Restriction map for
pFN21AB7924
(Linear) (Six-base) MAPSORT of: KIBB7924.seq Check: 5634 from: 1 to: 1038
KIBB7924.orf
With 182 enzymes: SgfI *
July 15, 2010 18:31 ..
Acc65I G'GTAC_C
Cuts at: 0 597 1038
Size: 597 441
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 863 1038
Size: 445 418 175
Fragments arranged by size:
445 418 175
AflII C'TTAA_G
Cuts at: 0 942 1038
Size: 942 96
AflIII A'CryG_T
Cuts at: 0 825 929 1038
Size: 825 104 109
Fragments arranged by size:
825 109 104
AlwNI CAG_nnn'CTG
Cuts at: 0 849 1038
Size: 849 189
ApoI r'AATT_y
Cuts at: 0 269 1038
Size: 269 769
AseI AT'TA_AT
Cuts at: 0 497 1038
Size: 497 541
AvaI C'yCGr_G
Cuts at: 0 24 121 1038
Size: 24 97 917
Fragments arranged by size:
917 97 24
BanI G'GyrC_C
Cuts at: 0 36 127 573 597 1038
Size: 36 91 446 24 441
Fragments arranged by size:
446 441 91 36 24
BanII G_rGCy'C
Cuts at: 0 17 25 1038
Size: 17 8 1013
Fragments arranged by size:
1013 17 8
BbeI G_GCGC'C
Cuts at: 0 40 131 1038
Size: 40 91 907
Fragments arranged by size:
907 91 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 533 914 1038
Size: 533 381 124
Fragments arranged by size:
533 381 124
BciVI GTATCCnnnnn_n'
Cuts at: 0 343 1038
Size: 343 695
BclI T'GATC_A
Cuts at: 0 441 1038
Size: 441 597
BglI GCCn_nnn'nGGC
Cuts at: 0 45 571 1038
Size: 45 526 467
Fragments arranged by size:
526 467 45
BglII A'GATC_T
Cuts at: 0 414 1038
Size: 414 624
BmgBI CAC'GTC
Cuts at: 0 930 1038
Size: 930 108
BmrI ACTGGGnnnn_n'
Cuts at: 0 756 1038
Size: 756 282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 758 1038
Size: 613 145 280
Fragments arranged by size:
613 280 145
Bpu10I CC'TnA_GC
Cuts at: 0 818 1038
Size: 818 220
BsaHI Gr'CG_yC
Cuts at: 0 37 128 1038
Size: 37 91 910
Fragments arranged by size:
910 91 37
BsiEI CG_ry'CG
Cuts at: 0 67 1038
Size: 67 971
BsiHKAI G_wGCw'C
Cuts at: 0 25 1038
Size: 25 1013
Bsp1286I G_dGCh'C
Cuts at: 0 17 25 1038
Size: 17 8 1013
Fragments arranged by size:
1013 17 8
BspHI T'CATG_A
Cuts at: 0 522 1038
Size: 522 516
BsrFI r'CCGG_y
Cuts at: 0 39 1038
Size: 39 999
BssSI C'ACGA_G
Cuts at: 0 151 1038
Size: 151 887
BstYI r'GATC_y
Cuts at: 0 222 414 886 1038
Size: 222 192 472 152
Fragments arranged by size:
472 222 192 152
BtgI C'CryG_G
Cuts at: 0 713 1038
Size: 713 325
EaeI y'GGCC_r
Cuts at: 0 64 563 1038
Size: 64 499 475
Fragments arranged by size:
499 475 64
EagI C'GGCC_G
Cuts at: 0 64 1038
Size: 64 974
EarI CTCTTCn'nnn_
Cuts at: 0 151 1038
Size: 151 887
EcoICRI GAG'CTC
Cuts at: 0 23 1038
Size: 23 1015
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 613 758 863 1038
Size: 445 168 145 105 175
Fragments arranged by size:
445 175 168 145 105
EcoO109I rG'GnC_Cy
Cuts at: 0 664 1038
Size: 664 374
EcoRV GAT'ATC
Cuts at: 0 279 394 903 1038
Size: 279 115 509 135
Fragments arranged by size:
509 279 135 115
HaeII r_GCGC'y
Cuts at: 0 40 131 1038
Size: 40 91 907
Fragments arranged by size:
907 91 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 904 936 1038
Size: 904 32 102
Fragments arranged by size:
904 102 32
KasI G'GCGC_C
Cuts at: 0 36 127 1038
Size: 36 91 911
Fragments arranged by size:
911 91 36
KpnI G_GTAC'C
Cuts at: 0 601 1038
Size: 601 437
MscI TGG'CCA
Cuts at: 0 565 1038
Size: 565 473
MspA1I CmG'CkG
Cuts at: 0 63 1038
Size: 63 975
NaeI GCC'GGC
Cuts at: 0 41 1038
Size: 41 997
NarI GG'CG_CC
Cuts at: 0 37 128 1038
Size: 37 91 910
Fragments arranged by size:
910 91 37
NgoMIV G'CCGG_C
Cuts at: 0 39 1038
Size: 39 999
NotI GC'GGCC_GC
Cuts at: 0 64 1038
Size: 64 974
NspI r_CATG'y
Cuts at: 0 829 1038
Size: 829 209
PciI A'CATG_T
Cuts at: 0 825 1038
Size: 825 213
PpuMI rG'GwC_Cy
Cuts at: 0 664 1038
Size: 664 374
PsiI TTA'TAA
Cuts at: 0 685 1038
Size: 685 353
PstI C_TGCA'G
Cuts at: 0 200 854 1038
Size: 200 654 184
Fragments arranged by size:
654 200 184
SacI G_AGCT'C
Cuts at: 0 25 1038
Size: 25 1013
SapI GCTCTTCn'nnn_
Cuts at: 0 151 1038
Size: 151 887
SfcI C'TryA_G
Cuts at: 0 196 460 850 866 1038
Size: 196 264 390 16 172
Fragments arranged by size:
390 264 196 172 16
SfoI GGC'GCC
Cuts at: 0 38 129 1038
Size: 38 91 909
Fragments arranged by size:
909 91 38
SmlI C'TyrA_G
Cuts at: 0 942 1038
Size: 942 96
StyI C'CwwG_G
Cuts at: 0 206 1038
Size: 206 832
XbaI T'CTAG_A
Cuts at: 0 417 1038
Size: 417 621
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 609 1038
Size: 609 429
Enzymes that do cut:
Acc65I AcuI AflII AflIII AlwNI ApoI AseI AvaI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BmgBI BmrI BpmI Bpu10I BsaHI BsiEI BsiHKAI Bsp1286I
BspHI BsrFI BssSI BstYI BtgI EaeI EagI EarI
EcoICRI Eco57MI EcoO109I EcoRV HaeII Hin4I KasI KpnI
MscI MspA1I NaeI NarI NgoMIV NotI NspI PciI
PpuMI PsiI PstI SacI SapI SfcI SfoI SmlI
StyI XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvrII BaeI
BamHI BbvCI BcgI BfrBI BlpI Bme1580I BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrDI
BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EciI
EcoNI EcoRI FalI FseI FspI FspAI HincII HindIII
HpaI MfeI MluI MmeI MslI NcoI NdeI NheI
NruI NsiI PacI PasI PflMI PfoI PmeI PmlI
PpiI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI TstI Tth111I XhoI XmaI XmnI
ZraI