Restriction map for
pFN21AB8375
(Linear) (Six-base) MAPSORT of: KIBB8375.seq Check: 5830 from: 1 to: 1977
KIBB8375.orf
With 182 enzymes: SgfI *
July 15, 2010 18:31 ..
AatII G_ACGT'C
Cuts at: 0 686 1977
Size: 686 1291
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 1051 1732 1977
Size: 470 581 681 245
Fragments arranged by size:
681 581 470 245
AfeI AGC'GCT
Cuts at: 0 922 951 1248 1648 1977
Size: 922 29 297 400 329
Fragments arranged by size:
922 400 329 297 29
AflIII A'CryG_T
Cuts at: 0 253 1977
Size: 253 1724
AlwNI CAG_nnn'CTG
Cuts at: 0 1029 1977
Size: 1029 948
ApaI G_GGCC'C
Cuts at: 0 128 1977
Size: 128 1849
ApoI r'AATT_y
Cuts at: 0 235 1977
Size: 235 1742
AvaI C'yCGr_G
Cuts at: 0 44 560 1144 1977
Size: 44 516 584 833
Fragments arranged by size:
833 584 516 44
BamHI G'GATC_C
Cuts at: 0 1280 1977
Size: 1280 697
BanI G'GyrC_C
Cuts at: 0 118 519 930 1834 1977
Size: 118 401 411 904 143
Fragments arranged by size:
904 411 401 143 118
BanII G_rGCy'C
Cuts at: 0 63 128 530 1202 1316 1580 1977
Size: 63 65 402 672 114 264 397
Fragments arranged by size:
672 402 397 264 114 65 63
BbsI GAAGACnn'nnnn_
Cuts at: 0 975 1465 1977
Size: 975 490 512
Fragments arranged by size:
975 512 490
BbvCI CC'TCA_GC
Cuts at: 0 1011 1977
Size: 1011 966
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 163 197 1977
Size: 163 34 1780
Fragments arranged by size:
1780 163 34
BclI T'GATC_A
Cuts at: 0 263 1977
Size: 263 1714
BglI GCCn_nnn'nGGC
Cuts at: 0 1789 1977
Size: 1789 188
BglII A'GATC_T
Cuts at: 0 443 532 1977
Size: 443 89 1445
Fragments arranged by size:
1445 443 89
BlpI GC'TnA_GC
Cuts at: 0 22 940 1351 1977
Size: 22 918 411 626
Fragments arranged by size:
918 626 411 22
Bme1580I G_kGCm'C
Cuts at: 0 128 1695 1837 1977
Size: 128 1567 142 140
Fragments arranged by size:
1567 142 140 128
BmgBI CAC'GTC
Cuts at: 0 603 815 1323 1977
Size: 603 212 508 654
Fragments arranged by size:
654 603 508 212
BmrI ACTGGGnnnn_n'
Cuts at: 0 194 1683 1977
Size: 194 1489 294
Fragments arranged by size:
1489 294 194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 1255 1977
Size: 68 1187 722
Fragments arranged by size:
1187 722 68
Bpu10I CC'TnA_GC
Cuts at: 0 1011 1294 1977
Size: 1011 283 683
Fragments arranged by size:
1011 683 283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 829 1627 1786 1977
Size: 829 798 159 191
Fragments arranged by size:
829 798 191 159
BsaI GGTCTCn'nnnn_
Cuts at: 0 407 1431 1915 1977
Size: 407 1024 484 62
Fragments arranged by size:
1024 484 407 62
BsaAI yAC'GTr
Cuts at: 0 148 963 1977
Size: 148 815 1014
Fragments arranged by size:
1014 815 148
BsaHI Gr'CG_yC
Cuts at: 0 683 1977
Size: 683 1294
BsaWI w'CCGG_w
Cuts at: 0 1518 1977
Size: 1518 459
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 538 568 1367 1397 1609 1639 1977
Size: 538 30 799 30 212 30 338
Fragments arranged by size:
799 538 338 212 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 382 1324 1396 1744 1977
Size: 382 942 72 348 233
Fragments arranged by size:
942 382 348 233 72
BseYI C'CCAG_C
Cuts at: 0 711 1096 1502 1551 1621 1784 1960 1977
Size: 711 385 406 49 70 163 176 17
Fragments arranged by size:
711 406 385 176 163 70 49 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 757 759 871 1977
Size: 250 507 2 112 1106
Fragments arranged by size:
1106 507 250 112 2
BsiEI CG_ry'CG
Cuts at: 0 434 1104 1260 1399 1715 1977
Size: 434 670 156 139 316 262
Fragments arranged by size:
670 434 316 262 156 139
BsiHKAI G_wGCw'C
Cuts at: 0 53 1316 1977
Size: 53 1263 661
Fragments arranged by size:
1263 661 53
BsmI GAATG_Cn'
Cuts at: 0 281 1562 1977
Size: 281 1281 415
Fragments arranged by size:
1281 415 281
BsmBI CGTCTCn'nnnn_
Cuts at: 0 17 154 805 1078 1728 1977
Size: 17 137 651 273 650 249
Fragments arranged by size:
651 650 273 249 137 17
Bsp1286I G_dGCh'C
Cuts at: 0 53 63 128 530 1202 1316 1580 1695
Size: 53 10 65 402 672 114 264 115
Cuts at: 1695 1837 1977
Size: 142 140
Fragments arranged by size:
672 402 264 142 140 115 114 65
53 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 781 1977
Size: 781 1196
BsrBI CCG'CTC
Cuts at: 0 38 1278 1977
Size: 38 1240 699
Fragments arranged by size:
1240 699 38
BsrFI r'CCGG_y
Cuts at: 0 988 1977
Size: 988 989
BssSI C'ACGA_G
Cuts at: 0 862 1688 1977
Size: 862 826 289
Fragments arranged by size:
862 826 289
BstAPI GCAn_nnn'nTGC
Cuts at: 0 550 1977
Size: 550 1427
BstXI CCAn_nnnn'nTGG
Cuts at: 0 723 1180 1977
Size: 723 457 797
Fragments arranged by size:
797 723 457
BstYI r'GATC_y
Cuts at: 0 443 532 570 1280 1977
Size: 443 89 38 710 697
Fragments arranged by size:
710 697 443 89 38
Bsu36I CC'TnA_GG
Cuts at: 0 408 1977
Size: 408 1569
BtgI C'CryG_G
Cuts at: 0 434 836 1977
Size: 434 402 1141
Fragments arranged by size:
1141 434 402
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 121 1977
Size: 121 1856
BtsI GCAGTG_nn'
Cuts at: 0 1365 1977
Size: 1365 612
ClaI AT'CG_AT
Cuts at: 0 689 1977
Size: 689 1288
DrdI GACnn_nn'nnGTC
Cuts at: 0 1423 1977
Size: 1423 554
EaeI y'GGCC_r
Cuts at: 0 431 990 1101 1257 1696 1712 1977
Size: 431 559 111 156 439 16 265
Fragments arranged by size:
559 439 431 265 156 111 16
EagI C'GGCC_G
Cuts at: 0 431 1101 1257 1712 1977
Size: 431 670 156 455 265
Fragments arranged by size:
670 455 431 265 156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 30 1977
Size: 30 1947
EcoICRI GAG'CTC
Cuts at: 0 1314 1977
Size: 1314 663
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 470 1051 1255 1732 1977
Size: 68 402 581 204 477 245
Fragments arranged by size:
581 477 402 245 204 68
EcoO109I rG'GnC_Cy
Cuts at: 0 125 246 1055 1290 1678 1977
Size: 125 121 809 235 388 299
Fragments arranged by size:
809 388 299 235 125 121
EcoRI G'AATT_C
Cuts at: 0 235 1977
Size: 235 1742
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 47 1977
Size: 47 1930
HaeII r_GCGC'y
Cuts at: 0 924 953 1030 1250 1650 1977
Size: 924 29 77 220 400 327
Fragments arranged by size:
924 400 327 220 77 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 64 96 1522 1554 1977
Size: 64 32 1426 32 423
Fragments arranged by size:
1426 423 64 32 32
HincII GTy'rAC
Cuts at: 0 1843 1977
Size: 1843 134
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 650 1952 1977
Size: 650 1302 25
Fragments arranged by size:
1302 650 25
MslI CAynn'nnrTG
Cuts at: 0 781 787 1977
Size: 781 6 1190
Fragments arranged by size:
1190 781 6
MspA1I CmG'CkG
Cuts at: 0 430 733 806 936 1100 1350 1788 1960
