304
BstXI CCAn_nnnn'nTGG
Cuts at: 0 285 1974 2063 2319 2592
Size: 285 1689 89 256 273
Fragments arranged by size:
1689 285 273 256 89
BstYI r'GATC_y
Cuts at: 0 1121 2144 2428 2592
Size: 1121 1023 284 164
Fragments arranged by size:
1121 1023 284 164
Bsu36I CC'TnA_GG
Cuts at: 0 718 1655 1741 1804 2592
Size: 718 937 86 63 788
Fragments arranged by size:
937 788 718 86 63
BtgI C'CryG_G
Cuts at: 0 38 293 377 724 929 1107 1551 2592
Size: 38 255 84 347 205 178 444 1041
Fragments arranged by size:
1041 444 347 255 205 178 84 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 586 2592
Size: 586 2006
BtsI GCAGTG_nn'
Cuts at: 0 865 965 1146 2592
Size: 865 100 181 1446
Fragments arranged by size:
1446 865 181 100
DraIII CAC_nnn'GTG
Cuts at: 0 256 1501 2592
Size: 256 1245 1091
Fragments arranged by size:
1245 1091 256
DrdI GACnn_nn'nnGTC
Cuts at: 0 133 2592
Size: 133 2459
EaeI y'GGCC_r
Cuts at: 0 228 348 692 858 888 932 1816 1976
Size: 228 120 344 166 30 44 884 160
Cuts at: 1976 2592
Size: 616
Fragments arranged by size:
884 616 344 228 166 160 120 44
30
EagI C'GGCC_G
Cuts at: 0 228 692 858 932 2592
Size: 228 464 166 74 1660
Fragments arranged by size:
1660 464 228 166 74
EarI CTCTTCn'nnn_
Cuts at: 0 554 1081 1789 2592
Size: 554 527 708 803
Fragments arranged by size:
803 708 554 527
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 895 989 2287 2592
Size: 895 94 1298 305
Fragments arranged by size:
1298 895 305 94
EcoICRI GAG'CTC
Cuts at: 0 1074 1182 2295 2592
Size: 1074 108 1113 297
Fragments arranged by size:
1113 1074 297 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 784 992 1168 1590 1802 1921 2107 2592
Size: 784 208 176 422 212 119 186 485
Fragments arranged by size:
784 485 422 212 208 186 176 119
EcoNI CCTnn'n_nnAGG
Cuts at: 0 717 1678 2592
Size: 717 961 914
Fragments arranged by size:
961 914 717
EcoO109I rG'GnC_Cy
Cuts at: 0 68 126 343 591 959 1267 1332 1365
Size: 68 58 217 248 368 308 65 33
Cuts at: 1365 1683 1854 2248 2346 2386 2550 2592
Size: 318 171 394 98 40 164 42
Fragments arranged by size:
394 368 318 308 248 217 171 164
98 68 65 58 42 40 33
EcoRI G'AATT_C
Cuts at: 0 177 2361 2592
Size: 177 2184 231
Fragments arranged by size:
2184 231 177
EcoRV GAT'ATC
Cuts at: 0 2010 2592
Size: 2010 582
FseI GG_CCGG'CC
Cuts at: 0 860 2592
Size: 860 1732
HaeII r_GCGC'y
Cuts at: 0 47 143 395 609 641 784 2592
Size: 47 96 252 214 32 143 1808
Fragments arranged by size:
1808 252 214 143 96 47 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1033 1065 1797 1829 2104 2136 2214 2246
Size: 1033 32 732 32 275 32 78 32
Cuts at: 2246 2592
Size: 346
Fragments arranged by size:
1033 732 346 275 78 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 1411 2592
Size: 1411 1181
KasI G'GCGC_C
Cuts at: 0 43 391 637 780 2592
Size: 43 348 246 143 1812
Fragments arranged by size:
1812 348 246 143 43
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2190 2580 2592
Size: 2190 390 12
Fragments arranged by size:
2190 390 12
MscI TGG'CCA
Cuts at: 0 350 1978 2592
Size: 350 1628 614
Fragments arranged by size:
1628 614 350
MslI CAynn'nnrTG
Cuts at: 0 475 1243 2592
Size: 475 768 1349
Fragments arranged by size:
1349 768 475
MspA1I CmG'CkG
Cuts at: 0 18 40 105 174 227 312 327 379
Size: 18 22 65 69 53 85 15 52
Cuts at: 379 726 931 1188 2592
Size: 347 205 257 1404
Fragments arranged by size:
1404 347 257 205 85 69 65 53
52 22 18 15
NaeI GCC'GGC
Cuts at: 0 232 858 936 2592
Size: 232 626 78 1656
Fragments arranged by size:
1656 626 232 78
NarI GG'CG_CC
Cuts at: 0 44 392 638 781 2592
Size: 44 348 246 143 1811
Fragments arranged by size:
1811 348 246 143 44
NcoI C'CATG_G
Cuts at: 0 1551 2592
Size: 1551 1041
NgoMIV G'CCGG_C
Cuts at: 0 230 856 934 2592
Size: 230 626 78 1658
Fragments arranged by size:
1658 626 230 78
NheI G'CTAG_C
Cuts at: 0 1221 2592
