Restriction map for
pFN21AB8287
(Linear) (Six-base) MAPSORT of: KIBB8287.seq Check: 5930 from: 1 to: 1266
KIBB8287.orf
With 182 enzymes: SgfI *
July 15, 2010 18:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 194 238 1266
Size: 194 44 1028
Fragments arranged by size:
1028 194 44
AccI GT'mk_AC
Cuts at: 0 898 1266
Size: 898 368
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1038 1266
Size: 1038 228
AgeI A'CCGG_T
Cuts at: 0 893 1266
Size: 893 373
AlwNI CAG_nnn'CTG
Cuts at: 0 207 1266
Size: 207 1059
AvaI C'yCGr_G
Cuts at: 0 65 1266
Size: 65 1201
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1185 1218 1266
Size: 1185 33 48
Fragments arranged by size:
1185 48 33
BanII G_rGCy'C
Cuts at: 0 730 984 1266
Size: 730 254 282
Fragments arranged by size:
730 282 254
BfrBI ATG'CAT
Cuts at: 0 703 1266
Size: 703 563
BmrI ACTGGGnnnn_n'
Cuts at: 0 1106 1116 1190 1266
Size: 1106 10 74 76
Fragments arranged by size:
1106 76 74 10
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 749 1266
Size: 749 517
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 492 866 950 1034 1266
Size: 145 347 374 84 84 232
Fragments arranged by size:
374 347 232 145 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 805 1266
Size: 336 469 461
Fragments arranged by size:
469 461 336
BsaI GGTCTCn'nnnn_
Cuts at: 0 121 439 1266
Size: 121 318 827
Fragments arranged by size:
827 318 121
BsaAI yAC'GTr
Cuts at: 0 1151 1266
Size: 1151 115
BsaHI Gr'CG_yC
Cuts at: 0 578 1266
Size: 578 688
BsaWI w'CCGG_w
Cuts at: 0 423 893 1142 1266
Size: 423 470 249 124
Fragments arranged by size:
470 423 249 124
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 1266
Size: 85 1181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 487 1266
Size: 266 221 779
Fragments arranged by size:
779 266 221
BsiHKAI G_wGCw'C
Cuts at: 0 261 730 1150 1266
Size: 261 469 420 116
Fragments arranged by size:
469 420 261 116
BsmI GAATG_Cn'
Cuts at: 0 700 1120 1266
Size: 700 420 146
Fragments arranged by size:
700 420 146
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1 1266
Size: 1 1265
Bsp1286I G_dGCh'C
Cuts at: 0 261 730 984 1150 1266
Size: 261 469 254 166 116
Fragments arranged by size:
469 261 254 166 116
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 194 238 800 1220 1266
Size: 194 44 562 420 46
Fragments arranged by size:
562 420 194 46 44
BsrBI CCG'CTC
Cuts at: 0 309 1266
Size: 309 957
BsrDI GCAATG_nn'
Cuts at: 0 705 1266
Size: 705 561
BsrFI r'CCGG_y
Cuts at: 0 893 1266
Size: 893 373
BstEII G'GTnAC_C
Cuts at: 0 33 1266
Size: 33 1233
BtsI GCAGTG_nn'
Cuts at: 0 625 1266
Size: 625 641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 603 638 687 722 1266
Size: 603 35 49 35 544
Fragments arranged by size:
603 544 49 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 1124 1266
Size: 1124 142
EarI CTCTTCn'nnn_
Cuts at: 0 214 1266
Size: 214 1052
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1266
Size: 19 1247
EcoICRI GAG'CTC
Cuts at: 0 728 1266
Size: 728 538
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 492 866 950 1034 1038 1266
Size: 145 347 374 84 84 4 228
Fragments arranged by size:
374 347 228 145 84 84 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 439 1266
Size: 439 827
EcoO109I rG'GnC_Cy
Cuts at: 0 101 920 1266
Size: 101 819 346
Fragments arranged by size:
819 346 101
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 484 1266
Size: 484 782
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 514 546 1266
Size: 514 32 720
Fragments arranged by size:
720 514 32
MslI CAynn'nnrTG
Cuts at: 0 551 679 777 1266
Size: 551 128 98 489
Fragments arranged by size:
551 489 128 98
MspA1I CmG'CkG
Cuts at: 0 84 1086 1266
Size: 84 1002 180
Fragments arranged by size:
1002 180 84
NsiI A_TGCA'T
Cuts at: 0 705 1266
Size: 705 561
NspI r_CATG'y
Cuts at: 0 238 707 1043 1266
Size: 238 469 336 223
Fragments arranged by size:
469 336 238 223
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 248 280 1266
Size: 248 32 986
Fragments arranged by size:
986 248 32
PpuMI rG'GwC_Cy
Cuts at: 0 101 920 1266
Size: 101 819 346
Fragments arranged by size:
819 346 101
PstI C_TGCA'G
Cuts at: 0 1247 1266
Size: 1247 19
PvuII CAG'CTG
Cuts at: 0 84 1266
Size: 84 1182
SacI G_AGCT'C
Cuts at: 0 730 1266
Size: 730 536
SfcI C'TryA_G
Cuts at: 0 1243 1266
Size: 1243 23
SmaI CCC'GGG
Cuts at: 0 67 1266
Size: 67 1199
SmlI C'TyrA_G
Cuts at: 0 351 784 1266
Size: 351 433 482
Fragments arranged by size:
482 433 351
StuI AGG'CCT
Cuts at: 0 227 799 883 1051 1219 1266
Size: 227 572 84 168 168 47
Fragments arranged by size:
572 227 168 168 84 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 904 1135 1266
Size: 904 231 131
Fragments arranged by size:
904 231 131
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 174 1266
Size: 174 1092
XmaI C'CCGG_G
Cuts at: 0 65 1266
Size: 65 1201
XmnI GAAnn'nnTTC
Cuts at: 0 799 1219 1266
Size: 799 420 47
Fragments arranged by size:
799 420 47
Enzymes that do cut:
AarI AccI AcuI AgeI AlwNI AvaI BaeI BanII
BfrBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaHI
BsaWI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BstEII BtsI CspCI DraIII EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I MslI
MspA1I NsiI NspI PpiI PpuMI PstI PvuII SacI
SfcI SmaI SmlI StuI TaqII XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BamHI BanI BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmtI Bpu10I
BsaBI BsaXI BseYI BsiEI BsiWI BspEI BspHI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI ClaI DraI DrdI EaeI EagI EcoRI
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PflMI PfoI PmeI PmlI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StyI SwaI TatI TstI
Tth111I XbaI XhoI ZraI