(Linear) (Six-base) MAPSORT of: KIBB8118.seq  Check: 8369  from: 1  to: 534



KIBB8118.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:28  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89     182     534 
   Size:         89      93     352

  Fragments arranged by size: 

                 352      93      89
 
AfeI AGC'GCT

Cuts at:      0     144     534 
   Size:        144     390
 
AlwNI CAG_nnn'CTG

Cuts at:      0     480     534 
   Size:        480      54
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     405     534 
   Size:        405     129
 
BglII A'GATC_T

Cuts at:      0     200     534 
   Size:        200     334
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71     359     534 
   Size:         71     288     175

  Fragments arranged by size: 

                 288     175      71
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     291     321     534 
   Size:        291      30     213

  Fragments arranged by size: 

                 291     213      30
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      14     534 
   Size:         14     520
 
BstYI r'GATC_y

Cuts at:      0     200     534 
   Size:        200     334
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     534 
   Size:         19     515
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71      89     182     359     534 
   Size:         71      18      93     177     175

  Fragments arranged by size: 

                 177     175      93      71      18
 
EcoO109I rG'GnC_Cy

Cuts at:      0      64     534 
   Size:         64     470
 
HaeII r_GCGC'y

Cuts at:      0     146     534 
   Size:        146     388
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     139     171     534 
   Size:        139      32     363

  Fragments arranged by size: 

                 363     139      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     534 
   Size:        322     212
 
MspA1I CmG'CkG

Cuts at:      0     160     427     534 
   Size:        160     267     107

  Fragments arranged by size: 

                 267     160     107
 
PpuMI rG'GwC_Cy

Cuts at:      0      64     534 
   Size:         64     470
 
PvuII CAG'CTG

Cuts at:      0     160     427     534 
   Size:        160     267     107

  Fragments arranged by size: 

                 267     160     107
 
SspI AAT'ATT

Cuts at:      0     402     534 
   Size:        402     132
 
XmnI GAAnn'nnTTC

Cuts at:      0     293     534 
   Size:        293     241
 

 Enzymes that do cut:

     AcuI     AfeI    AlwNI    BciVI    BglII     BpmI    BsaXI    BsmBI
    BstYI     EciI  Eco57MI EcoO109I    HaeII    Hin4I     MmeI   MspA1I
    PpuMI    PvuII     SspI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI