Restriction map for
pFN21AB8130
(Linear) (Six-base) MAPSORT of: KIBB8130.seq Check: 6784 from: 1 to: 1341
KIBB8130.orf
With 182 enzymes: SgfI *
July 15, 2010 18:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 435 1341
Size: 435 906
AatII G_ACGT'C
Cuts at: 0 439 1341
Size: 439 902
AccI GT'mk_AC
Cuts at: 0 716 1341
Size: 716 625
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 478 1341
Size: 415 63 863
Fragments arranged by size:
863 415 63
AflII C'TTAA_G
Cuts at: 0 415 1341
Size: 415 926
AflIII A'CryG_T
Cuts at: 0 203 563 1341
Size: 203 360 778
Fragments arranged by size:
778 360 203
AlwNI CAG_nnn'CTG
Cuts at: 0 551 682 1341
Size: 551 131 659
Fragments arranged by size:
659 551 131
AvaI C'yCGr_G
Cuts at: 0 893 1131 1151 1341
Size: 893 238 20 190
Fragments arranged by size:
893 238 190 20
BanI G'GyrC_C
Cuts at: 0 447 596 1259 1341
Size: 447 149 663 82
Fragments arranged by size:
663 447 149 82
BanII G_rGCy'C
Cuts at: 0 490 736 1341
Size: 490 246 605
Fragments arranged by size:
605 490 246
BbsI GAAGACnn'nnnn_
Cuts at: 0 67 1341
Size: 67 1274
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 693 727 1341
Size: 693 34 614
Fragments arranged by size:
693 614 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 379 1341
Size: 379 962
BclI T'GATC_A
Cuts at: 0 671 1341
Size: 671 670
BglI GCCn_nnn'nGGC
Cuts at: 0 875 1341
Size: 875 466
BmgBI CAC'GTC
Cuts at: 0 204 858 1116 1341
Size: 204 654 258 225
Fragments arranged by size:
654 258 225 204
BmrI ACTGGGnnnn_n'
Cuts at: 0 806 1341
Size: 806 535
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 277 1144 1341
Size: 50 227 867 197
Fragments arranged by size:
867 227 197 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 1147 1341
Size: 232 915 194
Fragments arranged by size:
915 232 194
BsaI GGTCTCn'nnnn_
Cuts at: 0 550 943 1341
Size: 550 393 398
Fragments arranged by size:
550 398 393
BsaHI Gr'CG_yC
Cuts at: 0 436 1341
Size: 436 905
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 514 520 544 550 1341
Size: 514 6 24 6 791
Fragments arranged by size:
791 514 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 280 834 1341
Size: 280 554 507
Fragments arranged by size:
554 507 280
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1231 1341
Size: 1231 110
BsiEI CG_ry'CG
Cuts at: 0 138 879 1098 1341
Size: 138 741 219 243
Fragments arranged by size:
741 243 219 138
BsiHKAI G_wGCw'C
Cuts at: 0 1163 1341
Size: 1163 178
BsmI GAATG_Cn'
Cuts at: 0 780 1341
Size: 780 561
Bsp1286I G_dGCh'C
Cuts at: 0 490 736 1163 1341
Size: 490 246 427 178
Fragments arranged by size:
490 427 246 178
BspHI T'CATG_A
Cuts at: 0 1058 1341
Size: 1058 283
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 435 587 1341
Size: 435 152 754
Fragments arranged by size:
754 435 152
BsrBI CCG'CTC
Cuts at: 0 821 1341
Size: 821 520
BsrDI GCAATG_nn'
Cuts at: 0 1277 1341
Size: 1277 64
BsrFI r'CCGG_y
Cuts at: 0 642 1196 1341
Size: 642 554 145
Fragments arranged by size:
642 554 145
BsrGI T'GTAC_A
Cuts at: 0 206 1304 1341
Size: 206 1098 37
Fragments arranged by size:
1098 206 37
BssSI C'ACGA_G
Cuts at: 0 226 1341
Size: 226 1115
BstYI r'GATC_y
Cuts at: 0 468 497 1241 1341
Size: 468 29 744 100
Fragments arranged by size:
744 468 100 29
Bsu36I CC'TnA_GG
Cuts at: 0 803 1341
Size: 803 538
BtgI C'CryG_G
Cuts at: 0 428 464 1168 1341
Size: 428 36 704 173
Fragments arranged by size:
704 428 173 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1269 1341
Size: 1269 72
BtsI GCAGTG_nn'
Cuts at: 0 439 699 1341
Size: 439 260 642
Fragments arranged by size:
642 439 260
DrdI GACnn_nn'nnGTC
Cuts at: 0 564 1341
Size: 564 777
EaeI y'GGCC_r
Cuts at: 0 116 135 510 602 644 876 1341
Size: 116 19 375 92 42 232 465
Fragments arranged by size:
465 375 232 116 92 42 19
EagI C'GGCC_G
Cuts at: 0 135 876 1341
Size: 135 741 465
Fragments arranged by size:
741 465 135
EarI CTCTTCn'nnn_
Cuts at: 0 398 715 791 1341
Size: 398 317 76 550
Fragments arranged by size:
550 398 317 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 586 1341
Size: 586 755
