Restriction map for
pFN21AB7941
(Linear) (Six-base) MAPSORT of: KIBB7941.seq Check: 3376 from: 1 to: 1029
KIBB7941.orf
With 182 enzymes: SgfI *
March 22, 2012 15:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 660 905 1029
Size: 660 245 124
Fragments arranged by size:
660 245 124
Acc65I G'GTAC_C
Cuts at: 0 35 1029
Size: 35 994
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 1029
Size: 343 686
AflIII A'CryG_T
Cuts at: 0 875 1029
Size: 875 154
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 580 612 1029
Size: 580 32 417
Fragments arranged by size:
580 417 32
AlwNI CAG_nnn'CTG
Cuts at: 0 762 897 1029
Size: 762 135 132
Fragments arranged by size:
762 135 132
ApaI G_GGCC'C
Cuts at: 0 210 359 817 844 1029
Size: 210 149 458 27 185
Fragments arranged by size:
458 210 185 149 27
AvaI C'yCGr_G
Cuts at: 0 252 351 493 843 945 955 1029
Size: 252 99 142 350 102 10 74
Fragments arranged by size:
350 252 142 102 99 74 10
BanI G'GyrC_C
Cuts at: 0 35 483 1029
Size: 35 448 546
Fragments arranged by size:
546 448 35
BanII G_rGCy'C
Cuts at: 0 210 250 359 817 844 1029
Size: 210 40 109 458 27 185
Fragments arranged by size:
458 210 185 109 40 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 552 1029
Size: 552 477
BbvCI CC'TCA_GC
Cuts at: 0 793 1029
Size: 793 236
BglI GCCn_nnn'nGGC
Cuts at: 0 198 214 272 812 1029
Size: 198 16 58 540 217
Fragments arranged by size:
540 217 198 58 16
BglII A'GATC_T
Cuts at: 0 451 1029
Size: 451 578
Bme1580I G_kGCm'C
Cuts at: 0 176 210 359 488 817 844 1029
Size: 176 34 149 129 329 27 185
Fragments arranged by size:
329 185 176 149 129 34 27
BmgBI CAC'GTC
Cuts at: 0 570 801 1029
Size: 570 231 228
Fragments arranged by size:
570 231 228
BmrI ACTGGGnnnn_n'
Cuts at: 0 72 1029
Size: 72 957
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 413 1029
Size: 413 616
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 370 459 607 1029
Size: 93 277 89 148 422
Fragments arranged by size:
422 277 148 93 89
Bpu10I CC'TnA_GC
Cuts at: 0 793 1029
Size: 793 236
BsaBI GATnn'nnATC
Cuts at: 0 136 1029
Size: 136 893
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 232 262 580 610 865 895 1029
Size: 232 30 318 30 255 30 134
Fragments arranged by size:
318 255 232 134 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 7 339 844 847 850 1000 1009 1029
Size: 7 332 505 3 3 150 9 20
Fragments arranged by size:
505 332 150 20 9 7 3 3
BseYI C'CCAG_C
Cuts at: 0 375 465 651 853 890 1029
Size: 375 90 186 202 37 139
Fragments arranged by size:
375 202 186 139 90 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 1029
Size: 592 437
BsiEI CG_ry'CG
Cuts at: 0 630 1029
Size: 630 399
BsmBI CGTCTCn'nnnn_
Cuts at: 0 304 1029
Size: 304 725
Bsp1286I G_dGCh'C
Cuts at: 0 176 210 250 359 488 817 844 1029
Size: 176 34 40 109 129 329 27 185
Fragments arranged by size:
329 185 176 129 109 40 34 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 167 660 905 1029
Size: 167 493 245 124
Fragments arranged by size:
493 245 167 124
BssHII G'CGCG_C
Cuts at: 0 520 1029
Size: 520 509
BstAPI GCAn_nnn'nTGC
Cuts at: 0 762 1029
Size: 762 267
BstBI TT'CG_AA
Cuts at: 0 425 1029
Size: 425 604
BstXI CCAn_nnnn'nTGG
Cuts at: 0 117 1029
Size: 117 912
BstYI r'GATC_y
Cuts at: 0 126 451 1029
Size: 126 325 578
Fragments arranged by size:
578 325 126
BtgI C'CryG_G
Cuts at: 0 212 907 1029
Size: 212 695 122
Fragments arranged by size:
695 212 122
EaeI y'GGCC_r
Cuts at: 0 102 508 644 1029
Size: 102 406 136 385
Fragments arranged by size:
406 385 136 102
EarI CTCTTCn'nnn_
Cuts at: 0 821 986 1029
Size: 821 165 43
Fragments arranged by size:
821 165 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 272 723 1029
Size: 272 451 306
Fragments arranged by size:
451 306 272
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 343 370 459 607 1029
Size: 93 250 27 89 148 422
Fragments arranged by size:
422 250 148 93 89 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 121 1029
Size: 121 908
EcoO109I rG'GnC_Cy
Cuts at: 0 206 207 233 256 355 497 813 814
Size: 206 1 26 23 99 142 316 1
Cuts at: 814 840 903 1029
Size: 26 63 126
Fragments arranged by size:
316 206 142 126 99 63 26 26
23 1 1
HaeII r_GCGC'y
Cuts at: 0 586 1029
Size: 586 443
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 232 264 1029
Size: 232 32 765
Fragments arranged by size:
765 232 32
KpnI G_GTAC'C
Cuts at: 0 39 1029
Size: 39 990
MluI A'CGCG_T
Cuts at: 0 875 1029
Size: 875 154
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 590 908 1029
Size: 590 318 121
Fragments arranged by size:
590 318 121
MspA1I CmG'CkG
Cuts at: 0 513 576 909 924 1029
Size: 513 63 333 15 105
Fragments arranged by size:
513 333 105 63 15
NruI TCG'CGA
Cuts at: 0 752 1029
Size: 752 277
NspI r_CATG'y
Cuts at: 0 189 546 1029
Size: 189 357 483
Fragments arranged by size:
483 357 189
PasI CC'CwG_GG
Cuts at: 0 817 1029
Size: 817 212
PflMI CCAn_nnn'nTGG
Cuts at: 0 864 1029
Size: 864 165
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 580 612 1029
Size: 580 32 417
Fragments arranged by size:
580 417 32
PpuMI rG'GwC_Cy
Cuts at: 0 256 497 903 1029
Size: 256 241 406 126
Fragments arranged by size:
406 256 241 126
PspOMI G'GGCC_C
Cuts at: 0 206 355 813 840 1029
Size: 206 149 458 27 189
Fragments arranged by size:
458 206 189 149 27
PspXI vC'TCGA_Gb
Cuts at: 0 945 1029
Size: 945 84
SacII CC_GC'GG
Cuts at: 0 910 1029
Size: 910 119
SanDI GG'GwC_CC
Cuts at: 0 256 903 1029
Size: 256 647 126
Fragments arranged by size:
647 256 126
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 214 812 1029
Size: 214 598 217
Fragments arranged by size:
598 217 214
SmaI CCC'GGG
Cuts at: 0 254 495 845 957 1029
Size: 254 241 350 112 72
Fragments arranged by size:
350 254 241 112 72
SmlI C'TyrA_G
Cuts at: 0 945 1029
Size: 945 84
SphI G_CATG'C
Cuts at: 0 546 1029
Size: 546 483
SrfI GCCC'GGGC
Cuts at: 0 845 1029
Size: 845 184
StyI C'CwwG_G
Cuts at: 0 716 1029
Size: 716 313
TatI w'GTAC_w
Cuts at: 0 611 1029
Size: 611 418
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 865 897 1029
Size: 865 32 132
Fragments arranged by size:
865 132 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 117 814 861 1029
Size: 117 697 47 168
Fragments arranged by size:
697 168 117 47
XhoI C'TCGA_G
Cuts at: 0 945 1029
Size: 945 84
XmaI C'CCGG_G
Cuts at: 0 252 493 843 955 1029
Size: 252 241 350 112 74
Fragments arranged by size:
350 252 241 112 74
XmnI GAAnn'nnTTC
Cuts at: 0 595 1029
Size: 595 434
Enzymes that do cut:
AarI Acc65I AcuI AflIII AloI AlwNI ApaI AvaI
BanI BanII BbsI BbvCI BglI BglII Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BsaBI BsaXI BseRI BseYI
BsgI BsiEI BsmBI Bsp1286I BspMI BssHII BstAPI BstBI
BstXI BstYI BtgI EaeI EarI EciI Eco57MI EcoNI
EcoO109I HaeII Hin4I KpnI MluI MmeI MspA1I NruI
NspI PasI PflMI PpiI PpuMI PspOMI PspXI SacII
SanDI SfiI SmaI SmlI SphI SrfI StyI TatI
TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AleI
AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BcgI BciVI BclI BfrBI BlpI BmtI
BpuEI BsaI BsaAI BsaHI BsaWI BsiHKAI BsiWI BsmI
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssSI BstEII
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI MfeI MscI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
PacI PciI PfoI PmeI PmlI PshAI PsiI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SnaBI SpeI
SspI StuI SwaI TaqII Tth111I XbaI ZraI