(Linear) (Six-base) MAPSORT of: KIBB8371.seq  Check: 3758  from: 1  to: 1557



KIBB8371.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:28  ..

AccI GT'mk_AC

Cuts at:      0     959    1557 
   Size:        959     598
 
AflIII A'CryG_T

Cuts at:      0     977    1557 
   Size:        977     580
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     146     178    1557 
   Size:        146      32    1379

  Fragments arranged by size: 

                1379     146      32
 
ApaI G_GGCC'C

Cuts at:      0    1509    1557 
   Size:       1509      48
 
ApoI r'AATT_y

Cuts at:      0     237    1557 
   Size:        237    1320
 
AvaI C'yCGr_G

Cuts at:      0      20    1557 
   Size:         20    1537
 
BanII G_rGCy'C

Cuts at:      0    1509    1557 
   Size:       1509      48
 
BclI T'GATC_A

Cuts at:      0     531    1557 
   Size:        531    1026
 
Bme1580I G_kGCm'C

Cuts at:      0    1509    1557 
   Size:       1509      48
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     536    1040    1427    1557 
   Size:        536     504     387     130

  Fragments arranged by size: 

                 536     504     387     130
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     479    1071    1557 
   Size:        479     592     486

  Fragments arranged by size: 

                 592     486     479
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     146     176     545     575    1557 
   Size:        146      30     369      30     982

  Fragments arranged by size: 

                 982     369     146      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     847    1557 
   Size:        847     710
 
BseYI C'CCAG_C

Cuts at:      0     126     392    1243    1557 
   Size:        126     266     851     314

  Fragments arranged by size: 

                 851     314     266     126
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     961    1557 
   Size:        961     596
 
Bsp1286I G_dGCh'C

Cuts at:      0    1509    1557 
   Size:       1509      48
 
BspHI T'CATG_A

Cuts at:      0     900    1557 
   Size:        900     657
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     198    1557 
   Size:        198    1359
 
BsrDI GCAATG_nn'

Cuts at:      0     756    1557 
   Size:        756     801
 
BsrGI T'GTAC_A

Cuts at:      0     473     980    1557 
   Size:        473     507     577

  Fragments arranged by size: 

                 577     507     473
 
BssSI C'ACGA_G

Cuts at:      0     412    1557 
   Size:        412    1145
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1077    1336    1557 
   Size:       1077     259     221

  Fragments arranged by size: 

                1077     259     221
 
BstYI r'GATC_y

Cuts at:      0     216    1557 
   Size:        216    1341
 
BtsI GCAGTG_nn'

Cuts at:      0     290    1341    1557 
   Size:        290    1051     216

  Fragments arranged by size: 

                1051     290     216
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     536    1040    1427    1557 
   Size:        536     504     387     130

  Fragments arranged by size: 

                 536     504     387     130
 
EcoRI G'AATT_C

Cuts at:      0     237    1557 
   Size:        237    1320
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1173    1205    1557 
   Size:       1173      32     352

  Fragments arranged by size: 

                1173     352      32
 
HincII GTy'rAC

Cuts at:      0     873    1557 
   Size:        873     684
 
MslI CAynn'nnrTG

Cuts at:      0     905    1175    1273    1557 
   Size:        905     270      98     284

  Fragments arranged by size: 

                 905     284     270      98
 
NdeI CA'TA_TG

Cuts at:      0     203    1557 
   Size:        203    1354
 
NspI r_CATG'y

Cuts at:      0     981    1557 
   Size:        981     576
 
PciI A'CATG_T

Cuts at:      0     977    1557 
   Size:        977     580
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     146     178     503     535    1557 
   Size:        146      32     325      32    1022

  Fragments arranged by size: 

                1022     325     146      32      32
 
PshAI GACnn'nnGTC

Cuts at:      0     653    1557 
   Size:        653     904
 
PspOMI G'GGCC_C

Cuts at:      0    1505    1557 
   Size:       1505      52
 
PstI C_TGCA'G

Cuts at:      0    1138    1557 
   Size:       1138     419
 
SexAI A'CCwGG_T

Cuts at:      0      98    1557 
   Size:         98    1459
 
SfcI C'TryA_G

Cuts at:      0    1103    1134    1557 
   Size:       1103      31     423

  Fragments arranged by size: 

                1103     423      31
 
SmlI C'TyrA_G

Cuts at:      0     494    1086    1557 
   Size:        494     592     471

  Fragments arranged by size: 

                 592     494     471
 
SpeI A'CTAG_T

Cuts at:      0     252    1557 
   Size:        252    1305
 
StuI AGG'CCT

Cuts at:      0    1375    1557 
   Size:       1375     182
 
StyI C'CwwG_G

Cuts at:      0     456    1557 
   Size:        456    1101
 
TatI w'GTAC_w

Cuts at:      0     473     980    1513    1557 
   Size:        473     507     533      44

  Fragments arranged by size: 

                 533     507     473      44
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     162     194    1451    1483    1557 
   Size:        162      32    1257      32      74

  Fragments arranged by size: 

                1257     162      74      32      32
 

 Enzymes that do cut:

     AccI   AflIII     AloI     ApaI     ApoI     AvaI    BanII     BclI
 Bme1580I     BpmI    BpuEI    BsaXI    BseRI    BseYI    BsmBI Bsp1286I
    BspHI    BspMI    BsrDI    BsrGI    BssSI    BstXI    BstYI     BtsI
  Eco57MI    EcoRI    Hin4I   HincII     MslI     NdeI     NspI     PciI
     PpiI    PshAI   PspOMI     PstI    SexAI     SfcI     SmlI     SpeI
     StuI     StyI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI    BsrBI    BsrFI   BssHII
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI
    PpuMI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI     SspI     SwaI
    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI