Restriction map for
pFN21AB7933
(Linear) (Six-base) MAPSORT of: KIBB7933.seq Check: 268 from: 1 to: 1101
KIBB7933.orf
With 182 enzymes: SgfI *
July 3, 2012 16:32 ..
Acc65I G'GTAC_C
Cuts at: 0 566 1101
Size: 566 535
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 513 1101
Size: 235 278 588
Fragments arranged by size:
588 278 235
AflIII A'CryG_T
Cuts at: 0 1076 1101
Size: 1076 25
AhdI GACnn_n'nnGTC
Cuts at: 0 830 1101
Size: 830 271
ApaI G_GGCC'C
Cuts at: 0 791 1101
Size: 791 310
AvaI C'yCGr_G
Cuts at: 0 330 517 790 1101
Size: 330 187 273 311
Fragments arranged by size:
330 311 273 187
BamHI G'GATC_C
Cuts at: 0 676 990 1101
Size: 676 314 111
Fragments arranged by size:
676 314 111
BanI G'GyrC_C
Cuts at: 0 25 193 566 1101
Size: 25 168 373 535
Fragments arranged by size:
535 373 168 25
BanII G_rGCy'C
Cuts at: 0 239 502 524 634 791 801 1096 1101
Size: 239 263 22 110 157 10 295 5
Fragments arranged by size:
295 263 239 157 110 22 10 5
BbsI GAAGACnn'nnnn_
Cuts at: 0 100 763 1101
Size: 100 663 338
Fragments arranged by size:
663 338 100
BciVI GTATCCnnnnn_n'
Cuts at: 0 128 1101
Size: 128 973
BglI GCCn_nnn'nGGC
Cuts at: 0 334 421 519 895 1101
Size: 334 87 98 376 206
Fragments arranged by size:
376 334 206 98 87
BglII A'GATC_T
Cuts at: 0 740 1101
Size: 740 361
BlpI GC'TnA_GC
Cuts at: 0 49 584 1101
Size: 49 535 517
Fragments arranged by size:
535 517 49
Bme1580I G_kGCm'C
Cuts at: 0 28 196 791 1101
Size: 28 168 595 310
Fragments arranged by size:
595 310 168 28
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 660 772 1101
Size: 660 112 329
Fragments arranged by size:
660 329 112
Bpu10I CC'TnA_GC
Cuts at: 0 304 496 1101
Size: 304 192 605
Fragments arranged by size:
605 304 192
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 922 1101
Size: 922 179
BsaI GGTCTCn'nnnn_
Cuts at: 0 711 1101
Size: 711 390
BsaHI Gr'CG_yC
Cuts at: 0 601 1101
Size: 601 500
BsaWI w'CCGG_w
Cuts at: 0 361 730 1101
Size: 361 369 371
Fragments arranged by size:
371 369 361
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 135 138 159 360 363 366 390 1101
Size: 135 3 21 201 3 3 24 711
Fragments arranged by size:
711 201 135 24 21 3 3 3
BseYI C'CCAG_C
Cuts at: 0 535 1101
Size: 535 566
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 1057 1101
Size: 58 999 44
Fragments arranged by size:
999 58 44
BsiEI CG_ry'CG
Cuts at: 0 275 437 548 1101
Size: 275 162 111 553
Fragments arranged by size:
553 275 162 111
BsiHKAI G_wGCw'C
Cuts at: 0 1096 1101
Size: 1096 5
BsmI GAATG_Cn'
Cuts at: 0 40 1101
Size: 40 1061
Bsp1286I G_dGCh'C
Cuts at: 0 28 196 239 502 524 634 791 801
Size: 28 168 43 263 22 110 157 10
Cuts at: 801 1096 1101
Size: 295 5
Fragments arranged by size:
295 263 168 157 110 43 28 22
10 5
BspEI T'CCGG_A
Cuts at: 0 730 1101
Size: 730 371
BsrDI GCAATG_nn'
Cuts at: 0 23 1101
Size: 23 1078
BsrFI r'CCGG_y
Cuts at: 0 133 308 563 1101
Size: 133 175 255 538
Fragments arranged by size:
538 255 175 133
BssSI C'ACGA_G
Cuts at: 0 714 1101
Size: 714 387
BstBI TT'CG_AA
Cuts at: 0 764 1101
Size: 764 337
BstXI CCAn_nnnn'nTGG
Cuts at: 0 324 579 687 1101
Size: 324 255 108 414
Fragments arranged by size:
414 324 255 108
BstYI r'GATC_y
Cuts at: 0 676 740 990 1101
Size: 676 64 250 111
Fragments arranged by size:
676 250 111 64
BtgI C'CryG_G
Cuts at: 0 227 323 419 431 542 548 557 899
Size: 227 96 96 12 111 6 9 342
Cuts at: 899 1101
Size: 202
Fragments arranged by size:
342 227 202 111 96 96 12 9
