(Linear) (Six-base) MAPSORT of: KIBB8343.seq  Check: 6890  from: 1  to: 684



KIBB8343.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:28  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448     684 
   Size:        448     236
 
AleI CACnn'nnGTG

Cuts at:      0     112     684 
   Size:        112     572
 
BanI G'GyrC_C

Cuts at:      0     106     378     684 
   Size:        106     272     306

  Fragments arranged by size: 

                 306     272     106
 
Bme1580I G_kGCm'C

Cuts at:      0     383     684 
   Size:        383     301
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     415     684 
   Size:        415     269
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     249     533     684 
   Size:        249     284     151

  Fragments arranged by size: 

                 284     249     151
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     279     684 
   Size:        279     405
 
BsiHKAI G_wGCw'C

Cuts at:      0      59     684 
   Size:         59     625
 
Bsp1286I G_dGCh'C

Cuts at:      0      59     383     684 
   Size:         59     324     301

  Fragments arranged by size: 

                 324     301      59
 
BstYI r'GATC_y

Cuts at:      0     570     684 
   Size:        570     114
 
BtgI C'CryG_G

Cuts at:      0      80     353     684 
   Size:         80     273     331

  Fragments arranged by size: 

                 331     273      80
 
BtsI GCAGTG_nn'

Cuts at:      0     631     684 
   Size:        631      53
 
EarI CTCTTCn'nnn_

Cuts at:      0     143     424     440     452     460     684 
   Size:        143     281      16      12       8     224

  Fragments arranged by size: 

                 281     224     143      16      12       8
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     249     448     533     684 
   Size:        249     199      85     151

  Fragments arranged by size: 

                 249     199     151      85
 
EcoO109I rG'GnC_Cy

Cuts at:      0     174     684 
   Size:        174     510
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      46      78     606     638     684 
   Size:         46      32     528      32      46

  Fragments arranged by size: 

                 528      46      46      32      32
 
MslI CAynn'nnrTG

Cuts at:      0     112     684 
   Size:        112     572
 
NcoI C'CATG_G

Cuts at:      0      80     353     684 
   Size:         80     273     331

  Fragments arranged by size: 

                 331     273      80
 
NdeI CA'TA_TG

Cuts at:      0     499     684 
   Size:        499     185
 
NspI r_CATG'y

Cuts at:      0      20     131     684 
   Size:         20     111     553

  Fragments arranged by size: 

                 553     111      20
 
PasI CC'CwG_GG

Cuts at:      0     177     684 
   Size:        177     507
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     227     684 
   Size:        227     457
 
PfoI T'CCnGG_A

Cuts at:      0     226     684 
   Size:        226     458
 
PshAI GACnn'nnGTC

Cuts at:      0     479     684 
   Size:        479     205
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     511     543     684 
   Size:        511      32     141

  Fragments arranged by size: 

                 511     141      32
 
PstI C_TGCA'G

Cuts at:      0      92     684 
   Size:         92     592
 
SfcI C'TryA_G

Cuts at:      0      88     684 
   Size:         88     596
 
SmlI C'TyrA_G

Cuts at:      0     294     684 
   Size:        294     390
 
StyI C'CwwG_G

Cuts at:      0      80     353     655     684 
   Size:         80     273     302      29

  Fragments arranged by size: 

                 302     273      80      29
 
TatI w'GTAC_w

Cuts at:      0     402     449     684 
   Size:        402      47     235

  Fragments arranged by size: 

                 402     235      47
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     306     338     684 
   Size:        306      32     346

  Fragments arranged by size: 

                 346     306      32
 

 Enzymes that do cut:

     AcuI     AleI     BanI Bme1580I     BmrI     BpmI    BpuEI  BsiHKAI
 Bsp1286I    BstYI     BtgI     BtsI     EarI  Eco57MI EcoO109I    Hin4I
     MslI     NcoI     NdeI     NspI     PasI    PflMI     PfoI    PshAI
     PsrI     PstI     SfcI     SmlI     StyI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI
     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI