(Linear) (Six-base) MAPSORT of: KIBB8239.seq  Check: 1423  from: 1  to: 423



KIBB8239.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:28  ..

ApaI G_GGCC'C

Cuts at:      0      49     423 
   Size:         49     374
 
BanII G_rGCy'C

Cuts at:      0      49     420     423 
   Size:         49     371       3

  Fragments arranged by size: 

                 371      49       3
 
BglI GCCn_nnn'nGGC

Cuts at:      0      61     423 
   Size:         61     362
 
Bme1580I G_kGCm'C

Cuts at:      0      49     423 
   Size:         49     374
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      24     423 
   Size:         24     399
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     423 
   Size:        244     179
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      95     125     390     420     423 
   Size:         95      30     265      30       3

  Fragments arranged by size: 

                 265      95      30      30       3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     116     211     423 
   Size:        116      95     212

  Fragments arranged by size: 

                 212     116      95
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     423 
   Size:        187     236
 
BsiEI CG_ry'CG

Cuts at:      0      56     423 
   Size:         56     367
 
BsiHKAI G_wGCw'C

Cuts at:      0     405     423 
   Size:        405      18
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      90     423 
   Size:         90     333
 
Bsp1286I G_dGCh'C

Cuts at:      0      49     405     420     423 
   Size:         49     356      15       3

  Fragments arranged by size: 

                 356      49      15       3
 
BsrBI CCG'CTC

Cuts at:      0     332     375     423 
   Size:        332      43      48

  Fragments arranged by size: 

                 332      48      43
 
BssSI C'ACGA_G

Cuts at:      0     404     423 
   Size:        404      19
 
BstEII G'GTnAC_C

Cuts at:      0     216     423 
   Size:        216     207
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     361     387     423 
   Size:        361      26      36

  Fragments arranged by size: 

                 361      36      26
 
EaeI y'GGCC_r

Cuts at:      0       2      53     138     173     284     423 
   Size:          2      51      85      35     111     139

  Fragments arranged by size: 

                 139     111      85      51      35       2
 
EagI C'GGCC_G

Cuts at:      0      53     423 
   Size:         53     370
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     423 
   Size:        244     179
 
EcoO109I rG'GnC_Cy

Cuts at:      0      45      46     279     423 
   Size:         45       1     233     144

  Fragments arranged by size: 

                 233     144      45       1
 
FspI TGC'GCA

Cuts at:      0      37     423 
   Size:         37     386
 
MscI TGG'CCA

Cuts at:      0     140     286     423 
   Size:        140     146     137

  Fragments arranged by size: 

                 146     140     137
 
MslI CAynn'nnrTG

Cuts at:      0     385     423 
   Size:        385      38
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     128     423 
   Size:        128     295
 
PpuMI rG'GwC_Cy

Cuts at:      0     279     423 
   Size:        279     144
 
PspOMI G'GGCC_C

Cuts at:      0      45     423 
   Size:         45     378
 
SexAI A'CCwGG_T

Cuts at:      0     353     423 
   Size:        353      70
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      61     423 
   Size:         61     362
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      95     127     390     422     423 
   Size:         95      32     263      32       1

  Fragments arranged by size: 

                 263      95      32      32       1
 

 Enzymes that do cut:

     ApaI    BanII     BglI Bme1580I     BmrI     BpmI    BsaXI    BseRI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BsrBI    BssSI   BstEII
    BstXI     EaeI     EagI  Eco57MI EcoO109I     FspI     MscI     MslI
    PflMI    PpuMI   PspOMI    SexAI     SfiI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseYI    BsiWI     BsmI    BspEI
    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EarI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI