(Linear) (Six-base) MAPSORT of: KIBB7903.seq  Check: 5761  from: 1  to: 321



KIBB7903.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:31  ..

AfeI AGC'GCT

Cuts at:      0     119     321 
   Size:        119     202
 
AvaI C'yCGr_G

Cuts at:      0     109     170     321 
   Size:        109      61     151

  Fragments arranged by size: 

                 151     109      61
 
BanII G_rGCy'C

Cuts at:      0      16     143     321 
   Size:         16     127     178

  Fragments arranged by size: 

                 178     127      16
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      30     309     321 
   Size:         30     279      12

  Fragments arranged by size: 

                 279      30      12
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     218     289     321 
   Size:        218      71      32

  Fragments arranged by size: 

                 218      71      32
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     159     321 
   Size:        159     162
 
BsaWI w'CCGG_w

Cuts at:      0      99     321 
   Size:         99     222
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     216     246     321 
   Size:        216      30      75

  Fragments arranged by size: 

                 216      75      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     298     321 
   Size:        298      23
 
BseYI C'CCAG_C

Cuts at:      0     142     321 
   Size:        142     179
 
BsiHKAI G_wGCw'C

Cuts at:      0      16     321 
   Size:         16     305
 
Bsp1286I G_dGCh'C

Cuts at:      0      16     143     321 
   Size:         16     127     178

  Fragments arranged by size: 

                 178     127      16
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     267     321 
   Size:        267      54
 
EarI CTCTTCn'nnn_

Cuts at:      0     275     321 
   Size:        275      46
 
EcoICRI GAG'CTC

Cuts at:      0      14     321 
   Size:         14     307
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     218     289     321 
   Size:        218      71      32

  Fragments arranged by size: 

                 218      71      32
 
HaeII r_GCGC'y

Cuts at:      0     121     321 
   Size:        121     200
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      33     321 
   Size:         33     288
 
PfoI T'CCnGG_A

Cuts at:      0     234     321 
   Size:        234      87
 
SacI G_AGCT'C

Cuts at:      0      16     321 
   Size:         16     305
 
SmaI CCC'GGG

Cuts at:      0     111     321 
   Size:        111     210
 
StyI C'CwwG_G

Cuts at:      0      32     247     321 
   Size:         32     215      74

  Fragments arranged by size: 

                 215      74      32
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     216     248     321 
   Size:        216      32      73

  Fragments arranged by size: 

                 216      73      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      30     321 
   Size:         30     291
 
XmaI C'CCGG_G

Cuts at:      0     109     321 
   Size:        109     212
 

 Enzymes that do cut:

     AfeI     AvaI    BanII     BplI     BpmI     BsaI    BsaWI    BsaXI
    BseRI    BseYI  BsiHKAI Bsp1286I    BstXI     EarI  EcoICRI  Eco57MI
    HaeII    PflMI     PfoI     SacI     SmaI     StyI     TstI     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI  Tth111I     XbaI
     XhoI     XmnI     ZraI