Restriction map for
pFN21AB8259
(Linear) (Six-base) MAPSORT of: KIBB8259.seq Check: 5150 from: 1 to: 897
KIBB8259.orf
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 134 787 897
Size: 134 653 110
Fragments arranged by size:
653 134 110
AccI GT'mk_AC
Cuts at: 0 361 897
Size: 361 536
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 607 712 897
Size: 121 486 105 185
Fragments arranged by size:
486 185 121 105
AleI CACnn'nnGTG
Cuts at: 0 817 897
Size: 817 80
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 301 897
Size: 301 596
AvaI C'yCGr_G
Cuts at: 0 140 297 897
Size: 140 157 600
Fragments arranged by size:
600 157 140
BamHI G'GATC_C
Cuts at: 0 321 897
Size: 321 576
BanI G'GyrC_C
Cuts at: 0 354 390 897
Size: 354 36 507
Fragments arranged by size:
507 354 36
BanII G_rGCy'C
Cuts at: 0 181 845 897
Size: 181 664 52
Fragments arranged by size:
664 181 52
BfrBI ATG'CAT
Cuts at: 0 518 897
Size: 518 379
BglI GCCn_nnn'nGGC
Cuts at: 0 313 897
Size: 313 584
Bme1580I G_kGCm'C
Cuts at: 0 357 897
Size: 357 540
BmgBI CAC'GTC
Cuts at: 0 293 897
Size: 293 604
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 897
Size: 115 782
BsaAI yAC'GTr
Cuts at: 0 468 897
Size: 468 429
BsaWI w'CCGG_w
Cuts at: 0 387 897
Size: 387 510
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 369 399 897
Size: 369 30 498
Fragments arranged by size:
498 369 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 202 685 897
Size: 31 171 483 212
Fragments arranged by size:
483 212 171 31
BseYI C'CCAG_C
Cuts at: 0 325 644 897
Size: 325 319 253
Fragments arranged by size:
325 319 253
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 789 810 841 897
Size: 106 683 21 31 56
Fragments arranged by size:
683 106 56 31 21
BsiHKAI G_wGCw'C
Cuts at: 0 181 737 897
Size: 181 556 160
Fragments arranged by size:
556 181 160
BsmI GAATG_Cn'
Cuts at: 0 520 897
Size: 520 377
Bsp1286I G_dGCh'C
Cuts at: 0 181 357 737 845 897
Size: 181 176 380 108 52
Fragments arranged by size:
380 181 176 108 52
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 134 617 787 897
Size: 134 483 170 110
Fragments arranged by size:
483 170 134 110
BsrBI CCG'CTC
Cuts at: 0 267 897
Size: 267 630
BsrDI GCAATG_nn'
Cuts at: 0 194 897
Size: 194 703
BstAPI GCAn_nnn'nTGC
Cuts at: 0 422 897
Size: 422 475
BstEII G'GTnAC_C
Cuts at: 0 54 897
Size: 54 843
BstYI r'GATC_y
Cuts at: 0 321 698 897
Size: 321 377 199
Fragments arranged by size:
377 321 199
BtgI C'CryG_G
Cuts at: 0 80 897
Size: 80 817
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 840 855 875 890 897
Size: 840 15 20 15 7
Fragments arranged by size:
840 20 15 15 7
EaeI y'GGCC_r
Cuts at: 0 203 470 677 897
Size: 203 267 207 220
Fragments arranged by size:
267 220 207 203
EarI CTCTTCn'nnn_
Cuts at: 0 8 58 897
Size: 8 50 839
Fragments arranged by size:
839 50 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 630 897
Size: 630 267
EcoICRI GAG'CTC
Cuts at: 0 179 897
Size: 179 718
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 121 607 712 897
Size: 115 6 486 105 185
Fragments arranged by size:
486 185 115 105 6
EcoO109I rG'GnC_Cy
Cuts at: 0 315 897
Size: 315 582
FspI TGC'GCA
Cuts at: 0 415 897
Size: 415 482
HaeII r_GCGC'y
Cuts at: 0 495 897
Size: 495 402
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 481 513 897
Size: 481 32 384
Fragments arranged by size:
481 384 32
HincII GTy'rAC
Cuts at: 0 159 362 897
Size: 159 203 535
Fragments arranged by size:
535 203 159
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 513 605 897
Size: 513 92 292
Fragments arranged by size:
513 292 92
MscI TGG'CCA
Cuts at: 0 205 472 679 897
Size: 205 267 207 218
Fragments arranged by size:
267 218 207 205
MslI CAynn'nnrTG
Cuts at: 0 544 817 897
Size: 544 273 80
Fragments arranged by size:
544 273 80
MspA1I CmG'CkG
Cuts at: 0 442 884 897
Size: 442 442 13
Fragments arranged by size:
442 442 13
NcoI C'CATG_G
Cuts at: 0 80 897
Size: 80 817
NsiI A_TGCA'T
Cuts at: 0 520 897
Size: 520 377
NspI r_CATG'y
Cuts at: 0 282 897
Size: 282 615
PasI CC'CwG_GG
Cuts at: 0 350 897
Size: 350 547
PfoI T'CCnGG_A
Cuts at: 0 223 236 524 897
Size: 223 13 288 373
Fragments arranged by size:
373 288 223 13
PmlI CAC'GTG
Cuts at: 0 468 897
Size: 468 429
PspXI vC'TCGA_Gb
Cuts at: 0 297 897
Size: 297 600
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 793 825 897
Size: 793 32 72
Fragments arranged by size:
793 72 32
PstI C_TGCA'G
Cuts at: 0 452 897
Size: 452 445
SacI G_AGCT'C
Cuts at: 0 181 897
Size: 181 716
SalI G'TCGA_C
Cuts at: 0 360 897
Size: 360 537
SapI GCTCTTCn'nnn_
Cuts at: 0 58 897
Size: 58 839
SfcI C'TryA_G
Cuts at: 0 448 897
Size: 448 449
SmlI C'TyrA_G
Cuts at: 0 297 897
Size: 297 600
SphI G_CATG'C
Cuts at: 0 282 897
Size: 282 615
StyI C'CwwG_G
Cuts at: 0 80 716 897
Size: 80 636 181
Fragments arranged by size:
636 181 80
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 366 369 398 401 897
Size: 366 3 29 3 496
Fragments arranged by size:
496 366 29 3 3
XhoI C'TCGA_G
Cuts at: 0 297 897
Size: 297 600
Enzymes that do cut:
AarI AccI AcuI AleI AlfI AvaI BamHI BanI
BanII BfrBI BglI Bme1580I BmgBI BpmI BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrDI BstAPI BstEII BstYI BtgI CspCI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I
HincII MmeI MscI MslI MspA1I NcoI NsiI NspI
PasI PfoI PmlI PspXI PsrI PstI SacI SalI
SapI SfcI SmlI SphI StyI TstI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaBI BsaHI BsiEI BsiWI BsmBI BspEI BspHI BsrFI
BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgZI
BtsI ClaI DraI DraIII DrdI EagI EcoNI EcoRI
EcoRV FalI FseI FspAI HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PvuI PvuII RsrII SacII SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI TaqII TatI Tth111I
XbaI XcmI XmaI XmnI ZraI