Restriction map for
pFN21AB8028
(Linear) (Six-base) MAPSORT of: KIBB8028.seq Check: 768 from: 1 to: 3312
KIBB8028.orf
With 182 enzymes: SgfI *
July 15, 2010 18:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 450 3312
Size: 450 2862
AccI GT'mk_AC
Cuts at: 0 788 2978 3312
Size: 788 2190 334
Fragments arranged by size:
2190 788 334
Acc65I G'GTAC_C
Cuts at: 0 98 3312
Size: 98 3214
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 1190 2055 2396 3312
Size: 124 1066 865 341 916
Fragments arranged by size:
1066 916 865 341 124
AfeI AGC'GCT
Cuts at: 0 462 3312
Size: 462 2850
AflIII A'CryG_T
Cuts at: 0 645 3312
Size: 645 2667
AhdI GACnn_n'nnGTC
Cuts at: 0 2397 3312
Size: 2397 915
AleI CACnn'nnGTG
Cuts at: 0 473 3312
Size: 473 2839
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 55 418 3312
Size: 55 363 2894
Fragments arranged by size:
2894 363 55
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 996 1028 3312
Size: 996 32 2284
Fragments arranged by size:
2284 996 32
AlwNI CAG_nnn'CTG
Cuts at: 0 90 486 1516 2974 3312
Size: 90 396 1030 1458 338
Fragments arranged by size:
1458 1030 396 338 90
ApaI G_GGCC'C
Cuts at: 0 3236 3312
Size: 3236 76
ApaLI G'TGCA_C
Cuts at: 0 1454 3312
Size: 1454 1858
ApoI r'AATT_y
Cuts at: 0 1295 2460 3312
Size: 1295 1165 852
Fragments arranged by size:
1295 1165 852
AseI AT'TA_AT
Cuts at: 0 2108 3312
Size: 2108 1204
AvaI C'yCGr_G
Cuts at: 0 110 1058 1424 1525 2999 3312
Size: 110 948 366 101 1474 313
Fragments arranged by size:
1474 948 366 313 110 101
BamHI G'GATC_C
Cuts at: 0 177 1791 3312
Size: 177 1614 1521
Fragments arranged by size:
1614 1521 177
BanI G'GyrC_C
Cuts at: 0 39 98 2617 2877 3312
Size: 39 59 2519 260 435
Fragments arranged by size:
2519 435 260 59 39
BanII G_rGCy'C
Cuts at: 0 77 271 301 2413 2433 3008 3045 3092
Size: 77 194 30 2112 20 575 37 47
Cuts at: 3092 3236 3312
Size: 144 76
Fragments arranged by size:
2112 575 194 144 77 76 47 37
30 20
BbeI G_GCGC'C
Cuts at: 0 2621 3312
Size: 2621 691
BbsI GAAGACnn'nnnn_
Cuts at: 0 112 530 716 810 2837 3312
Size: 112 418 186 94 2027 475
Fragments arranged by size:
2027 475 418 186 112 94
BbvCI CC'TCA_GC
Cuts at: 0 3116 3312
Size: 3116 196
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2296 2330 3312
Size: 2296 34 982
Fragments arranged by size:
2296 982 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1888 2782 3122 3312
Size: 1888 894 340 190
Fragments arranged by size:
1888 894 340 190
BfrBI ATG'CAT
Cuts at: 0 1027 3312
Size: 1027 2285
BglI GCCn_nnn'nGGC
Cuts at: 0 334 3312
Size: 334 2978
BglII A'GATC_T
Cuts at: 0 2074 3102 3312
Size: 2074 1028 210
Fragments arranged by size:
2074 1028 210
BlpI GC'TnA_GC
Cuts at: 0 2666 3312
Size: 2666 646
Bme1580I G_kGCm'C
Cuts at: 0 44 656 1458 3236 3312
Size: 44 612 802 1778 76
Fragments arranged by size:
1778 802 612 76 44
BmrI ACTGGGnnnn_n'
Cuts at: 0 141 266 421 3312
Size: 141 125 155 2891
Fragments arranged by size:
2891 155 141 125
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1486 2734 3312
Size: 1486 1248 578
Fragments arranged by size:
1486 1248 578
Bpu10I CC'TnA_GC
Cuts at: 0 803 1357 3091 3116 3312
Size: 803 554 1734 25 196
Fragments arranged by size:
1734 803 554 196 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 794 3312
Size: 794 2518
BsaI GGTCTCn'nnnn_
Cuts at: 0 1773 3312
Size: 1773 1539
BsaAI yAC'GTr
Cuts at: 0 858 3312
Size: 858 2454
BsaBI GATnn'nnATC
Cuts at: 0 1582 3312
Size: 1582 1730
BsaHI Gr'CG_yC
Cuts at: 0 2618 3312
Size: 2618 694
BsaWI w'CCGG_w
Cuts at: 0 1324 3107 3312
Size: 1324 1783 205
Fragments arranged by size:
1783 1324 205
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 405 435 2799 2829 2897 2923 2927 2953
Size: 405 30 2364 30 68 26 4 26
Cuts at: 2953 3312
Size: 359
Fragments arranged by size:
2364 405 359 68 30 30 26 26
4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 426 841 1782 2587 3312
Size: 426 415 941 805 725
Fragments arranged by size:
941 805 725 426 415
BseYI C'CCAG_C
Cuts at: 0 80 452 1071 3143 3188 3312
Size: 80 372 619 2072 45 124
Fragments arranged by size:
2072 619 372 124 80 45
BsiEI CG_ry'CG
Cuts at: 0 2305 3312
Size: 2305 1007
BsiHKAI G_wGCw'C
Cuts at: 0 77 851 1366 1458 2413 2433 3092 3312
Size: 77 774 515 92 955 20 659 220
Fragments arranged by size:
955 774 659 515 220 92 77 20
BsmI GAATG_Cn'
Cuts at: 0 2907 3206 3312
Size: 2907 299 106
Fragments arranged by size:
2907 299 106
Bsp1286I G_dGCh'C
Cuts at: 0 44 77 271 301 656 851 1366 1458
Size: 44 33 194 30 355 195 515 92
Cuts at: 1458 2413 2433 3008 3045 3092 3236 3312
Size: 955 20 575 37 47 144 76
Fragments arranged by size:
955 575 515 355 195 194 144 92
76 47 44 37 33 30 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 450 710 3312
Size: 450 260 2602
Fragments arranged by size:
2602 450 260
BsrDI GCAATG_nn'
Cuts at: 0 836 1394 2337 3312
Size: 836 558 943 975
Fragments arranged by size:
975 943 836 558
BsrFI r'CCGG_y
Cuts at: 0 2085 3312
Size: 2085 1227
BsrGI T'GTAC_A
Cuts at: 0 546 3312
Size: 546 2766
BstAPI GCAn_nnn'nTGC
Cuts at: 0 90 3312
Size: 90 3222
BstEII G'GTnAC_C
Cuts at: 0 1191 3312
Size: 1191 2121
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2026 2238 3312
Size: 2026 212 1074
Fragments arranged by size:
2026 1074 212
BstYI r'GATC_y
Cuts at: 0 177 780 1493 1791 2074 2583 2970 3102
Size: 177 603 713 298 283 509 387 132
Cuts at: 3102 3312
Size: 210
Fragments arranged by size:
713 603 509 387 298 283 210 177
132
Bsu36I CC'TnA_GG
Cuts at: 0 2453 3312
Size: 2453 859
BtgI C'CryG_G
Cuts at: 0 966 1724 3237 3312
Size: 966 758 1513 75
Fragments arranged by size:
1513 966 758 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1370 3312
Size: 1370 1942
BtsI GCAGTG_nn'
Cuts at: 0 400 426 2264 3312
Size: 400 26 1838 1048
Fragments arranged by size:
1838 1048 400 26
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 590 625 3312
Size: 590 35 2687
Fragments arranged by size:
2687 590 35
EaeI y'GGCC_r
Cuts at: 0 323 335 477 1272 1349 2087 2261 2302
Size: 323 12 142 795 77 738 174 41
Cuts at: 2302 2421 2742 3312
Size: 119 321 570
Fragments arranged by size:
795 738 570 323 321 174 142 119
77 41 12
EagI C'GGCC_G
Cuts at: 0 2302 3312
Size: 2302 1010
EarI CTCTTCn'nnn_
Cuts at: 0 697 1209 1334 2119 2702 2723 2838 3312
Size: 697 512 125 785 583 21 115 474
Fragments arranged by size:
785 697 583 512 474 125 115 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 811 3164 3312
Size: 811 2353 148
Fragments arranged by size:
2353 811 148
EcoICRI GAG'CTC
Cuts at: 0 75 2411 2431 3090 3312
Size: 75 2336 20 659 222
Fragments arranged by size:
2336 659 222 75 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 1190 1486 2055 2396 2734 3312
Size: 124 1066 296 569 341 338 578
Fragments arranged by size:
1066 578 569 341 338 296 124
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2292 2952 3062 3312
Size: 2292 660 110 250
Fragments arranged by size:
2292 660 250 110
EcoO109I rG'GnC_Cy
Cuts at: 0 121 1165 2297 2962 3154 3232 3233 3312
Size: 121 1044 1132 665 192 78 1 79
Fragments arranged by size:
1132 1044 665 192 121 79 78 1
EcoRI G'AATT_C
Cuts at: 0 2460 3312
Size: 2460 852
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1842 2628 3312
Size: 1842 786 684
Fragments arranged by size:
1842 786 684
HaeII r_GCGC'y
Cuts at: 0 91 464 1610 2621 3312
Size: 91 373 1146 1011 691
Fragments arranged by size:
