Restriction map for
pFN21AB8401
(Linear) (Six-base) MAPSORT of: KIBB8401.seq Check: 609 from: 1 to: 2859
KIBB8401.orf
With 182 enzymes: SgfI *
July 15, 2010 18:31 ..
Acc65I G'GTAC_C
Cuts at: 0 1555 2859
Size: 1555 1304
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 190 1099 1526 2859
Size: 137 53 909 427 1333
Fragments arranged by size:
1333 909 427 137 53
AfeI AGC'GCT
Cuts at: 0 1288 2859
Size: 1288 1571
AflII C'TTAA_G
Cuts at: 0 624 2811 2859
Size: 624 2187 48
Fragments arranged by size:
2187 624 48
AflIII A'CryG_T
Cuts at: 0 26 1922 2859
Size: 26 1896 937
Fragments arranged by size:
1896 937 26
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2045 2859
Size: 2045 814
AlwNI CAG_nnn'CTG
Cuts at: 0 960 1249 1252 2173 2761 2859
Size: 960 289 3 921 588 98
Fragments arranged by size:
960 921 588 289 98 3
ApaLI G'TGCA_C
Cuts at: 0 818 2859
Size: 818 2041
ApoI r'AATT_y
Cuts at: 0 340 492 1219 1734 1835 2515 2536 2783
Size: 340 152 727 515 101 680 21 247
Cuts at: 2783 2859
Size: 76
Fragments arranged by size:
727 680 515 340 247 152 101 76
21
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1383 1416 2859
Size: 1383 33 1443
Fragments arranged by size:
1443 1383 33
BamHI G'GATC_C
Cuts at: 0 1379 2859
Size: 1379 1480
BanI G'GyrC_C
Cuts at: 0 1555 2859
Size: 1555 1304
BanII G_rGCy'C
Cuts at: 0 729 2859
Size: 729 2130
BbsI GAAGACnn'nnnn_
Cuts at: 0 1415 1616 2859
Size: 1415 201 1243
Fragments arranged by size:
1415 1243 201
BbvCI CC'TCA_GC
Cuts at: 0 1748 2859
Size: 1748 1111
BclI T'GATC_A
Cuts at: 0 639 2859
Size: 639 2220
BfrBI ATG'CAT
Cuts at: 0 310 577 2859
Size: 310 267 2282
Fragments arranged by size:
2282 310 267
Bme1580I G_kGCm'C
Cuts at: 0 822 1578 2859
Size: 822 756 1281
Fragments arranged by size:
1281 822 756
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 2444 2621 2859
Size: 251 2193 177 238
Fragments arranged by size:
2193 251 238 177
Bpu10I CC'TnA_GC
Cuts at: 0 1579 1748 2859
Size: 1579 169 1111
Fragments arranged by size:
1579 1111 169
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1644 1834 2859
Size: 1644 190 1025
Fragments arranged by size:
1644 1025 190
BsaBI GATnn'nnATC
Cuts at: 0 181 605 1829 2859
Size: 181 424 1224 1030
Fragments arranged by size:
1224 1030 424 181
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2316 2346 2859
Size: 2316 30 513
Fragments arranged by size:
2316 513 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1382 2324 2859
Size: 1382 942 535
Fragments arranged by size:
1382 942 535
BseYI C'CCAG_C
Cuts at: 0 793 2859
Size: 793 2066
BsiHKAI G_wGCw'C
Cuts at: 0 729 822 991 2859
Size: 729 93 169 1868
Fragments arranged by size:
1868 729 169 93
BsmI GAATG_Cn'
Cuts at: 0 579 1161 1310 1906 2859
Size: 579 582 149 596 953
Fragments arranged by size:
953 596 582 579 149
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2321 2859
Size: 2321 538
Bsp1286I G_dGCh'C
Cuts at: 0 729 822 991 1578 2859
Size: 729 93 169 587 1281
Fragments arranged by size:
1281 729 587 169 93
BspHI T'CATG_A
Cuts at: 0 939 2337 2859
Size: 939 1398 522
Fragments arranged by size:
1398 939 522
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1430 2859
Size: 1430 1429
BsrDI GCAATG_nn'
Cuts at: 0 1156 1693 2093 2859
Size: 1156 537 400 766
Fragments arranged by size:
1156 766 537 400
BsrFI r'CCGG_y
Cuts at: 0 2770 2859
Size: 2770 89
BstAPI GCAn_nnn'nTGC
Cuts at: 0 826 1249 2859
Size: 826 423 1610
Fragments arranged by size:
1610 826 423
BstEII G'GTnAC_C
Cuts at: 0 2145 2859
Size: 2145 714
BstYI r'GATC_y
Cuts at: 0 321 352 957 1035 1379 1433 1454 2118
Size: 321 31 605 78 344 54 21 664
Cuts at: 2118 2169 2292 2859
Size: 51 123 567
Fragments arranged by size:
664 605 567 344 321 123 78 54
51 31 21
DraI TTT'AAA
Cuts at: 0 2585 2859
Size: 2585 274
EaeI y'GGCC_r
Cuts at: 0 470 2772 2859
Size: 470 2302 87
Fragments arranged by size:
2302 470 87
EarI CTCTTCn'nnn_
Cuts at: 0 209 365 1050 1055 1802 1940 2859
Size: 209 156 685 5 747 138 919
Fragments arranged by size:
919 747 685 209 156 138 5
EcoICRI GAG'CTC
Cuts at: 0 727 2859
Size: 727 2132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 190 251 1099 1526 2444 2621 2859
Size: 137 53 61 848 427 918 177 238
Fragments arranged by size:
918 848 427 238 177 137 61 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1081 2859
Size: 1081 1778
EcoO109I rG'GnC_Cy
Cuts at: 0 1442 2646 2859
Size: 1442 1204 213
Fragments arranged by size:
1442 1204 213
EcoRI G'AATT_C
Cuts at: 0 2515 2783 2859
Size: 2515 268 76
Fragments arranged by size:
2515 268 76
EcoRV GAT'ATC
Cuts at: 0 972 2859
Size: 972 1887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2731 2859
Size: 2731 128
HaeII r_GCGC'y
Cuts at: 0 1290 2859
Size: 1290 1569
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 91 123 1027 1059 1939 1971 2316 2348
Size: 91 32 904 32 880 32 345 32
Cuts at: 2348 2813 2845 2859
Size: 465 32 14
Fragments arranged by size:
904 880 465 345 91 32 32 32
32 32 14
HincII GTy'rAC
Cuts at: 0 2202 2565 2615 2859
Size: 2202 363 50 244
Fragments arranged by size:
2202 363 244 50
HindIII A'AGCT_T
Cuts at: 0 119 2186 2859
Size: 119 2067 673
Fragments arranged by size:
2067 673 119
HpaI GTT'AAC
Cuts at: 0 2565 2859
Size: 2565 294
KpnI G_GTAC'C
Cuts at: 0 1559 2859
Size: 1559 1300
MfeI C'AATT_G
Cuts at: 0 1460 2152 2859
Size: 1460 692 707
Fragments arranged by size:
1460 707 692
MscI TGG'CCA
Cuts at: 0 472 2859
Size: 472 2387
MslI CAynn'nnrTG
Cuts at: 0 335 446 2572 2859
Size: 335 111 2126 287
Fragments arranged by size:
2126 335 287 111
MspA1I CmG'CkG
Cuts at: 0 672 1249 2070 2859
Size: 672 577 821 789
Fragments arranged by size:
821 789 672 577
NsiI A_TGCA'T
Cuts at: 0 312 579 2859
Size: 312 267 2280
Fragments arranged by size:
2280 312 267
NspI r_CATG'y
Cuts at: 0 428 1926 2859
Size: 428 1498 933
Fragments arranged by size:
