Restriction map for
pFN21AB7931
(Linear) (Six-base) MAPSORT of: KIBB7931.seq Check: 7007 from: 1 to: 1095
KIBB7931.orf
With 182 enzymes: SgfI *
January 29, 2014 11:43 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 1095
Size: 461 634
AfeI AGC'GCT
Cuts at: 0 673 1095
Size: 673 422
AhdI GACnn_n'nnGTC
Cuts at: 0 435 1095
Size: 435 660
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 757 1095
Size: 757 338
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 304 336 1095
Size: 304 32 759
Fragments arranged by size:
759 304 32
ApaI G_GGCC'C
Cuts at: 0 196 231 340 1070 1095
Size: 196 35 109 730 25
Fragments arranged by size:
730 196 109 35 25
ApaLI G'TGCA_C
Cuts at: 0 1015 1095
Size: 1015 80
AvaI C'yCGr_G
Cuts at: 0 83 189 195 287 340 578 1095
Size: 83 106 6 92 53 238 517
Fragments arranged by size:
517 238 106 92 83 53 6
AvrII C'CTAG_G
Cuts at: 0 67 1095
Size: 67 1028
BanI G'GyrC_C
Cuts at: 0 51 132 1095
Size: 51 81 963
Fragments arranged by size:
963 81 51
BanII G_rGCy'C
Cuts at: 0 76 196 231 340 347 608 787 880
Size: 76 120 35 109 7 261 179 93
Cuts at: 880 949 1070 1095
Size: 69 121 25
Fragments arranged by size:
261 179 121 120 109 93 76 69
35 25 7
BbeI G_GCGC'C
Cuts at: 0 136 1095
Size: 136 959
BbsI GAAGACnn'nnnn_
Cuts at: 0 449 620 1095
Size: 449 171 475
Fragments arranged by size:
475 449 171
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 278 312 331 365 1095
Size: 278 34 19 34 730
Fragments arranged by size:
730 278 34 34 19
BciVI GTATCCnnnnn_n'
Cuts at: 0 614 1095
Size: 614 481
BfrBI ATG'CAT
Cuts at: 0 813 1095
Size: 813 282
BglI GCCn_nnn'nGGC
Cuts at: 0 86 358 782 1095
Size: 86 272 424 313
Fragments arranged by size:
424 313 272 86
Bme1580I G_kGCm'C
Cuts at: 0 190 196 231 340 1019 1070 1095
Size: 190 6 35 109 679 51 25
Fragments arranged by size:
679 190 109 51 35 25 6
BmgBI CAC'GTC
Cuts at: 0 315 419 1095
Size: 315 104 676
Fragments arranged by size:
676 315 104
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 969 1081 1095
Size: 195 774 112 14
Fragments arranged by size:
774 195 112 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1019 1095
Size: 1019 76
BsaI GGTCTCn'nnnn_
Cuts at: 0 997 1095
Size: 997 98
BsaHI Gr'CG_yC
Cuts at: 0 133 1095
Size: 133 962
BsaWI w'CCGG_w
Cuts at: 0 617 1095
Size: 617 478
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 233 263 397 427 1095
Size: 233 30 134 30 668
Fragments arranged by size:
668 233 134 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 241 1095
Size: 241 854
BseYI C'CCAG_C
Cuts at: 0 108 237 364 674 780 1070 1095
Size: 108 129 127 310 106 290 25
Fragments arranged by size:
310 290 129 127 108 106 25
BsiEI CG_ry'CG
Cuts at: 0 9 284 390 1095
Size: 9 275 106 705
Fragments arranged by size:
705 275 106 9
BsiHKAI G_wGCw'C
Cuts at: 0 294 608 758 1019 1035 1095
Size: 294 314 150 261 16 60
Fragments arranged by size:
314 294 261 150 60 16
BsmI GAATG_Cn'
Cuts at: 0 544 1095
Size: 544 551
BsmBI CGTCTCn'nnnn_
Cuts at: 0 417 1095
Size: 417 678
Bsp1286I G_dGCh'C
Cuts at: 0 76 190 196 231 294 340 347 608
Size: 76 114 6 35 63 46 7 261
Cuts at: 608 758 787 880 949 1019 1035 1070 1095
Size: 150 29 93 69 70 16 35 25
Fragments arranged by size:
261 150 114 93 76 70 69 63
46 35 35 29 25 16 7 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 113 1095
Size: 113 982
BsrBI CCG'CTC
Cuts at: 0 286 1095
Size: 286 809
BsrFI r'CCGG_y
Cuts at: 0 4 129 276 1095
Size: 4 125 147 819
Fragments arranged by size:
819 147 125 4
BssHII G'CGCG_C
Cuts at: 0 791 1095
Size: 791 304
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1059 1095
Size: 1059 36
BstYI r'GATC_y
Cuts at: 0 154 1095
Size: 154 941
BtgI C'CryG_G
Cuts at: 0 719 824 1052 1095
Size: 719 105 228 43
Fragments arranged by size:
719 228 105 43
BtsI GCAGTG_nn'
Cuts at: 0 512 729 1095
Size: 512 217 366
Fragments arranged by size:
512 366 217
EaeI y'GGCC_r
Cuts at: 0 6 281 387 695 1095
Size: 6 275 106 308 400
Fragments arranged by size:
400 308 275 106 6
EagI C'GGCC_G
Cuts at: 0 6 281 387 1095
Size: 6 275 106 708
Fragments arranged by size:
708 275 106 6
EarI CTCTTCn'nnn_
Cuts at: 0 524 1095
Size: 524 571
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 362 753 1095
Size: 362 391 342
Fragments arranged by size:
391 362 342
EcoICRI GAG'CTC
Cuts at: 0 606 1095
Size: 606 489
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 461 969 1081 1095
Size: 195 266 508 112 14
Fragments arranged by size:
508 266 195 112 14
EcoO109I rG'GnC_Cy
Cuts at: 0 227 228 336 337 1066 1067 1095
Size: 227 1 108 1 729 1 28
Fragments arranged by size:
729 227 108 28 1 1 1
HaeII r_GCGC'y
Cuts at: 0 49 136 675 1095
Size: 49 87 539 420
Fragments arranged by size:
539 420 87 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 233 265 397 421 429 453 904 936
Size: 233 32 132 24 8 24 451 32
Cuts at: 936 1095
Size: 159
Fragments arranged by size:
451 233 159 132 32 32 24 24
8
KasI G'GCGC_C
Cuts at: 0 132 1095
Size: 132 963
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 515 1070 1095
Size: 47 468 555 25
Fragments arranged by size:
555 468 47 25
MslI CAynn'nnrTG
Cuts at: 0 829 1095
Size: 829 266
MspA1I CmG'CkG
Cuts at: 0 139 237 349 357 525 741 1095
Size: 139 98 112 8 168 216 354
Fragments arranged by size:
354 216 168 139 112 98 8
NaeI GCC'GGC
Cuts at: 0 131 278 1095
Size: 131 147 817
Fragments arranged by size:
817 147 131
NarI GG'CG_CC
Cuts at: 0 133 1095
Size: 133 962
NcoI C'CATG_G
Cuts at: 0 719 824 1095
Size: 719 105 271
Fragments arranged by size:
719 271 105
NgoMIV G'CCGG_C
Cuts at: 0 129 276 1095
Size: 129 147 819
Fragments arranged by size:
819 147 129
NotI GC'GGCC_GC
Cuts at: 0 281 1095
Size: 281 814
NsiI A_TGCA'T
Cuts at: 0 815 1095
Size: 815 280
PasI CC'CwG_GG
Cuts at: 0 332 1021 1095
Size: 332 689 74
Fragments arranged by size:
689 332 74
PspOMI G'GGCC_C
Cuts at: 0 192 227 336 1066 1095
Size: 192 35 109 730 29
Fragments arranged by size:
730 192 109 35 29
PspXI vC'TCGA_Gb
Cuts at: 0 287 1095
Size: 287 808
PvuII CAG'CTG
Cuts at: 0 525 1095
Size: 525 570
SacI G_AGCT'C
Cuts at: 0 608 1095
Size: 608 487
SfoI GGC'GCC
Cuts at: 0 134 1095
Size: 134 961
SgrAI Cr'CCGG_yG
Cuts at: 0 276 1095
Size: 276 819
SmaI CCC'GGG
Cuts at: 0 85 191 197 1095
Size: 85 106 6 898
Fragments arranged by size:
898 106 85 6
SmlI C'TyrA_G
Cuts at: 0 287 1034 1095
Size: 287 747 61
Fragments arranged by size:
747 287 61
SrfI GCCC'GGGC
Cuts at: 0 191 1095
Size: 191 904
StyI C'CwwG_G
Cuts at: 0 67 473 719 824 1095
Size: 67 406 246 105 271
Fragments arranged by size:
406 271 246 105 67
Tth111I GACn'n_nGTC
Cuts at: 0 409 1095
Size: 409 686
XhoI C'TCGA_G
Cuts at: 0 287 1095
Size: 287 808
XmaI C'CCGG_G
Cuts at: 0 83 189 195 1095
Size: 83 106 6 900
Fragments arranged by size:
900 106 83 6
XmnI GAAnn'nnTTC
Cuts at: 0 544 1095
Size: 544 551
Enzymes that do cut:
AcuI AfeI AhdI AlfI AloI ApaI ApaLI AvaI
AvrII BanI BanII BbeI BbsI BcgI BciVI BfrBI
BglI Bme1580I BmgBI BpmI BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrFI BssHII BstXI BstYI BtgI BtsI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII
Hin4I KasI MmeI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NsiI PasI PspOMI PspXI PvuII SacI
SfoI SgrAI SmaI SmlI SrfI StyI Tth111I XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AleI AlwNI ApoI AscI AseI AsiSI BaeI BamHI
BbvCI BclI BglII BlpI BmrI BmtI BplI Bpu10I
BsaAI BsaBI BsgI BsiWI BspEI BspHI BsrDI BsrGI
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MscI NdeI NheI NruI NspI PacI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SgfI SnaBI SpeI
SphI SspI StuI SwaI TaqII TatI TstI XbaI
XcmI ZraI