Size: 430 303 73 130 164 250 438 172
Cuts at: 1960 1977
Size: 17
Fragments arranged by size:
438 430 303 250 172 164 130 73
17
NcoI C'CATG_G
Cuts at: 0 836 1977
Size: 836 1141
NspI r_CATG'y
Cuts at: 0 257 466 1977
Size: 257 209 1511
Fragments arranged by size:
1511 257 209
PasI CC'CwG_GG
Cuts at: 0 1886 1977
Size: 1886 91
PciI A'CATG_T
Cuts at: 0 253 1977
Size: 253 1724
PflMI CCAn_nnn'nTGG
Cuts at: 0 867 1044 1212 1977
Size: 867 177 168 765
Fragments arranged by size:
867 765 177 168
PfoI T'CCnGG_A
Cuts at: 0 155 318 722 1232 1316 1604 1977
Size: 155 163 404 510 84 288 373
Fragments arranged by size:
510 404 373 288 163 155 84
PmlI CAC'GTG
Cuts at: 0 148 1977
Size: 148 1829
PpuMI rG'GwC_Cy
Cuts at: 0 246 1290 1678 1977
Size: 246 1044 388 299
Fragments arranged by size:
1044 388 299 246
PshAI GACnn'nnGTC
Cuts at: 0 166 1977
Size: 166 1811
PsiI TTA'TAA
Cuts at: 0 221 1977
Size: 221 1756
PspOMI G'GGCC_C
Cuts at: 0 124 1977
Size: 124 1853
PstI C_TGCA'G
Cuts at: 0 1122 1391 1589 1977
Size: 1122 269 198 388
Fragments arranged by size:
1122 388 269 198
PvuII CAG'CTG
Cuts at: 0 733 1350 1977
Size: 733 617 627
Fragments arranged by size:
733 627 617
SacI G_AGCT'C
Cuts at: 0 1316 1977
Size: 1316 661
SanDI GG'GwC_CC
Cuts at: 0 1678 1977
Size: 1678 299
SexAI A'CCwGG_T
Cuts at: 0 611 1977
Size: 611 1366
SfcI C'TryA_G
Cuts at: 0 819 1118 1387 1585 1977
Size: 819 299 269 198 392
Fragments arranged by size:
819 392 299 269 198
SmaI CCC'GGG
Cuts at: 0 46 562 1977
Size: 46 516 1415
Fragments arranged by size:
1415 516 46
SmlI C'TyrA_G
Cuts at: 0 844 1642 1801 1977
Size: 844 798 159 176
Fragments arranged by size:
844 798 176 159
StuI AGG'CCT
Cuts at: 0 458 476 491 1009 1977
Size: 458 18 15 518 968
Fragments arranged by size:
968 518 458 18 15
StyI C'CwwG_G
Cuts at: 0 836 998 1977
Size: 836 162 979
Fragments arranged by size:
979 836 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1899 1977
Size: 1899 78
TatI w'GTAC_w
Cuts at: 0 1331 1977
Size: 1331 646
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 137 169 1367 1399 1977
Size: 137 32 1198 32 578
Fragments arranged by size:
1198 578 137 32 32
XbaI T'CTAG_A
Cuts at: 0 55 1977
Size: 55 1922
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 564 1209 1939 1977
Size: 564 645 730 38
Fragments arranged by size:
730 645 564 38
XmaI C'CCGG_G
Cuts at: 0 44 560 1977
Size: 44 516 1417
Fragments arranged by size:
1417 516 44
ZraI GAC'GTC
Cuts at: 0 684 1977
Size: 684 1293
Enzymes that do cut:
AatII AcuI AfeI AflIII AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbsI BbvCI BcgI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DrdI EaeI EagI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI HaeII Hin4I HincII MmeI MslI MspA1I
NcoI NspI PasI PciI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PstI PvuII SacI SanDI SexAI
SfcI SmaI SmlI StuI StyI TaqII TatI TstI
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BciVI BfrBI BmtI BplI BsaBI BsiWI BspEI
BspHI BsrDI BsrGI BssHII BstBI BstEII BstZ17I CspCI
DraI DraIII EarI EcoNI EcoRV FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PmeI PpiI PspXI PsrI PvuI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XhoI XmnI