1146 1011 691 373 91
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 680 712 978 1010 1369 1401 1733 1765
Size: 680 32 266 32 359 32 332 32
Cuts at: 1765 1988 2020 2383 2395 2415 2427 3312
Size: 223 32 363 12 20 12 885
Fragments arranged by size:
885 680 363 359 332 266 223 32
32 32 32 32 20 12 12
KasI G'GCGC_C
Cuts at: 0 2617 3312
Size: 2617 695
KpnI G_GTAC'C
Cuts at: 0 102 3312
Size: 102 3210
MfeI C'AATT_G
Cuts at: 0 350 3312
Size: 350 2962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1709 1999 2233 3312
Size: 1709 290 234 1079
Fragments arranged by size:
1709 1079 290 234
MscI TGG'CCA
Cuts at: 0 479 1351 2423 3312
Size: 479 872 1072 889
Fragments arranged by size:
1072 889 872 479
MslI CAynn'nnrTG
Cuts at: 0 37 473 749 3312
Size: 37 436 276 2563
Fragments arranged by size:
2563 436 276 37
MspA1I CmG'CkG
Cuts at: 0 60 96 1647 3312
Size: 60 36 1551 1665
Fragments arranged by size:
1665 1551 60 36
NarI GG'CG_CC
Cuts at: 0 2618 3312
Size: 2618 694
NcoI C'CATG_G
Cuts at: 0 966 1724 3312
Size: 966 758 1588
Fragments arranged by size:
1588 966 758
NsiI A_TGCA'T
Cuts at: 0 1029 3312
Size: 1029 2283
NspI r_CATG'y
Cuts at: 0 649 3312
Size: 649 2663
PasI CC'CwG_GG
Cuts at: 0 2954 3312
Size: 2954 358
PciI A'CATG_T
Cuts at: 0 645 3312
Size: 645 2667
PflMI CCAn_nnn'nTGG
Cuts at: 0 355 3312
Size: 355 2957
PfoI T'CCnGG_A
Cuts at: 0 173 3312
Size: 173 3139
PmlI CAC'GTG
Cuts at: 0 858 3312
Size: 858 2454
PpuMI rG'GwC_Cy
Cuts at: 0 121 2962 3154 3312
Size: 121 2841 192 158
Fragments arranged by size:
2841 192 158 121
PspOMI G'GGCC_C
Cuts at: 0 3232 3312
Size: 3232 80
PstI C_TGCA'G
Cuts at: 0 95 288 1800 2252 2259 3306 3312
Size: 95 193 1512 452 7 1047 6
Fragments arranged by size:
1512 1047 452 193 95 7 6
PvuII CAG'CTG
Cuts at: 0 60 1647 3312
Size: 60 1587 1665
Fragments arranged by size:
1665 1587 60
SacI G_AGCT'C
Cuts at: 0 77 2413 2433 3092 3312
Size: 77 2336 20 659 220
Fragments arranged by size:
2336 659 220 77 20
SapI GCTCTTCn'nnn_
Cuts at: 0 1334 2119 2702 2723 2838 3312
Size: 1334 785 583 21 115 474
Fragments arranged by size:
1334 785 583 474 115 21
SfcI C'TryA_G
Cuts at: 0 91 284 1796 2248 2255 2975 3302 3312
Size: 91 193 1512 452 7 720 327 10
Fragments arranged by size:
1512 720 452 327 193 91 10 7
SfoI GGC'GCC
Cuts at: 0 2619 3312
Size: 2619 693
SmaI CCC'GGG
Cuts at: 0 112 3312
Size: 112 3200
SmlI C'TyrA_G
Cuts at: 0 809 3312
Size: 809 2503
StyI C'CwwG_G
Cuts at: 0 966 1066 1724 2237 2424 3312
Size: 966 100 658 513 187 888
Fragments arranged by size:
966 888 658 513 187 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 94 123 2865 3312
Size: 94 29 2742 447
Fragments arranged by size:
2742 447 94 29
TatI w'GTAC_w
Cuts at: 0 546 3312
Size: 546 2766
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 405 437 1887 1919 2897 2929 3312
Size: 405 32 1450 32 978 32 383
Fragments arranged by size:
1450 978 405 383 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 516 3312
Size: 516 2796
XmaI C'CCGG_G
Cuts at: 0 110 3312
Size: 110 3202
XmnI GAAnn'nnTTC
Cuts at: 0 2611 3312
Size: 2611 701
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI
BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I KasI KpnI
MfeI MmeI MscI MslI MspA1I NarI NcoI NsiI
NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI
PstI PvuII SacI SapI SfcI SfoI SmaI SmlI
StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AscI AsiSI AvrII BaeI
BclI BmgBI BmtI BplI BsgI BsiWI BsmBI BspEI
BspHI BsrBI BssHII BssSI BstBI BstZ17I ClaI DraI
DraIII DrdI EcoRV FseI FspI FspAI HincII HindIII
HpaI MluI NaeI NdeI NgoMIV NheI NotI NruI
PacI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XhoI ZraI