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 277 415 478 1144 1341
Size: 50 227 138 63 666 197
Fragments arranged by size:
666 227 197 138 63 50
EcoNI CCTnn'n_nnAGG
Cuts at: 0 664 1341
Size: 664 677
EcoO109I rG'GnC_Cy
Cuts at: 0 420 657 807 921 1067 1341
Size: 420 237 150 114 146 274
Fragments arranged by size:
420 274 237 150 146 114
FspI TGC'GCA
Cuts at: 0 583 1341
Size: 583 758
HaeII r_GCGC'y
Cuts at: 0 1197 1341
Size: 1197 144
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 64 96 1341
Size: 64 32 1245
Fragments arranged by size:
1245 64 32
HincII GTy'rAC
Cuts at: 0 543 1341
Size: 543 798
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 878 1341
Size: 878 463
MscI TGG'CCA
Cuts at: 0 118 512 604 1341
Size: 118 394 92 737
Fragments arranged by size:
737 394 118 92
MslI CAynn'nnrTG
Cuts at: 0 1213 1341
Size: 1213 128
MspA1I CmG'CkG
Cuts at: 0 70 156 763 875 1341
Size: 70 86 607 112 466
Fragments arranged by size:
607 466 112 86 70
NaeI GCC'GGC
Cuts at: 0 644 1198 1341
Size: 644 554 143
Fragments arranged by size:
644 554 143
NcoI C'CATG_G
Cuts at: 0 464 1341
Size: 464 877
NgoMIV G'CCGG_C
Cuts at: 0 642 1196 1341
Size: 642 554 145
Fragments arranged by size:
642 554 145
NotI GC'GGCC_GC
Cuts at: 0 876 1341
Size: 876 465
NspI r_CATG'y
Cuts at: 0 567 756 888 1092 1338 1341
Size: 567 189 132 204 246 3
Fragments arranged by size:
567 246 204 189 132 3
PasI CC'CwG_GG
Cuts at: 0 50 1341
Size: 50 1291
PciI A'CATG_T
Cuts at: 0 563 1341
Size: 563 778
PfoI T'CCnGG_A
Cuts at: 0 35 1313 1341
Size: 35 1278 28
Fragments arranged by size:
1278 35 28
PpuMI rG'GwC_Cy
Cuts at: 0 420 657 807 921 1341
Size: 420 237 150 114 420
Fragments arranged by size:
420 420 237 150 114
PspXI vC'TCGA_Gb
Cuts at: 0 1131 1341
Size: 1131 210
PstI C_TGCA'G
Cuts at: 0 446 1341
Size: 446 895
PvuI CG_AT'CG
Cuts at: 0 1098 1341
Size: 1098 243
PvuII CAG'CTG
Cuts at: 0 70 763 1341
Size: 70 693 578
Fragments arranged by size:
693 578 70
SapI GCTCTTCn'nnn_
Cuts at: 0 791 1341
Size: 791 550
SexAI A'CCwGG_T
Cuts at: 0 956 1341
Size: 956 385
SfcI C'TryA_G
Cuts at: 0 442 717 1341
Size: 442 275 624
Fragments arranged by size:
624 442 275
SgrAI Cr'CCGG_yG
Cuts at: 0 1196 1341
Size: 1196 145
SmaI CCC'GGG
Cuts at: 0 895 1153 1341
Size: 895 258 188
Fragments arranged by size:
895 258 188
SmlI C'TyrA_G
Cuts at: 0 247 415 1131 1162 1341
Size: 247 168 716 31 179
Fragments arranged by size:
716 247 179 168 31
StuI AGG'CCT
Cuts at: 0 1129 1341
Size: 1129 212
StyI C'CwwG_G
Cuts at: 0 464 1341
Size: 464 877
TatI w'GTAC_w
Cuts at: 0 206 1304 1341
Size: 206 1098 37
Fragments arranged by size:
1098 206 37
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 514 546 1341
Size: 514 32 795
Fragments arranged by size:
795 514 32
Tth111I GACn'n_nGTC
Cuts at: 0 1051 1341
Size: 1051 290
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 471 1341
Size: 471 870
XhoI C'TCGA_G
Cuts at: 0 1131 1341
Size: 1131 210
XmaI C'CCGG_G
Cuts at: 0 893 1151 1341
Size: 893 258 190
Fragments arranged by size:
893 258 190
ZraI GAC'GTC
Cuts at: 0 437 1341
Size: 437 904
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AlwNI AvaI
BanI BanII BbsI BcgI BciVI BclI BglI BmgBI
BmrI BpmI BpuEI BsaI BsaHI BsaXI BseRI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstYI Bsu36I BtgI BtgZI BtsI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
FspI HaeII Hin4I HincII MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NotI NspI PasI PciI PfoI
PpuMI PspXI PstI PvuI PvuII SapI SexAI SfcI
SgrAI SmaI SmlI StuI StyI TatI TstI Tth111I
XcmI XhoI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AgeI AhdI AleI AlfI AloI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BfrBI BglII BlpI Bme1580I BmtI
BplI Bpu10I BsaAI BsaBI BsaWI BseYI BsiWI BsmBI
BspEI BssHII BstAPI BstBI BstEII BstXI BstZ17I ClaI
CspCI DraI DraIII EcoICRI EcoRI EcoRV FalI FseI
FspAI HindIII HpaI KasI KpnI MfeI MluI NarI
NdeI NheI NruI NsiI PacI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PsrI RsrII SacI SacII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SnaBI
SpeI SphI SrfI SspI SwaI TaqII XbaI XmnI