6
EaeI y'GGCC_r
Cuts at: 0 65 83 224 272 434 470 545 551
Size: 65 18 141 48 162 36 75 6
Cuts at: 551 594 896 902 1101
Size: 43 302 6 199
Fragments arranged by size:
302 199 162 141 75 65 48 43
36 18 6 6
EagI C'GGCC_G
Cuts at: 0 272 434 545 1101
Size: 272 162 111 556
Fragments arranged by size:
556 272 162 111
EarI CTCTTCn'nnn_
Cuts at: 0 384 697 956 1101
Size: 384 313 259 145
Fragments arranged by size:
384 313 259 145
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 339 378 393 1101
Size: 339 39 15 708
Fragments arranged by size:
708 339 39 15
EcoICRI GAG'CTC
Cuts at: 0 1094 1101
Size: 1094 7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 513 660 772 1101
Size: 235 278 147 112 329
Fragments arranged by size:
329 278 235 147 112
EcoNI CCTnn'n_nnAGG
Cuts at: 0 671 1101
Size: 671 430
EcoO109I rG'GnC_Cy
Cuts at: 0 787 944 1101
Size: 787 157 157
Fragments arranged by size:
787 157 157
EcoRV GAT'ATC
Cuts at: 0 300 1101
Size: 300 801
HaeII r_GCGC'y
Cuts at: 0 542 936 1101
Size: 542 394 165
Fragments arranged by size:
542 394 165
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 725 757 816 848 982 1014 1081 1101
Size: 725 32 59 32 134 32 67 20
Fragments arranged by size:
725 134 67 59 32 32 32 20
KpnI G_GTAC'C
Cuts at: 0 570 1101
Size: 570 531
MluI A'CGCG_T
Cuts at: 0 1076 1101
Size: 1076 25
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 1101
Size: 668 433
MscI TGG'CCA
Cuts at: 0 898 1101
Size: 898 203
MslI CAynn'nnrTG
Cuts at: 0 322 1101
Size: 322 779
MspA1I CmG'CkG
Cuts at: 0 102 126 421 433 544 579 1023 1049
Size: 102 24 295 12 111 35 444 26
Cuts at: 1049 1101
Size: 52
Fragments arranged by size:
444 295 111 102 52 35 26 24
12
NaeI GCC'GGC
Cuts at: 0 135 310 1101
Size: 135 175 791
Fragments arranged by size:
791 175 135
NgoMIV G'CCGG_C
Cuts at: 0 133 308 1101
Size: 133 175 793
Fragments arranged by size:
793 175 133
NotI GC'GGCC_GC
Cuts at: 0 434 1101
Size: 434 667
PflMI CCAn_nnn'nTGG
Cuts at: 0 611 1101
Size: 611 490
PspOMI G'GGCC_C
Cuts at: 0 787 1101
Size: 787 314
PvuII CAG'CTG
Cuts at: 0 1023 1101
Size: 1023 78
SacI G_AGCT'C
Cuts at: 0 1096 1101
Size: 1096 5
SacII CC_GC'GG
Cuts at: 0 422 434 545 1101
Size: 422 12 111 556
Fragments arranged by size:
556 422 111 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 334 1101
Size: 334 767
SmaI CCC'GGG
Cuts at: 0 519 1101
Size: 519 582
SmlI C'TyrA_G
Cuts at: 0 937 1101
Size: 937 164
SrfI GCCC'GGGC
Cuts at: 0 519 1101
Size: 519 582
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 211 1101
Size: 211 890
XmaI C'CCGG_G
Cuts at: 0 517 1101
Size: 517 584
XmnI GAAnn'nnTTC
Cuts at: 0 760 1101
Size: 760 341
Enzymes that do cut:
Acc65I AcuI AflIII AhdI ApaI AvaI BamHI BanI
BanII BbsI BciVI BglI BglII BlpI Bme1580I BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BsrDI BsrFI BssSI
BstBI BstXI BstYI BtgI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I KpnI
MluI MmeI MscI MslI MspA1I NaeI NgoMIV NotI
PflMI PspOMI PvuII SacI SacII SfiI SmaI SmlI
SrfI TaqII XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BcgI BclI BfrBI BmgBI
BmrI BmtI BplI BsaAI BsaBI BsaXI BsiWI BsmBI
BspHI BspMI BsrBI BsrGI BssHII BstAPI BstEII BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EcoRI FalI FseI FspI FspAI HincII HindIII HpaI
KasI MfeI NarI NcoI NdeI NheI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SnaBI SpeI SphI SspI StuI StyI
SwaI TatI TstI Tth111I XbaI XcmI XhoI ZraI