Restriction map for
pFN21AB7963
(Linear) (Six-base) MAPSORT of: KIBB7963.seq Check: 1114 from: 1 to: 876
KIBB7963.orf
With 182 enzymes: SgfI *
March 22, 2012 15:43 ..
AccI GT'mk_AC
Cuts at: 0 13 876
Size: 13 863
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 572 583 848 876
Size: 259 313 11 265 28
Fragments arranged by size:
313 265 259 28 11
AfeI AGC'GCT
Cuts at: 0 499 876
Size: 499 377
AhdI GACnn_n'nnGTC
Cuts at: 0 846 876
Size: 846 30
ApoI r'AATT_y
Cuts at: 0 190 876
Size: 190 686
AvaI C'yCGr_G
Cuts at: 0 346 876
Size: 346 530
BanI G'GyrC_C
Cuts at: 0 724 876
Size: 724 152
BbsI GAAGACnn'nnnn_
Cuts at: 0 334 679 876
Size: 334 345 197
Fragments arranged by size:
345 334 197
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 426 460 876
Size: 426 34 416
Fragments arranged by size:
426 416 34
BglI GCCn_nnn'nGGC
Cuts at: 0 482 876
Size: 482 394
BglII A'GATC_T
Cuts at: 0 210 876
Size: 210 666
BmrI ACTGGGnnnn_n'
Cuts at: 0 873 876
Size: 873 3
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 361 876
Size: 361 515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 876
Size: 451 425
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 202 876
Size: 39 163 674
Fragments arranged by size:
674 163 39
BsaI GGTCTCn'nnnn_
Cuts at: 0 346 691 876
Size: 346 345 185
Fragments arranged by size:
346 345 185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 876
Size: 487 389
BsiHKAI G_wGCw'C
Cuts at: 0 858 876
Size: 858 18
BsmI GAATG_Cn'
Cuts at: 0 746 876
Size: 746 130
Bsp1286I G_dGCh'C
Cuts at: 0 858 876
Size: 858 18
BspHI T'CATG_A
Cuts at: 0 858 876
Size: 858 18
BsrDI GCAATG_nn'
Cuts at: 0 98 876
Size: 98 778
BsrFI r'CCGG_y
Cuts at: 0 259 288 633 876
Size: 259 29 345 243
Fragments arranged by size:
345 259 243 29
BssSI C'ACGA_G
Cuts at: 0 523 876
Size: 523 353
BstYI r'GATC_y
Cuts at: 0 210 876
Size: 210 666
BtgI C'CryG_G
Cuts at: 0 74 299 387 419 644 876
Size: 74 225 88 32 225 232
Fragments arranged by size:
232 225 225 88 74 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 429 876
Size: 429 447
BtsI GCAGTG_nn'
Cuts at: 0 783 876
Size: 783 93
EaeI y'GGCC_r
Cuts at: 0 77 422 527 641 876
Size: 77 345 105 114 235
Fragments arranged by size:
345 235 114 105 77
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 163 508 876
Size: 163 345 368
Fragments arranged by size:
368 345 163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 451 572 583 848 876
Size: 259 192 121 11 265 28
Fragments arranged by size:
265 259 192 121 28 11
EcoO109I rG'GnC_Cy
Cuts at: 0 43 876
Size: 43 833
HaeII r_GCGC'y
Cuts at: 0 501 876
Size: 501 375
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 131 163 506 538 832 864 876
Size: 131 32 343 32 294 32 12
Fragments arranged by size:
343 294 131 32 32 32 12
HincII GTy'rAC
Cuts at: 0 234 840 876
Size: 234 606 36
Fragments arranged by size:
606 234 36
HindIII A'AGCT_T
Cuts at: 0 200 876
Size: 200 676
MscI TGG'CCA
Cuts at: 0 79 424 529 643 876
Size: 79 345 105 114 233
Fragments arranged by size:
345 233 114 105 79
MslI CAynn'nnrTG
Cuts at: 0 776 876
Size: 776 100
MspA1I CmG'CkG
Cuts at: 0 126 876
Size: 126 750
NaeI GCC'GGC
Cuts at: 0 261 876
Size: 261 615
NcoI C'CATG_G
Cuts at: 0 74 299 419 644 876
Size: 74 225 120 225 232
Fragments arranged by size:
232 225 225 120 74
NgoMIV G'CCGG_C
Cuts at: 0 259 876
Size: 259 617
NspI r_CATG'y
Cuts at: 0 279 876
Size: 279 597
PasI CC'CwG_GG
Cuts at: 0 604 876
Size: 604 272
PflMI CCAn_nnn'nTGG
Cuts at: 0 305 650 876
Size: 305 345 226
Fragments arranged by size:
345 305 226
PstI C_TGCA'G
Cuts at: 0 366 876
Size: 366 510
SfcI C'TryA_G
Cuts at: 0 362 876
Size: 362 514
SmlI C'TyrA_G
Cuts at: 0 54 217 876
Size: 54 163 659
Fragments arranged by size:
659 163 54
StyI C'CwwG_G
Cuts at: 0 74 299 419 644 876
Size: 74 225 120 225 232
Fragments arranged by size:
232 225 225 120 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 605 876
Size: 605 271
TatI w'GTAC_w
Cuts at: 0 617 876
Size: 617 259
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 372 404 876
Size: 372 32 472
Fragments arranged by size:
472 372 32
Tth111I GACn'n_nGTC
Cuts at: 0 865 876
Size: 865 11
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 426 876
Size: 426 450
XmnI GAAnn'nnTTC
Cuts at: 0 169 212 514 876
Size: 169 43 302 362
Fragments arranged by size:
362 302 169 43
Enzymes that do cut:
AccI AcuI AfeI AhdI ApoI AvaI BanI BbsI
BcgI BglI BglII BmrI BplI BpmI BpuEI BsaI
BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrFI BssSI
BstYI BtgI BtgZI BtsI EaeI EciI Eco57MI EcoO109I
HaeII Hin4I HincII HindIII MscI MslI MspA1I NaeI
NcoI NgoMIV NspI PasI PflMI PstI SfcI SmlI
StyI TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanII BbeI BbvCI BciVI BclI
BfrBI BlpI Bme1580I BmgBI BmtI Bpu10I BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiWI BsmBI
BspEI BspMI BsrBI BsrGI BssHII BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI
EagI EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HpaI KasI KpnI MfeI MluI MmeI
NarI NdeI NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI XbaI XhoI XmaI ZraI