1498 933 428
PacI TTA_AT'TAA
Cuts at: 0 35 2859
Size: 35 2824
PciI A'CATG_T
Cuts at: 0 1922 2859
Size: 1922 937
PflMI CCAn_nnn'nTGG
Cuts at: 0 2805 2859
Size: 2805 54
PfoI T'CCnGG_A
Cuts at: 0 165 2219 2859
Size: 165 2054 640
Fragments arranged by size:
2054 640 165
PpuMI rG'GwC_Cy
Cuts at: 0 1442 2859
Size: 1442 1417
PsiI TTA'TAA
Cuts at: 0 695 2859
Size: 695 2164
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 7 39 491 523 935 967 1514 1546
Size: 7 32 452 32 412 32 547 32
Cuts at: 1546 1697 1729 2859
Size: 151 32 1130
Fragments arranged by size:
1130 547 452 412 151 32 32 32
32 32 7
PstI C_TGCA'G
Cuts at: 0 671 1025 2859
Size: 671 354 1834
Fragments arranged by size:
1834 671 354
PvuII CAG'CTG
Cuts at: 0 672 1249 2070 2859
Size: 672 577 821 789
Fragments arranged by size:
821 789 672 577
SacI G_AGCT'C
Cuts at: 0 729 2859
Size: 729 2130
SapI GCTCTTCn'nnn_
Cuts at: 0 1055 2859
Size: 1055 1804
ScaI AGT'ACT
Cuts at: 0 630 950 2859
Size: 630 320 1909
Fragments arranged by size:
1909 630 320
SfcI C'TryA_G
Cuts at: 0 486 667 952 1021 1395 1520 2859
Size: 486 181 285 69 374 125 1339
Fragments arranged by size:
1339 486 374 285 181 125 69
SmlI C'TyrA_G
Cuts at: 0 624 1623 1849 2811 2859
Size: 624 999 226 962 48
Fragments arranged by size:
999 962 624 226 48
SpeI A'CTAG_T
Cuts at: 0 2851 2859
Size: 2851 8
SspI AAT'ATT
Cuts at: 0 2352 2859
Size: 2352 507
StyI C'CwwG_G
Cuts at: 0 1403 1446 2859
Size: 1403 43 1413
Fragments arranged by size:
1413 1403 43
SwaI ATTT'AAAT
Cuts at: 0 2585 2859
Size: 2585 274
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2164 2859
Size: 2164 695
TatI w'GTAC_w
Cuts at: 0 628 948 1992 2226 2859
Size: 628 320 1044 234 633
Fragments arranged by size:
1044 633 628 320 234
XbaI T'CTAG_A
Cuts at: 0 87 1048 1588 2859
Size: 87 961 540 1271
Fragments arranged by size:
1271 961 540 87
XmnI GAAnn'nnTTC
Cuts at: 0 161 251 344 1280 1334 1412 2859
Size: 161 90 93 936 54 78 1447
Fragments arranged by size:
1447 936 161 93 90 78 54
Enzymes that do cut:
Acc65I AcuI AfeI AflII AflIII AlfI AlwNI ApaLI
ApoI BaeI BamHI BanI BanII BbsI BbvCI BclI
BfrBI Bme1580I BpmI Bpu10I BpuEI BsaBI BsaXI BseRI
BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrDI
BsrFI BstAPI BstEII BstYI DraI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MscI MslI MspA1I
NsiI NspI PacI PciI PflMI PfoI PpuMI PsiI
PsrI PstI PvuII SacI SapI ScaI SfcI SmlI
SpeI SspI StyI SwaI TaqII TatI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AgeI AhdI AleI AloI
ApaI AscI AseI AsiSI AvaI AvrII BbeI BcgI
BciVI BglI BglII BlpI BmgBI BmrI BmtI BplI
BsaI BsaAI BsaHI BsaWI BsgI BsiEI BsiWI BspEI
BsrBI BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EagI
EciI FseI FspI FspAI KasI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PasI
PmeI PmlI PpiI PshAI PspOMI PspXI PvuI RsrII
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI StuI TstI Tth111I
XcmI XhoI XmaI ZraI