Size: 1221 1371
NspI r_CATG'y
Cuts at: 0 2166 2592
Size: 2166 426
PasI CC'CwG_GG
Cuts at: 0 1545 1884 2245 2592
Size: 1545 339 361 347
Fragments arranged by size:
1545 361 347 339
PflMI CCAn_nnn'nTGG
Cuts at: 0 1899 2592
Size: 1899 693
PfoI T'CCnGG_A
Cuts at: 0 504 2084 2592
Size: 504 1580 508
Fragments arranged by size:
1580 508 504
PpuMI rG'GwC_Cy
Cuts at: 0 68 126 591 1332 1854 2346 2592
Size: 68 58 465 741 522 492 246
Fragments arranged by size:
741 522 492 465 246 68 58
PshAI GACnn'nnGTC
Cuts at: 0 500 1607 2592
Size: 500 1107 985
Fragments arranged by size:
1107 985 500
PspOMI G'GGCC_C
Cuts at: 0 238 342 2386 2592
Size: 238 104 2044 206
Fragments arranged by size:
2044 238 206 104
PstI C_TGCA'G
Cuts at: 0 872 1085 1144 1373 1619 2333 2592
Size: 872 213 59 229 246 714 259
Fragments arranged by size:
872 714 259 246 229 213 59
PvuII CAG'CTG
Cuts at: 0 18 312 327 1188 2592
Size: 18 294 15 861 1404
Fragments arranged by size:
1404 861 294 18 15
SacI G_AGCT'C
Cuts at: 0 1076 1184 2297 2592
Size: 1076 108 1113 295
Fragments arranged by size:
1113 1076 295 108
SacII CC_GC'GG
Cuts at: 0 41 380 727 932 2592
Size: 41 339 347 205 1660
Fragments arranged by size:
1660 347 339 205 41
SanDI GG'GwC_CC
Cuts at: 0 68 126 591 2592
Size: 68 58 465 2001
Fragments arranged by size:
2001 465 68 58
SapI GCTCTTCn'nnn_
Cuts at: 0 554 1081 2592
Size: 554 527 1511
Fragments arranged by size:
1511 554 527
SbfI CC_TGCA'GG
Cuts at: 0 1373 1619 2592
Size: 1373 246 973
Fragments arranged by size:
1373 973 246
ScaI AGT'ACT
Cuts at: 0 2335 2592
Size: 2335 257
SexAI A'CCwGG_T
Cuts at: 0 1497 2592
Size: 1497 1095
SfcI C'TryA_G
Cuts at: 0 868 990 1081 1140 1369 1615 2329 2592
Size: 868 122 91 59 229 246 714 263
Fragments arranged by size:
868 714 263 246 229 122 91 59
SfoI GGC'GCC
Cuts at: 0 45 393 639 782 2592
Size: 45 348 246 143 1810
Fragments arranged by size:
1810 348 246 143 45
SmaI CCC'GGG
Cuts at: 0 65 124 596 786 2592
Size: 65 59 472 190 1806
Fragments arranged by size:
1806 472 190 65 59
SmlI C'TyrA_G
Cuts at: 0 548 1399 2530 2592
Size: 548 851 1131 62
Fragments arranged by size:
1131 851 548 62
StuI AGG'CCT
Cuts at: 0 748 2080 2592
Size: 748 1332 512
Fragments arranged by size:
1332 748 512
StyI C'CwwG_G
Cuts at: 0 918 1551 2233 2592
Size: 918 633 682 359
Fragments arranged by size:
918 682 633 359
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 439 746 2592
Size: 439 307 1846
Fragments arranged by size:
1846 439 307
TatI w'GTAC_w
Cuts at: 0 467 2333 2592
Size: 467 1866 259
Fragments arranged by size:
1866 467 259
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1298 1330 2592
Size: 1298 32 1262
Fragments arranged by size:
1298 1262 32
Tth111I GACn'n_nGTC
Cuts at: 0 2203 2592
Size: 2203 389
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1465 1558 2592
Size: 1465 93 1034
Fragments arranged by size:
1465 1034 93
XhoI C'TCGA_G
Cuts at: 0 548 2592
Size: 548 2044
XmaI C'CCGG_G
Cuts at: 0 63 122 594 784 2592
Size: 63 59 472 190 1808
Fragments arranged by size:
1808 472 190 63 59
XmnI GAAnn'nnTTC
Cuts at: 0 1798 2592
Size: 1798 794
ZraI GAC'GTC
Cuts at: 0 833 2592
Size: 833 1759
Enzymes that do cut:
AarI AatII AcuI AlfI AlwNI ApaI ApoI AvaI
AvrII BanI BanII BbeI BbsI BciVI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FseI HaeII Hin4I HindIII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PasI
PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI
SacII SanDI SapI SbfI ScaI SexAI SfcI SfoI
SmaI SmlI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaLI AscI AseI AsiSI BaeI BamHI
BbvCI BcgI BclI BfrBI BmgBI BsaAI BsaBI BsaWI
BspEI BsrDI BstZ17I ClaI CspCI DraI FalI FspI
FspAI HincII HpaI KpnI MfeI MluI NdeI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI RsrII SalI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI XbaI