Restriction map for
pFN21AB7912
(Linear) (Six-base) MAPSORT of: KIBB7912.seq Check: 3184 from: 1 to: 993
KIBB7912.orf
With 182 enzymes: SgfI *
March 22, 2012 15:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 797 993
Size: 797 196
AfeI AGC'GCT
Cuts at: 0 765 993
Size: 765 228
AlwNI CAG_nnn'CTG
Cuts at: 0 528 993
Size: 528 465
AvaI C'yCGr_G
Cuts at: 0 545 993
Size: 545 448
BanI G'GyrC_C
Cuts at: 0 298 993
Size: 298 695
BbeI G_GCGC'C
Cuts at: 0 302 993
Size: 302 691
BglI GCCn_nnn'nGGC
Cuts at: 0 865 993
Size: 865 128
BmrI ACTGGGnnnn_n'
Cuts at: 0 538 993
Size: 538 455
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 281 484 921 993
Size: 106 175 203 437 72
Fragments arranged by size:
437 203 175 106 72
BsaI GGTCTCn'nnnn_
Cuts at: 0 475 993
Size: 475 518
BsaAI yAC'GTr
Cuts at: 0 71 993
Size: 71 922
BsaHI Gr'CG_yC
Cuts at: 0 299 497 721 993
Size: 299 198 224 272
Fragments arranged by size:
299 272 224 198
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 215 245 993
Size: 215 30 748
Fragments arranged by size:
748 215 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 209 230 239 251 254 257 269 278
Size: 209 21 9 12 3 3 12 9
Cuts at: 278 993
Size: 715
Fragments arranged by size:
715 209 21 12 12 9 9 3
3
BseYI C'CCAG_C
Cuts at: 0 831 993
Size: 831 162
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 571 993
Size: 111 460 422
Fragments arranged by size:
460 422 111
BsiEI CG_ry'CG
Cuts at: 0 122 150 176 905 993
Size: 122 28 26 729 88
Fragments arranged by size:
729 122 88 28 26
BsmBI CGTCTCn'nnnn_
Cuts at: 0 27 993
Size: 27 966
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 543 797 993
Size: 543 254 196
Fragments arranged by size:
543 254 196
BsrBI CCG'CTC
Cuts at: 0 92 855 993
Size: 92 763 138
Fragments arranged by size:
763 138 92
BsrDI GCAATG_nn'
Cuts at: 0 708 993
Size: 708 285
BsrFI r'CCGG_y
Cuts at: 0 121 175 993
Size: 121 54 818
Fragments arranged by size:
818 121 54
BtgI C'CryG_G
Cuts at: 0 869 993
Size: 869 124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 927 993
Size: 927 66
DrdI GACnn_nn'nnGTC
Cuts at: 0 345 726 993
Size: 345 381 267
Fragments arranged by size:
381 345 267
EaeI y'GGCC_r
Cuts at: 0 173 803 866 902 993
Size: 173 630 63 36 91
Fragments arranged by size:
630 173 91 63 36
EagI C'GGCC_G
Cuts at: 0 173 902 993
Size: 173 729 91
Fragments arranged by size:
729 173 91
EarI CTCTTCn'nnn_
Cuts at: 0 567 993
Size: 567 426
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 194 206 215 227 236 266 873 993
Size: 194 12 9 12 9 30 607 120
Fragments arranged by size:
607 194 120 30 12 12 9 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 281 484 921 993
Size: 106 175 203 437 72
Fragments arranged by size:
437 203 175 106 72
EcoNI CCTnn'n_nnAGG
Cuts at: 0 538 993
Size: 538 455
HaeII r_GCGC'y
Cuts at: 0 273 302 767 993
Size: 273 29 465 226
Fragments arranged by size:
465 273 226 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 411 443 474 506 839 871 993
Size: 411 32 31 32 333 32 122
Fragments arranged by size:
411 333 122 32 32 32 31
HincII GTy'rAC
Cuts at: 0 669 993
Size: 669 324
HindIII A'AGCT_T
Cuts at: 0 644 993
Size: 644 349
KasI G'GCGC_C
Cuts at: 0 298 993
Size: 298 695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 518 993
Size: 409 109 475
Fragments arranged by size:
475 409 109
MslI CAynn'nnrTG
Cuts at: 0 611 993
Size: 611 382
MspA1I CmG'CkG
Cuts at: 0 99 750 756 871 993
Size: 99 651 6 115 122
Fragments arranged by size:
651 122 115 99 6
NaeI GCC'GGC
Cuts at: 0 177 993
Size: 177 816
NarI GG'CG_CC
Cuts at: 0 299 993
Size: 299 694
NgoMIV G'CCGG_C
Cuts at: 0 175 993
Size: 175 818
NotI GC'GGCC_GC
Cuts at: 0 902 993
Size: 902 91
PasI CC'CwG_GG
Cuts at: 0 984 993
Size: 984 9
PflMI CCAn_nnn'nTGG
Cuts at: 0 528 993
Size: 528 465
PfoI T'CCnGG_A
Cuts at: 0 281 993
Size: 281 712
PstI C_TGCA'G
Cuts at: 0 755 993
Size: 755 238
PvuII CAG'CTG
Cuts at: 0 750 756 993
Size: 750 6 237
Fragments arranged by size:
750 237 6
SacII CC_GC'GG
Cuts at: 0 872 993
Size: 872 121
SapI GCTCTTCn'nnn_
Cuts at: 0 567 993
Size: 567 426
SfcI C'TryA_G
Cuts at: 0 751 993
Size: 751 242
SfoI GGC'GCC
Cuts at: 0 300 993
Size: 300 693
StyI C'CwwG_G
Cuts at: 0 110 993
Size: 110 883
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 441 993
Size: 441 552
Enzymes that do cut:
AarI AfeI AlwNI AvaI BanI BbeI BglI BmrI
BpmI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsmBI BspMI BsrBI BsrDI BsrFI BtgI BtgZI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI HaeII
Hin4I HincII HindIII KasI MmeI MslI MspA1I NaeI
NarI NgoMIV NotI PasI PflMI PfoI PstI PvuII
SacII SapI SfcI SfoI StyI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmtI BplI Bpu10I BpuEI BsaBI BsaWI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtsI ClaI
CspCI DraI DraIII EcoICRI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HpaI KpnI MfeI MluI MscI
NcoI NdeI NheI NruI NsiI NspI PacI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SalI SanDI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI